Myoplasmic [Ca2+] Determines Myosin Phosphorylation in Agonist-Stimulated Swine Arterial Smooth Muscle
Our objective was to test the hypotheses that 1) myoplasmic [Ca] is the primary determinant of crossbridge phosphorylation and that 2) phosphorylation is the primary determinant of crossbridge interactions with the thin filament in swine carotid arterial smooth muscle. We tested these hypotheses by...
Gespeichert in:
| Veröffentlicht in: | Circulation research 1988-09, Vol.63 (3), p.593-603 |
|---|---|
| Hauptverfasser: | , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 603 |
|---|---|
| container_issue | 3 |
| container_start_page | 593 |
| container_title | Circulation research |
| container_volume | 63 |
| creator | Rembold, Christopher M Murphy, Richard A |
| description | Our objective was to test the hypotheses that 1) myoplasmic [Ca] is the primary determinant of crossbridge phosphorylation and that 2) phosphorylation is the primary determinant of crossbridge interactions with the thin filament in swine carotid arterial smooth muscle. We tested these hypotheses by evaluating the relation between aequorin-estimated myoplasmic [Ca], myosin light chain phosphorylation, shortening velocity at zero load (Vo), and stress at various times after stimulation with histamine, phenylephrine, and depolarization with KC1. Agonist-induced changes in myoplasmic [Ca] were associated with predictable changes in myosin phosphorylation. Depolarization required proportionally higher changes in myoplasmic[Ca] for a given change In myosin phosphorylation. The relation between phosphorylation and Vo or steady-state stress was invariant with all tested stimuli. This suggests that Ca-dependent crossbridge phosphorylation is the primary determinant of the mechanical response. |
| doi_str_mv | 10.1161/01.res.63.3.593 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_78396788</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>78396788</sourcerecordid><originalsourceid>FETCH-LOGICAL-c5861-8bf0adf7ca6917b0f6b663e99e5fba5a1f7d62bcf233c254e4438a1419f51c0d3</originalsourceid><addsrcrecordid>eNqFkUtv1DAUhS0EKkPLmhVSFqgblNQ3fiRejoY-kFqBmLJCyHIcmxiceLATjebf42pG3bKwrnzPd87iHoTeAa4AOFxhqKJJFScVqZggL9AKWE1Lyhp4iVYYY1E2hODX6E1KvzEGSmpxhs4IxYIKvEL24RB2XqXR6eLHRtUffxafzGzi6CaTiiwmNxVfh5B2Q4gHr2YXpiKv1r_C5NJcbmc3Lnlt-mK7z55iHbPbKV9sxxDmoXhYkvbmAr2yyifz9jTP0feb68fNXXn_5fbzZn1fatZyKNvOYtXbRisuoOmw5R3nxAhhmO0UU2CbntedtjUhumbUUEpaBRSEZaBxT87R5TF3F8PfxaRZji5p472aTFiSbFoieNO2_wWBYd42AjJ4dQR1DClFY-UuulHFgwQsnyqQGOS3663kRBKZK8iO96fopRtN_8yfbp71DyddJa28jWrSLj1jXAhgzVMMPWL74PNJ0x-_7E2Ug1F-HmRuFhMMdQmibbHIvzI_APIPS8mfLA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>15068791</pqid></control><display><type>article</type><title>Myoplasmic [Ca2+] Determines Myosin Phosphorylation in Agonist-Stimulated Swine Arterial Smooth Muscle</title><source>MEDLINE</source><source>American Heart Association Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Journals@Ovid Complete</source><creator>Rembold, Christopher M ; Murphy, Richard A</creator><creatorcontrib>Rembold, Christopher M ; Murphy, Richard A</creatorcontrib><description>Our objective was to test the hypotheses that 1) myoplasmic [Ca] is the primary determinant of crossbridge phosphorylation and that 2) phosphorylation is the primary determinant of crossbridge interactions with the thin filament in swine carotid arterial smooth muscle. We tested these hypotheses by evaluating the relation between aequorin-estimated myoplasmic [Ca], myosin light chain phosphorylation, shortening velocity at zero load (Vo), and stress at various times after stimulation with histamine, phenylephrine, and depolarization with KC1. Agonist-induced changes in myoplasmic [Ca] were associated with predictable changes in myosin phosphorylation. Depolarization required proportionally higher changes in myoplasmic[Ca] for a given change In myosin phosphorylation. The relation between phosphorylation and Vo or steady-state stress was invariant with all tested stimuli. This suggests that Ca-dependent crossbridge phosphorylation is the primary determinant of the mechanical response.