4-(N-Hydrazinoformylmethyl-N-Methyl)-Amino-7-N,N-Dimethylaminosulphonyl-2,1,3-Benzoxadiazole (DBD-CO-Hz) as a Precolumn Fluorescence Derivatization Reagent for Carboxylic Acids in High-Performance Liquid Chromatography
A new fluorescent reagent for carboxylic acids, 4‐(N‐hydrazinoformylmethyl‐N‐methyl)amino‐7‐N,N‐dimethylaminosulphonyl‐2,1,3‐benzoxadiazole (DBD‐CO‐Hz) was synthesized and its applicability as a precolumn derivatization reagent in high‐performance liquid chromatography (HPLC) was examined. DBD‐CO‐Hz...
Gespeichert in:
| Veröffentlicht in: | Biomedical chromatography 1996-07, Vol.10 (4), p.183-185 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 185 |
|---|---|
| container_issue | 4 |
| container_start_page | 183 |
| container_title | Biomedical chromatography |
| container_volume | 10 |
| creator | Santa, Tomofumi Kimoto, Kousuke Fukushima, Takeshi Homma, Hiroshi Imai, Kazuhiro |
| description | A new fluorescent reagent for carboxylic acids, 4‐(N‐hydrazinoformylmethyl‐N‐methyl)amino‐7‐N,N‐dimethylaminosulphonyl‐2,1,3‐benzoxadiazole (DBD‐CO‐Hz) was synthesized and its applicability as a precolumn derivatization reagent in high‐performance liquid chromatography (HPLC) was examined.
DBD‐CO‐Hz reacted with saturated fatty acids in the presence of a condensing agent, 1‐ethyl‐3‐(3‐dimethylaminopropyl)carbodiimide (EDC) and pyridine at room temperature for 30 min to give fluorescent products. The reaction solution was subjected to a reversed‐phase HPLC and detected fluorometrically at a wavelength of 550 nm with an excitation of 440 nm. The detection limits of the derivatives were at low fmol range on column. |
| doi_str_mv | 10.1002/(SICI)1099-0801(199607)10:4<183::AID-BMC581>3.0.CO;2-I |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_78380452</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>78380452</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3171-506da0fae62055e723a181182b93030819e4f0a60f87d879aef3e1da2865e8d23</originalsourceid><addsrcrecordid>eNqFkd2O0zAQhSMEWsrCIyD5CrVSXey4SZwFrdSm7CZSN612-buz3MRpDUnctRNo-qg8DQmpegMSF5atOTNnZvxZ1jVGE4yQ_Xb4EAXRCCPfh4giPMS-7yKvDVxN32NKrq5m0QLO7wKH4msyQZNg9c6G0RNrcC55ag2Q7fqQUM9_br0w5htCyHdt78K6oJRg3yUD69cUDmMYNqnmR1mqTOmiyQtR7ZocxvDuz2MEZ0WrQQ_G4xguZC_zLmbqfL9TZZtsj_GYwLkoj-rAU8mPKhdguJgvYLCC4XEEuAEcrLVIVF4XJbjJa6WFSUSZCLAQWv7glTy2R5XgXvCtKCvQTgMCrjfq0OQyAbNEpgbIEoRyu4NrobtpeVe_lI-1TEGw06rgldpqvt81L61nGc-NeHW6L61PNx8-BiFcrm6jYLaECcEehg5yU44yLlwbOY7wbMIxxZjaG58ggij2xTRD3EUZ9dL2K7nIiMApt6nrCJra5NJ60_vutXqshalYIdu98pyXQtWGeZRQNHW6xM99YqKVMVpkbK9lwXXDMGIddMY66KwjyDqCrIfeyVPWQmeshc566IwwxIIVs1nUGr8-TVBvCpGebU-UW_1rr_-UuWj-6vqfpv_seYq01rC3lqYSh7M119-Z6xHPYV_iW4bDeL28fwgZIb8BBwnY-w</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>78380452</pqid></control><display><type>article</type><title>4-(N-Hydrazinoformylmethyl-N-Methyl)-Amino-7-N,N-Dimethylaminosulphonyl-2,1,3-Benzoxadiazole (DBD-CO-Hz) as a Precolumn Fluorescence Derivatization Reagent for Carboxylic Acids in High-Performance Liquid Chromatography</title><source>MEDLINE</source><source>Wiley