Molecular cloning and mapping of rat cytomegalovirus DNA
The DNA of a rat cytomegalovirus (RCMV) isolate from England is cleaved by HindIII into 25 fragments, 20 of which have been cloned into recombinant plasmids. Twenty-two of thirty KpnI fragments were similarly cloned which, together with the HindIII clones, resulted in cloning of 93% of the viral gen...
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| Veröffentlicht in: | Virology (New York, N.Y.) N.Y.), 1988-09, Vol.166 (1), p.140-148 |
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| container_title | Virology (New York, N.Y.) |
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| creator | Burns, William H. Barbour, Gene M. Sandford, Gordon R. |
| description | The DNA of a rat cytomegalovirus (RCMV) isolate from England is cleaved by
HindIII into 25 fragments, 20 of which have been cloned into recombinant plasmids. Twenty-two of thirty
KpnI fragments were similarly cloned which, together with the
HindIII clones, resulted in cloning of 93% of the viral genome. Terminal fragments were identified and restriction site maps were constructed of the viral genome for
HindIII,
KpnI, and
HpaI. The lack of cross-hybridization among fragments derived with each restriction enzyme and the finding of only two termini support the view that RCMV DNA consists of one long unique sequence. The size of the genome of the English strain of RCMV is approximately 206 kbp, much smaller than that of the previously reported “Dutch” strain. |
| doi_str_mv | 10.1016/0042-6822(88)90155-9 |
| format | Article |
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HindIII into 25 fragments, 20 of which have been cloned into recombinant plasmids. Twenty-two of thirty
KpnI fragments were similarly cloned which, together with the
HindIII clones, resulted in cloning of 93% of the viral genome. Terminal fragments were identified and restriction site maps were constructed of the viral genome for
HindIII,
KpnI, and
HpaI. The lack of cross-hybridization among fragments derived with each restriction enzyme and the finding of only two termini support the view that RCMV DNA consists of one long unique sequence. The size of the genome of the English strain of RCMV is approximately 206 kbp, much smaller than that of the previously reported “Dutch” strain.</description><identifier>ISSN: 0042-6822</identifier><identifier>EISSN: 1096-0341</identifier><identifier>DOI: 10.1016/0042-6822(88)90155-9</identifier><identifier>PMID: 2842940</identifier><identifier>CODEN: VIRLAX</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>Animals ; Biological and medical sciences ; Chromosome Mapping ; Cloning, Molecular ; Cytomegalovirus - genetics ; DNA Restriction Enzymes ; DNA, Viral - genetics ; Fundamental and applied biological sciences. Psychology ; Genetics ; Microbiology ; Rats ; Virology</subject><ispartof>Virology (New York, N.Y.), 1988-09, Vol.166 (1), p.140-148</ispartof><rights>1988</rights><rights>1989 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3789-b1da36d1f26a1b61f4a3b800c908fbd55c143fa6f987035eb5d7334b6b3b04153</citedby><cites>FETCH-LOGICAL-c3789-b1da36d1f26a1b61f4a3b800c908fbd55c143fa6f987035eb5d7334b6b3b04153</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0042682288901559$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7206366$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2842940$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Burns, William H.</creatorcontrib><creatorcontrib>Barbour, Gene M.</creatorcontrib><creatorcontrib>Sandford, Gordon R.</creatorcontrib><title>Molecular cloning and mapping of rat cytomegalovirus DNA</title><title>Virology (New York, N.Y.)</title><addtitle>Virology</addtitle><description>The DNA of a rat cytomegalovirus (RCMV) isolate from England is cleaved by
HindIII into 25 fragments, 20 of which have been cloned into recombinant plasmids. Twenty-two of thirty
KpnI fragments were similarly cloned which, together with the
HindIII clones, resulted in cloning of 93% of the viral genome. Terminal fragments were identified and restriction site maps were constructed of the viral genome for
HindIII,
KpnI, and
