Effects of lipid supplementation of culture media on cell growth, antibody production, membrane structure and dynamics in two hybridomas
Membrane lipid organization and membrane fluidity affect cell functions. The effects of supplementing culture media with a lipid mixture (Ex-Cyte ®) containing cholesterol, phospholipids and fatty acids on cell growth, antibody production and membrane composition and dynamics in two hybridoma cell l...
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| Veröffentlicht in: | Journal of biotechnology 1996-07, Vol.48 (1), p.161-173 |
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| container_title | Journal of biotechnology |
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| creator | Savonnière, Sandrine Zeghari, Nezha Miccoli, Laurent Muller, Sylvaine Maugras, michel Donner, Mireille |
| description | Membrane lipid organization and membrane fluidity affect cell functions. The effects of supplementing culture media with a lipid mixture (Ex-Cyte
®) containing cholesterol, phospholipids and fatty acids on cell growth, antibody production and membrane composition and dynamics in two hybridoma cell lines were studied. A49 cells decreased immunoglobulin production but cell growth increased. Lipids had no effect on the cell growth rate of B9 cells but increased immunoglobulin production and productivity. The fluidity of the deep areas of the plasma membrane and cytoplasmic organelles increased in the two cell lines. There was increased fluidity of the polar regions of the plasma membrane and a decreased phosphatidylethanolamine/phosphatidylcholine ratio in A49 cells. B9 cells underwent no change in fluidity of the polar regions but the phosphatidylinositol content was increased, together with higher monoclonal antibody production. These results demonstrate that antibody production is not linked to the dynamic properties of the membrane, even though changes in the membrane phosphatidylinositol content are associated with the final step of antibody secretion, but that the action of phospholipids and fatty acids on cell growth is membrane-associated. |
| doi_str_mv | 10.1016/0168-1656(96)01392-2 |
| format | Article |
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®) containing cholesterol, phospholipids and fatty acids on cell growth, antibody production and membrane composition and dynamics in two hybridoma cell lines were studied. A49 cells decreased immunoglobulin production but cell growth increased. Lipids had no effect on the cell growth rate of B9 cells but increased immunoglobulin production and productivity. The fluidity of the deep areas of the plasma membrane and cytoplasmic organelles increased in the two cell lines. There was increased fluidity of the polar regions of the plasma membrane and a decreased phosphatidylethanolamine/phosphatidylcholine ratio in A49 cells. B9 cells underwent no change in fluidity of the polar regions but the phosphatidylinositol content was increased, together with higher monoclonal antibody production. These results demonstrate that antibody production is not linked to the dynamic properties of the membrane, even though changes in the membrane phosphatidylinositol content are associated with the final step of antibody secretion, but that the action of phospholipids and fatty acids on cell growth is membrane-associated.</description><identifier>ISSN: 0168-1656</identifier><identifier>EISSN: 1873-4863</identifier><identifier>DOI: 10.1016/0168-1656(96)01392-2</identifier><identifier>PMID: 8818281</identifier><identifier>CODEN: JBITD4</identifier><language>eng</language><publisher>Lausanne: Elsevier B.V</publisher><subject>Animal cells ; Animals ; Antibodies, Monoclonal - biosynthesis ; Antibody production ; Biological and medical sciences ; Biotechnology ; Cell Division - drug effects ; Cell growth ; Cell hybridization, cell fusion, hybridomas ; Cell Membrane - drug effects ; Cell Membrane - metabolism ; Cell Survival - drug effects ; Cholesterol - metabolism ; Culture Media ; Eukaryotic cell cultures ; Fundamental and applied biological sciences. Psychology ; Hybridoma ; Hybridomas - cytology ; Hybridomas - immunology ; Hybridomas - metabolism ; Lipid supplement ; Lipids - pharmacology ; Membrane fluidity ; Membrane Fluidity - drug effects ; Membrane Lipids - metabolism ; Membrane phospholipid composition ; Methods. Procedures. Technologies ; Mice ; Phospholipids - metabolism</subject><ispartof>Journal of biotechnology, 1996-07, Vol.48 (1), p.161-173</ispartof><rights>1996</rights><rights>1996 