Insulin-Like Growth Factor I Is a Growth and Survival Factor in Human Multiple Myeloma Cell Lines
Human multiple myeloma (MM) represents a highly aneuploid tumor as shown by cytogenetic studies. This may partly explain the heterogeneity with regard to growth factor requirements demonstrated among MM cells. We have previously reported the expression of insulin-like growth factor I (IGF-I) and IGF...
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| Veröffentlicht in: | Blood 1996-09, Vol.88 (6), p.2250-2258 |
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| creator | Georgii-Hemming, Patrik Wiklund, Helena Jernberg Ljunggren, östen Nilsson, Kenneth |
| description | Human multiple myeloma (MM) represents a highly aneuploid tumor as shown by cytogenetic studies. This may partly explain the heterogeneity with regard to growth factor requirements demonstrated among MM cells. We have previously reported the expression of insulin-like growth factor I (IGF-I) and IGF-I receptor (IGF-IR) mRNA in some MM cell lines. In this study we investigated the role of IGF-I as a growth and/or survival factor in three MM cell lines: LP-1, EJM, and Karpas 707. We report that all cell lines expressed IGF-I and IGF-IR mRNA and protein. LP-1 and Karpas 707, but not EJM, were stimulated to proliferation in a dose-dependent manner by exogenous IGF-I. An IGF-IR blocking antibody inhibited both the IGF-l-induced and spontaneous growth of LP-1 and Karpas 707, while the EJM cell line was unaffected by the addition of the antibody. In conclusion, our results show that IGF-I can act as a growth factor in human MM, and they suggest that an autocrine IGF-I loop may contribute to the growth and survival in some MM cell lines. |
| doi_str_mv | 10.1182/blood.V88.6.2250.bloodjournal8862250 |
| format | Article |
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This may partly explain the heterogeneity with regard to growth factor requirements demonstrated among MM cells. We have previously reported the expression of insulin-like growth factor I (IGF-I) and IGF-I receptor (IGF-IR) mRNA in some MM cell lines. In this study we investigated the role of IGF-I as a growth and/or survival factor in three MM cell lines: LP-1, EJM, and Karpas 707. We report that all cell lines expressed IGF-I and IGF-IR mRNA and protein. LP-1 and Karpas 707, but not EJM, were stimulated to proliferation in a dose-dependent manner by exogenous IGF-I. An IGF-IR blocking antibody inhibited both the IGF-l-induced and spontaneous growth of LP-1 and Karpas 707, while the EJM cell line was unaffected by the addition of the antibody. In conclusion, our results show that IGF-I can act as a growth factor in human MM, and they suggest that an autocrine IGF-I loop may contribute to the growth and survival in some MM cell lines.</description><identifier>ISSN: 0006-4971</identifier><identifier>EISSN: 1528-0020</identifier><identifier>DOI: 10.1182/blood.V88.6.2250.bloodjournal8862250</identifier><identifier>PMID: 8822946</identifier><language>eng</language><publisher>Washington, DC: Elsevier Inc</publisher><subject>Base Sequence ; Biological and medical sciences ; Cell Survival ; DNA Primers - chemistry ; Growth Substances - physiology ; Humans ; Immunodeficiencies. Immunoglobulinopathies ; Immunoglobulinopathies ; Immunopathology ; Insulin-Like Growth Factor I - physiology ; Medical sciences ; Molecular Sequence Data ; Multiple Myeloma - pathology ; Receptor, IGF Type 1 - metabolism ; RNA, Messenger - genetics ; RNA, Neoplasm - genetics ; Tumor Cells, Cultured</subject><ispartof>Blood, 1996-09, Vol.88 (6), p.2250-2258</ispartof><rights>1996 American Society of Hematology</rights><rights>1996 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c562t-debec46b5cf96c52d7a60507dc28e5883bc26d63f5d7a19e25e2409038e805f63</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3245111$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8822946$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Georgii-Hemming, Patrik</creatorcontrib><creatorcontrib>Wiklund, Helena Jernberg</creatorcontrib><creatorcontrib>Ljunggren, östen</creatorcontrib><creatorcontrib>Nilsson, Kenneth</creatorcontrib><title>Insulin-Like Growth Factor I Is a Growth and Survival Factor in Human Multiple Myeloma Cell Lines</title><title>Blood</title><addtitle>Blood</addtitle><description>Human multiple myeloma (MM) represents a highly aneuploid tumor as shown by cytogenetic studies. This may partly explain the heterogeneity with regard to growth factor requirements demonstrated among MM cells. We have previously reported the expression of insulin-like growth factor I (IGF-I) and IGF-I receptor (IGF-IR) mRNA in some MM cell lines. In this study we investigated the role of IGF-I as a growth and/or survival factor in three MM cell lines: LP-1, EJM, and Karpas 707. We report that all cell lines expressed IGF-I and IGF-IR mRNA and protein. LP-1 and Karpas 707, but not EJM, were stimulated to proliferation in a dose-dependent manner by exogenous IGF-I. An IGF-IR blocking antibody inhibited both the IGF-l-induced and spontaneous growth of LP-1 and Karpas 707, while the EJM cell line was unaffected by the addition of the antibody. In conclusion, our results show that IGF-I can act as a growth factor in human MM, and they suggest that an autocrine IGF-I loop may contribute to the growth and survival in some MM cell lines.