Further evidence for the presence of cannabinoid CB1 receptors in mouse vas deferens
Our results provide further evidence for the hypothesis that the mouse vas deferens contains cannabinoid CB1 receptors. Thus we found that in the presence of forskolin, the cannabinoid receptor agonist, CP 55,940 ((-)-3-[2-hydroxy-4-(1,1-dimethyl-heptyl)phenyl]-4-(3- hydroxypropyl)cyclohexan-1-ol) p...
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| Veröffentlicht in: | European journal of pharmacology 1996-01, Vol.296 (2), p.169-172 |
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| container_title | European journal of pharmacology |
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| creator | PERTWEE, R. G JOE-ADIGWE, G HAWKSWORTH, G. M |
| description | Our results provide further evidence for the hypothesis that the mouse vas deferens contains cannabinoid CB1 receptors. Thus we found that in the presence of forskolin, the cannabinoid receptor agonist, CP 55,940 ((-)-3-[2-hydroxy-4-(1,1-dimethyl-heptyl)phenyl]-4-(3- hydroxypropyl)cyclohexan-1-ol) produced a concentration related inhibition of cyclic AMP production by the vas deferens (EC50 = 6.0 nM). At 100 nM, SR141716A (N-(piperidin-1-yl)-5-(4-chlorophenyl)-1-(2,4-dichlorophenyl)-4-me thyl-1H- pyrazole-3-carboxamide hydrochloride) attenuated this effect of CP 55,940, producing a parallel rightward shift in its log concentration-response curve (Kd = 4.3 nM). We also found that cyclic AMP production was inhibited by (-)-11-hydroxy-1',1'-dimethylheptyl-delta 8- tetrahydrocannabinol but not by the (+) enantiomer. |
| doi_str_mv | 10.1016/0014-2999(95)00790-3 |
| format | Article |
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G ; JOE-ADIGWE, G ; HAWKSWORTH, G. M</creator><creatorcontrib>PERTWEE, R. G ; JOE-ADIGWE, G ; HAWKSWORTH, G. M</creatorcontrib><description>Our results provide further evidence for the hypothesis that the mouse vas deferens contains cannabinoid CB1 receptors. Thus we found that in the presence of forskolin, the cannabinoid receptor agonist, CP 55,940 ((-)-3-[2-hydroxy-4-(1,1-dimethyl-heptyl)phenyl]-4-(3- hydroxypropyl)cyclohexan-1-ol) produced a concentration related inhibition of cyclic AMP production by the vas deferens (EC50 = 6.0 nM). At 100 nM, SR141716A (N-(piperidin-1-yl)-5-(4-chlorophenyl)-1-(2,4-dichlorophenyl)-4-me thyl-1H- pyrazole-3-carboxamide hydrochloride) attenuated this effect of CP 55,940, producing a parallel rightward shift in its log concentration-response curve (Kd = 4.3 nM). We also found that cyclic AMP production was inhibited by (-)-11-hydroxy-1',1'-dimethylheptyl-delta 8- tetrahydrocannabinol but not by the (+) enantiomer.</description><identifier>ISSN: 0014-2999</identifier><identifier>EISSN: 1879-0712</identifier><identifier>DOI: 10.1016/0014-2999(95)00790-3</identifier><identifier>PMID: 8838453</identifier><identifier>CODEN: EJPHAZ</identifier><language>eng</language><publisher>Amsterdam: Elsevier</publisher><subject>Animals ; Biological and medical sciences ; Cannabinoids - metabolism ; Colforsin - pharmacology ; Cyclic AMP - biosynthesis ; Cyclohexanols - pharmacology ; Fundamental and applied biological sciences. 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G</creatorcontrib><creatorcontrib>JOE-ADIGWE, G</creatorcontrib><creatorcontrib>HAWKSWORTH, G. M</creatorcontrib><title>Further evidence for the presence of cannabinoid CB1 receptors in mouse vas deferens</title><title>European journal of pharmacology</title><addtitle>Eur J Pharmacol</addtitle><description>Our results provide further evidence for the hypothesis that the mouse vas deferens contains cannabinoid CB1 receptors. Thus we found that in the presence of forskolin, the cannabinoid receptor agonist, CP 55,940 ((-)-3-[2-hydroxy-4-(1,1-dimethyl-heptyl)phenyl]-4-(3- hydroxypropyl)cyclohexan-1-ol) produced a concentration related inhibition of cyclic AMP production by the vas deferens (EC50 = 6.0 nM). At 100 nM, SR141716A (N-(piperidin-1-yl)-5-(4-chlorophenyl)-1-(2,4-dichlorophenyl)-4-me thyl-1H- pyrazole-3-carboxamide hydrochloride) attenuated this effect of CP 55,940, producing a parallel rightward shift in its log concentration-response curve (Kd = 4.3 nM). We also found that cyclic AMP production was inhibited by (-)-11-hydroxy-1',1'-dimethylheptyl-delta 8- tetrahydrocannabinol but not by the (+) enantiomer.