Integrated physical, genetic, and genic map covering 3 Mb around the human NGF gene (NGFB) at 1p13
We have characterized 11 overlapping yeast artificial chromosomes (YACs) in the 1p13 region, 8 of them containing the human nerve growth factor (NGF) gene (HGMW-approved symbol NGFB). Sequence-tagged sites (STSs) corresponding to YAC extremities have been designed and used for chromosome assignment...
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| Veröffentlicht in: | Genomics 1996, Vol.31 (1), p.80-89 |
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| container_title | Genomics |
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| creator | CARRIER, A ROSIER, M.-F GUILLEMOT, F GOGUEL, A.-F PULCINI, F BERNHEIM, A AUFFRAY, C DEVIGNES, M.-D |
| description | We have characterized 11 overlapping yeast artificial chromosomes (YACs) in the 1p13 region, 8 of them containing the human nerve growth factor (NGF) gene (HGMW-approved symbol NGFB). Sequence-tagged sites (STSs) corresponding to YAC extremities have been designed and used for chromosome assignment on a panel of monochromosomic somatic cell hybrids to check for YAC chimerism, in parallel with analyses by fluorescence in situ hybridization. Determination of end STS content and restriction mapping of the YACs led to the construction of a 3-Mb YAC contig. Four microsatellite markers from the Généthon collection and seven genes known to map to the 1p13 region have been ordered on the contig around the NGF gene. A new gene transcript from the Genexpress catalog has been localized on the contig. This work provides an integrated physical, genetic, and genic map of this chromosome 1 region. It constitutes a basis for determining the structure of the NGF gene and for further characterizing its genic environment. |
| doi_str_mv | 10.1006/geno.1996.0012 |
| format | Article |
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Sequence-tagged sites (STSs) corresponding to YAC extremities have been designed and used for chromosome assignment on a panel of monochromosomic somatic cell hybrids to check for YAC chimerism, in parallel with analyses by fluorescence in situ hybridization. Determination of end STS content and restriction mapping of the YACs led to the construction of a 3-Mb YAC contig. Four microsatellite markers from the Généthon collection and seven genes known to map to the 1p13 region have been ordered on the contig around the NGF gene. A new gene transcript from the Genexpress catalog has been localized on the contig. This work provides an integrated physical, genetic, and genic map of this chromosome 1 region. It constitutes a basis for determining the structure of the NGF gene and for further characterizing its genic environment.</description><identifier>ISSN: 0888-7543</identifier><identifier>EISSN: 1089-8646</identifier><identifier>DOI: 10.1006/geno.1996.0012</identifier><identifier>PMID: 8808283</identifier><language>eng</language><publisher>San Diego, CA: Elsevier</publisher><subject>Animals ; Base Sequence ; Biological and medical sciences ; BIOLOGICAL MARKERS ; BIOLOGY AND MEDICINE, BASIC STUDIES ; Chimera - genetics ; CHIMERAS ; Chromosome Mapping - methods ; Chromosomes, Artificial, Yeast ; Chromosomes, Human, Pair 1 - genetics ; Classical genetics, quantitative genetics, hybrids ; CONTIGS ; Cricetinae ; DESIGN ; DNA HYBRIDIZATION ; DNA Primers - genetics ; FLUORESCENCE ; Fundamental and applied biological sciences. Psychology ; GENES ; GENETIC MAPPING ; Genetic Markers ; Genetics of eukaryotes. Biological and molecular evolution ; GROWTH FACTORS ; Human ; HUMAN CHROMOSOME 1 ; Humans ; Hybrid Cells ; HYBRIDIZATION ; In Situ Hybridization, Fluorescence ; Mice ; Microsatellite Repeats ; Molecular Sequence Data ; Nerve Growth Factors - genetics ; NERVES ; POLYMERASE CHAIN REACTION ; Restriction Mapping ; Sequence Tagged Sites ; SOMATIC CELLS ; STRUCTURE-ACTIVITY RELATIONSHIPS ; TRANSCRIPTION</subject><ispartof>Genomics, 1996, Vol.31 (1), p.80-89</ispartof><rights>1996 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c277t-2193d2f74e2aed369f40dc0ccde750996c8e27ddf350af9e1b0337f7373ae6783</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,881,4010,27900,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2987975$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8808283$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/478837$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>CARRIER, A</creatorcontrib><creatorcontrib>ROSIER, M.