Synchronous Oscillations in Cerebrocortical Capillary Red Blood Cell Velocity after Nitric Oxide Synthase Inhibition
Low-frequency (4–12 cpm) spontaneous oscillations in cerebral blood flow are well known and their augmentation after nitric oxide synthase inhibition has recently been described. However, the presence of these oscillations in blood flow velocity in the capillary network of the brain has not been dem...
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Veröffentlicht in: | Microvascular research 1996-07, Vol.52 (1), p.1-12 |
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description | Low-frequency (4–12 cpm) spontaneous oscillations in cerebral blood flow are well known and their augmentation after nitric oxide synthase inhibition has recently been described. However, the presence of these oscillations in blood flow velocity in the capillary network of the brain has not been demonstrated. In this paper, low-frequency oscillations in red blood cell flow velocity in cortical capillaries using intravital video microscopy were studied before and after infusion of the nitric oxide synthase inhibitor Nω-nitro-L-arginine methyl ester (L-NAME). Fluorescein isothiocyanate-labeled red blood cells were injected intravenously and served as markers of capillary flow. Red cell velocity was measured by off-line image tracking. After infusion ofL-NAME (30 mg/kg), red cell velocity decreased from an average of 1.0 ± 0.1 mm/sec to 0.53 ± 0.1 mm/sec. Simultaneously, low-frequency oscillations in velocity emerged as indicated by an 81 ± 17% increase in standard deviation of the 4- to 8-cpm frequency components. There was a significant temporal correlation (r= 0.58,P< 0.01) in red cell velocity between neighboring capillaries afterL-NAME. Principal component analysis suggested that the high temporal correlation was a consequence of low-frequency oscillations rather than phase. These results are consistent with a model in which low-frequency spontaneous oscillations in flow velocity are brought about by precapillary vasomotion. This study provides for the first time direct evidence for low-frequency synchronous oscillations of red cell flow velocity in the cerebral capillary network. |
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However, the presence of these oscillations in blood flow velocity in the capillary network of the brain has not been demonstrated. In this paper, low-frequency oscillations in red blood cell flow velocity in cortical capillaries using intravital video microscopy were studied before and after infusion of the nitric oxide synthase inhibitor Nω-nitro-L-arginine methyl ester (L-NAME). Fluorescein isothiocyanate-labeled red blood cells were injected intravenously and served as markers of capillary flow. Red cell velocity was measured by off-line image tracking. After infusion ofL-NAME (30 mg/kg), red cell velocity decreased from an average of 1.0 ± 0.1 mm/sec to 0.53 ± 0.1 mm/sec. Simultaneously, low-frequency oscillations in velocity emerged as indicated by an 81 ± 17% increase in standard deviation of the 4- to 8-cpm frequency components. There was a significant temporal correlation (r= 0.58,P< 0.01) in red cell velocity between neighboring capillaries afterL-NAME. Principal component analysis suggested that the high temporal correlation was a consequence of low-frequency oscillations rather than phase. These results are consistent with a model in which low-frequency spontaneous oscillations in flow velocity are brought about by precapillary vasomotion. This study provides for the first time direct evidence for low-frequency synchronous oscillations of red cell flow velocity in the cerebral capillary network.