Nerve growth factor enhances nerve regeneration through fibronectin grafts
Soluble fibronectin and nerve growth factor (NGF) promote axonal regeneration when placed in silicone tubes. We investigated the ability of orientated fibronectin mats to bind and release bioactive NGF and the possibility of augmenting axonal regeneration following axotomy by using fibronectin condu...
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| Veröffentlicht in: | Journal of hand surgery, British volume British volume, 1996-08, Vol.21 (4), p.514-522 |
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| container_title | Journal of hand surgery, British volume |
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| creator | Whitworth, I.H. Brown, R.A. Doré, C.J. Anand, P. Green, C.J. Terenghi, G. |
| description | Soluble fibronectin and nerve growth factor (NGF) promote axonal regeneration when placed in silicone tubes. We investigated the ability of orientated fibronectin mats to bind and release bioactive NGF and the possibility of augmenting axonal regeneration following axotomy by using fibronectin conduits impregnated with NGF. The release of NGF was quantified using a fluorometric ELISA and bioactivity confirmed with a neuronal culture bioassay. Immunohistochemical techniques and computerized image analysis were used to assess the rate and volume of axonal and Schwann cell regeneration. The delivery of NGF to the site of injury produced an increase in the rate (
P≤0.007) and volume (
P≤0.004) of both axonal and Schwann cell regeneration when compared to conduits of plain fibronectin. We conclude that the local delivery of NGF by impregnated fibronectin conduits enhances axonal regeneration. |
| doi_str_mv | 10.1016/S0266-7681(96)80058-1 |
| format | Article |
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P≤0.007) and volume (
P≤0.004) of both axonal and Schwann cell regeneration when compared to conduits of plain fibronectin. We conclude that the local delivery of NGF by impregnated fibronectin conduits enhances axonal regeneration.</description><identifier>ISSN: 0266-7681</identifier><identifier>EISSN: 1532-2211</identifier><identifier>DOI: 10.1016/S0266-7681(96)80058-1</identifier><identifier>PMID: 8856546</identifier><identifier>CODEN: JHASE4</identifier><language>eng</language><publisher>London, England: Elsevier Ltd</publisher><subject>Animals ; Axons - drug effects ; Axons - pathology ; Biological and medical sciences ; Calcitonin Gene-Related Peptide - analysis ; Chick Embryo ; Cranial nerves. Peripheral nerves. Autonomic nervous system ; Culture Techniques ; Drug Implants ; Fibronectins ; Ganglia, Spinal - drug effects ; Ganglia, Spinal - pathology ; Immunoenzyme Techniques ; Medical sciences ; Nerve Fibers, Myelinated - drug effects ; Nerve Fibers, Myelinated - pathology ; Nerve Growth Factors - pharmacology ; Nerve Regeneration - drug effects ; Neurosurgery ; Rats ; Rats, Inbred Lew ; Schwann Cells - drug effects ; Schwann Cells - pathology ; Sciatic Nerve - drug effects ; Sciatic Nerve - pathology ; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</subject><ispartof>Journal of hand surgery, British volume, 1996-08, Vol.21 (4), p.514-522</ispartof><rights>1996 The British Society for Surgery of the Hand</rights><rights>1996 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c464t-6fa71b9f4a5603d92cb2ad1d777d06c733d6ba25a4adcf6c85c4ea6323b6e9e3</citedby><cites>FETCH-LOGICAL-c464t-6fa71b9f4a5603d92cb2ad1d777d06c733d6ba25a4adcf6c85c4ea6323b6e9e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://journals.sagepub.com/doi/pdf/10.1016/S0266-7681(96)80058-1$$EPDF$$P50$$Gsage$$H</linktopdf><linktohtml>$$Uhttps://journals.sagepub.com/doi/10.1016/S0266-7681(96)80058-1$$EHTML$$P50$$Gsage$$H</linktohtml><link.rule.ids>314,778,782,21806,27911,27912,43608,43609</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3161970$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8856546$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Whitworth, I.H.</creatorcontrib><creatorcontrib>Brown, R.A.</creatorcontrib><creatorcontrib>Doré, C.J.</creatorcontrib><creatorcontrib>Anand, P.</creatorcontrib><creatorcontrib>Green, C.J.</creatorcontrib><creatorcontrib>Terenghi, G.</creatorcontrib><title>Nerve growth factor enhances nerve regeneration through fibronectin grafts</title><title>Journal of hand surgery, British volume</title><addtitle>J Hand Surg Br</addtitle><description>Soluble fibronectin and nerve growth factor (NGF) promote axonal regeneration when placed in silicone tubes. We investigated the ability of orientated fibronectin mats to bind and release bioactive NGF and the possibility of augmenting axonal regeneration following axotomy by using fibronectin conduits impregnated with NGF. The release of NGF was quantified using a fluorometric ELISA and bioactivity confirmed with a neuronal culture bioassay. Immunohistochemical techniques and computerized image analysis were used to assess the rate and volume of axonal and Schwann cell regeneration. The delivery of NGF to the site of injury produced an increase in the rate (
