Rapid purification of native C protein from nuclear ribonucleoprotein particles

A rapid three step procedure is described for the purification of C protein from HeLa 40 S hnRNP particles. The procedure takes advantage of the salt resistant RNA binding of C protein, the size of the C protein-RNA complex, and the strong binding of C protein to an anion-exchange resin. Typically 1...

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Veröffentlicht in:	Journal of biochemical and biophysical methods 1988-05, Vol.16 (1), p.87-98
Hauptverfasser:	Barnett, S F, LeStourgeon, W M, Friedman, D L
Format:	Artikel
Sprache:	eng
Schlagworte:	Carrier Proteins - isolation & purification Carrier Proteins - metabolism Cell Nucleus - analysis Chromatography, Gel Chromatography, Ion Exchange Electrophoresis, Polyacrylamide Gel HeLa Cells Humans ribonucleoproteins Ribonucleoproteins - analysis RNA - metabolism Sodium Chloride
Online-Zugang:	Volltext
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