</description><identifier>ISSN: 0026-2862</identifier><identifier>EISSN: 1095-9319</identifier><identifier>DOI: 10.1006/mvre.1996.0039</identifier><identifier>PMID: 8812747</identifier><identifier>CODEN: MIVRA6</identifier><language>eng</language><publisher>Amsterdam: Elsevier Inc</publisher><subject>Animals ; Biological and medical sciences ; Biological Clocks - drug effects ; Blood Flow Velocity ; Capillaries ; Cerebral circulation. Blood-brain barrier. Choroid plexus. Cerebrospinal fluid. Circumventricular organ. Meninges ; Cerebrovascular Circulation - physiology ; Enzyme Inhibitors - pharmacology ; Erythrocytes - cytology ; Fundamental and applied biological sciences. Psychology ; Male ; NG-Nitroarginine Methyl Ester - pharmacology ; Nitric Oxide Synthase - antagonists & inhibitors ; Rats ; Rats, Sprague-Dawley ; Vertebrates: nervous system and sense organs</subject><ispartof>Microvascular research, 1996-07, Vol.52 (1), p.1-12</ispartof><rights>1996 Academic Press</rights><rights>1996 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c434t-cc3c62e51313d0c2429b24c7e2415f673700dcc6c5f96ecf0911d1189eceb4d53</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0026286296900394$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3165943$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8812747$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Biswal, Bharat B.</creatorcontrib><creatorcontrib>Hudetz, Antal G.</creatorcontrib><title>Synchronous Oscillations in Cerebrocortical Capillary Red Blood Cell Velocity after Nitric Oxide Synthase Inhibition</title><title>Microvascular research</title><addtitle>Microvasc Res</addtitle><description>Low-frequency (4–12 cpm) spontaneous oscillations in cerebral blood flow are well known and their augmentation after nitric oxide synthase inhibition has recently been described. However, the presence of these oscillations in blood flow velocity in the capillary network of the brain has not been demonstrated. In this paper, low-frequency oscillations in red blood cell flow velocity in cortical capillaries using intravital video microscopy were studied before and after infusion of the nitric oxide synthase inhibitor Nω-nitro-L-arginine methyl ester (L-NAME). Fluorescein isothiocyanate-labeled red blood cells were injected intravenously and served as markers of capillary flow. Red cell velocity was measured by off-line image tracking. After infusion ofL-NAME (30 mg/kg), red cell velocity decreased from an average of 1.0 ± 0.1 mm/sec to 0.53 ± 0.1 mm/sec. Simultaneously, low-frequency oscillations in velocity emerged as indicated by an 81 ± 17% increase in standard deviation of the 4- to 8-cpm frequency components. There was a significant temporal correlation (r= 0.58,P< 0.01) in red cell velocity between neighboring capillaries afterL-NAME. Principal component analysis suggested that the high temporal correlation was a consequence of low-frequency oscillations rather than phase. These results are consistent with a model in which low-frequency spontaneous oscillations in flow velocity are brought about by precapillary vasomotion. This study provides for the first time direct evidence for low-frequency synchronous oscillations of red cell flow velocity in the cerebral capillary network.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Biological Clocks - drug effects</subject><subject>Blood Flow Velocity</subject><subject>Capillaries</subject><subject>Cerebral circulation. Blood-brain barrier. Choroid plexus. Cerebrospinal fluid. Circumventricular organ. Meninges</subject><subject>Cerebrovascular Circulation - physiology</subject><subject>Enzyme Inhibitors - pharmacology</subject><subject>Erythrocytes - cytology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Male</subject><subject>NG-Nitroarginine Methyl Ester - pharmacology</subject><subject>Nitric Oxide Synthase - antagonists & inhibitors</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0026-2862</issn><issn>1095-9319</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kT1v2zAQhomgReImXbMV4FBkk8sPiRLH1kiaAEENtGlXgjqeYBay6JB0UP_7UrCRLROHe-69u4eEXHO25IypL9uXiEuutVoyJvUZWXCmm0pLrt-RBWNCVaJT4oJ8SOkvY5w3WpyT867joq3bBcm_DhNsYpjCPtF1Aj-ONvswJeonusKIfQwQYvZgR7qyu7keD_QnOvptDMEVZhzpHxwD-HygdsgY6Q-fowe6_ucd0jIgb2xC-jBtfO_n8CvyfrBjwo-n95L8vrt9Wt1Xj-vvD6uvjxXUss4VgAQlsOGSS8dA1EL3ooYWRc2bQbWyZcwBKGgGrRAGpjl3nHcaAfvaNfKS3BxzdzE87zFls_UJysJ2wnKvaTupdBlVwOURhBhSijiYXfTbcqjhzMyazazZzJrNrLk0fDol7_stulf85LXUP5_qNhVzQ7QT-PSKSa6aMrhg3RHDYuHFYzTlB3ACdD4iZOOCf2uD_y4amrM</recordid><startdate>19960701</startdate><enddate>19960701</enddate><creator>Biswal, Bharat B.</creator><creator>Hudetz, Antal G.