Influence of Hormone Status on Enzymes Released from Renal Cortical Slices of Wistar Rats
Release of some cytosolic (aspartate aminotransferase, alanine aminotransferase and lactate dehydrogenase) and brush border (γ‐glutamyltransferase and alkaline phosphatase) enzymes from renal cortical slices was studied in vitro. Renal cortical slices were prepared freehand from 3‐month‐old male and...
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| Veröffentlicht in: | Journal of applied toxicology 1996-05, Vol.16 (3), p.255-257 |
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| creator | Maso, Stefano Nicoletto, Giampaolo Secondin, Livia Odinecs, Aleksandrs Trevisan, Andrea |
| description | Release of some cytosolic (aspartate aminotransferase, alanine aminotransferase and lactate dehydrogenase) and brush border (γ‐glutamyltransferase and alkaline phosphatase) enzymes from renal cortical slices was studied in vitro. Renal cortical slices were prepared freehand from 3‐month‐old male and female Wistar rats of different hormonal status. Some male and female rats were castrated at 1 month of age and a portion of castrated males and of naive males and females were s.c. treated with testosterone (10 mg kg−1 body wt.) on alternate days for 3 weeks. Females had higher alanine aminotransferase (77.5 ± 2.8 nmol 100 mg−1 tissue), lactate dehydrogenase (5.01 ± 0.24 μmol) and alkaline phosphatase (1.63 ± 0.15 mol) activities than male rats (20.4 ± 0.9, 3.99 ± 0.19 and 0.91 ± 0.02, respectively). On the contrary, aspartate aminotransferase and γ‐glutamyltransferase were similar. Among cytosolic enzymes, alanine aminotransferase and lactate dehydrogenase appeared to be sexual hormone‐dependent enzymes: castration significantly increased enzyme activities in males (49.6 ± 1.1 for the former; 5.30 ± 0.15 for the latter) and caused significant decreases in females (alanine aminotransferase only 47.1 ± 1.5), whereas testosterone pretreatment decreased activities in cortical slices from female (48.1 ± 3.6 and 3.81 ± 0.07, respectively) and castrated male (27.4 ± 1.8 and 4.05 ± 0.15, respectively). Moreover, exogenous testosterone increased aspartate aminotransferase in males (1.05 ± 0.01 μmol) and castration increased it in both sexes. The activity of brush border enzymes was increased by testosterone pretreatment and decreased by castration (mainly alkaline phosphatase). |
| doi_str_mv | 10.1002/(SICI)1099-1263(199605)16:3<255::AID-JAT341>3.0.CO;2-P |
| format | Article |
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Renal cortical slices were prepared freehand from 3‐month‐old male and female Wistar rats of different hormonal status. Some male and female rats were castrated at 1 month of age and a portion of castrated males and of naive males and females were s.c. treated with testosterone (10 mg kg−1 body wt.) on alternate days for 3 weeks. Females had higher alanine aminotransferase (77.5 ± 2.8 nmol 100 mg−1 tissue), lactate dehydrogenase (5.01 ± 0.24 μmol) and alkaline phosphatase (1.63 ± 0.15 mol) activities than male rats (20.4 ± 0.9, 3.99 ± 0.19 and 0.91 ± 0.02, respectively). On the contrary, aspartate aminotransferase and γ‐glutamyltransferase were similar. Among cytosolic enzymes, alanine aminotransferase and lactate dehydrogenase appeared to be sexual hormone‐dependent enzymes: castration significantly increased enzyme activities in males (49.6 ± 1.1 for the former; 5.30 ± 0.15 for the latter) and caused significant decreases in females (alanine aminotransferase only 47.1 ± 1.5), whereas testosterone pretreatment decreased activities in cortical slices from female (48.1 ± 3.6 and 3.81 ± 0.07, respectively) and castrated male (27.4 ± 1.8 and 4.05 ± 0.15, respectively). Moreover, exogenous testosterone increased aspartate aminotransferase in males (1.05 ± 0.01 μmol) and castration increased it in both sexes. The activity of brush border enzymes was increased by testosterone pretreatment and decreased by castration (mainly alkaline phosphatase).