Surface adhesion‐mediated regulation of chondrocyte‐specific gene expression in the nontransformed RCJ 3.1c5.18 rat chondrocyte cell line
Recent evidence suggests that decreased chondrocyte function in osteoarthritis and other articular disorders may be due to chondrocyte dedifferentiation produced by altered regulatory signals from the cartilage extracellular matrix (ECM). However, there are currently no mammalian chondrocytic cell l...
Gespeichert in:
| Veröffentlicht in: | Journal of bone and mineral research 1996-08, Vol.11 (8), p.1130-1138 |
|---|---|
| Hauptverfasser: | , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1138 |
|---|---|
| container_issue | 8 |
| container_start_page | 1130 |
| container_title | Journal of bone and mineral research |
| container_volume | 11 |
| creator | Mcdougall, Skye Fu, Y‐Hsien Lowe, Gina N. Williams, Alexia Polendo, Ruben Benya, Paul D. Iida‐Klein, Akiko Fang, Meika A. Hahn, Theodore J. |
| description | Recent evidence suggests that decreased chondrocyte function in osteoarthritis and other articular disorders may be due to chondrocyte dedifferentiation produced by altered regulatory signals from the cartilage extracellular matrix (ECM). However, there are currently no mammalian chondrocytic cell line systems adapted to the study of this process. We therefore examined the effects of ECM growth conditions on markers of differentiated chondrocytic phenotype expression in the nontransformed rat RCJ 3.1C5.18 (RCJ) chondrocyte cell line, including type II collagen expression, aggrecan production, link protein gene expression, and parathyroid hormone (PTH) receptor number. RCJ cells grown in monolayer on plastic exhibited a dedifferentiated phenotype characterized by flattened cell morphology, with >80% type I collagen and |
| doi_str_mv | 10.1002/jbmr.5650110812 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_78412291</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>78412291</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4042-55ee25d9d0fba874f508b69d4073da219c2f39ec7e3b571d84bc932620a1ba273</originalsourceid><addsrcrecordid>eNqFkU1v1DAQhi1EVZbCmROSD4hbtv6OI06w4qNVEVKBc-TY466rrL3YiWBv_AEkfiO_hKS7anvraaSZZ955NS9CLyhZUkLY6XW3yUupJKGUaMoeoQWVjFdCafoYLYjWoiKC0yfoaSnXhBAllTpGx1pLwUSzQH--jtkbC9i4NZSQ4r_ffzfgghnA4QxXY2-GqYuTx3adosvJ7gaYoLIFG3yw-AoiYPi1zVDmfRwiHtaAY4pDNrH4lCc9fLk6x3xJrVxSjbMZ7qthC32P-xDhGTrypi_w_FBP0PcP77-tPlUXXz6erd5eVFYQwSopAZh0jSO-M7oWXhLdqcYJUnNnGG0s87wBWwPvZE2dFp1tOFOMGNoZVvMT9Hqvu83pxwhlaDehzC5MhDSWttaCMtbQB0EqaykVmcHTPWhzKiWDb7c5bEzetZS0c1LtnFR7l9S08fIgPXbTh275QzTT_NVhboo1vZ-eaUO5xThtlLrB3uyxn6GH3UNX2_N3ny_vmfgPg1iwlA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>15755601</pqid></control><display><type>article</type><title>Surface adhesion‐mediated regulation of chondrocyte‐specific gene expression in the nontransformed RCJ 3.1c5.18 rat chondrocyte cell line</title><source>MEDLINE</source><source>Oxford Academic Journals (OUP)</source><creator>Mcdougall, Skye ; Fu, Y‐Hsien ; Lowe, Gina N. ; Williams, Alexia ; Polendo, Ruben ; Benya, Paul D. ; Iida‐Klein, Akiko ; Fang, Meika A. ; Hahn, Theodore J.</creator><creatorcontrib>Mcdougall, Skye ; Fu, Y‐Hsien ; Lowe, Gina N. ; Williams, Alexia ; Polendo, Ruben ; Benya, Paul D. ; Iida‐Klein, Akiko ; Fang, Meika A. ; Hahn, Theodore J.</creatorcontrib><description>Recent evidence suggests that decreased chondrocyte function in osteoarthritis and other articular disorders may be due to chondrocyte dedifferentiation produced by altered regulatory signals from the cartilage extracellular matrix (ECM). However, there are currently no mammalian chondrocytic cell line systems adapted to the study of this process. We therefore examined the effects of ECM growth conditions on markers of differentiated chondrocytic phenotype expression in the nontransformed rat RCJ 3.1C5.18 (RCJ) chondrocyte cell line, including type II collagen expression, aggrecan production, link protein gene expression, and parathyroid hormone (PTH) receptor number. RCJ cells grown in monolayer on plastic exhibited a dedifferentiated phenotype characterized by flattened cell morphology, with >80% type I collagen and <5% type II collagen production, as determined by two‐dimensional gel mapping electrophoresis of collagen cyanogen bromide peptides. In addition, aggrecan production was low, and link protein mRNA was not expressed at detectable levels. After