Ferredoxin and cytochrome P-450scc concentrations in granulosa cells of porcine ovaries during follicular cell growth and luteinization
The concentrations of cytochrome P-450scc and ferredoxin, two of the three proteins which comprise the mitochondrial steroidogenic electron transport chain, were measured in granulosa and luteal cells from porcine ovaries by an immunoblot procedure. During the follicular phase of the ovarian cycle t...
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| Veröffentlicht in: | Journal of steroid biochemistry 1988-08, Vol.31 (2), p.201-205 |
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| container_title | Journal of steroid biochemistry |
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| creator | TUCKEY, R. C KOSTADINOVIC, Z STEVENSON, P. M |
| description | The concentrations of cytochrome P-450scc and ferredoxin, two of the three proteins which comprise the mitochondrial steroidogenic electron transport chain, were measured in granulosa and luteal cells from porcine ovaries by an immunoblot procedure. During the follicular phase of the ovarian cycle the concentration of cytochrome P-450scc increased 5-fold and ferredoxin increased 3-fold. When the large follicles developed into corpora lutea the cytochrome P-450scc concentration increased a further 7-fold while ferredoxin increased only 3-fold. These changes were coincident with an overall 4-fold increase in the concentration of ferredoxin reductase during follicular cell development and luteinization. Analysis of the data revealed that the concentration of ferredoxin, which shuttles electrons from ferredoxin reductase to cytochrome P-450scc, was always adequate to saturate both the reductase and cytochrome P-450scc. This came about from a co-ordinate increase in the concentration of cytochrome P-450scc and the concentration of ferredoxin minus ferredoxin reductase. |
| doi_str_mv | 10.1016/0022-4731(88)90055-6 |
| format | Article |
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C ; KOSTADINOVIC, Z ; STEVENSON, P. M</creator><creatorcontrib>TUCKEY, R. C ; KOSTADINOVIC, Z ; STEVENSON, P. M</creatorcontrib><description>The concentrations of cytochrome P-450scc and ferredoxin, two of the three proteins which comprise the mitochondrial steroidogenic electron transport chain, were measured in granulosa and luteal cells from porcine ovaries by an immunoblot procedure. During the follicular phase of the ovarian cycle the concentration of cytochrome P-450scc increased 5-fold and ferredoxin increased 3-fold. When the large follicles developed into corpora lutea the cytochrome P-450scc concentration increased a further 7-fold while ferredoxin increased only 3-fold. These changes were coincident with an overall 4-fold increase in the concentration of ferredoxin reductase during follicular cell development and luteinization. Analysis of the data revealed that the concentration of ferredoxin, which shuttles electrons from ferredoxin reductase to cytochrome P-450scc, was always adequate to saturate both the reductase and cytochrome P-450scc. This came about from a co-ordinate increase in the concentration of cytochrome P-450scc and the concentration of ferredoxin minus ferredoxin reductase.</description><identifier>ISSN: 0022-4731</identifier><identifier>DOI: 10.1016/0022-4731(88)90055-6</identifier><identifier>PMID: 3404990</identifier><identifier>CODEN: JSTBBK</identifier><language>eng</language><publisher>Oxford: Pergamon</publisher><subject>Animals ; Antibody Specificity ; Biological and medical sciences ; Cholesterol Side-Chain Cleavage Enzyme - immunology ; Cholesterol Side-Chain Cleavage Enzyme - metabolism ; Corpus Luteum - physiology ; Electrophoresis, Polyacrylamide Gel ; Female ; Ferredoxin-NADP Reductase - metabolism ; Ferredoxins - immunology ; Ferredoxins - metabolism ; Fundamental and applied biological sciences. Psychology ; Granulosa Cells - physiology ; Immune Sera - immunology ; Immunoassay ; Luteal Cells - physiology ; Mammalian female