Effects of lipid supplementation of culture media on cell growth, antibody production, membrane structure and dynamics in two hybridomas

Membrane lipid organization and membrane fluidity affect cell functions. The effects of supplementing culture media with a lipid mixture (Ex-Cyte ®) containing cholesterol, phospholipids and fatty acids on cell growth, antibody production and membrane composition and dynamics in two hybridoma cell l...

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Veröffentlicht in:	Journal of biotechnology 1996-07, Vol.48 (1), p.161-173
Hauptverfasser:	Savonnière, Sandrine, Zeghari, Nezha, Miccoli, Laurent, Muller, Sylvaine, Maugras, michel, Donner, Mireille
Format:	Artikel
Sprache:	eng
Schlagworte:	Animal cells Animals Antibodies, Monoclonal - biosynthesis Antibody production Biological and medical sciences Biotechnology Cell Division - drug effects Cell growth Cell hybridization, cell fusion, hybridomas Cell Membrane - drug effects Cell Membrane - metabolism Cell Survival - drug effects Cholesterol - metabolism Culture Media Eukaryotic cell cultures Fundamental and applied biological sciences. Psychology Hybridoma Hybridomas - cytology Hybridomas - immunology Hybridomas - metabolism Lipid supplement Lipids - pharmacology Membrane fluidity Membrane Fluidity - drug effects Membrane Lipids - metabolism Membrane phospholipid composition Methods. Procedures. Technologies Mice Phospholipids - metabolism
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