Enhanced lipid peroxidation by extracellular ATP in PC12 cells
Recently we have demonstrated that extracellular ATP acts as an excitatory neurotransmitter and enhances cell death in the presence of ferrous ions. By using a newly developed cis-parinaric acid fluorescence technique, we demonstrated that ATP, in a dose dependent manner, enhanced the increased memb...
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| Veröffentlicht in: | Neurochemical research 1996, Vol.21 (1), p.27-33 |
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| container_title | Neurochemical research |
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| creator | CHENG, Y CHEN, M JAMES-KRACKE, M WIXOM, P SUN, A. Y |
| description | Recently we have demonstrated that extracellular ATP acts as an excitatory neurotransmitter and enhances cell death in the presence of ferrous ions. By using a newly developed cis-parinaric acid fluorescence technique, we demonstrated that ATP, in a dose dependent manner, enhanced the increased membrane lipid peroxidation in PC12 cells when cells were incubated with micromolar FeCl2/DTP. P2 purinoceptor agonists, alpha,beta-methylene ATP and 2-methylthio-ATP, induced PC12 cell lipid peroxidation, but to a lesser extent than ATP. ATP-induced Ca(2+) influx via P2 purinoceptor activation significantly increased the intracellular Ca(2+)concentration, which may have triggered a free radical generating cascade(s), and led to membrane lipid peroxidation and cell death. Since oxidative stress has been implicated in certain neurodegenerative diseases such as aging, extracellular ATP may contribute to neuronal cell death by an oxidative mechanism involving lipid peroxidation. |
| doi_str_mv | 10.1007/BF02527668 |
| format | Article |
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Since oxidative stress has been implicated in certain neurodegenerative diseases such as aging, extracellular ATP may contribute to neuronal cell death by an oxidative mechanism involving lipid peroxidation.</description><identifier>ISSN: 0364-3190</identifier><identifier>EISSN: 1573-6903</identifier><identifier>DOI: 10.1007/BF02527668</identifier><identifier>PMID: 8833220</identifier><identifier>CODEN: NEREDZ</identifier><language>eng</language><publisher>New York, NY: Springer</publisher><subject>Adenosine Triphosphate - physiology ; Ageing, cell death ; Animals ; Biological and medical sciences ; Cell Death - physiology ; Cell physiology ; Fatty Acids, Unsaturated ; Fluorescent Dyes ; Free Radicals ; Fundamental and applied biological sciences. 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Y</creatorcontrib><title>Enhanced lipid peroxidation by extracellular ATP in PC12 cells</title><title>Neurochemical research</title><addtitle>Neurochem Res</addtitle><description>Recently we have demonstrated that extracellular ATP acts as an excitatory neurotransmitter and enhances cell death in the presence of ferrous ions. By using a newly developed cis-parinaric acid fluorescence technique, we demonstrated that ATP, in a dose dependent manner, enhanced the increased membrane lipid peroxidation in PC12 cells when cells were incubated with micromolar FeCl2/DTP. P2 purinoceptor agonists, alpha,beta-methylene ATP and 2-methylthio-ATP, induced PC12 cell lipid peroxidation, but to a lesser extent than ATP. ATP-induced Ca(2+) influx via P2 purinoceptor activation significantly increased the intracellular Ca(2+)concentration, which may have triggered a free radical generating cascade(s), and led to membrane lipid peroxidation and cell death. Since oxidative stress has been implicated in certain neurodegenerative diseases such as aging, extracellular ATP may contribute to neuronal cell death by an oxidative mechanism involving lipid peroxidation.