Different Oligomeric States are Involved in the Allosteric Behavior of Uracil Phosphoribosyltransferase from Escherichia Coli

Uracil phosphoribosyltransferase, catalyzing the formation of UMP and pyrophosphate from uracil and 5‐phosphoribosyl‐α‐1‐diphosphate (PP RibP), was purified from an overproducing strain of Escherichia coli. GTP was shown to activate the enzyme by reducing Km for PP RibP by about fivefold without aff...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	European journal of biochemistry 1996-09, Vol.240 (3), p.637-645
Hauptverfasser:	Jensen, Kaj Frank, Mygind, Bente
Format:	Artikel
Sprache:	eng
Schlagworte:	5‐phosphoribosyl 1‐diphosphate Allosteric Regulation Base Sequence Cations, Divalent - pharmacology Centrifugation, Density Gradient Chromatography, Gel DNA Primers - genetics Enzyme Activation - drug effects Escherichia coli - enzymology Escherichia coli - genetics GTP activation Guanosine Tetraphosphate - pharmacology Guanosine Triphosphate - pharmacology Kinetics Ligands Models, Molecular Pentosyltransferases - chemistry Pentosyltransferases - genetics Pentosyltransferases - metabolism Phosphoribosyl Pyrophosphate - pharmacology Protein Conformation pyrimidine biosynthesis pyrimidine salvage stringent response UMP
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!