Online Library All Journals</source><creator>Santa, Tomofumi ; Kimoto, Kousuke ; Fukushima, Takeshi ; Homma, Hiroshi ; Imai, Kazuhiro</creator><creatorcontrib>Santa, Tomofumi ; Kimoto, Kousuke ; Fukushima, Takeshi ; Homma, Hiroshi ; Imai, Kazuhiro</creatorcontrib><description>A new fluorescent reagent for carboxylic acids, 4‐(N‐hydrazinoformylmethyl‐N‐methyl)amino‐7‐N,N‐dimethylaminosulphonyl‐2,1,3‐benzoxadiazole (DBD‐CO‐Hz) was synthesized and its applicability as a precolumn derivatization reagent in high‐performance liquid chromatography (HPLC) was examined.
DBD‐CO‐Hz reacted with saturated fatty acids in the presence of a condensing agent, 1‐ethyl‐3‐(3‐dimethylaminopropyl)carbodiimide (EDC) and pyridine at room temperature for 30 min to give fluorescent products. The reaction solution was subjected to a reversed‐phase HPLC and detected fluorometrically at a wavelength of 550 nm with an excitation of 440 nm. The detection limits of the derivatives were at low fmol range on column.</description><identifier>ISSN: 0269-3879</identifier><identifier>EISSN: 1099-0801</identifier><identifier>DOI: 10.1002/(SICI)1099-0801(199607)10:4<183::AID-BMC581>3.0.CO;2-I</identifier><identifier>PMID: 8831963</identifier><language>eng</language><publisher>Chichester, UK: John Wiley & Sons, Ltd</publisher><subject>Carboxylic Acids - chemistry ; Chromatography, High Pressure Liquid ; Fatty Acids - analysis ; Fatty Acids - chemistry ; Fluorescent Dyes - chemistry ; Oxadiazoles - chemistry ; Spectrometry, Fluorescence</subject><ispartof>Biomedical chromatography, 1996-07, Vol.10 (4), p.183-185</ispartof><rights>Copyright © 1996 John Wiley & Sons, Ltd.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c3171-506da0fae62055e723a181182b93030819e4f0a60f87d879aef3e1da2865e8d23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2F%28SICI%291099-0801%28199607%2910%3A4%3C183%3A%3AAID-BMC581%3E3.0.CO%3B2-I$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2F%28SICI%291099-0801%28199607%2910%3A4%3C183%3A%3AAID-BMC581%3E3.0.CO%3B2-I$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1416,27922,27923,45572,45573</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8831963$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Santa, Tomofumi</creatorcontrib><creatorcontrib>Kimoto, Kousuke</creatorcontrib><creatorcontrib>Fukushima, Takeshi</creatorcontrib><creatorcontrib>Homma, Hiroshi</creatorcontrib><creatorcontrib>Imai, Kazuhiro</creatorcontrib><title>4-(N-Hydrazinoformylmethyl-N-Methyl)-Amino-7-N,N-Dimethylaminosulphonyl-2,1,3-Benzoxadiazole (DBD-CO-Hz) as a Precolumn Fluorescence Derivatization Reagent for Carboxylic Acids in High-Performance Liquid Chromatography</title><title>Biomedical chromatography</title><addtitle>Biomed. Chromatogr</addtitle><description>A new fluorescent reagent for carboxylic acids, 4‐(N‐hydrazinoformylmethyl‐N‐methyl)amino‐7‐N,N‐dimethylaminosulphonyl‐2,1,3‐benzoxadiazole (DBD‐CO‐Hz) was synthesized and its applicability as a precolumn derivatization reagent in high‐performance liquid chromatography (HPLC) was examined.
