Expression of tumor necrosis factor alpha after focal cerebral ischaemia in the rat

Induction of tumor necrosis factor α was studied in the brain of rats after focal cerebral ischaemia by occlusion of the left middle cerebral artery. Using a specific antisense riboprobe for situ hybridization histochemistry, cells positive for tumor necrosis factor α messenger RNA were detected wit...

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Veröffentlicht in:	Neuroscience 1996-03, Vol.71 (1), p.1-16
Hauptverfasser:	Buttini, M., Appel, K., Sauter, A., Gebicke-Haerter, P.-J., Boddeke, H.W.G.M.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Base Sequence Blotting, Western Brain Chemistry - physiology Cerebral Arteries - physiology Cloning, Molecular cytokines focal cerebral ischaemia Immunohistochemistry In Situ Hybridization inflammatory reactions Ischemic Attack, Transient - metabolism macrophages Male microglia Microglia - metabolism Molecular Sequence Data Oligonucleotides, Antisense - chemical synthesis Polymerase Chain Reaction Rats Rats, Inbred SHR RNA Probes RNA, Messenger - biosynthesis TNFα Tumor Necrosis Factor-alpha - biosynthesis
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