transfer to growth under minimal attachment conditions on the surface of a composite type I collagen/agarose (0.15%–0.8%) gel (CAG) for 7 days, RCJ cells developed a rounded, chondrocytic morphology and a pattern of differentiated, chondrocytic gene expression, with 79% type II and 8% type I collagen production. Steady‐state type I and type II procollagen mRNA levels were altered in parallel with collagen protein expression. In cells grown on CAG, aggrecan production increased 6‐fold, and there was a marked increase in both aggrecan core protein and link protein mRNA levels. In addition, maximal PTH‐stimulated cAMP generation increased 15‐fold in association with an increased PTH receptor number. Therefore, the RCJ chondrocyte cell line is highly sensitive to ECM regulation of chondrocyte‐specific gene expression.</description><identifier>ISSN: 0884-0431</identifier><identifier>EISSN: 1523-4681</identifier><identifier>DOI: 10.1002/jbmr.5650110812</identifier><identifier>PMID: 8854249</identifier><identifier>CODEN: JBMREJ</identifier><language>eng</language><publisher>Washington, DC: John Wiley and Sons and The American Society for Bone and Mineral Research (ASBMR)</publisher><subject>Animals ; Biological and medical sciences ; Cartilage - cytology ; Cartilage - metabolism ; Cartilage - ultrastructure ; Cell Adhesion - physiology ; Cell Differentiation - physiology ; Cell Line ; Cell Line, Transformed ; Collagen - biosynthesis ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation - physiology ; Procollagen - genetics ; Rats ; Receptors, Parathyroid Hormone - analysis ; RNA, Messenger - biosynthesis ; Skeleton and joints ; Stem Cells - cytology ; Stem Cells - metabolism ; Vertebrates: osteoarticular system, musculoskeletal system</subject><ispartof>Journal of bone and mineral research, 1996-08, Vol.11 (8), p.1130-1138</ispartof><rights>Copyright © 1996 ASBMR</rights><rights>1996 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4042-55ee25d9d0fba874f508b69d4073da219c2f39ec7e3b571d84bc932620a1ba273</citedby><cites>FETCH-LOGICAL-c4042-55ee25d9d0fba874f508b69d4073da219c2f39ec7e3b571d84bc932620a1ba273</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3196649$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8854249$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mcdougall, Skye</creatorcontrib><creatorcontrib>Fu, Y‐Hsien</creatorcontrib><creatorcontrib>Lowe, Gina N.</creatorcontrib><creatorcontrib>Williams, Alexia</creatorcontrib><creatorcontrib>Polendo, Ruben</creatorcontrib><creatorcontrib>Benya, Paul D.</creatorcontrib><creatorcontrib>Iida‐Klein, Akiko</creatorcontrib><creatorcontrib>Fang, Meika A.</creatorcontrib><creatorcontrib>Hahn, Theodore J.</creatorcontrib><title>Surface adhesion‐mediated regulation of chondrocyte‐specific gene expression in the nontransformed RCJ 3.1c5.18 rat chondrocyte cell line</title><title>Journal of bone and mineral research</title><addtitle>J Bone Miner Res</addtitle><description>Recent evidence suggests that decreased chondrocyte function in osteoarthritis and other articular disorders may be due to chondrocyte dedifferentiation produced by altered regulatory signals from the cartilage extracellular matrix (ECM). However, there are currently no mammalian chondrocytic cell line systems adapted to the study of this process. We therefore examined the effects of ECM growth conditions on markers of differentiated chondrocytic phenotype expression in the nontransformed rat RCJ 3.1C5.18 (RCJ) chondrocyte cell line, including type II collagen expression, aggrecan production, link protein gene expression, and parathyroid hormone (PTH) receptor number. RCJ cells grown in monolayer on plastic exhibited a dedifferentiated phenotype characterized by flattened cell morphology, with >80% type I collagen and <5% type II collagen production, as determined by two‐dimensional gel mapping electrophoresis of collagen cyanogen bromide peptides. In addition, aggrecan production was low, and link protein mRNA was not expressed at detectable levels. After transfer to growth under minimal attachment conditions on the surface of a composite type I collagen/agarose (0.15%–0.8%) gel (CAG) for 7 days, RCJ cells developed a rounded, chondrocytic morphology and a pattern of differentiated, chondrocytic gene expression, with 79% type II and 8% type I collagen production. Steady‐state type I and type II procollagen mRNA levels were altered in parallel with collagen protein expression. In cells grown on CAG, aggrecan production increased 6‐fold, and there was a marked increase in both aggrecan core protein and link protein mRNA levels. In addition, maximal PTH‐stimulated cAMP generation increased 15‐fold in association with an increased PTH receptor number. Therefore, the RCJ chondrocyte cell line is highly sensitive to ECM regulation of chondrocyte‐specific gene expression.