genital system ; Morphology. Physiology ; Ovarian Follicle - physiology ; Swine ; Vertebrates: reproduction</subject><ispartof>Journal of steroid biochemistry, 1988-08, Vol.31 (2), p.201-205</ispartof><rights>1989 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c331t-8b38f4ef89ac296ec17d690b231b8483b47ff8463763d2059e24093c996c0a943</citedby><cites>FETCH-LOGICAL-c331t-8b38f4ef89ac296ec17d690b231b8483b47ff8463763d2059e24093c996c0a943</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,782,786,27931,27932</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7056570$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3404990$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>TUCKEY, R. C</creatorcontrib><creatorcontrib>KOSTADINOVIC, Z</creatorcontrib><creatorcontrib>STEVENSON, P. M</creatorcontrib><title>Ferredoxin and cytochrome P-450scc concentrations in granulosa cells of porcine ovaries during follicular cell growth and luteinization</title><title>Journal of steroid biochemistry</title><addtitle>J Steroid Biochem</addtitle><description>The concentrations of cytochrome P-450scc and ferredoxin, two of the three proteins which comprise the mitochondrial steroidogenic electron transport chain, were measured in granulosa and luteal cells from porcine ovaries by an immunoblot procedure. During the follicular phase of the ovarian cycle the concentration of cytochrome P-450scc increased 5-fold and ferredoxin increased 3-fold. When the large follicles developed into corpora lutea the cytochrome P-450scc concentration increased a further 7-fold while ferredoxin increased only 3-fold. These changes were coincident with an overall 4-fold increase in the concentration of ferredoxin reductase during follicular cell development and luteinization. Analysis of the data revealed that the concentration of ferredoxin, which shuttles electrons from ferredoxin reductase to cytochrome P-450scc, was always adequate to saturate both the reductase and cytochrome P-450scc. This came about from a co-ordinate increase in the concentration of cytochrome P-450scc and the concentration of ferredoxin minus ferredoxin reductase.</description><subject>Animals</subject><subject>Antibody Specificity</subject><subject>Biological and medical sciences</subject><subject>Cholesterol Side-Chain Cleavage Enzyme - immunology</subject><subject>Cholesterol Side-Chain Cleavage Enzyme - metabolism</subject><subject>Corpus Luteum - physiology</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Female</subject><subject>Ferredoxin-NADP Reductase - metabolism</subject><subject>Ferredoxins - immunology</subject><subject>Ferredoxins - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Granulosa Cells - physiology</subject><subject>Immune Sera - immunology</subject><subject>Immunoassay</subject><subject>Luteal Cells - physiology</subject><subject>Mammalian female genital system</subject><subject>Morphology. Physiology</subject><subject>Ovarian Follicle - physiology</subject><subject>Swine</subject><subject>Vertebrates: reproduction</subject><issn>0022-4731</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1988</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kMFu1DAQhn2gKm3hDajkQ4XKITCOHcc-oqoFpEpwgLPlTOzWldfe2gmlvACvTbJd7Wkkz_f_Y32EvGPwkQGTnwDathE9Z5dKfdAAXdfIV-Tk8PyanNb6AMC0Eu0xOeYChNZwQv7duFLcmP-ERG0aKT5PGe9L3jj6oxEdVESKOaFLU7FTyKnShbwrNs0xV0vRxVhp9nSbC4bkaP5tS3CVjnMJ6Y76HGPAOdqyQ5dkfprud6fiPLmQwt9d7Rty5G2s7u1-npFfN9c_r742t9-_fLv6fNsg52xq1MCVF84rbbHV0iHrR6lhaDkblFB8EL33SkjeSz620GnXCtActZYIVgt-Rt6_9G5Lfpxdncwm1PVnNrk8V9Mr3nVcyQUULyCWXGtx3mxL2NjybBiY1blZ5ZpVrlHK7JybNXa-75-HjRsPob3wZX-x39uKNvpFJIZ6wHroZNcD_w8gbo1A</recordid><startdate>19880801</startdate><enddate>19880801</enddate><creator>TUCKEY, R. C</creator><creator>KOSTADINOVIC, Z</creator><creator>STEVENSON, P. M</creator><general>Pergamon</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19880801</creationdate><title>Ferredoxin and cytochrome P-450scc concentrations in granulosa cells of porcine ovaries during follicular