</description><identifier>ISSN: 0009-7330</identifier><identifier>EISSN: 1524-4571</identifier><identifier>DOI: 10.1161/01.res.63.3.593</identifier><identifier>PMID: 3409490</identifier><identifier>CODEN: CIRUAL</identifier><language>eng</language><publisher>Hagerstown, MD: American Heart Association, Inc</publisher><subject>Aequorin - pharmacology ; Animals ; Biological and medical sciences ; Blood vessels and receptors ; Calcium - metabolism ; Calibration ; Carotid Arteries ; Fundamental and applied biological sciences. Psychology ; In Vitro Techniques ; Muscle, Smooth, Vascular - metabolism ; Muscle, Smooth, Vascular - ultrastructure ; Myosins - metabolism ; Osmolar Concentration ; Phosphorylation ; Photometry ; Stimulation, Chemical ; Swine ; Vasoconstriction ; Vertebrates: cardiovascular system</subject><ispartof>Circulation research, 1988-09, Vol.63 (3), p.593-603</ispartof><rights>1988 American Heart Association, Inc.</rights><rights>1989 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5861-8bf0adf7ca6917b0f6b663e99e5fba5a1f7d62bcf233c254e4438a1419f51c0d3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,3687,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=6991573$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3409490$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rembold, Christopher M</creatorcontrib><creatorcontrib>Murphy, Richard A</creatorcontrib><title>Myoplasmic [Ca2+] Determines Myosin Phosphorylation in Agonist-Stimulated Swine Arterial Smooth Muscle</title><title>Circulation research</title><addtitle>Circ Res</addtitle><description>Our objective was to test the hypotheses that 1) myoplasmic [Ca] is the primary determinant of crossbridge phosphorylation and that 2) phosphorylation is the primary determinant of crossbridge interactions with the thin filament in swine carotid arterial smooth muscle. We tested these hypotheses by evaluating the relation between aequorin-estimated myoplasmic [Ca], myosin light chain phosphorylation, shortening velocity at zero load (Vo), and stress at various times after stimulation with histamine, phenylephrine, and depolarization with KC1. Agonist-induced changes in myoplasmic [Ca] were associated with predictable changes in myosin phosphorylation. Depolarization required proportionally higher changes in myoplasmic[Ca] for a given change In myosin phosphorylation. The relation between phosphorylation and Vo or steady-state stress was invariant with all tested stimuli. This suggests that Ca-dependent crossbridge phosphorylation is the primary determinant of the mechanical response.</description><subject>Aequorin - pharmacology</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Blood vessels and receptors</subject><subject>Calcium - metabolism</subject><subject>Calibration</subject><subject>Carotid Arteries</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>In Vitro Techniques</subject><subject>Muscle, Smooth, Vascular - metabolism</subject><subject>Muscle, Smooth, Vascular - ultrastructure</subject><subject>Myosins - metabolism</subject><subject>Osmolar Concentration</subject><subject>Phosphorylation</subject><subject>Photometry</subject><subject>Stimulation, Chemical</subject><subject>Swine</subject><subject>Vasoconstriction</subject><subject>Vertebrates: cardiovascular system</subject><issn>0009-7330</issn><issn>1524-4571</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1988</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtv1DAUhS0EKkPLmhVSFqgblNQ3fiRejoY-kFqBmLJCyHIcmxiceLATjebf42pG3bKwrnzPd87iHoTeAa4AOFxhqKJJFScVqZggL9AKWE1Lyhp4iVYYY1E2hODX6E1KvzEGSmpxhs4IxYIKvEL24RB2XqXR6eLHRtUffxafzGzi6CaTiiwmNxVfh5B2Q4gHr2YXpiKv1r_C5NJcbmc3Lnlt-mK7z55iHbPbKV9sxxDmoXhYkvbmAr2yyifz9jTP0feb68fNXXn_5fbzZn1fatZyKNvOYtXbRisuoOmw5R3nxAhhmO0UU2CbntedtjUhumbUUEpaBRSEZaBxT87R5TF3F8PfxaRZji5p472aTFiSbFoieNO2_wWBYd42AjJ4dQR1DClFY-UuulHFgwQsnyqQGOS3663kRBKZK8iO96fopRtN_8yfbp71DyddJa28jWrSLj1jXAhgzVMMPWL74PNJ0x-_7E2Ug1F-HmRuFhMMdQmibbHIvzI_APIPS8mfLA</recordid><startdate>198809</startdate><enddate>198809</enddate><creator>Rembold, Christopher M</creator><creator>Murphy, Richard A</creator><general>American Heart Association, Inc</general><general>Lippincott</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7X8</scope></search><sort><creationdate>198809</creationdate><title>Myoplasmic [Ca2+] Determines Myosin Phosphorylation in Agonist-Stimulated Swine Arterial Smooth Muscle</title><author>Rembold, Christopher M ; Murphy, Richard A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5861-8bf0adf7ca6917b0f6b663e99e5fba5a1f7d62bcf233c254e4438a1419f51c0d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1988</creationdate><topic>Aequorin - pharmacology</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Blood vessels and receptors</topic><topic>Calcium - metabolism</topic><topic>Calibration</topic><topic>Carotid Arteries</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>In Vitro Techniques</topic><topic>Muscle, Smooth, Vascular - metabolism</topic><topic>Muscle, Smooth, Vascular - ultrastructure</topic><topic>Myosins - metabolism</topic><topic>Osmolar Concentration</topic><topic>Phosphorylation</topic><topic>Photometry</topic><topic>Stimulation, Chemical</topic><topic>Swine</topic><topic>Vasoconstriction</topic><topic>Vertebrates: cardiovascular system</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rembold, Christopher M</creatorcontrib><creatorcontrib>Murphy, Richard A</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Circulation research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rembold, Christopher M</au><au>Murphy, Richard A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Myoplasmic [Ca2+] Determines Myosin Phosphorylation in Agonist-Stimulated Swine Arterial Smooth Muscle</atitle><jtitle>Circulation research</jtitle><addtitle>Circ Res</addtitle><date>1988-09</date><risdate>1988</risdate><volume>63</volume><issue>3</issue><spage>593</spage><epage>603</epage><pages>593-603</pages><issn>0009-7330</issn><eissn>1524-4571</eissn><coden>CIRUAL</coden><abstract>Our objective was to test the hypotheses that 1) myoplasmic [Ca] is the primary determinant of crossbridge phosphorylation and that 2) phosphorylation is the primary determinant of crossbridge interactions with the thin filament in swine carotid arterial smooth muscle. We tested these hypotheses by evaluating the relation between aequorin-estimated myoplasmic [Ca], myosin light chain phosphorylation, shortening velocity at zero load (Vo), and stress at various times after stimulation with histamine, phenylephrine, and depolarization with KC1. Agonist-induced changes in myoplasmic [Ca] were associated with predictable changes in myosin phosphorylation. Depolarization required proportionally higher changes in myoplasmic[Ca] for a given change In myosin phosphorylation. The relation between phosphorylation and Vo or steady-state stress was invariant with all tested stimuli. This suggests that Ca-dependent crossbridge phosphorylation is the primary determinant of the mechanical response.</abstract><cop>Hagerstown, MD</cop><pub>American Heart Association, Inc</pub><pmid>3409490</pmid><doi>10.1161/01.res.63.3.593</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0009-7330 |
| ispartof | Circulation research, 1988-09, Vol.63 (3), p.593-603 |
| issn | 0009-7330 1524-4571 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_78396788 |
| source | MEDLINE; American Heart Association Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Journals@Ovid Complete |
| subjects | Aequorin - pharmacology Animals Biological and medical sciences Blood vessels and receptors Calcium - metabolism Calibration Carotid Arteries Fundamental and applied biological sciences. Psychology In Vitro Techniques Muscle, Smooth, Vascular - metabolism Muscle, Smooth, Vascular - ultrastructure Myosins - metabolism Osmolar Concentration Phosphorylation Photometry Stimulation, Chemical Swine Vasoconstriction Vertebrates: cardiovascular system |
| title | Myoplasmic [Ca2+] Determines Myosin Phosphorylation in Agonist-Stimulated Swine Arterial Smooth Muscle |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-21T10%3A18%3A47IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Myoplasmic%20%5BCa2+%5D%20Determines%20Myosin%20Phosphorylation%20in%20Agonist-Stimulated%20Swine%20Arterial%20Smooth%20Muscle&rft.jtitle=Circulation%20research&rft.au=Rembold,%20Christopher%20M&rft.date=1988-09&rft.volume=63&rft.issue=3&rft.spage=593&rft.epage=603&rft.pages=593-603&rft.issn=0009-7330&rft.eissn=1524-4571&rft.coden=CIRUAL&rft_id=info:doi/10.1161/01.res.63.3.593&rft_dat=%3Cproquest_cross%3E78396788%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=15068791&rft_id=info:pmid/3409490&rfr_iscdi=true |