Quantitation of human immunodeficiency virus type 1 RNA in cell free seminal plasma: comparison of NASBA ™ with Amplicor ™ reverse transcription-PCR amplification and correlation with quantitative culture

Human immunodeficiency virus type 1 (HIV-1) is transmitted by infected males in semen. However, the inoculum required for infection is unknown. The ability to collect such information will rely on the availability of reliable quantitative assays of HIV-1 in semen. We examined the comparative perform...

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Veröffentlicht in:	Journal of virological methods 1996-07, Vol.60 (2), p.161-170
Hauptverfasser:	Dyer, John R., Gilliam, Bruce L., Eron, Joseph J., Grosso, Lynell, Cohen, Myron S., Fiscus, Susan A.
Format:	Artikel
Sprache:	eng
Schlagworte:	AIDS/HIV Evaluation Studies as Topic HIV Infections - virology HIV-1 - genetics HIV-1 - isolation & purification HIV-1 quantitative microculture HIV-1 RNA Humans Male NASBA Nucleic Acid Amplification Techniques Polymerase Chain Reaction - methods Quantitative PCR Reproducibility of Results RNA, Viral - genetics RNA, Viral - isolation & purification Semen Semen - virology Sensitivity and Specificity Sexual transmission Viral Load - methods
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