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c416t-cf29e510b76fdeea0ff37329d4faaaf6887adec424fb13acd79d1d62b208cab23</citedby><cites>FETCH-LOGICAL-c416t-cf29e510b76fdeea0ff37329d4faaaf6887adec424fb13acd79d1d62b208cab23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0168-1656(96)01392-2$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3225225$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8818281$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Savonnière, Sandrine</creatorcontrib><creatorcontrib>Zeghari, Nezha</creatorcontrib><creatorcontrib>Miccoli, Laurent</creatorcontrib><creatorcontrib>Muller, Sylvaine</creatorcontrib><creatorcontrib>Maugras, michel</creatorcontrib><creatorcontrib>Donner, Mireille</creatorcontrib><title>Effects of lipid supplementation of culture media on cell growth, antibody production, membrane structure and dynamics in two hybridomas</title><title>Journal of biotechnology</title><addtitle>J Biotechnol</addtitle><description>Membrane lipid organization and membrane fluidity affect cell functions. The effects of supplementing culture media with a lipid mixture (Ex-Cyte
®) containing cholesterol, phospholipids and fatty acids on cell growth, antibody production and membrane composition and dynamics in two hybridoma cell lines were studied. A49 cells decreased immunoglobulin production but cell growth increased. Lipids had no effect on the cell growth rate of B9 cells but increased immunoglobulin production and productivity. The fluidity of the deep areas of the plasma membrane and cytoplasmic organelles increased in the two cell lines. There was increased fluidity of the polar regions of the plasma membrane and a decreased phosphatidylethanolamine/phosphatidylcholine ratio in A49 cells. B9 cells underwent no change in fluidity of the polar regions but the phosphatidylinositol content was increased, together with higher monoclonal antibody production. These results demonstrate that antibody production is not linked to the dynamic properties of the membrane, even though changes in the membrane phosphatidylinositol content are associated with the final step of antibody secretion, but that the action of phospholipids and fatty acids on cell growth is membrane-associated.</description><subject>Animal cells</subject><subject>Animals</subject><subject>Antibodies, Monoclonal - biosynthesis</subject><subject>Antibody production</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Cell Division - drug effects</subject><subject>Cell growth</subject><subject>Cell hybridization, cell fusion, hybridomas</subject><subject>Cell Membrane - drug effects</subject><subject>Cell Membrane - metabolism</subject><subject>Cell Survival - drug effects</subject><subject>Cholesterol - metabolism</subject><subject>Culture Media</subject><subject>Eukaryotic cell cultures</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hybridoma</subject><subject>Hybridomas - cytology</subject><subject>Hybridomas - immunology</subject><subject>Hybridomas - metabolism</subject><subject>Lipid supplement</subject><subject>Lipids - pharmacology</subject><subject>Membrane fluidity</subject><subject>Membrane Fluidity - drug effects</subject><subject>Membrane Lipids - metabolism</subject><subject>Membrane phospholipid composition</subject><subject>Methods. Procedures. Technologies</subject><subject>Mice</subject><subject>Phospholipids - metabolism</subject><issn>0168-1656</issn><issn>1873-4863</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkttqFTEUhoModbf6Bgq5EFHoaA5zSG4KUloVCt7odViTg43MTMYkY9lv4GObsDf7UoUcyFrfWvzJH4ReUPKOEtq_L1M0tO_6N7J_SyiXrGGP0I6KgTet6PljtDshT9F5Sj8IIa3s6Bk6E4IKJugO_b5xzuqccHB48qs3OG3rOtnZLhmyD0tN6G3KW7R4tsYDLjFtpwl_j-Eh319iWLIfg9njNQaz6Vp0WdB5jLBYnHIssVoNi8Fmv8DsdcJ-wfkh4Pv9GL0JM6Rn6ImDKdnnx_0Cfbu9-Xr9qbn78vHz9Ye7Rre0z412TNqOknHonbEWiHN84Eya1gGA64UYwFjdstaNlIM2gzTU9GxkRGgYGb9Arw99i9qfm01ZzT7V-xSxYUtqELwTZfknSDvBuqHt_gckg-SygO0B1DGkFK1Ta_QzxL2iRFVLVfVLVb-UrIdiqaqKXx77b2Nx4FR09LDkXx3zkDRMrjy79umEcca6Mgp2dcBsed1f3kaVtLeLLp7G8gOUCf7vOv4AFtvAbw</recordid><startdate>19960718</startdate><enddate>19960718</enddate><creator>Savonnière, Sandrine</creator><creator>Zeghari, Nezha</creator><creator>Miccoli, Laurent</creator><creator>Muller, Sylvaine</creator><creator>Maugras, michel</creator><creator>Donner, Mireille</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19960718</creationdate><title>Effects of lipid supplementation of culture media on cell growth, antibody production, membrane structure and dynamics in two hybridomas</title><author>Savonnière, Sandrine ; Zeghari, Nezha ; Miccoli, Laurent ; Muller, Sylvaine ; Maugras, michel ; Donner, Mireille</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c416t-cf29e510b76fdeea0ff37329d4faaaf6887adec424fb13acd79d1d62b208cab23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Animal