Expressions of Very Late Antigen-6 and Vitronectin Receptor, and Their Interactions to Laminin and Vitronectin during Tonsillar B-Cell Activation
This study examined the expressions of a-subunits of very late antigen-6 (VLA-6; a6) and vitronectin receptor (VNR; αV) on tonsillar B cells and interactions between those integrins and their respective ligands, laminin (LM) and vitronectin (VN). α6 and αV were expressed on about 30 to 40% of tonsil...
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| Veröffentlicht in: | Auris, nasus, larynx nasus, larynx, 1996, Vol.23 (1), p.111-120 |
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| creator | Ohguro, Shinji Tsubota, Hiroshi |
| description | This study examined the expressions of a-subunits of very late antigen-6 (VLA-6; a6) and vitronectin receptor (VNR; αV) on tonsillar B cells and interactions between those integrins and their respective ligands, laminin (LM) and vitronectin (VN). α6 and αV were expressed on about 30 to 40% of tonsillar B cells. When purified tonsillar B cells were separated by a discontinuous Percoll gradient, the number of α6- and αV-positive cells decreased as the cell density went down, while the number of activated cells went up. After in vitro activation of tonsillar B cells by Staphylococcus aureus Cowan I strain (SAC), the expressions of a6 and aV and their adhesiveness to LM or VN decreased significantly. Increased proliferation of B cells was observed when tonsillar B cells were cultured with immobilized LM or VN. The results of immunohistological staining showed VLA-6, VNR, LM and VN in the follicular area. These results suggest that the expressions of VLA-6 and VNR on tonsillar B cells may be decreased during B cell activation, and the interaction between VLA-6, VNR, and LM, VN may give a costimulatory effect on B cell activation in the follicular area of the tonsi1. |
| doi_str_mv | 10.1016/S0385-8146(96)80017-4 |
| format | Article |
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When purified tonsillar B cells were separated by a discontinuous Percoll gradient, the number of α6- and αV-positive cells decreased as the cell density went down, while the number of activated cells went up. After in vitro activation of tonsillar B cells by Staphylococcus aureus Cowan I strain (SAC), the expressions of a6 and aV and their adhesiveness to LM or VN decreased significantly. Increased proliferation of B cells was observed when tonsillar B cells were cultured with immobilized LM or VN. The results of immunohistological staining showed VLA-6, VNR, LM and VN in the follicular area. These results suggest that the expressions of VLA-6 and VNR on tonsillar B cells may be decreased during B cell activation, and the interaction between VLA-6, VNR, and LM, VN may give a costimulatory effect on B cell activation in the follicular area of the tonsi1.</description><identifier>ISSN: 0385-8146</identifier><identifier>EISSN: 1879-1476</identifier><identifier>DOI: 10.1016/S0385-8146(96)80017-4</identifier><identifier>PMID: 8809332</identifier><language>eng</language><publisher>Netherlands: Elsevier Ireland Ltd</publisher><subject>Antibodies, Monoclonal ; B-Lymphocytes - physiology ; B7-1 Antigen - genetics ; Cell Adhesion ; Cell Movement ; Culture Techniques ; Flow Cytometry ; Humans ; Immunohistochemistry ; Laminin - physiology ; Ligands ; Palatine Tonsil - microbiology ; Random Allocation ; Receptors, Very Late Antigen - physiology ; Staphylococcus aureus ; Vitronectin - physiology</subject><ispartof>Auris, nasus, larynx, 1996, Vol.23 (1), p.111-120</ispartof><rights>1996 Society for Promotion of International Otorhinolaryngology (SPIO)</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c275t-29dffb08fff5df03c7fe359c3a764a09d08144bb051b04c2db2f749be0587afd3</citedby><cites>FETCH-LOGICAL-c275t-29dffb08fff5df03c7fe359c3a764a09d08144bb051b04c2db2f749be0587afd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0385-8146(96)80017-4$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,4024,27923,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8809332$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ohguro, Shinji</creatorcontrib><creatorcontrib>Tsubota, Hiroshi</creatorcontrib><title>Expressions of Very Late Antigen-6 and Vitronectin Receptor, and Their Interactions to Laminin and Vitronectin during Tonsillar B-Cell Activation</title><title>Auris, nasus, larynx</title><addtitle>Auris Nasus Larynx</addtitle><description>This study examined the expressions of a-subunits of very late antigen-6 (VLA-6; a6) and vitronectin receptor (VNR; αV) on tonsillar B cells and interactions between those integrins and their respective ligands, laminin (LM) and vitronectin (VN). α6 and αV were expressed on about 30 to 40% of tonsillar B cells. When purified tonsillar B cells were separated by a discontinuous Percoll gradient, the number of α6- and αV-positive cells decreased as the cell density went down, while the number of activated cells went up. After in vitro activation of tonsillar B cells by Staphylococcus aureus Cowan I strain (SAC), the expressions of a6 and aV and their adhesiveness to LM or VN decreased significantly. Increased proliferation of B cells was observed when tonsillar B cells were cultured with immobilized LM or VN. The results of immunohistological staining showed VLA-6, VNR, LM and VN in the follicular area. These results suggest that the expressions of VLA-6 and VNR on tonsillar B cells may be decreased during B cell activation, and the interaction between VLA-6, VNR, and LM, VN may give a costimulatory effect on B cell activation in the follicular area of the tonsi1.