-F</creatorcontrib><creatorcontrib>GUILLEMOT, F</creatorcontrib><creatorcontrib>GOGUEL, A.-F</creatorcontrib><creatorcontrib>PULCINI, F</creatorcontrib><creatorcontrib>BERNHEIM, A</creatorcontrib><creatorcontrib>AUFFRAY, C</creatorcontrib><creatorcontrib>DEVIGNES, M.-D</creatorcontrib><title>Integrated physical, genetic, and genic map covering 3 Mb around the human NGF gene (NGFB) at 1p13</title><title>Genomics</title><addtitle>Genomics</addtitle><description>We have characterized 11 overlapping yeast artificial chromosomes (YACs) in the 1p13 region, 8 of them containing the human nerve growth factor (NGF) gene (HGMW-approved symbol NGFB). Sequence-tagged sites (STSs) corresponding to YAC extremities have been designed and used for chromosome assignment on a panel of monochromosomic somatic cell hybrids to check for YAC chimerism, in parallel with analyses by fluorescence in situ hybridization. Determination of end STS content and restriction mapping of the YACs led to the construction of a 3-Mb YAC contig. Four microsatellite markers from the Généthon collection and seven genes known to map to the 1p13 region have been ordered on the contig around the NGF gene. A new gene transcript from the Genexpress catalog has been localized on the contig. This work provides an integrated physical, genetic, and genic map of this chromosome 1 region. It constitutes a basis for determining the structure of the NGF gene and for further characterizing its genic environment.</description><subject>Animals</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>BIOLOGICAL MARKERS</subject><subject>BIOLOGY AND MEDICINE, BASIC STUDIES</subject><subject>Chimera - genetics</subject><subject>CHIMERAS</subject><subject>Chromosome Mapping - methods</subject><subject>Chromosomes, Artificial, Yeast</subject><subject>Chromosomes, Human, Pair 1 - genetics</subject><subject>Classical genetics, quantitative genetics, hybrids</subject><subject>CONTIGS</subject><subject>Cricetinae</subject><subject>DESIGN</subject><subject>DNA HYBRIDIZATION</subject><subject>DNA Primers - genetics</subject><subject>FLUORESCENCE</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GENES</subject><subject>GENETIC MAPPING</subject><subject>Genetic Markers</subject><subject>Genetics of eukaryotes. Biological and molecular evolution</subject><subject>GROWTH FACTORS</subject><subject>Human</subject><subject>HUMAN CHROMOSOME 1</subject><subject>Humans</subject><subject>Hybrid Cells</subject><subject>HYBRIDIZATION</subject><subject>In Situ Hybridization, Fluorescence</subject><subject>Mice</subject><subject>Microsatellite Repeats</subject><subject>Molecular Sequence Data</subject><subject>Nerve Growth Factors - genetics</subject><subject>NERVES</subject><subject>POLYMERASE CHAIN REACTION</subject><subject>Restriction Mapping</subject><subject>Sequence Tagged Sites</subject><subject>SOMATIC CELLS</subject><subject>STRUCTURE-ACTIVITY RELATIONSHIPS</subject><subject>TRANSCRIPTION</subject><issn>0888-7543</issn><issn>1089-8646</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kEtr3DAURkVoSKeTbLMLqFBKC_FED9uSlm1oHpAmm3YtNNL1jMpYdiS5kH8fOTNkdXX5zv1AB6FzSlaUkPZqA2FYUaXaFSGUHaEFJVJVsq3bD2hBpJSVaGr-EX1K6R8hRHHJTtCJlEQyyRdofR8ybKLJ4PC4fUnemt0lLqWQvb3EJrh58Rb3ZsR2-A_Rhw3m-PcamzhMJc5bwNupNwE_3t68XeJv5fXzOzYZ05HyU3TcmV2Cs8Ncor83v_5c31UPT7f31z8eKsuEyBWjijvWiRqYAcdb1dXEWWKtA9GQ8kErgQnnOt4Q0ymga8K56AQX3EArJF-iz_veIWWvk_UZ7NYOIYDNuhZSFnKJvu6ZMQ7PE6Sse58s7HYmwDAlXWo4bxgr4GoP2jikFKHTY_S9iS-aEj2L17N4PYvXs_hycHFontY9uHf8YLrkXw65ScVxF02wPr1jTEmhRMNfAT4HiMo</recordid><startdate>1996</startdate><enddate>1996</enddate><creator>CARRIER, A</creator><creator>ROSIER, M.-F</creator><creator>GUILLEMOT, F</creator><creator>GOGUEL, A.-F</creator><creator>PULCINI, F</creator><creator>BERNHEIM, A</creator><creator>AUFFRAY, C</creator><creator>DEVIGNES, M.-D</creator><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>OTOTI</scope></search><sort><creationdate>1996</creationdate><title>Integrated physical, genetic, and genic map covering 3 Mb around the human NGF gene (NGFB) at 1p13</title><author>CARRIER, A ; ROSIER, M.-F ; GUILLEMOT, F ; GOGUEL, A.-F ; PULCINI, F ; BERNHEIM, A ; AUFFRAY, C ; DEVIGNES, M.