Neutrophil lactoferrin content: Variation among mammals
Lactoferrin (Lf) in blood and/or marrow neutrophils was semiquantified using indirect immunofluorescence technique in nine mammalian species. Neutrophil iron‐binding reactivity (NFeBR), which corresponds primarily to Lf, was also visualized and semiquantified using functional cytochemical (FeNTA‐AF)...
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| Veröffentlicht in: | The Anatomical record 1988-06, Vol.221 (2), p.567-575 |
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| container_title | The Anatomical record |
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| creator | Barton, James C. Parmley, Richard T. Butler, Thomas W. Williamson, Sue Mackenzie, Sandra Chandler, David B. Blackburn, Warren Heck, Louis W. |
| description | Lactoferrin (Lf) in blood and/or marrow neutrophils was semiquantified using indirect immunofluorescence technique in nine mammalian species. Neutrophil iron‐binding reactivity (NFeBR), which corresponds primarily to Lf, was also visualized and semiquantified using functional cytochemical (FeNTA‐AF) technique at the light microscopic level in these nine and in an additional fifteen mammalian species, and in selected species at the ultrastructural level. Neutrophil immunoreactive Lf was positively correlated with total cellular and granule content of NFeBR among these nine species, and with previously reported concentrations of neutrophil Lf quantified by radioimmunoassay. Relative levels of Lf in neutrophil extracts from rat, hamster, and human were confirmed using SDS‐polyacrylamide gel electrophoresis and immunoblotting. Relatively high levels of immunoreactive neutrophil Lf and/or NFeBR were observed in carnivores (ten species) and primates (six species). Among rodents (five species), the levels were variable, and the artiodactyls (four species) studied had low levels. These results demonstrate that neutrophil Lf levels vary widely among mammalian species. In addition, FeNTA‐AF technique provides a rapid means of evaluating animals for relative quantities of neutrophil Lf. |
| doi_str_mv | 10.1002/ar.1092210202 |
| format | Article |
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Neutrophil iron‐binding reactivity (NFeBR), which corresponds primarily to Lf, was also visualized and semiquantified using functional cytochemical (FeNTA‐AF) technique at the light microscopic level in these nine and in an additional fifteen mammalian species, and in selected species at the ultrastructural level. Neutrophil immunoreactive Lf was positively correlated with total cellular and granule content of NFeBR among these nine species, and with previously reported concentrations of neutrophil Lf quantified by radioimmunoassay. Relative levels of Lf in neutrophil extracts from rat, hamster, and human were confirmed using SDS‐polyacrylamide gel electrophoresis and immunoblotting. Relatively high levels of immunoreactive neutrophil Lf and/or NFeBR were observed in carnivores (ten species) and primates (six species). Among rodents (five species), the levels were variable, and the artiodactyls (four species) studied had low levels. These results demonstrate that neutrophil Lf levels vary widely among mammalian species. In addition, FeNTA‐AF technique provides a rapid means of evaluating animals for relative quantities of neutrophil Lf.</description><identifier>ISSN: 0003-276X</identifier><identifier>EISSN: 1097-0185</identifier><identifier>DOI: 10.1002/ar.1092210202</identifier><identifier>PMID: 3046435</identifier><identifier>CODEN: ANREAK</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Analytical, structural and metabolic biochemistry ; Animals ; Artiodactyla - metabolism ; Biological and medical sciences ; Bone Marrow - analysis ; Carnivora - metabolism ; Fluorescent Antibody Technique ; Fundamental and applied biological sciences. Psychology ; Humans ; Immunoassay ; Lactoferrin - analysis ; Lactoglobulins - analysis ; Mammals - metabolism ; Metalloproteins ; Neutrophils - analysis ; Neutrophils - ultrastructure ; Other metalloproteins ; Primates - metabolism ; Proteins ; Rodentia - metabolism ; Species Specificity</subject><ispartof>The Anatomical record, 1988-06, Vol.221 (2), p.567-575</ispartof><rights>Copyright © 1988 Wiley‐Liss, Inc.