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19960701</creationdate><title>Synchronous Oscillations in Cerebrocortical Capillary Red Blood Cell Velocity after Nitric Oxide Synthase Inhibition</title><author>Biswal, Bharat B. ; Hudetz, Antal G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c434t-cc3c62e51313d0c2429b24c7e2415f673700dcc6c5f96ecf0911d1189eceb4d53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Biological Clocks - drug effects</topic><topic>Blood Flow Velocity</topic><topic>Capillaries</topic><topic>Cerebral circulation. Blood-brain barrier. Choroid plexus. Cerebrospinal fluid. Circumventricular organ. Meninges</topic><topic>Cerebrovascular Circulation - physiology</topic><topic>Enzyme Inhibitors - pharmacology</topic><topic>Erythrocytes - cytology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Male</topic><topic>NG-Nitroarginine Methyl Ester - pharmacology</topic><topic>Nitric Oxide Synthase - antagonists & inhibitors</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Biswal, Bharat B.</creatorcontrib><creatorcontrib>Hudetz, Antal G.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Microvascular research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Biswal, Bharat B.</au><au>Hudetz, Antal G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Synchronous Oscillations in Cerebrocortical Capillary Red Blood Cell Velocity after Nitric Oxide Synthase Inhibition</atitle><jtitle>Microvascular research</jtitle><addtitle>Microvasc Res</addtitle><date>1996-07-01</date><risdate>1996</risdate><volume>52</volume><issue>1</issue><spage>1</spage><epage>12</epage><pages>1-12</pages><issn>0026-2862</issn><eissn>1095-9319</eissn><coden>MIVRA6</coden><abstract>Low-frequency (4–12 cpm) spontaneous oscillations in cerebral blood flow are well known and their augmentation after nitric oxide synthase inhibition has recently been described. However, the presence of these oscillations in blood flow velocity in the capillary network of the brain has not been demonstrated. In this paper, low-frequency oscillations in red blood cell flow velocity in cortical capillaries using intravital video microscopy were studied before and after infusion of the nitric oxide synthase inhibitor Nω-nitro-L-arginine methyl ester (L-NAME). Fluorescein isothiocyanate-labeled red blood cells were injected intravenously and served as markers of capillary flow. Red cell velocity was measured by off-line image tracking. After infusion ofL-NAME (30 mg/kg), red cell velocity decreased from an average of 1.0 ± 0.1 mm/sec to 0.53 ± 0.1 mm/sec. Simultaneously, low-frequency oscillations in velocity emerged as indicated by an 81 ± 17% increase in standard deviation of the 4- to 8-cpm frequency components. There was a significant temporal correlation (r= 0.58,P< 0.01) in red cell velocity between neighboring capillaries afterL-NAME. Principal component analysis suggested that the high temporal correlation was a consequence of low-frequency oscillations rather than phase. These results are consistent with a model in which low-frequency spontaneous oscillations in flow velocity are brought about by precapillary vasomotion. This study provides for the first time direct evidence for low-frequency synchronous oscillations of red cell flow velocity in the cerebral capillary network.</abstract><cop>Amsterdam</cop><pub>Elsevier Inc</pub><pmid>8812747</pmid><doi>10.1006/mvre.1996.0039</doi><tpages>12</tpages></addata></record> |
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subjects | Animals Biological and medical sciences Biological Clocks - drug effects Blood Flow Velocity Capillaries Cerebral circulation. Blood-brain barrier. Choroid plexus. Cerebrospinal fluid. Circumventricular organ. Meninges Cerebrovascular Circulation - physiology Enzyme Inhibitors - pharmacology Erythrocytes - cytology Fundamental and applied biological sciences. Psychology Male NG-Nitroarginine Methyl Ester - pharmacology Nitric Oxide Synthase - antagonists & inhibitors Rats Rats, Sprague-Dawley Vertebrates: nervous system and sense organs |
title | Synchronous Oscillations in Cerebrocortical Capillary Red Blood Cell Velocity after Nitric Oxide Synthase Inhibition |
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