DBD‐CO‐Hz reacted with saturated fatty acids in the presence of a condensing agent, 1‐ethyl‐3‐(3‐dimethylaminopropyl)carbodiimide (EDC) and pyridine at room temperature for 30 min to give fluorescent products. The reaction solution was subjected to a reversed‐phase HPLC and detected fluorometrically at a wavelength of 550 nm with an excitation of 440 nm. The detection limits of the derivatives were at low fmol range on column.</description><subject>Carboxylic Acids - chemistry</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Fatty Acids - analysis</subject><subject>Fatty Acids - chemistry</subject><subject>Fluorescent Dyes - chemistry</subject><subject>Oxadiazoles - chemistry</subject><subject>Spectrometry, Fluorescence</subject><issn>0269-3879</issn><issn>1099-0801</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkd2O0zAQhSMEWsrCIyD5CrVSXey4SZwFrdSm7CZSN612-buz3MRpDUnctRNo-qg8DQmpegMSF5atOTNnZvxZ1jVGE4yQ_Xb4EAXRCCPfh4giPMS-7yKvDVxN32NKrq5m0QLO7wKH4msyQZNg9c6G0RNrcC55ag2Q7fqQUM9_br0w5htCyHdt78K6oJRg3yUD69cUDmMYNqnmR1mqTOmiyQtR7ZocxvDuz2MEZ0WrQQ_G4xguZC_zLmbqfL9TZZtsj_GYwLkoj-rAU8mPKhdguJgvYLCC4XEEuAEcrLVIVF4XJbjJa6WFSUSZCLAQWv7glTy2R5XgXvCtKCvQTgMCrjfq0OQyAbNEpgbIEoRyu4NrobtpeVe_lI-1TEGw06rgldpqvt81L61nGc-NeHW6L61PNx8-BiFcrm6jYLaECcEehg5yU44yLlwbOY7wbMIxxZjaG58ggij2xTRD3EUZ9dL2K7nIiMApt6nrCJra5NJ60_vutXqshalYIdu98pyXQtWGeZRQNHW6xM99YqKVMVpkbK9lwXXDMGIddMY66KwjyDqCrIfeyVPWQmeshc566IwwxIIVs1nUGr8-TVBvCpGebU-UW_1rr_-UuWj-6vqfpv_seYq01rC3lqYSh7M119-Z6xHPYV_iW4bDeL28fwgZIb8BBwnY-w</recordid><startdate>199607</startdate><enddate>199607</enddate><creator>Santa, Tomofumi</creator><creator>Kimoto, Kousuke</creator><creator>Fukushima, Takeshi</creator><creator>Homma, Hiroshi</creator><creator>Imai, Kazuhiro</creator><general>John Wiley & Sons, Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199607</creationdate><title>4-(N-Hydrazinoformylmethyl-N-Methyl)-Amino-7-N,N-Dimethylaminosulphonyl-2,1,3-Benzoxadiazole (DBD-CO-Hz) as a Precolumn Fluorescence Derivatization Reagent for Carboxylic Acids in High-Performance Liquid Chromatography</title><author>Santa, Tomofumi ; Kimoto, Kousuke ; Fukushima, Takeshi ; Homma, Hiroshi ; Imai, Kazuhiro</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3171-506da0fae62055e723a181182b93030819e4f0a60f87d879aef3e1da2865e8d23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Carboxylic Acids - chemistry</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Fatty Acids - analysis</topic><topic>Fatty Acids - chemistry</topic><topic>Fluorescent Dyes - chemistry</topic><topic>Oxadiazoles - chemistry</topic><topic>Spectrometry, Fluorescence</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Santa, Tomofumi</creatorcontrib><creatorcontrib>Kimoto, Kousuke</creatorcontrib><creatorcontrib>Fukushima, Takeshi</creatorcontrib><creatorcontrib>Homma, Hiroshi</creatorcontrib><creatorcontrib>Imai, Kazuhiro</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biomedical