</rights><rights>1989 INIST-CNRS</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4312-1afc841d6fce041a66ed8f1fcf24bf199ec8b0617497101a022527657391d60f3</citedby><cites>FETCH-LOGICAL-c4312-1afc841d6fce041a66ed8f1fcf24bf199ec8b0617497101a022527657391d60f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Far.1092210202$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Far.1092210202$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7064083$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3046435$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Barton, James C.</creatorcontrib><creatorcontrib>Parmley, Richard T.</creatorcontrib><creatorcontrib>Butler, Thomas W.</creatorcontrib><creatorcontrib>Williamson, Sue</creatorcontrib><creatorcontrib>Mackenzie, Sandra</creatorcontrib><creatorcontrib>Chandler, David B.</creatorcontrib><creatorcontrib>Blackburn, Warren</creatorcontrib><creatorcontrib>Heck, Louis W.</creatorcontrib><title>Neutrophil lactoferrin content: Variation among mammals</title><title>The Anatomical record</title><addtitle>Anat Rec</addtitle><description>Lactoferrin (Lf) in blood and/or marrow neutrophils was semiquantified using indirect immunofluorescence technique in nine mammalian species. Neutrophil iron‐binding reactivity (NFeBR), which corresponds primarily to Lf, was also visualized and semiquantified using functional cytochemical (FeNTA‐AF) technique at the light microscopic level in these nine and in an additional fifteen mammalian species, and in selected species at the ultrastructural level. Neutrophil immunoreactive Lf was positively correlated with total cellular and granule content of NFeBR among these nine species, and with previously reported concentrations of neutrophil Lf quantified by radioimmunoassay. Relative levels of Lf in neutrophil extracts from rat, hamster, and human were confirmed using SDS‐polyacrylamide gel electrophoresis and immunoblotting. Relatively high levels of immunoreactive neutrophil Lf and/or NFeBR were observed in carnivores (ten species) and primates (six species). Among rodents (five species), the levels were variable, and the artiodactyls (four species) studied had low levels. These results demonstrate that neutrophil Lf levels vary widely among mammalian species. In addition, FeNTA‐AF technique provides a rapid means of evaluating animals for relative quantities of neutrophil Lf.</description><subject>Analytical, structural and metabolic biochemistry</subject><subject>Animals</subject><subject>Artiodactyla - metabolism</subject><subject>Biological and medical sciences</subject><subject>Bone Marrow - analysis</subject><subject>Carnivora - metabolism</subject><subject>Fluorescent Antibody Technique</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Immunoassay</subject><subject>Lactoferrin - analysis</subject><subject>Lactoglobulins - analysis</subject><subject>Mammals - metabolism</subject><subject>Metalloproteins</subject><subject>Neutrophils - analysis</subject><subject>Neutrophils - ultrastructure</subject><subject>Other metalloproteins</subject><subject>Primates - metabolism</subject><subject>Proteins</subject><subject>Rodentia - metabolism</subject><subject>Species Specificity</subject><issn>0003-276X</issn><issn>1097-0185</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1988</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kM1LxDAQxYMo67p69Cj0IN6qM0matN6WxS9YFETFW8lmE620zZq0yP73Rrasnjw9hvnNm8cj5BjhHAHohfJRC0oRKNAdMo6DTAHzbJeMAYClVIrXfXIQwgcAIhdiREYMuOAsGxN5b_rOu9V7VSe10p2zxvuqTbRrO9N2l8mL8pXqKtcmqnHtW9KoplF1OCR7Noo5GnRCnq-vnma36fzh5m42naeaM6QpKqtzjkthtQGOSgizzC1abSlfWCwKo_MFCJS8kAiogNIs5s0kK-IRWDYhZxvflXefvQld2VRBm7pWrXF9KGXOiqIAjGC6AbV3IXhjy5WvGuXXJUL5U1SpfPlbVORPBuN-0Zjllh6aifvTYa-CVrX1qtVV2GISBIecRUxusK-qNuv_f5bTxz8BvgFYtn6m</recordid><startdate>198806</startdate><enddate>198806</enddate><creator>Barton, James C.</creator><creator>Parmley, Richard T.</creator><creator>Butler, Thomas W.</creator><creator>Williamson, Sue</creator><creator>Mackenzie, Sandra</creator><creator>Chandler, David B.</creator><creator>Blackburn, Warren</creator><creator>Heck, Louis W.</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley-Liss</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>198806</creationdate><title>Neutrophil lactoferrin content: Variation among mammals</title><author>Barton, James C. ; Parmley, Richard T. ; Butler, Thomas W. ; Williamson, Sue ; Mackenzie, Sandra ; Chandler, David B. ; Blackburn, Warren ; Heck, Louis W.