Results of an international round robin for serum and whole-blood folate
Because of the increasing significance of folate nutriture to public health, a "round robin" interlaboratory comparison study was conducted to assess differences among methods. Twenty research laboratories participated in a 3-day analysis of six serum and six whole-blood pools. Overall mea...
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| Veröffentlicht in: | Clinical chemistry (Baltimore, Md.) Md.), 1996-10, Vol.42 (10), p.1689-1694 |
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| container_title | Clinical chemistry (Baltimore, Md.) |
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| creator | Gunter, EW Bowman, BA Caudill, SP Twite, DB Adams, MJ Sampson, EJ |
| description | Because of the increasing significance of folate nutriture to public health, a "round robin" interlaboratory comparison study was conducted to assess differences among methods. Twenty research laboratories participated in a 3-day analysis of six serum and six whole-blood pools. Overall means, SDs, and CVs derived from these results were compared within and across method types. Results reported for serum and whole-blood folate demonstrated overall CVs of 27.6% and 35.7%, respectively, across pools and two- to ninefold differences in concentrations between methods, with the greatest variation occurring at critical low folate concentrations. Although results for serum pools were less variable than those for whole-blood pools, substantial intermethod variation still occurred. The overall results underscore the urgent need for developing and validating reference methods for serum and whole-blood folate and for properly characterized reference materials. For evaluating study or clinical data, method-specific reference ranges (established with clinical confirmation of values for truly folate-deficient individuals) must be used. |
| doi_str_mv | 10.1093/clinchem/42.10.1689 |
| format | Article |
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Twenty research laboratories participated in a 3-day analysis of six serum and six whole-blood pools. Overall means, SDs, and CVs derived from these results were compared within and across method types. Results reported for serum and whole-blood folate demonstrated overall CVs of 27.6% and 35.7%, respectively, across pools and two- to ninefold differences in concentrations between methods, with the greatest variation occurring at critical low folate concentrations. Although results for serum pools were less variable than those for whole-blood pools, substantial intermethod variation still occurred. The overall results underscore the urgent need for developing and validating reference methods for serum and whole-blood folate and for properly characterized reference materials. For evaluating study or clinical data, method-specific reference ranges (established with clinical confirmation of values for truly folate-deficient individuals) must be used.</description><subject>Adult</subject><subject>Biological and medical sciences</subject><subject>Biological Assay</subject><subject>Blood Chemical Analysis - methods</subject><subject>Blood Chemical Analysis - standards</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Female</subject><subject>Folic Acid - blood</subject><subject>Humans</subject><subject>Investigative techniques, diagnostic techniques (general aspects)</subject><subject>Lactobacillus casei</subject><subject>Luminescent Measurements</subject><subject>Magnetics</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Metabolic diseases</subject><subject>Pathology. Cytology. Biochemistry. Spectrometry. 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Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</topic><topic>Quality Control</topic><topic>Radioimmunoassay</topic><topic>Reference Standards</topic><topic>Reference Values</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gunter, EW</creatorcontrib><creatorcontrib>Bowman, BA</creatorcontrib><creatorcontrib>Caudill, SP</creatorcontrib><creatorcontrib>Twite, DB</creatorcontrib><creatorcontrib>Adams, MJ</creatorcontrib><creatorcontrib>Sampson, EJ</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Clinical chemistry (Baltimore, Md.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gunter, EW</au><au>Bowman, BA</au><au>Caudill, SP</au><au>Twite, DB</au><au>Adams, MJ</au><au>Sampson, EJ</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Results of an international round robin for serum and whole-blood folate</atitle><jtitle>Clinical chemistry (Baltimore, Md.)</jtitle><addtitle>Clin Chem</addtitle><date>1996-10-01</date><risdate>1996</risdate><volume>42</volume><issue>10</issue><spage>1689</spage><epage>1694</epage><pages>1689-1694</pages><issn>0009-9147</issn><eissn>1530-8561</eissn><coden>CLCHAU</coden><abstract>Because of the increasing significance of folate nutriture to public health, a "round robin" interlaboratory comparison study was conducted to assess differences among methods. Twenty research laboratories participated in a 3-day analysis of six serum and six whole-blood pools. Overall means, SDs, and CVs derived from these results were compared within and across method types. Results reported for serum and whole-blood folate demonstrated overall CVs of 27.6% and 35.7%, respectively, across pools and two- to ninefold differences in concentrations between methods, with the greatest variation occurring at critical low folate concentrations. Although results for serum pools were less variable than those for whole-blood pools, substantial intermethod variation still occurred. The overall results underscore the urgent need for developing and validating reference methods for serum and whole-blood folate and for properly characterized reference materials. 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| ispartof | Clinical chemistry (Baltimore, Md.), 1996-10, Vol.42 (10), p.1689-1694 |
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| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE |
| subjects | Adult Biological and medical sciences Biological Assay Blood Chemical Analysis - methods Blood Chemical Analysis - standards Chromatography, High Pressure Liquid Female Folic Acid - blood Humans Investigative techniques, diagnostic techniques (general aspects) Lactobacillus casei Luminescent Measurements Magnetics Male Medical sciences Metabolic diseases Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques Quality Control Radioimmunoassay Reference Standards Reference Values |
| title | Results of an international round robin for serum and whole-blood folate |
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