Polymerase chain reaction for diagnosis of M. tuberculosis: comparison of simple boiling and a conventional method for DNA extraction

Although the polymerase chain reaction (PCR) has been used increasingly for rapid diagnosis of tuberculosis (TB) in clinical specimens, no consensus exists regarding DNA extraction protocols. We compared a simple boiling method to a conventional, labor-intensive chemical method using lysozyme and si...

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Veröffentlicht in:	Biochemical and molecular medicine 1996-02, Vol.57 (1), p.14-18
Hauptverfasser:	Afghani, B, Stutman, H R
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacteriological Techniques DNA, Bacterial - isolation & purification Mycobacterium tuberculosis - genetics Mycobacterium tuberculosis - growth & development Polymerase Chain Reaction - methods Sensitivity and Specificity Tuberculosis - diagnosis
Online-Zugang:	Volltext
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