cell growth and luteinization</title><author>TUCKEY, R. C ; KOSTADINOVIC, Z ; STEVENSON, P. M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c331t-8b38f4ef89ac296ec17d690b231b8483b47ff8463763d2059e24093c996c0a943</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1988</creationdate><topic>Animals</topic><topic>Antibody Specificity</topic><topic>Biological and medical sciences</topic><topic>Cholesterol Side-Chain Cleavage Enzyme - immunology</topic><topic>Cholesterol Side-Chain Cleavage Enzyme - metabolism</topic><topic>Corpus Luteum - physiology</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Female</topic><topic>Ferredoxin-NADP Reductase - metabolism</topic><topic>Ferredoxins - immunology</topic><topic>Ferredoxins - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Granulosa Cells - physiology</topic><topic>Immune Sera - immunology</topic><topic>Immunoassay</topic><topic>Luteal Cells - physiology</topic><topic>Mammalian female genital system</topic><topic>Morphology. Physiology</topic><topic>Ovarian Follicle - physiology</topic><topic>Swine</topic><topic>Vertebrates: reproduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>TUCKEY, R. C</creatorcontrib><creatorcontrib>KOSTADINOVIC, Z</creatorcontrib><creatorcontrib>STEVENSON, P. 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M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Ferredoxin and cytochrome P-450scc concentrations in granulosa cells of porcine ovaries during follicular cell growth and luteinization</atitle><jtitle>Journal of steroid biochemistry</jtitle><addtitle>J Steroid Biochem</addtitle><date>1988-08-01</date><risdate>1988</risdate><volume>31</volume><issue>2</issue><spage>201</spage><epage>205</epage><pages>201-205</pages><issn>0022-4731</issn><coden>JSTBBK</coden><abstract>The concentrations of cytochrome P-450scc and ferredoxin, two of the three proteins which comprise the mitochondrial steroidogenic electron transport chain, were measured in granulosa and luteal cells from porcine ovaries by an immunoblot procedure. During the follicular phase of the ovarian cycle the concentration of cytochrome P-450scc increased 5-fold and ferredoxin increased 3-fold. When the large follicles developed into corpora lutea the cytochrome P-450scc concentration increased a further 7-fold while ferredoxin increased only 3-fold. These changes were coincident with an overall 4-fold increase in the concentration of ferredoxin reductase during follicular cell development and luteinization. Analysis of the data revealed that the concentration of ferredoxin, which shuttles electrons from ferredoxin reductase to cytochrome P-450scc, was always adequate to saturate both the reductase and cytochrome P-450scc. This came about from a co-ordinate increase in the concentration of cytochrome P-450scc and the concentration of ferredoxin minus ferredoxin reductase.</abstract><cop>Oxford</cop><cop>New York, NY</cop><pub>Pergamon</pub><pmid>3404990</pmid><doi>10.1016/0022-4731(88)90055-6</doi><tpages>5</tpages></addata></record> |
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| ispartof | Journal of steroid biochemistry, 1988-08, Vol.31 (2), p.201-205 |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Animals Antibody Specificity Biological and medical sciences Cholesterol Side-Chain Cleavage Enzyme - immunology Cholesterol Side-Chain Cleavage Enzyme - metabolism Corpus Luteum - physiology Electrophoresis, Polyacrylamide Gel Female Ferredoxin-NADP Reductase - metabolism Ferredoxins - immunology Ferredoxins - metabolism Fundamental and applied biological sciences. Psychology Granulosa Cells - physiology Immune Sera - immunology Immunoassay Luteal Cells - physiology Mammalian female genital system Morphology. Physiology Ovarian Follicle - physiology Swine Vertebrates: reproduction |
| title | Ferredoxin and cytochrome P-450scc concentrations in granulosa cells of porcine ovaries during follicular cell growth and luteinization |
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