The amplified detection of free and bound methoxatin (PQQ) with nitroblue tetrazolium redox reactions: insights into the PQQ-locus

Porcine kidney diamine oxidase, a PQQ-enzyme, can be directly measured by formazan production with putrescine and nitroblue tetrazolium. This cyclic reaction in air is unaffected by superoxide dimutase, suggesting a two electron transfer between substrate-reduced PQQ-locus and nitroblue tetrazolium,...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Biochemical and biophysical research communications 1988-08, Vol.154 (3), p.1330-1337
Hauptverfasser:	PAZ, M. A, GALLOP, P. M, TORRELIO, B. M, FLÜCKIGER, R
Format:	Artikel
Sprache:	eng
Schlagworte:	Amine Oxidase (Copper-Containing) - metabolism Analytical, structural and metabolic biochemistry Animals Biological and medical sciences enzymes Enzymes and enzyme inhibitors Fundamental and applied biological sciences. Psychology Indicators and Reagents Kidney - enzymology Kinetics methoxatin Microchemistry Nitroblue Tetrazolium Oxidation-Reduction Oxidoreductases PQQ Cofactor Protein Binding Putrescine - metabolism pyrroloquinoline quinone Quinolines - analysis Quinolines - metabolism redox potential Spectrophotometry - methods Swine
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!