A source of error in phenylketonuria screening
The escalating number of blood specimens from late-fed premature or very sick newborns greatly increases the risk of missing the diagnosis of phenylketonuria (PKU). Babies receiving antibiotics have uninterpretable "clear-zone" results with the traditional Guthrie bacteriologic inhibition...
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| Veröffentlicht in: | American journal of clinical pathology 1988-09, Vol.90 (3), p.279-283 |
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| container_title | American journal of clinical pathology |
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| creator | MABRY, C. C REID, M. C KUHN, R. J |
| description | The escalating number of blood specimens from late-fed premature or very sick newborns greatly increases the risk of missing the diagnosis of phenylketonuria (PKU). Babies receiving antibiotics have uninterpretable "clear-zone" results with the traditional Guthrie bacteriologic inhibition assay (BIA). For the past year the authors have reexamined the blood phenylalanine level on specimens giving the "clear-zone" effect by BIA by use of the McCaman-Robins chemical-fluorescent assay (CFA). Spuriously high blood phenylalanine levels occurred in four babies who were receiving ampicillin and whose specimens were collected on filter paper and autoclaved in preparation for the BIA. None of the babies proved to have PKU. The fluorescent interference caused by ampicillin resulted from the heat of autoclaving the specimen. The authors recommend that the blood specimen should not be autoclaved before analysis by either BIA or CFA. |
| doi_str_mv | 10.1093/ajcp/90.3.279 |
| format | Article |
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The authors recommend that the blood specimen should not be autoclaved before analysis by either BIA or CFA.</description><identifier>ISSN: 0002-9173</identifier><identifier>EISSN: 1943-7722</identifier><identifier>DOI: 10.1093/ajcp/90.3.279</identifier><identifier>PMID: 3137803</identifier><identifier>CODEN: AJCPAI</identifier><language>eng</language><publisher>Chicago, IL: American Society of Clinical Pathologists</publisher><subject>Agar ; Aminoacid disorders ; Ampicillin - pharmacology ; Ampicillin - therapeutic use ; Bacillus subtilis - growth & development ; Biological and medical sciences ; Diffusion ; Errors of metabolism ; False Positive Reactions ; Female ; Fluorescence ; Hot Temperature ; Humans ; Infant, Newborn ; Infant, Premature ; Male ; Medical sciences ; Metabolic diseases ; Phenylalanine - blood ; Phenylketonurias - blood</subject><ispartof>American journal of clinical pathology, 1988-09, Vol.90 (3), p.279-283</ispartof><rights>1989 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c317t-fa14d75571899648208eb68e51572e28a426b48c2b333dde9fa553ae4c0b966d3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7176453$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3137803$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>MABRY, C. C</creatorcontrib><creatorcontrib>REID, M. C</creatorcontrib><creatorcontrib>KUHN, R. J</creatorcontrib><title>A source of error in phenylketonuria screening</title><title>American journal of clinical pathology</title><addtitle>Am J Clin Pathol</addtitle><description>The escalating number of blood specimens from late-fed premature or very sick newborns greatly increases the risk of missing the diagnosis of phenylketonuria (PKU). Babies receiving antibiotics have uninterpretable "clear-zone" results with the traditional Guthrie bacteriologic inhibition assay (BIA). For the past year the authors have reexamined the blood phenylalanine level on specimens giving the "clear-zone" effect by BIA by use of the McCaman-Robins chemical-fluorescent assay (CFA). Spuriously high blood phenylalanine levels occurred in four babies who were receiving ampicillin and whose specimens were collected on filter paper and autoclaved in preparation for the BIA. None of the babies proved to have PKU. The fluorescent interference caused by ampicillin resulted from the heat of autoclaving the specimen. The authors recommend that the blood specimen should not be autoclaved before analysis by either BIA or CFA.</description><subject>Agar</subject><subject>Aminoacid disorders</subject><subject>Ampicillin - pharmacology</subject><subject>Ampicillin - therapeutic use</subject><subject>Bacillus subtilis - growth & development</subject><subject>Biological and medical sciences</subject><subject>Diffusion</subject><subject>Errors of metabolism</subject><subject>False Positive Reactions</subject><subject>Female</subject><subject>Fluorescence</subject><subject>Hot Temperature</subject><subject>Humans</subject><subject>Infant, Newborn</subject><subject>Infant, Premature</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Metabolic diseases</subject><subject>Phenylalanine - blood</subject><subject>Phenylketonurias - blood</subject><issn>0002-9173</issn><issn>1943-7722</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1988</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kD1PwzAURS0EKqUwMiJlQGxJbT87tseq4kuqxAKz5TgvkJImwW6G_ntSNep0h3t0r3QIuWc0Y9TA0m19vzQ0g4wrc0HmzAhIleL8kswppTw1TME1uYlxSynjmooZmQEDpSnMSbZKYjcEj0lXJRhCF5K6TfofbA_NL-67dgi1S6IPiG3dft-Sq8o1Ee-mXJCvl-fP9Vu6-Xh9X682qQem9mnlmCiVlIppY3KhOdVY5Bolk4oj107wvBDa8wIAyhJN5aQEh8LTwuR5CQvydNrtQ_c3YNzbXR09No1rsRuiVRrM-KRHMD2BPnQxBqxsH-qdCwfLqD36sUc_1lALdvQz8g_T8FDssDzTk5Cxf5x6F71rquBaX8czppjKhQT4B-0mbGs</recordid><startdate>19880901</startdate><enddate>19880901</enddate><creator>MABRY, C. C</creator><creator>REID, M. C</creator><creator>KUHN, R. J</creator><general>American Society of Clinical Pathologists</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19880901</creationdate><title>A source of error in phenylketonuria screening</title><author>MABRY, C. C ; REID, M. C ; KUHN, R. 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C</creatorcontrib><creatorcontrib>REID, M. C</creatorcontrib><creatorcontrib>KUHN, R. J</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>American journal of clinical pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>MABRY, C. C</au><au>REID, M. C</au><au>KUHN, R. 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Spuriously high blood phenylalanine levels occurred in four babies who were receiving ampicillin and whose specimens were collected on filter paper and autoclaved in preparation for the BIA. None of the babies proved to have PKU. The fluorescent interference caused by ampicillin resulted from the heat of autoclaving the specimen. The authors recommend that the blood specimen should not be autoclaved before analysis by either BIA or CFA.</abstract><cop>Chicago, IL</cop><pub>American Society of Clinical Pathologists</pub><pmid>3137803</pmid><doi>10.1093/ajcp/90.3.279</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0002-9173 |
| ispartof | American journal of clinical pathology, 1988-09, Vol.90 (3), p.279-283 |
| issn | 0002-9173 1943-7722 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_78393178 |
| source | Oxford University Press Journals Digital Archive legacy; MEDLINE |
| subjects | Agar Aminoacid disorders Ampicillin - pharmacology Ampicillin - therapeutic use Bacillus subtilis - growth & development Biological and medical sciences Diffusion Errors of metabolism False Positive Reactions Female Fluorescence Hot Temperature Humans Infant, Newborn Infant, Premature Male Medical sciences Metabolic diseases Phenylalanine - blood Phenylketonurias - blood |
| title | A source of error in phenylketonuria screening |
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