cells</topic><topic>Animals</topic><topic>Antibodies, Monoclonal - biosynthesis</topic><topic>Antibody production</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Cell Division - drug effects</topic><topic>Cell growth</topic><topic>Cell hybridization, cell fusion, hybridomas</topic><topic>Cell Membrane - drug effects</topic><topic>Cell Membrane - metabolism</topic><topic>Cell Survival - drug effects</topic><topic>Cholesterol - metabolism</topic><topic>Culture Media</topic><topic>Eukaryotic cell cultures</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hybridoma</topic><topic>Hybridomas - cytology</topic><topic>Hybridomas - immunology</topic><topic>Hybridomas - metabolism</topic><topic>Lipid supplement</topic><topic>Lipids - pharmacology</topic><topic>Membrane fluidity</topic><topic>Membrane Fluidity - drug effects</topic><topic>Membrane Lipids - metabolism</topic><topic>Membrane phospholipid composition</topic><topic>Methods. Procedures. Technologies</topic><topic>Mice</topic><topic>Phospholipids - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Savonnière, Sandrine</creatorcontrib><creatorcontrib>Zeghari, Nezha</creatorcontrib><creatorcontrib>Miccoli, Laurent</creatorcontrib><creatorcontrib>Muller, Sylvaine</creatorcontrib><creatorcontrib>Maugras, michel</creatorcontrib><creatorcontrib>Donner, Mireille</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Savonnière, Sandrine</au><au>Zeghari, Nezha</au><au>Miccoli, Laurent</au><au>Muller, Sylvaine</au><au>Maugras, michel</au><au>Donner, Mireille</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of lipid supplementation of culture media on cell growth, antibody production, membrane structure and dynamics in two hybridomas</atitle><jtitle>Journal of biotechnology</jtitle><addtitle>J Biotechnol</addtitle><date>1996-07-18</date><risdate>1996</risdate><volume>48</volume><issue>1</issue><spage>161</spage><epage>173</epage><pages>161-173</pages><issn>0168-1656</issn><eissn>1873-4863</eissn><coden>JBITD4</coden><abstract>Membrane lipid organization and membrane fluidity affect cell functions. The effects of supplementing culture media with a lipid mixture (Ex-Cyte
®) containing cholesterol, phospholipids and fatty acids on cell growth, antibody production and membrane composition and dynamics in two hybridoma cell lines were studied. A49 cells decreased immunoglobulin production but cell growth increased. Lipids had no effect on the cell growth rate of B9 cells but increased immunoglobulin production and productivity. The fluidity of the deep areas of the plasma membrane and cytoplasmic organelles increased in the two cell lines. There was increased fluidity of the polar regions of the plasma membrane and a decreased phosphatidylethanolamine/phosphatidylcholine ratio in A49 cells. B9 cells underwent no change in fluidity of the polar regions but the phosphatidylinositol content was increased, together with higher monoclonal antibody production. These results demonstrate that antibody production is not linked to the dynamic properties of the membrane, even though changes in the membrane phosphatidylinositol content are associated with the final step of antibody secretion, but that the action of phospholipids and fatty acids on cell growth is membrane-associated.</abstract><cop>Lausanne</cop><cop>Amsterdam</cop><cop>New York, NY</cop><pub>Elsevier B.V</pub><pmid>8818281</pmid><doi>10.1016/0168-1656(96)01392-2</doi><tpages>13</tpages></addata></record> |
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| identifier | ISSN: 0168-1656 |
| ispartof | Journal of biotechnology, 1996-07, Vol.48 (1), p.161-173 |
| issn | 0168-1656 1873-4863 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Animal cells Animals Antibodies, Monoclonal - biosynthesis Antibody production Biological and medical sciences Biotechnology Cell Division - drug effects Cell growth Cell hybridization, cell fusion, hybridomas Cell Membrane - drug effects Cell Membrane - metabolism Cell Survival - drug effects Cholesterol - metabolism Culture Media Eukaryotic cell cultures Fundamental and applied biological sciences. Psychology Hybridoma Hybridomas - cytology Hybridomas - immunology Hybridomas - metabolism Lipid supplement Lipids - pharmacology Membrane fluidity Membrane Fluidity - drug effects Membrane Lipids - metabolism Membrane phospholipid composition Methods. Procedures. Technologies Mice Phospholipids - metabolism |
| title | Effects of lipid supplementation of culture media on cell growth, antibody production, membrane structure and dynamics in two hybridomas |
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