Plasmid vectors with multiple cloning sites and cat-reporter gene for promoter cloning and analysis in animal cells
We have constructed two vectors, pGCAT-A and pGCAT-C, designed to facilitate the construction of recombinant plasmids containing the bacterial gene ( cat) coding for chloramphenicol acetyltransferase (CAT) under the control of eukaryotic promoter and/or enhancer elements. The cat gene was inserted d...
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| Veröffentlicht in: | Gene 1988-05, Vol.65 (2), p.315-318 |
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| container_title | Gene |
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| creator | Frebourg, Thierry Brison, Olivier |
| description | We have constructed two vectors, pGCAT-A and pGCAT-C, designed to facilitate the construction of recombinant plasmids containing the bacterial gene (
cat) coding for chloramphenicol acetyltransferase (CAT) under the control of eukaryotic promoter and/or enhancer elements. The
cat gene was inserted downstream from a multiple cloning site (MCS) region with eleven unique restriction sites. The MCS region is in opposite orientation in the two vectors. The CAT activity of control extracts from cells transfected with pGCAT-A or pGCAT-C is very low. Insertion of the viral SV40 early promoter into one of the sites of the MCS region of pGCAT-A or pGCAT-C results in a 30- to 400-fold stimulation of the CAT activity. |
| doi_str_mv | 10.1016/0378-1119(88)90468-4 |
| format | Article |
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cat) coding for chloramphenicol acetyltransferase (CAT) under the control of eukaryotic promoter and/or enhancer elements. The
cat gene was inserted downstream from a multiple cloning site (MCS) region with eleven unique restriction sites. The MCS region is in opposite orientation in the two vectors. The CAT activity of control extracts from cells transfected with pGCAT-A or pGCAT-C is very low. Insertion of the viral SV40 early promoter into one of the sites of the MCS region of pGCAT-A or pGCAT-C results in a 30- to 400-fold stimulation of the CAT activity.</description><identifier>ISSN: 0378-1119</identifier><identifier>EISSN: 1879-0038</identifier><identifier>DOI: 10.1016/0378-1119(88)90468-4</identifier><identifier>PMID: 2842234</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Acetyltransferases - genetics ; Animals ; Base Sequence ; Cells, Cultured ; chloramphenicol acetyl transferase ; Chloramphenicol O-Acetyltransferase ; cloning vectors ; Cloning, Molecular ; DNA transfection ; Genes, Bacterial ; Genetic Vectors ; mammalian cells ; Molecular Sequence Data ; Plasmids ; Promoter Regions, Genetic ; recombinant DNA ; simian virus 40 ; Simian virus 40 - genetics ; Transfection ; transient expression</subject><ispartof>Gene, 1988-05, Vol.65 (2), p.315-318</ispartof><rights>1988</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c388t-765bcc3989c66e6b467dc107204216e5c3c3a76ace48eaff3de7d417a7393d7a3</citedby><cites>FETCH-LOGICAL-c388t-765bcc3989c66e6b467dc107204216e5c3c3a76ace48eaff3de7d417a7393d7a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0378-1119(88)90468-4$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27922,27923,45993</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2842234$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Frebourg, Thierry</creatorcontrib><creatorcontrib>Brison, Olivier</creatorcontrib><title>Plasmid vectors with multiple cloning sites and cat-reporter gene for promoter cloning and analysis in animal cells</title><title>Gene</title><addtitle>Gene</addtitle><description>We have constructed two vectors, pGCAT-A and pGCAT-C, designed to facilitate the construction of recombinant plasmids containing the bacterial gene (
cat) coding for chloramphenicol acetyltransferase (CAT) under the control of eukaryotic promoter and/or enhancer elements. The
cat gene was inserted downstream from a multiple cloning site (MCS) region with eleven unique restriction sites. The MCS region is in opposite orientation in the two vectors. The CAT activity of control extracts from cells transfected with pGCAT-A or pGCAT-C is very low. Insertion of the viral SV40 early promoter into one of the sites of the MCS region of pGCAT-A or pGCAT-C results in a 30- to 400-fold stimulation of the CAT activity.