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cartilage - cytology</subject><subject>Cartilage - metabolism</subject><subject>Cartilage - ultrastructure</subject><subject>Cell Adhesion - physiology</subject><subject>Cell Differentiation - physiology</subject><subject>Cell Line</subject><subject>Cell Line, Transformed</subject><subject>Collagen - biosynthesis</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation - physiology</subject><subject>Procollagen - genetics</subject><subject>Rats</subject><subject>Receptors, Parathyroid Hormone - analysis</subject><subject>RNA, Messenger - biosynthesis</subject><subject>Skeleton and joints</subject><subject>Stem Cells - cytology</subject><subject>Stem Cells - metabolism</subject><subject>Vertebrates: osteoarticular system, musculoskeletal system</subject><issn>0884-0431</issn><issn>1523-4681</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1v1DAQhi1EVZbCmROSD4hbtv6OI06w4qNVEVKBc-TY466rrL3YiWBv_AEkfiO_hKS7anvraaSZZ955NS9CLyhZUkLY6XW3yUupJKGUaMoeoQWVjFdCafoYLYjWoiKC0yfoaSnXhBAllTpGx1pLwUSzQH--jtkbC9i4NZSQ4r_ffzfgghnA4QxXY2-GqYuTx3adosvJ7gaYoLIFG3yw-AoiYPi1zVDmfRwiHtaAY4pDNrH4lCc9fLk6x3xJrVxSjbMZ7qthC32P-xDhGTrypi_w_FBP0PcP77-tPlUXXz6erd5eVFYQwSopAZh0jSO-M7oWXhLdqcYJUnNnGG0s87wBWwPvZE2dFp1tOFOMGNoZVvMT9Hqvu83pxwhlaDehzC5MhDSWttaCMtbQB0EqaykVmcHTPWhzKiWDb7c5bEzetZS0c1LtnFR7l9S08fIgPXbTh275QzTT_NVhboo1vZ-eaUO5xThtlLrB3uyxn6GH3UNX2_N3ny_vmfgPg1iwlA</recordid><startdate>199608</startdate><enddate>199608</enddate><creator>Mcdougall, Skye</creator><creator>Fu, Y‐Hsien</creator><creator>Lowe, Gina N.</creator><creator>Williams, Alexia</creator><creator>Polendo, Ruben</creator><creator>Benya, Paul D.</creator><creator>Iida‐Klein, Akiko</creator><creator>Fang, Meika A.</creator><creator>Hahn, Theodore J.</creator><general>John Wiley and Sons and The American Society for Bone and Mineral Research (ASBMR)</general><general>American Society for Bone and Mineral Research</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7X8</scope></search><sort><creationdate>199608</creationdate><title>Surface adhesion‐mediated regulation of chondrocyte‐specific gene expression in the nontransformed RCJ 3.1c5.18 rat chondrocyte cell line</title><author>Mcdougall, Skye ; Fu, Y‐Hsien ; Lowe, Gina N. ; Williams, Alexia ; Polendo, Ruben ; Benya, Paul D. ; Iida‐Klein, Akiko ; Fang, Meika A. ; Hahn, Theodore J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4042-55ee25d9d0fba874f508b69d4073da219c2f39ec7e3b571d84bc932620a1ba273</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cartilage - cytology</topic><topic>Cartilage - metabolism</topic><topic>Cartilage - ultrastructure</topic><topic>Cell Adhesion - physiology</topic><topic>Cell Differentiation - physiology</topic><topic>Cell Line</topic><topic>Cell Line, Transformed</topic><topic>Collagen - biosynthesis</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Regulation - physiology</topic><topic>Procollagen - genetics</topic><topic>Rats</topic><topic>Receptors, Parathyroid Hormone - analysis</topic><topic>RNA, Messenger - biosynthesis</topic><topic>Skeleton and joints</topic><topic>Stem Cells - cytology</topic><topic>Stem Cells - metabolism</topic><topic>Vertebrates: osteoarticular system, musculoskeletal system</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mcdougall, Skye</creatorcontrib><creatorcontrib>Fu, Y‐Hsien</creatorcontrib><creatorcontrib>Lowe, Gina N.</creatorcontrib><creatorcontrib>Williams, Alexia</creatorcontrib><creatorcontrib>Polendo, Ruben</creatorcontrib><creatorcontrib>Benya, Paul D.</creatorcontrib><creatorcontrib>Iida‐Klein, Akiko</creatorcontrib><creatorcontrib>Fang, Meika A.</creatorcontrib><creatorcontrib>Hahn, Theodore J.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of bone and mineral research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mcdougall, Skye</au><au>Fu, Y‐Hsien</au><au>Lowe, Gina N.</au><au>Williams, Alexia</au><au>Polendo, Ruben</au><au>Benya, Paul D.