</description><subject>Adenosine Triphosphate - physiology</subject><subject>Ageing, cell death</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cell Death - physiology</subject><subject>Cell physiology</subject><subject>Fatty Acids, Unsaturated</subject><subject>Fluorescent Dyes</subject><subject>Free Radicals</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Lipid Peroxidation - physiology</subject><subject>Molecular and cellular biology</subject><subject>PC12 Cells</subject><subject>Rats</subject><subject>Receptors, Purinergic P2 - physiology</subject><subject>Stereoisomerism</subject><issn>0364-3190</issn><issn>1573-6903</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1Lw0AQhhdRaq1evAs5iAchOrub7G4uQi2tCgV7qOewX8GVNIm7CbT_3oQGPXoamPfhneFB6BrDAwbgj88rICnhjIkTNMUppzHLgJ6iKVCWxBRncI4uQvgC6HGCJ2giBKWEwBQ9LatPWWlrotI1zkSN9fXeGdm6uorUIbL71ktty7IrpY_m203kqmizwCQaluESnRWyDPZqnDP0sVpuF6_x-v3lbTFfx5ompI1TsEIVSmYFZ0YIowpONFZKK8KENtYmYDKSCU4gZYolhbXKcJKoQlqbUUJn6O7Y2_j6u7OhzXcuDB_IytZdyLmgHEDwf0HM-wucDuD9EdS-DsHbIm-820l_yDHkg9X8z2oP34ytndpZ84uOGvv8dsxl0LIsfK_UhV-MAmEpUPoDY919SA</recordid><startdate>1996</startdate><enddate>1996</enddate><creator>CHENG, Y</creator><creator>CHEN, M</creator><creator>JAMES-KRACKE, M</creator><creator>WIXOM, P</creator><creator>SUN, A. 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Psychology</topic><topic>Lipid Peroxidation - physiology</topic><topic>Molecular and cellular biology</topic><topic>PC12 Cells</topic><topic>Rats</topic><topic>Receptors, Purinergic P2 - physiology</topic><topic>Stereoisomerism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>CHENG, Y</creatorcontrib><creatorcontrib>CHEN, M</creatorcontrib><creatorcontrib>JAMES-KRACKE, M</creatorcontrib><creatorcontrib>WIXOM, P</creatorcontrib><creatorcontrib>SUN, A. 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Y</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Enhanced lipid peroxidation by extracellular ATP in PC12 cells</atitle><jtitle>Neurochemical research</jtitle><addtitle>Neurochem Res</addtitle><date>1996</date><risdate>1996</risdate><volume>21</volume><issue>1</issue><spage>27</spage><epage>33</epage><pages>27-33</pages><issn>0364-3190</issn><eissn>1573-6903</eissn><coden>NEREDZ</coden><abstract>Recently we have demonstrated that extracellular ATP acts as an excitatory neurotransmitter and enhances cell death in the presence of ferrous ions. By using a newly developed cis-parinaric acid fluorescence technique, we demonstrated that ATP, in a dose dependent manner, enhanced the increased membrane lipid peroxidation in PC12 cells when cells were incubated with micromolar FeCl2/DTP. P2 purinoceptor agonists, alpha,beta-methylene ATP and 2-methylthio-ATP, induced PC12 cell lipid peroxidation, but to a lesser extent than ATP. ATP-induced Ca(2+) influx via P2 purinoceptor activation significantly increased the intracellular Ca(2+)concentration, which may have triggered a free radical generating cascade(s), and led to membrane lipid peroxidation and cell death. Since oxidative stress has been implicated in certain neurodegenerative diseases such as aging, extracellular ATP may contribute to neuronal cell death by an oxidative mechanism involving lipid peroxidation.</abstract><cop>New York, NY</cop><pub>Springer</pub><pmid>8833220</pmid><doi>10.1007/BF02527668</doi><tpages>7</tpages></addata></record> |
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| ispartof | Neurochemical research, 1996, Vol.21 (1), p.27-33 |
| issn | 0364-3190 1573-6903 |
| language | eng |
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| source | MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | Adenosine Triphosphate - physiology Ageing, cell death Animals Biological and medical sciences Cell Death - physiology Cell physiology Fatty Acids, Unsaturated Fluorescent Dyes Free Radicals Fundamental and applied biological sciences. Psychology Lipid Peroxidation - physiology Molecular and cellular biology PC12 Cells Rats Receptors, Purinergic P2 - physiology Stereoisomerism |
| title | Enhanced lipid peroxidation by extracellular ATP in PC12 cells |
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