</description><subject>Acetyltransferases - genetics</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Cells, Cultured</subject><subject>chloramphenicol acetyl transferase</subject><subject>Chloramphenicol O-Acetyltransferase</subject><subject>cloning vectors</subject><subject>Cloning, Molecular</subject><subject>DNA transfection</subject><subject>Genes, Bacterial</subject><subject>Genetic Vectors</subject><subject>mammalian cells</subject><subject>Molecular Sequence Data</subject><subject>Plasmids</subject><subject>Promoter Regions, Genetic</subject><subject>recombinant DNA</subject><subject>simian virus 40</subject><subject>Simian virus 40 - genetics</subject><subject>Transfection</subject><subject>transient expression</subject><issn>0378-1119</issn><issn>1879-0038</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1988</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcFPHCEUxkljo6vtf1ATTqYepsLAAHMxaTZVm2yih_ZMWHhjMcyw8mZt_O-d6a4eLRcI7_c9Ht9HyBfOvnHG1QUT2lSc8_arMectk8pU8gNZcKPbijFhDsjiDTkix4gPbFpNUx-Sw9rIuhZyQfAuOexjoE_gx1yQ_o3jH9pv0xg3CahPeYjDPcU4AlI3BOrdWBXY5DJCofcwAO1yoZuS-zzfvApm1A0uPWNEGofpHHuXqIeU8BP52LmE8Hm_n5DfVz9-LW-q1e31z-X3VeWFMWOlVbP2XrSm9UqBWkulg-dM10zWXEHjhRdOK-dBGnBdJwLoILl2WrQiaCdOyNmu7zTd4xZwtH3EeQI3QN6i1Ua03DDxX5A3XCrBZ1DuQF8yYoHObsr0r_JsObNzKHZ23M6OW2Psv1CsnGSn-_7bdQ_hTbRPYapf7uowufEUoVj0EQYPIZYpFhtyfP-BF-W2nTA</recordid><startdate>19880530</startdate><enddate>19880530</enddate><creator>Frebourg, Thierry</creator><creator>Brison, Olivier</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19880530</creationdate><title>Plasmid vectors with multiple cloning sites and cat-reporter gene for promoter cloning and analysis in animal cells</title><author>Frebourg, Thierry ; Brison, Olivier</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c388t-765bcc3989c66e6b467dc107204216e5c3c3a76ace48eaff3de7d417a7393d7a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1988</creationdate><topic>Acetyltransferases - genetics</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Cells, Cultured</topic><topic>chloramphenicol acetyl transferase</topic><topic>Chloramphenicol O-Acetyltransferase</topic><topic>cloning vectors</topic><topic>Cloning, Molecular</topic><topic>DNA transfection</topic><topic>Genes, Bacterial</topic><topic>Genetic Vectors</topic><topic>mammalian cells</topic><topic>Molecular Sequence Data</topic><topic>Plasmids</topic><topic>Promoter Regions, Genetic</topic><topic>recombinant DNA</topic><topic>simian virus 40</topic><topic>Simian virus 40 - genetics</topic><topic>Transfection</topic><topic>transient expression</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Frebourg, Thierry</creatorcontrib><creatorcontrib>Brison, Olivier</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Gene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Frebourg, Thierry</au><au>Brison, Olivier</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Plasmid vectors with multiple cloning sites and cat-reporter gene for promoter cloning and analysis in animal cells</atitle><jtitle>Gene</jtitle><addtitle>Gene</addtitle><date>1988-05-30</date><risdate>1988</risdate><volume>65</volume><issue>2</issue><spage>315</spage><epage>318</epage><pages>315-318</pages><issn>0378-1119</issn><eissn>1879-0038</eissn><abstract>We have constructed two vectors, pGCAT-A and pGCAT-C, designed to facilitate the construction of recombinant plasmids containing the bacterial gene (
cat) coding for chloramphenicol acetyltransferase (CAT) under the control of eukaryotic promoter and/or enhancer elements. The
cat gene was inserted downstream from a multiple cloning site (MCS) region with eleven unique restriction sites. The MCS region is in opposite orientation in the two vectors. The CAT activity of control extracts from cells transfected with pGCAT-A or pGCAT-C is very low. Insertion of the viral SV40 early promoter into one of the sites of the MCS region of pGCAT-A or pGCAT-C results in a 30- to 400-fold stimulation of the CAT activity.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>2842234</pmid><doi>10.1016/0378-1119(88)90468-4</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0378-1119 |
| ispartof | Gene, 1988-05, Vol.65 (2), p.315-318 |
| issn | 0378-1119 1879-0038 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_78391803 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Acetyltransferases - genetics Animals Base Sequence Cells, Cultured chloramphenicol acetyl transferase Chloramphenicol O-Acetyltransferase cloning vectors Cloning, Molecular DNA transfection Genes, Bacterial Genetic Vectors mammalian cells Molecular Sequence Data Plasmids Promoter Regions, Genetic recombinant DNA simian virus 40 Simian virus 40 - genetics Transfection transient expression |
| title | Plasmid vectors with multiple cloning sites and cat-reporter gene for promoter cloning and analysis in animal cells |
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