</au><au>Iida‐Klein, Akiko</au><au>Fang, Meika A.</au><au>Hahn, Theodore J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Surface adhesion‐mediated regulation of chondrocyte‐specific gene expression in the nontransformed RCJ 3.1c5.18 rat chondrocyte cell line</atitle><jtitle>Journal of bone and mineral research</jtitle><addtitle>J Bone Miner Res</addtitle><date>1996-08</date><risdate>1996</risdate><volume>11</volume><issue>8</issue><spage>1130</spage><epage>1138</epage><pages>1130-1138</pages><issn>0884-0431</issn><eissn>1523-4681</eissn><coden>JBMREJ</coden><abstract>Recent evidence suggests that decreased chondrocyte function in osteoarthritis and other articular disorders may be due to chondrocyte dedifferentiation produced by altered regulatory signals from the cartilage extracellular matrix (ECM). However, there are currently no mammalian chondrocytic cell line systems adapted to the study of this process. We therefore examined the effects of ECM growth conditions on markers of differentiated chondrocytic phenotype expression in the nontransformed rat RCJ 3.1C5.18 (RCJ) chondrocyte cell line, including type II collagen expression, aggrecan production, link protein gene expression, and parathyroid hormone (PTH) receptor number. RCJ cells grown in monolayer on plastic exhibited a dedifferentiated phenotype characterized by flattened cell morphology, with >80% type I collagen and <5% type II collagen production, as determined by two‐dimensional gel mapping electrophoresis of collagen cyanogen bromide peptides. In addition, aggrecan production was low, and link protein mRNA was not expressed at detectable levels. After transfer to growth under minimal attachment conditions on the surface of a composite type I collagen/agarose (0.15%–0.8%) gel (CAG) for 7 days, RCJ cells developed a rounded, chondrocytic morphology and a pattern of differentiated, chondrocytic gene expression, with 79% type II and 8% type I collagen production. Steady‐state type I and type II procollagen mRNA levels were altered in parallel with collagen protein expression. In cells grown on CAG, aggrecan production increased 6‐fold, and there was a marked increase in both aggrecan core protein and link protein mRNA levels. In addition, maximal PTH‐stimulated cAMP generation increased 15‐fold in association with an increased PTH receptor number. Therefore, the RCJ chondrocyte cell line is highly sensitive to ECM regulation of chondrocyte‐specific gene expression.</abstract><cop>Washington, DC</cop><pub>John Wiley and Sons and The American Society for Bone and Mineral Research (ASBMR)</pub><pmid>8854249</pmid><doi>10.1002/jbmr.5650110812</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0884-0431 |
| ispartof | Journal of bone and mineral research, 1996-08, Vol.11 (8), p.1130-1138 |
| issn | 0884-0431 1523-4681 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_78412291 |
| source | MEDLINE; Oxford Academic Journals (OUP) |
| subjects | Animals Biological and medical sciences Cartilage - cytology Cartilage - metabolism Cartilage - ultrastructure Cell Adhesion - physiology Cell Differentiation - physiology Cell Line Cell Line, Transformed Collagen - biosynthesis Fundamental and applied biological sciences. Psychology Gene Expression Regulation - physiology Procollagen - genetics Rats Receptors, Parathyroid Hormone - analysis RNA, Messenger - biosynthesis Skeleton and joints Stem Cells - cytology Stem Cells - metabolism Vertebrates: osteoarticular system, musculoskeletal system |
| title | Surface adhesion‐mediated regulation of chondrocyte‐specific gene expression in the nontransformed RCJ 3.1c5.18 rat chondrocyte cell line |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-28T08%3A05%3A54IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Surface%20adhesion%E2%80%90mediated%20regulation%20of%20chondrocyte%E2%80%90specific%20gene%20expression%20in%20the%20nontransformed%20RCJ%203.1c5.18%20rat%20chondrocyte%20cell%20line&rft.jtitle=Journal%20of%20bone%20and%20mineral%20research&rft.au=Mcdougall,%20Skye&rft.date=1996-08&rft.volume=11&rft.issue=8&rft.spage=1130&rft.epage=1138&rft.pages=1130-1138&rft.issn=0884-0431&rft.eissn=1523-4681&rft.coden=JBMREJ&rft_id=info:doi/10.1002/jbmr.5650110812&rft_dat=%3Cproquest_cross%3E78412291%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=15755601&rft_id=info:pmid/8854249&rfr_iscdi=true |