</description><identifier>ISSN: 0260-437X</identifier><identifier>EISSN: 1099-1263</identifier><identifier>DOI: 10.1002/(SICI)1099-1263(199605)16:3<255::AID-JAT341>3.0.CO;2-P</identifier><identifier>PMID: 8818867</identifier><identifier>CODEN: JJATDK</identifier><language>eng</language><publisher>Chichester: John Wiley & Sons, Ltd</publisher><subject>alanine aminotransferase ; Alanine Transaminase - drug effects ; Alanine Transaminase - secretion ; alkaline phosphatase ; Alkaline Phosphatase - drug effects ; Alkaline Phosphatase - secretion ; Animals ; aspartate aminotransferase ; Aspartate Aminotransferases - drug effects ; Aspartate Aminotransferases - secretion ; Biological and medical sciences ; Castration - adverse effects ; Enzymes - drug effects ; Enzymes - secretion ; Female ; gamma-Glutamyltransferase - drug effects ; gamma-Glutamyltransferase - secretion ; General aspects. Methods ; Hormones - analysis ; Hormones - pharmacology ; In Vitro Techniques ; Kidney Cortex - drug effects ; Kidney Cortex - enzymology ; Kidney Tubules, Proximal - drug effects ; Kidney Tubules, Proximal - enzymology ; L-Lactate Dehydrogenase - drug effects ; L-Lactate Dehydrogenase - secretion ; lactate dehydrogenase ; Male ; Medical sciences ; Rats ; Rats, Wistar ; sexual hormones ; Testosterone - analysis ; Testosterone - pharmacology ; Toxicology ; γ-glutamyltransferase</subject><ispartof>Journal of applied toxicology, 1996-05, Vol.16 (3), p.255-257</ispartof><rights>Copyright © 1996 John Wiley & Sons, Ltd.</rights><rights>1996 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2F%28SICI%291099-1263%28199605%2916%3A3%3C255%3A%3AAID-JAT341%3E3.0.CO%3B2-P$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2F%28SICI%291099-1263%28199605%2916%3A3%3C255%3A%3AAID-JAT341%3E3.0.CO%3B2-P$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3166008$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8818867$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Maso, Stefano</creatorcontrib><creatorcontrib>Nicoletto, Giampaolo</creatorcontrib><creatorcontrib>Secondin, Livia</creatorcontrib><creatorcontrib>Odinecs, Aleksandrs</creatorcontrib><creatorcontrib>Trevisan, Andrea</creatorcontrib><title>Influence of Hormone Status on Enzymes Released from Renal Cortical Slices of Wistar Rats</title><title>Journal of applied toxicology</title><addtitle>J. Appl. Toxicol</addtitle><description>Release of some cytosolic (aspartate aminotransferase, alanine aminotransferase and lactate dehydrogenase) and brush border (γ‐glutamyltransferase and alkaline phosphatase) enzymes from renal cortical slices was studied in vitro. Renal cortical slices were prepared freehand from 3‐month‐old male and female Wistar rats of different hormonal status. Some male and female rats were castrated at 1 month of age and a portion of castrated males and of naive males and females were s.c. treated with testosterone (10 mg kg−1 body wt.) on alternate days for 3 weeks. Females had higher alanine aminotransferase (77.5 ± 2.8 nmol 100 mg−1 tissue), lactate dehydrogenase (5.01 ± 0.24 μmol) and alkaline phosphatase (1.63 ± 0.15 mol) activities than male rats (20.4 ± 0.9, 3.99 ± 0.19 and 0.91 ± 0.02, respectively). On the contrary, aspartate aminotransferase and γ‐glutamyltransferase were similar. Among cytosolic enzymes, alanine aminotransferase and lactate dehydrogenase appeared to be sexual hormone‐dependent enzymes: castration significantly increased enzyme activities in males (49.6 ± 1.1 for the former; 5.30 ± 0.15 for the latter) and caused significant decreases in females (alanine aminotransferase only 47.1 ± 1.5), whereas testosterone pretreatment decreased activities in cortical slices from female (48.1 ± 3.6 and 3.81 ± 0.07, respectively) and castrated male (27.4 ± 1.8 and 4.05 ± 0.15, respectively). Moreover, exogenous testosterone increased aspartate aminotransferase in males (1.05 ± 0.01 μmol) and castration increased it in both sexes. The activity of brush border enzymes was increased by testosterone pretreatment and decreased by castration (mainly alkaline phosphatase).