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4312-1afc841d6fce041a66ed8f1fcf24bf199ec8b0617497101a022527657391d60f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1988</creationdate><topic>Analytical, structural and metabolic biochemistry</topic><topic>Animals</topic><topic>Artiodactyla - metabolism</topic><topic>Biological and medical sciences</topic><topic>Bone Marrow - analysis</topic><topic>Carnivora - metabolism</topic><topic>Fluorescent Antibody Technique</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Immunoassay</topic><topic>Lactoferrin - analysis</topic><topic>Lactoglobulins - analysis</topic><topic>Mammals - metabolism</topic><topic>Metalloproteins</topic><topic>Neutrophils - analysis</topic><topic>Neutrophils - ultrastructure</topic><topic>Other metalloproteins</topic><topic>Primates - metabolism</topic><topic>Proteins</topic><topic>Rodentia - metabolism</topic><topic>Species Specificity</topic><toplevel>online_resources</toplevel><creatorcontrib>Barton, James C.</creatorcontrib><creatorcontrib>Parmley, Richard T.</creatorcontrib><creatorcontrib>Butler, Thomas W.</creatorcontrib><creatorcontrib>Williamson, Sue</creatorcontrib><creatorcontrib>Mackenzie, Sandra</creatorcontrib><creatorcontrib>Chandler, David B.</creatorcontrib><creatorcontrib>Blackburn, Warren</creatorcontrib><creatorcontrib>Heck, Louis W.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Anatomical record</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Barton, James C.</au><au>Parmley, Richard T.</au><au>Butler, Thomas W.</au><au>Williamson, Sue</au><au>Mackenzie, Sandra</au><au>Chandler, David B.</au><au>Blackburn, Warren</au><au>Heck, Louis W.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Neutrophil lactoferrin content: Variation among mammals</atitle><jtitle>The Anatomical record</jtitle><addtitle>Anat Rec</addtitle><date>1988-06</date><risdate>1988</risdate><volume>221</volume><issue>2</issue><spage>567</spage><epage>575</epage><pages>567-575</pages><issn>0003-276X</issn><eissn>1097-0185</eissn><coden>ANREAK</coden><abstract>Lactoferrin (Lf) in blood and/or marrow neutrophils was semiquantified using indirect immunofluorescence technique in nine mammalian species. Neutrophil iron‐binding reactivity (NFeBR), which corresponds primarily to Lf, was also visualized and semiquantified using functional cytochemical (FeNTA‐AF) technique at the light microscopic level in these nine and in an additional fifteen mammalian species, and in selected species at the ultrastructural level. Neutrophil immunoreactive Lf was positively correlated with total cellular and granule content of NFeBR among these nine species, and with previously reported concentrations of neutrophil Lf quantified by radioimmunoassay. Relative levels of Lf in neutrophil extracts from rat, hamster, and human were confirmed using SDS‐polyacrylamide gel electrophoresis and immunoblotting. Relatively high levels of immunoreactive neutrophil Lf and/or NFeBR were observed in carnivores (ten species) and primates (six species). Among rodents (five species), the levels were variable, and the artiodactyls (four species) studied had low levels. These results demonstrate that neutrophil Lf levels vary widely among mammalian species. In addition, FeNTA‐AF technique provides a rapid means of evaluating animals for relative quantities of neutrophil Lf.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>3046435</pmid><doi>10.1002/ar.1092210202</doi><tpages>9</tpages></addata></record> |
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| subjects | Analytical, structural and metabolic biochemistry Animals Artiodactyla - metabolism Biological and medical sciences Bone Marrow - analysis Carnivora - metabolism Fluorescent Antibody Technique Fundamental and applied biological sciences. Psychology Humans Immunoassay Lactoferrin - analysis Lactoglobulins - analysis Mammals - metabolism Metalloproteins Neutrophils - analysis Neutrophils - ultrastructure Other metalloproteins Primates - metabolism Proteins Rodentia - metabolism Species Specificity |
| title | Neutrophil lactoferrin content: Variation among mammals |
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