P≤0.007) and volume (
P≤0.004) of both axonal and Schwann cell regeneration when compared to conduits of plain fibronectin. We conclude that the local delivery of NGF by impregnated fibronectin conduits enhances axonal regeneration.</description><subject>Animals</subject><subject>Axons - drug effects</subject><subject>Axons - pathology</subject><subject>Biological and medical sciences</subject><subject>Calcitonin Gene-Related Peptide - analysis</subject><subject>Chick Embryo</subject><subject>Cranial nerves. Peripheral nerves. Autonomic nervous system</subject><subject>Culture Techniques</subject><subject>Drug Implants</subject><subject>Fibronectins</subject><subject>Ganglia, Spinal - drug effects</subject><subject>Ganglia, Spinal - pathology</subject><subject>Immunoenzyme Techniques</subject><subject>Medical sciences</subject><subject>Nerve Fibers, Myelinated - drug effects</subject><subject>Nerve Fibers, Myelinated - pathology</subject><subject>Nerve Growth Factors - pharmacology</subject><subject>Nerve Regeneration - drug effects</subject><subject>Neurosurgery</subject><subject>Rats</subject><subject>Rats, Inbred Lew</subject><subject>Schwann Cells - drug effects</subject><subject>Schwann Cells - pathology</subject><subject>Sciatic Nerve - drug effects</subject><subject>Sciatic Nerve - pathology</subject><subject>Surgery (general aspects). Transplantations, organ and tissue grafts. 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Peripheral nerves. Autonomic nervous system</topic><topic>Culture Techniques</topic><topic>Drug Implants</topic><topic>Fibronectins</topic><topic>Ganglia, Spinal - drug effects</topic><topic>Ganglia, Spinal - pathology</topic><topic>Immunoenzyme Techniques</topic><topic>Medical sciences</topic><topic>Nerve Fibers, Myelinated - drug effects</topic><topic>Nerve Fibers, Myelinated - pathology</topic><topic>Nerve Growth Factors - pharmacology</topic><topic>Nerve Regeneration - drug effects</topic><topic>Neurosurgery</topic><topic>Rats</topic><topic>Rats, Inbred Lew</topic><topic>Schwann Cells - drug effects</topic><topic>Schwann Cells - pathology</topic><topic>Sciatic Nerve - drug effects</topic><topic>Sciatic Nerve - pathology</topic><topic>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Whitworth, I.H.</creatorcontrib><creatorcontrib>Brown, R.A.</creatorcontrib><creatorcontrib>Doré, C.J.</creatorcontrib><creatorcontrib>Anand, P.</creatorcontrib><creatorcontrib>Green, C.J.</creatorcontrib><creatorcontrib>Terenghi, G.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of hand surgery, British volume</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Whitworth, I.H.</au><au>Brown, R.A.</au><au>Doré, C.J.</au><au>Anand, P.</au><au>Green, C.J.</au><au>Terenghi, G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Nerve growth factor enhances nerve regeneration through fibronectin grafts</atitle><jtitle>Journal of hand surgery, British volume</jtitle><addtitle>J Hand Surg Br</addtitle><date>1996-08-01</date><risdate>1996</risdate><volume>21</volume><issue>4</issue><spage>514</spage><epage>522</epage><pages>514-522</pages><issn>0266-7681</issn><eissn>1532-2211</eissn><coden>JHASE4</coden><abstract>Soluble fibronectin and nerve growth factor (NGF) promote axonal regeneration when placed in silicone tubes. We investigated the ability of orientated fibronectin mats to bind and release bioactive NGF and the possibility of augmenting axonal regeneration following axotomy by using fibronectin conduits impregnated with NGF. The release of NGF was quantified using a fluorometric ELISA and bioactivity confirmed with a neuronal culture bioassay. Immunohistochemical techniques and computerized image analysis were used to assess the rate and volume of axonal and Schwann cell regeneration. The delivery of NGF to the site of injury produced an increase in the rate (
P≤0.007) and volume (
P≤0.004) of both axonal and Schwann cell regeneration when compared to conduits of plain fibronectin. We conclude that the local delivery of NGF by impregnated fibronectin conduits enhances axonal regeneration.</abstract><cop>London, England</cop><pub>Elsevier Ltd</pub><pmid>8856546</pmid><doi>10.1016/S0266-7681(96)80058-1</doi><tpages>9</tpages></addata></record> |
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| subjects | Animals Axons - drug effects Axons - pathology Biological and medical sciences Calcitonin Gene-Related Peptide - analysis Chick Embryo Cranial nerves. Peripheral nerves. Autonomic nervous system Culture Techniques Drug Implants Fibronectins Ganglia, Spinal - drug effects Ganglia, Spinal - pathology Immunoenzyme Techniques Medical sciences Nerve Fibers, Myelinated - drug effects Nerve Fibers, Myelinated - pathology Nerve Growth Factors - pharmacology Nerve Regeneration - drug effects Neurosurgery Rats Rats, Inbred Lew Schwann Cells - drug effects Schwann Cells - pathology Sciatic Nerve - drug effects Sciatic Nerve - pathology Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases |
| title | Nerve growth factor enhances nerve regeneration through fibronectin grafts |
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