HpaI. The lack of cross-hybridization among fragments derived with each restriction enzyme and the finding of only two termini support the view that RCMV DNA consists of one long unique sequence. The size of the genome of the English strain of RCMV is approximately 206 kbp, much smaller than that of the previously reported “Dutch” strain.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Chromosome Mapping</subject><subject>Cloning, Molecular</subject><subject>Cytomegalovirus - genetics</subject><subject>DNA Restriction Enzymes</subject><subject>DNA, Viral - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetics</subject><subject>Microbiology</subject><subject>Rats</subject><subject>Virology</subject><issn>0042-6822</issn><issn>1096-0341</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1988</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1r3DAQhkVo2W7S_IMEfCghObidsWRZugRCPtpCPi7JWUiytKjY1layA_n38WaXPbanmeF9ZhgeQk4QviMg_wHAqpKLqjoX4kIC1nUpD8gSQfISKMNPZLlHvpDDnP_APDcNLMiiEqySDJZEPMTO2anTqbBdHMKwKvTQFr1erzd99EXSY2Hfxti7le7ia0hTLm4er76Sz1532R3v6hF5ubt9vv5V3j_9_H19dV9a2ghZGmw15S36ims0HD3T1AgAK0F409a1RUa95l6KBmjtTN02lDLDDTXAsKZH5Gx7d53i38nlUfUhW9d1enBxyqoRVDKk4r8g1sgYSjqDbAvaFHNOzqt1Cr1ObwpBbcyqjTa10aaEUB9mlZzXTnf3J9O7dr-0Uznn33a5zlZ3PunBhrzHmgo45XzGLreYm6W9BpdUtsEN1rUhOTuqNoZ___EOFWySZw</recordid><startdate>198809</startdate><enddate>198809</enddate><creator>Burns, William H.</creator><creator>Barbour, Gene M.</creator><creator>Sandford, Gordon R.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>198809</creationdate><title>Molecular cloning and mapping of rat cytomegalovirus DNA</title><author>Burns, William H. ; Barbour, Gene M. ; Sandford, Gordon R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3789-b1da36d1f26a1b61f4a3b800c908fbd55c143fa6f987035eb5d7334b6b3b04153</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1988</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Chromosome Mapping</topic><topic>Cloning, Molecular</topic><topic>Cytomegalovirus - genetics</topic><topic>DNA Restriction Enzymes</topic><topic>DNA, Viral - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetics</topic><topic>Microbiology</topic><topic>Rats</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Burns, William H.</creatorcontrib><creatorcontrib>Barbour, Gene M.</creatorcontrib><creatorcontrib>Sandford, Gordon R.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Virology (New York, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Burns, William H.</au><au>Barbour, Gene M.</au><au>Sandford, Gordon R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular cloning and mapping of rat cytomegalovirus DNA</atitle><jtitle>Virology (New York, N.Y.)</jtitle><addtitle>Virology</addtitle><date>1988-09</date><risdate>1988</risdate><volume>166</volume><issue>1</issue><spage>140</spage><epage>148</epage><pages>140-148</pages><issn>0042-6822</issn><eissn>1096-0341</eissn><coden>VIRLAX</coden><abstract>The DNA of a rat cytomegalovirus (RCMV) isolate from England is cleaved by
HindIII into 25 fragments, 20 of which have been cloned into recombinant plasmids. Twenty-two of thirty
KpnI fragments were similarly cloned which, together with the
HindIII clones, resulted in cloning of 93% of the viral genome. Terminal fragments were identified and restriction site maps were constructed of the viral genome for
HindIII,
KpnI, and
HpaI. The lack of cross-hybridization among fragments derived with each restriction enzyme and the finding of only two termini support the view that RCMV DNA consists of one long unique sequence. The size of the genome of the English strain of RCMV is approximately 206 kbp, much smaller than that of the previously reported “Dutch” strain.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>2842940</pmid><doi>10.1016/0042-6822(88)90155-9</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0042-6822 |
| ispartof | Virology (New York, N.Y.), 1988-09, Vol.166 (1), p.140-148 |
| issn | 0042-6822 1096-0341 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Animals Biological and medical sciences Chromosome Mapping Cloning, Molecular Cytomegalovirus - genetics DNA Restriction Enzymes DNA, Viral - genetics Fundamental and applied biological sciences. Psychology Genetics Microbiology Rats Virology |
| title | Molecular cloning and mapping of rat cytomegalovirus DNA |
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