-D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c277t-2193d2f74e2aed369f40dc0ccde750996c8e27ddf350af9e1b0337f7373ae6783</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Animals</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>BIOLOGICAL MARKERS</topic><topic>BIOLOGY AND MEDICINE, BASIC STUDIES</topic><topic>Chimera - genetics</topic><topic>CHIMERAS</topic><topic>Chromosome Mapping - methods</topic><topic>Chromosomes, Artificial, Yeast</topic><topic>Chromosomes, Human, Pair 1 - genetics</topic><topic>Classical genetics, quantitative genetics, hybrids</topic><topic>CONTIGS</topic><topic>Cricetinae</topic><topic>DESIGN</topic><topic>DNA HYBRIDIZATION</topic><topic>DNA Primers - genetics</topic><topic>FLUORESCENCE</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GENES</topic><topic>GENETIC MAPPING</topic><topic>Genetic Markers</topic><topic>Genetics of eukaryotes. Biological and molecular evolution</topic><topic>GROWTH FACTORS</topic><topic>Human</topic><topic>HUMAN CHROMOSOME 1</topic><topic>Humans</topic><topic>Hybrid Cells</topic><topic>HYBRIDIZATION</topic><topic>In Situ Hybridization, Fluorescence</topic><topic>Mice</topic><topic>Microsatellite Repeats</topic><topic>Molecular Sequence Data</topic><topic>Nerve Growth Factors - genetics</topic><topic>NERVES</topic><topic>POLYMERASE CHAIN REACTION</topic><topic>Restriction Mapping</topic><topic>Sequence Tagged Sites</topic><topic>SOMATIC CELLS</topic><topic>STRUCTURE-ACTIVITY RELATIONSHIPS</topic><topic>TRANSCRIPTION</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>CARRIER, A</creatorcontrib><creatorcontrib>ROSIER, M.-F</creatorcontrib><creatorcontrib>GUILLEMOT, F</creatorcontrib><creatorcontrib>GOGUEL, A.-F</creatorcontrib><creatorcontrib>PULCINI, F</creatorcontrib><creatorcontrib>BERNHEIM, A</creatorcontrib><creatorcontrib>AUFFRAY, C</creatorcontrib><creatorcontrib>DEVIGNES, M.-D</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV</collection><jtitle>Genomics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>CARRIER, A</au><au>ROSIER, M.-F</au><au>GUILLEMOT, F</au><au>GOGUEL, A.-F</au><au>PULCINI, F</au><au>BERNHEIM, A</au><au>AUFFRAY, C</au><au>DEVIGNES, M.-D</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Integrated physical, genetic, and genic map covering 3 Mb around the human NGF gene (NGFB) at 1p13</atitle><jtitle>Genomics</jtitle><addtitle>Genomics</addtitle><date>1996</date><risdate>1996</risdate><volume>31</volume><issue>1</issue><spage>80</spage><epage>89</epage><pages>80-89</pages><issn>0888-7543</issn><eissn>1089-8646</eissn><abstract>We have characterized 11 overlapping yeast artificial chromosomes (YACs) in the 1p13 region, 8 of them containing the human nerve growth factor (NGF) gene (HGMW-approved symbol NGFB). Sequence-tagged sites (STSs) corresponding to YAC extremities have been designed and used for chromosome assignment on a panel of monochromosomic somatic cell hybrids to check for YAC chimerism, in parallel with analyses by fluorescence in situ hybridization. Determination of end STS content and restriction mapping of the YACs led to the construction of a 3-Mb YAC contig. Four microsatellite markers from the Généthon collection and seven genes known to map to the 1p13 region have been ordered on the contig around the NGF gene. A new gene transcript from the Genexpress catalog has been localized on the contig. This work provides an integrated physical, genetic, and genic map of this chromosome 1 region. It constitutes a basis for determining the structure of the NGF gene and for further characterizing its genic environment.</abstract><cop>San Diego, CA</cop><pub>Elsevier</pub><pmid>8808283</pmid><doi>10.1006/geno.1996.0012</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0888-7543 |
| ispartof | Genomics, 1996, Vol.31 (1), p.80-89 |
| issn | 0888-7543 1089-8646 |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Base Sequence Biological and medical sciences BIOLOGICAL MARKERS BIOLOGY AND MEDICINE, BASIC STUDIES Chimera - genetics CHIMERAS Chromosome Mapping - methods Chromosomes, Artificial, Yeast Chromosomes, Human, Pair 1 - genetics Classical genetics, quantitative genetics, hybrids CONTIGS Cricetinae DESIGN DNA HYBRIDIZATION DNA Primers - genetics FLUORESCENCE Fundamental and applied biological sciences. Psychology GENES GENETIC MAPPING Genetic Markers Genetics of eukaryotes. Biological and molecular evolution GROWTH FACTORS Human HUMAN CHROMOSOME 1 Humans Hybrid Cells HYBRIDIZATION In Situ Hybridization, Fluorescence Mice Microsatellite Repeats Molecular Sequence Data Nerve Growth Factors - genetics NERVES POLYMERASE CHAIN REACTION Restriction Mapping Sequence Tagged Sites SOMATIC CELLS STRUCTURE-ACTIVITY RELATIONSHIPS TRANSCRIPTION |
| title | Integrated physical, genetic, and genic map covering 3 Mb around the human NGF gene (NGFB) at 1p13 |
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