</description><subject>Antibodies, Monoclonal</subject><subject>B-Lymphocytes - physiology</subject><subject>B7-1 Antigen - genetics</subject><subject>Cell Adhesion</subject><subject>Cell Movement</subject><subject>Culture Techniques</subject><subject>Flow Cytometry</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Laminin - physiology</subject><subject>Ligands</subject><subject>Palatine Tonsil - microbiology</subject><subject>Random Allocation</subject><subject>Receptors, Very Late Antigen - physiology</subject><subject>Staphylococcus aureus</subject><subject>Vitronectin - physiology</subject><issn>0385-8146</issn><issn>1879-1476</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUctuEzEUtRBVSQufUMkrBFKntcczY3uFQlSgUqRKELq1PPZ1MZrYwXYq-hn8Mc5DXbBhdRfnce89B6ELSq4oocP1N8JE3wjaDe_k8F4QQnnTvUAzKrhsaMeHl2j2THmFznL-SQhhnMlTdCoEkYy1M_Tn5vcmQc4-hoyjw_eQnvBSF8DzUPwDhGbAOlh870uKAUzxAX8FA5sS0-UeWf0An_BtKJB0hXc-JVaLtQ-V-6_WbpMPD3hVaX6adMIfmwVME55X9FHv5K_RidNThjfHeY6-f7pZLb40y7vPt4v5sjEt70vTSuvcSIRzrreOMMMdsF4apvnQaSItqX9340h6OpLOtHZsHe_kCKQXXDvLztHbg-8mxV9byEWtfTb1Fh0gbrPigjFRA6zE_kA0KeacwKlN8mudnhQlaleF2lehdjkrOah9Faqruovjgu24BvusOmZf8Q8HHOqXjx6SysZDMGB9qmEpG_1_NvwFHPWbWw</recordid><startdate>1996</startdate><enddate>1996</enddate><creator>Ohguro, Shinji</creator><creator>Tsubota, Hiroshi</creator><general>Elsevier Ireland Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>8BM</scope></search><sort><creationdate>1996</creationdate><title>Expressions of Very Late Antigen-6 and Vitronectin Receptor, and Their Interactions to Laminin and Vitronectin during Tonsillar B-Cell Activation</title><author>Ohguro, Shinji ; Tsubota, Hiroshi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c275t-29dffb08fff5df03c7fe359c3a764a09d08144bb051b04c2db2f749be0587afd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Antibodies, Monoclonal</topic><topic>B-Lymphocytes - physiology</topic><topic>B7-1 Antigen - genetics</topic><topic>Cell Adhesion</topic><topic>Cell Movement</topic><topic>Culture Techniques</topic><topic>Flow Cytometry</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Laminin - physiology</topic><topic>Ligands</topic><topic>Palatine Tonsil - microbiology</topic><topic>Random Allocation</topic><topic>Receptors, Very Late Antigen - physiology</topic><topic>Staphylococcus aureus</topic><topic>Vitronectin - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ohguro, Shinji</creatorcontrib><creatorcontrib>Tsubota, Hiroshi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>ComDisDome</collection><jtitle>Auris, nasus, larynx</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ohguro, Shinji</au><au>Tsubota, Hiroshi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expressions of Very Late Antigen-6 and Vitronectin Receptor, and Their Interactions to Laminin and Vitronectin during Tonsillar B-Cell Activation</atitle><jtitle>Auris, nasus, larynx</jtitle><addtitle>Auris Nasus Larynx</addtitle><date>1996</date><risdate>1996</risdate><volume>23</volume><issue>1</issue><spage>111</spage><epage>120</epage><pages>111-120</pages><issn>0385-8146</issn><eissn>1879-1476</eissn><abstract>This study examined the expressions of a-subunits of very late antigen-6 (VLA-6; a6) and vitronectin receptor (VNR; αV) on tonsillar B cells and interactions between those integrins and their respective ligands, laminin (LM) and vitronectin (VN). α6 and αV were expressed on about 30 to 40% of tonsillar B cells. When purified tonsillar B cells were separated by a discontinuous Percoll gradient, the number of α6- and αV-positive cells decreased as the cell density went down, while the number of activated cells went up. After in vitro activation of tonsillar B cells by Staphylococcus aureus Cowan I strain (SAC), the expressions of a6 and aV and their adhesiveness to LM or VN decreased significantly. Increased proliferation of B cells was observed when tonsillar B cells were cultured with immobilized LM or VN. The results of immunohistological staining showed VLA-6, VNR, LM and VN in the follicular area. These results suggest that the expressions of VLA-6 and VNR on tonsillar B cells may be decreased during B cell activation, and the interaction between VLA-6, VNR, and LM, VN may give a costimulatory effect on B cell activation in the follicular area of the tonsi1.</abstract><cop>Netherlands</cop><pub>Elsevier Ireland Ltd</pub><pmid>8809332</pmid><doi>10.1016/S0385-8146(96)80017-4</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0385-8146 |
| ispartof | Auris, nasus, larynx, 1996, Vol.23 (1), p.111-120 |
| issn | 0385-8146 1879-1476 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Antibodies, Monoclonal B-Lymphocytes - physiology B7-1 Antigen - genetics Cell Adhesion Cell Movement Culture Techniques Flow Cytometry Humans Immunohistochemistry Laminin - physiology Ligands Palatine Tonsil - microbiology Random Allocation Receptors, Very Late Antigen - physiology Staphylococcus aureus Vitronectin - physiology |
| title | Expressions of Very Late Antigen-6 and Vitronectin Receptor, and Their Interactions to Laminin and Vitronectin during Tonsillar B-Cell Activation |
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