</description><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Cell Survival</subject><subject>DNA Primers - chemistry</subject><subject>Growth Substances - physiology</subject><subject>Humans</subject><subject>Immunodeficiencies. Immunoglobulinopathies</subject><subject>Immunoglobulinopathies</subject><subject>Immunopathology</subject><subject>Insulin-Like Growth Factor I - physiology</subject><subject>Medical sciences</subject><subject>Molecular Sequence Data</subject><subject>Multiple Myeloma - pathology</subject><subject>Receptor, IGF Type 1 - metabolism</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Neoplasm - genetics</subject><subject>Tumor Cells, Cultured</subject><issn>0006-4971</issn><issn>1528-0020</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkEtP3DAUhS1ERYfHT0DyArFqUtuJPc6ymhYYaWgXPLaWY9-oBsee2slU_HsyzMCqm66udM7RuUcfQl8oKSmV7GvrY7Tlo5SlKBnjpHwTnuKYgvZSiq12gGaUM1kQwsghmhFCRFE3c_oZHef8RAitK8aP0JGUjDW1mCG9DHn0LhQr9wz4OsW_w298pc0QE17iZcb6XdTB4rsxbdxG-_eEC_hm7HXAt6Mf3NoDvn0BH3uNF-A9XrkA-RR96rTPcLa_J-jh6sf94qZY_bpeLr6tCsMFGwoLLZhatNx0jTCc2bkWhJO5NUwCl7JqDRNWVB2fHNoA48Bq0pBKgiS8E9UJutz1rlP8M0IeVO-ymWboAHHMai4rUYuGTsHvu6BJMecEnVon1-v0oihRW9LqDayaSCuhtlTVP0hPNef7f2Pbg_0o2aOd_Iu9r7PRvks6GJc_YhWrOaXbNT93MZjYbBwklY2DYMC6BGZQNrr_2_UKLtmorw</recordid><startdate>19960915</startdate><enddate>19960915</enddate><creator>Georgii-Hemming, Patrik</creator><creator>Wiklund, Helena Jernberg</creator><creator>Ljunggren, östen</creator><creator>Nilsson, Kenneth</creator><general>Elsevier Inc</general><general>The Americain Society of Hematology</general><scope>6I.</scope><scope>AAFTH</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19960915</creationdate><title>Insulin-Like Growth Factor I Is a Growth and Survival Factor in Human Multiple Myeloma Cell Lines</title><author>Georgii-Hemming, Patrik ; Wiklund, Helena Jernberg ; Ljunggren, östen ; Nilsson, Kenneth</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c562t-debec46b5cf96c52d7a60507dc28e5883bc26d63f5d7a19e25e2409038e805f63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Cell Survival</topic><topic>DNA Primers - chemistry</topic><topic>Growth Substances - physiology</topic><topic>Humans</topic><topic>Immunodeficiencies. Immunoglobulinopathies</topic><topic>Immunoglobulinopathies</topic><topic>Immunopathology</topic><topic>Insulin-Like Growth Factor I - physiology</topic><topic>Medical sciences</topic><topic>Molecular Sequence Data</topic><topic>Multiple Myeloma - pathology</topic><topic>Receptor, IGF Type 1 - metabolism</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Neoplasm - genetics</topic><topic>Tumor Cells, Cultured</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Georgii-Hemming, Patrik</creatorcontrib><creatorcontrib>Wiklund, Helena Jernberg</creatorcontrib><creatorcontrib>Ljunggren, östen</creatorcontrib><creatorcontrib>Nilsson, Kenneth</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Blood</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Georgii-Hemming, Patrik</au><au>Wiklund, Helena Jernberg</au><au>Ljunggren, östen</au><au>Nilsson, Kenneth</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Insulin-Like Growth Factor I Is a Growth and Survival Factor in Human Multiple Myeloma Cell Lines</atitle><jtitle>Blood</jtitle><addtitle>Blood</addtitle><date>1996-09-15</date><risdate>1996</risdate><volume>88</volume><issue>6</issue><spage>2250</spage><epage>2258</epage><pages>2250-2258</pages><issn>0006-4971</issn><eissn>1528-0020</eissn><abstract>Human multiple myeloma (MM) represents a highly aneuploid tumor as shown by cytogenetic studies. This may partly explain the heterogeneity with regard to growth factor requirements demonstrated among MM cells. We have previously reported the expression of insulin-like growth factor I (IGF-I) and IGF-I receptor (IGF-IR) mRNA in some MM cell lines. In this study we investigated the role of IGF-I as a growth and/or survival factor in three MM cell lines: LP-1, EJM, and Karpas 707. We report that all cell lines expressed IGF-I and IGF-IR mRNA and protein. LP-1 and Karpas 707, but not EJM, were stimulated to proliferation in a dose-dependent manner by exogenous IGF-I. An IGF-IR blocking antibody inhibited both the IGF-l-induced and spontaneous growth of LP-1 and Karpas 707, while the EJM cell line was unaffected by the addition of the antibody. In conclusion, our results show that IGF-I can act as a growth factor in human MM, and they suggest that an autocrine IGF-I loop may contribute to the growth and survival in some MM cell lines.</abstract><cop>Washington, DC</cop><pub>Elsevier Inc</pub><pmid>8822946</pmid><doi>10.1182/blood.V88.6.2250.bloodjournal8862250</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
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| ispartof | Blood, 1996-09, Vol.88 (6), p.2250-2258 |
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| language | eng |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Base Sequence Biological and medical sciences Cell Survival DNA Primers - chemistry Growth Substances - physiology Humans Immunodeficiencies. Immunoglobulinopathies Immunoglobulinopathies Immunopathology Insulin-Like Growth Factor I - physiology Medical sciences Molecular Sequence Data Multiple Myeloma - pathology Receptor, IGF Type 1 - metabolism RNA, Messenger - genetics RNA, Neoplasm - genetics Tumor Cells, Cultured |
| title | Insulin-Like Growth Factor I Is a Growth and Survival Factor in Human Multiple Myeloma Cell Lines |
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