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cannabinoids - metabolism</subject><subject>Colforsin - pharmacology</subject><subject>Cyclic AMP - biosynthesis</subject><subject>Cyclohexanols - pharmacology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hormone metabolism and regulation</subject><subject>In Vitro Techniques</subject><subject>Male</subject><subject>Mammalian male genital system</subject><subject>Mice</subject><subject>Piperidines - pharmacology</subject><subject>Pyrazoles - pharmacology</subject><subject>Receptors, Cannabinoid</subject><subject>Receptors, Drug - agonists</subject><subject>Receptors, Drug - antagonists & inhibitors</subject><subject>Receptors, Drug - metabolism</subject><subject>Stereoisomerism</subject><subject>Vas Deferens - drug effects</subject><subject>Vas Deferens - metabolism</subject><subject>Vertebrates: reproduction</subject><issn>0014-2999</issn><issn>1879-0712</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kEtPwzAQhC0EKqXwD0DyASE4BHbjOLaPUPGSKnHp3XKcjQhqHthNJf496UM9rTQzO7v6GLtGeETA_AkAsyQ1xtwb-QCgDCTihE1RK5OAwvSUTY-Rc3YR4w8ASJPKCZtoLXQmxZQt34aw_qbAaVOX1HriVRf4qPA-UNwJXcW9a1tX1G1Xl3z-gjyQp37dhcjrljfdEIlvXOQlVRSojZfsrHKrSFeHORvPvC7nH8ni6_1z_rxIvBC4TkRRCim1r3SuEEk556RGDRqLTBaY59I4SaXG3BmlM5NqDzkigK4oy0DM2N2-tg_d70BxbZs6elqtXEvjT1ZpoYwCPQazfdCHLsZAle1D3bjwZxHslqXdgrJbUNZIu2Npxbh2c-gfiobK49IB3ujfHnwXvVtVwbW-jseYGDvV-OY_nVZ6ZQ</recordid><startdate>19960125</startdate><enddate>19960125</enddate><creator>PERTWEE, R. G</creator><creator>JOE-ADIGWE, G</creator><creator>HAWKSWORTH, G. M</creator><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19960125</creationdate><title>Further evidence for the presence of cannabinoid CB1 receptors in mouse vas deferens</title><author>PERTWEE, R. G ; JOE-ADIGWE, G ; HAWKSWORTH, G. M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c331t-3bd3558cf86711e7aaa5818081b45b16659a5ed816a9784928c0611008fe4403</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cannabinoids - metabolism</topic><topic>Colforsin - pharmacology</topic><topic>Cyclic AMP - biosynthesis</topic><topic>Cyclohexanols - pharmacology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hormone metabolism and regulation</topic><topic>In Vitro Techniques</topic><topic>Male</topic><topic>Mammalian male genital system</topic><topic>Mice</topic><topic>Piperidines - pharmacology</topic><topic>Pyrazoles - pharmacology</topic><topic>Receptors, Cannabinoid</topic><topic>Receptors, Drug - agonists</topic><topic>Receptors, Drug - antagonists & inhibitors</topic><topic>Receptors, Drug - metabolism</topic><topic>Stereoisomerism</topic><topic>Vas Deferens - drug effects</topic><topic>Vas Deferens - metabolism</topic><topic>Vertebrates: reproduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>PERTWEE, R. G</creatorcontrib><creatorcontrib>JOE-ADIGWE, G</creatorcontrib><creatorcontrib>HAWKSWORTH, G. M</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>European journal of pharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>PERTWEE, R. G</au><au>JOE-ADIGWE, G</au><au>HAWKSWORTH, G. M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Further evidence for the presence of cannabinoid CB1 receptors in mouse vas deferens</atitle><jtitle>European journal of pharmacology</jtitle><addtitle>Eur J Pharmacol</addtitle><date>1996-01-25</date><risdate>1996</risdate><volume>296</volume><issue>2</issue><spage>169</spage><epage>172</epage><pages>169-172</pages><issn>0014-2999</issn><eissn>1879-0712</eissn><coden>EJPHAZ</coden><abstract>Our results provide further evidence for the hypothesis that the mouse vas deferens contains cannabinoid CB1 receptors. Thus we found that in the presence of forskolin, the cannabinoid receptor agonist, CP 55,940 ((-)-3-[2-hydroxy-4-(1,1-dimethyl-heptyl)phenyl]-4-(3- hydroxypropyl)cyclohexan-1-ol) produced a concentration related inhibition of cyclic AMP production by the vas deferens (EC50 = 6.0 nM). At 100 nM, SR141716A (N-(piperidin-1-yl)-5-(4-chlorophenyl)-1-(2,4-dichlorophenyl)-4-me thyl-1H- pyrazole-3-carboxamide hydrochloride) attenuated this effect of CP 55,940, producing a parallel rightward shift in its log concentration-response curve (Kd = 4.3 nM). We also found that cyclic AMP production was inhibited by (-)-11-hydroxy-1',1'-dimethylheptyl-delta 8- tetrahydrocannabinol but not by the (+) enantiomer.</abstract><cop>Amsterdam</cop><pub>Elsevier</pub><pmid>8838453</pmid><doi>10.1016/0014-2999(95)00790-3</doi><tpages>4</tpages></addata></record> |
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| ispartof | European journal of pharmacology, 1996-01, Vol.296 (2), p.169-172 |
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| language | eng |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Animals Biological and medical sciences Cannabinoids - metabolism Colforsin - pharmacology Cyclic AMP - biosynthesis Cyclohexanols - pharmacology Fundamental and applied biological sciences. Psychology Hormone metabolism and regulation In Vitro Techniques Male Mammalian male genital system Mice Piperidines - pharmacology Pyrazoles - pharmacology Receptors, Cannabinoid Receptors, Drug - agonists Receptors, Drug - antagonists & inhibitors Receptors, Drug - metabolism Stereoisomerism Vas Deferens - drug effects Vas Deferens - metabolism Vertebrates: reproduction |
| title | Further evidence for the presence of cannabinoid CB1 receptors in mouse vas deferens |
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