chromatography</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Santa, Tomofumi</au><au>Kimoto, Kousuke</au><au>Fukushima, Takeshi</au><au>Homma, Hiroshi</au><au>Imai, Kazuhiro</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>4-(N-Hydrazinoformylmethyl-N-Methyl)-Amino-7-N,N-Dimethylaminosulphonyl-2,1,3-Benzoxadiazole (DBD-CO-Hz) as a Precolumn Fluorescence Derivatization Reagent for Carboxylic Acids in High-Performance Liquid Chromatography</atitle><jtitle>Biomedical chromatography</jtitle><addtitle>Biomed. Chromatogr</addtitle><date>1996-07</date><risdate>1996</risdate><volume>10</volume><issue>4</issue><spage>183</spage><epage>185</epage><pages>183-185</pages><issn>0269-3879</issn><eissn>1099-0801</eissn><abstract>A new fluorescent reagent for carboxylic acids, 4‐(N‐hydrazinoformylmethyl‐N‐methyl)amino‐7‐N,N‐dimethylaminosulphonyl‐2,1,3‐benzoxadiazole (DBD‐CO‐Hz) was synthesized and its applicability as a precolumn derivatization reagent in high‐performance liquid chromatography (HPLC) was examined.
DBD‐CO‐Hz reacted with saturated fatty acids in the presence of a condensing agent, 1‐ethyl‐3‐(3‐dimethylaminopropyl)carbodiimide (EDC) and pyridine at room temperature for 30 min to give fluorescent products. The reaction solution was subjected to a reversed‐phase HPLC and detected fluorometrically at a wavelength of 550 nm with an excitation of 440 nm. The detection limits of the derivatives were at low fmol range on column.</abstract><cop>Chichester, UK</cop><pub>John Wiley & Sons, Ltd</pub><pmid>8831963</pmid><doi>10.1002/(SICI)1099-0801(199607)10:4<183::AID-BMC581>3.0.CO;2-I</doi><tpages>3</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0269-3879 |
| ispartof | Biomedical chromatography, 1996-07, Vol.10 (4), p.183-185 |
| issn | 0269-3879 1099-0801 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_78380452 |
| source | MEDLINE; Wiley Online Library All Journals |
| subjects | Carboxylic Acids - chemistry Chromatography, High Pressure Liquid Fatty Acids - analysis Fatty Acids - chemistry Fluorescent Dyes - chemistry Oxadiazoles - chemistry Spectrometry, Fluorescence |
| title | 4-(N-Hydrazinoformylmethyl-N-Methyl)-Amino-7-N,N-Dimethylaminosulphonyl-2,1,3-Benzoxadiazole (DBD-CO-Hz) as a Precolumn Fluorescence Derivatization Reagent for Carboxylic Acids in High-Performance Liquid Chromatography |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-14T00%3A18%3A00IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=4-(N-Hydrazinoformylmethyl-N-Methyl)-Amino-7-N,N-Dimethylaminosulphonyl-2,1,3-Benzoxadiazole%20(DBD-CO-Hz)%20as%20a%20Precolumn%20Fluorescence%20Derivatization%20Reagent%20for%20Carboxylic%20Acids%20in%20High-Performance%20Liquid%20Chromatography&rft.jtitle=Biomedical%20chromatography&rft.au=Santa,%20Tomofumi&rft.date=1996-07&rft.volume=10&rft.issue=4&rft.spage=183&rft.epage=185&rft.pages=183-185&rft.issn=0269-3879&rft.eissn=1099-0801&rft_id=info:doi/10.1002/(SICI)1099-0801(199607)10:4%3C183::AID-BMC581%3E3.0.CO;2-I&rft_dat=%3Cproquest_cross%3E78380452%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=78380452&rft_id=info:pmid/8831963&rfr_iscdi=true |