</description><subject>alanine aminotransferase</subject><subject>Alanine Transaminase - drug effects</subject><subject>Alanine Transaminase - secretion</subject><subject>alkaline phosphatase</subject><subject>Alkaline Phosphatase - drug effects</subject><subject>Alkaline Phosphatase - secretion</subject><subject>Animals</subject><subject>aspartate aminotransferase</subject><subject>Aspartate Aminotransferases - drug effects</subject><subject>Aspartate Aminotransferases - secretion</subject><subject>Biological and medical sciences</subject><subject>Castration - adverse effects</subject><subject>Enzymes - drug effects</subject><subject>Enzymes - secretion</subject><subject>Female</subject><subject>gamma-Glutamyltransferase - drug effects</subject><subject>gamma-Glutamyltransferase - secretion</subject><subject>General aspects. Methods</subject><subject>Hormones - analysis</subject><subject>Hormones - pharmacology</subject><subject>In Vitro Techniques</subject><subject>Kidney Cortex - drug effects</subject><subject>Kidney Cortex - enzymology</subject><subject>Kidney Tubules, Proximal - drug effects</subject><subject>Kidney Tubules, Proximal - enzymology</subject><subject>L-Lactate Dehydrogenase - drug effects</subject><subject>L-Lactate Dehydrogenase - secretion</subject><subject>lactate dehydrogenase</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>sexual hormones</subject><subject>Testosterone - analysis</subject><subject>Testosterone - pharmacology</subject><subject>Toxicology</subject><subject>γ-glutamyltransferase</subject><issn>0260-437X</issn><issn>1099-1263</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkV1v0zAUhi3ENMrgJyDlAqHtIsUfsZOUaVLJxhao6LQWOrg5chxHCsvHZica5dfjKlFvQNqV7XNev3rPeRA6I3hKMKbvj1dpkp4QHMc-oYIdkzgWmJ8QMWOnlPPZbJ6e-5_naxaQMzbF02T5gfrXz9Bk_-U5mmAqsB-w8PYFemntL4xdj0aH6DCKSBSJcIJ-pE1R9bpR2msL76o1ddtob9XJrrde23gXzZ9tra13oystrc69wrS1ezWy8pLWdKVyl1VVKqdxBpvSdtJ4N7Kzr9BBISurX4_nEfr26WKdXPmL5WWazBe-4gElPsVZHuNcZioWhc4Jl5nIiCS5IrkUUhKsiWJMZyqLQ8kFiwKhiiATOMaK65wdoXeD771pH3ptO6hLq3RVyUa3vYUwYkEQ8vBJIeEhZyGjTvh9ECrTWmt0AfemrKXZAsGwgwOwgwO7TcNu0zDAASKAgYMD4ODAAMdVMCRLoHDtjN-MCfqs1vnedqTh-m_HvrRur4WRjSrtXsaIEBhHTnY7yB7LSm__CfdEtv9GGyvO2h-sHUf9e28tzR24fCGHzddLN8gi3nxcf4Gf7C8IQsbZ</recordid><startdate>199605</startdate><enddate>199605</enddate><creator>Maso, Stefano</creator><creator>Nicoletto, Giampaolo</creator><creator>Secondin, Livia</creator><creator>Odinecs, Aleksandrs</creator><creator>Trevisan, Andrea</creator><general>John Wiley & Sons, Ltd</general><general>Wiley</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U7</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>199605</creationdate><title>Influence of Hormone Status on Enzymes Released from Renal Cortical Slices of Wistar Rats</title><author>Maso, Stefano ; Nicoletto, Giampaolo ; Secondin, Livia ; Odinecs, Aleksandrs ; Trevisan, Andrea</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5421-20bd90dabc96fed15ab6b1a1dc1da6aa10e1c33ebcb97a563846cf4b6090c5ed3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>alanine aminotransferase</topic><topic>Alanine Transaminase - drug effects</topic><topic>Alanine Transaminase - secretion</topic><topic>alkaline phosphatase</topic><topic>Alkaline Phosphatase - drug effects</topic><topic>Alkaline Phosphatase - secretion</topic><topic>Animals</topic><topic>aspartate aminotransferase</topic><topic>Aspartate Aminotransferases - drug effects</topic><topic>Aspartate Aminotransferases - secretion</topic><topic>Biological and medical sciences</topic><topic>Castration - adverse effects</topic><topic>Enzymes - drug effects</topic><topic>Enzymes - secretion</topic><topic>Female</topic><topic>gamma-Glutamyltransferase - drug effects</topic><topic>gamma-Glutamyltransferase - secretion</topic><topic>General aspects. Methods</topic><topic>Hormones - analysis</topic><topic>Hormones - pharmacology</topic><topic>In Vitro Techniques</topic><topic>Kidney Cortex - drug effects</topic><topic>Kidney Cortex - enzymology</topic><topic>Kidney Tubules, Proximal - drug effects</topic><topic>Kidney Tubules, Proximal - enzymology</topic><topic>L-Lactate Dehydrogenase - drug effects</topic><topic>L-Lactate Dehydrogenase - secretion</topic><topic>lactate dehydrogenase</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>sexual hormones</topic><topic>Testosterone - analysis</topic><topic>Testosterone - pharmacology</topic><topic>Toxicology</topic><topic>γ-glutamyltransferase</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Maso, Stefano</creatorcontrib><creatorcontrib>Nicoletto, Giampaolo</creatorcontrib><creatorcontrib>Secondin, Livia</creatorcontrib><creatorcontrib>Odinecs, Aleksandrs</creatorcontrib><creatorcontrib>Trevisan, Andrea</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of applied toxicology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Maso, Stefano</au><au>Nicoletto, Giampaolo</au><au>Secondin, Livia</au><au>Odinecs, Aleksandrs</au><au>Trevisan, Andrea</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Influence of Hormone Status on Enzymes Released from Renal Cortical Slices of Wistar Rats</atitle><jtitle>Journal of applied toxicology</jtitle><addtitle>J. Appl. Toxicol</addtitle><date>1996-05</date><risdate>1996</risdate><volume>16</volume><issue>3</issue><spage>255</spage><epage>257</epage><pages>255-257</pages><issn>0260-437X</issn><eissn>1099-1263</eissn><coden>JJATDK</coden><abstract>Release of some cytosolic (aspartate aminotransferase, alanine aminotransferase and lactate dehydrogenase) and brush border (γ‐glutamyltransferase and alkaline phosphatase) enzymes from renal cortical slices was studied in vitro. Renal cortical slices were prepared freehand from 3‐month‐old male and female Wistar rats of different hormonal status. Some male and female rats were castrated at 1 month of age and a portion of castrated males and of naive males and females were s.c. treated with testosterone (10 mg kg−1 body wt.) on alternate days for 3 weeks. Females had higher alanine aminotransferase (77.5 ± 2.8 nmol 100 mg−1 tissue), lactate dehydrogenase (5.01 ± 0.24 μmol) and alkaline phosphatase (1.63 ± 0.15 mol) activities than male rats (20.4 ± 0.9, 3.99 ± 0.19 and 0.91 ± 0.02, respectively). On the contrary, aspartate aminotransferase and γ‐glutamyltransferase were similar. Among cytosolic enzymes, alanine aminotransferase and lactate dehydrogenase appeared to be sexual hormone‐dependent enzymes: castration significantly increased enzyme activities in males (49.6 ± 1.1 for the former; 5.30 ± 0.15 for the latter) and caused significant decreases in females (alanine aminotransferase only 47.1 ± 1.5), whereas testosterone pretreatment decreased activities in cortical slices from female (48.1 ± 3.6 and 3.81 ± 0.07, respectively) and castrated male (27.4 ± 1.8 and 4.05 ± 0.15, respectively). Moreover, exogenous testosterone increased aspartate aminotransferase in males (1.05 ± 0.01 μmol) and castration increased it in both sexes. The activity of brush border enzymes was increased by testosterone pretreatment and decreased by castration (mainly alkaline phosphatase).</abstract><cop>Chichester</cop><pub>John Wiley & Sons, Ltd</pub><pmid>8818867</pmid><doi>10.1002/(SICI)1099-1263(199605)16:3<255::AID-JAT341>3.0.CO;2-P</doi><tpages>3</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of applied toxicology, 1996-05, Vol.16 (3), p.255-257 |
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| source | Wiley Online Library - AutoHoldings Journals; MEDLINE |
| subjects | alanine aminotransferase Alanine Transaminase - drug effects Alanine Transaminase - secretion alkaline phosphatase Alkaline Phosphatase - drug effects Alkaline Phosphatase - secretion Animals aspartate aminotransferase Aspartate Aminotransferases - drug effects Aspartate Aminotransferases - secretion Biological and medical sciences Castration - adverse effects Enzymes - drug effects Enzymes - secretion Female gamma-Glutamyltransferase - drug effects gamma-Glutamyltransferase - secretion General aspects. Methods Hormones - analysis Hormones - pharmacology In Vitro Techniques Kidney Cortex - drug effects Kidney Cortex - enzymology Kidney Tubules, Proximal - drug effects Kidney Tubules, Proximal - enzymology L-Lactate Dehydrogenase - drug effects L-Lactate Dehydrogenase - secretion lactate dehydrogenase Male Medical sciences Rats Rats, Wistar sexual hormones Testosterone - analysis Testosterone - pharmacology Toxicology γ-glutamyltransferase |
| title | Influence of Hormone Status on Enzymes Released from Renal Cortical Slices of Wistar Rats |
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