Transdermal iontophoretic delivery of enkephalin formulated in liposomes
Transdermal iontophroetic transport of a liposomal formulation of [Leu5]enkephalin, across human cadaver skin, was investigated. Franz (vertical) cells were supplied with 0.5 mA/cm2 current density via silver/silver chloride electrodes from a Scepter power supply. Enkephalin spiked with [3H]enkephal...
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| Veröffentlicht in: | Journal of pharmaceutical sciences 1996-01, Vol.85 (1), p.5-8 |
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| creator | Vutla, Narendra B. Betageri, Guru V. Banga, Ajay K. |
| description | Transdermal iontophroetic transport of a liposomal formulation of [Leu5]enkephalin, across human cadaver skin, was investigated. Franz (vertical) cells were supplied with 0.5 mA/cm2 current density via silver/silver chloride electrodes from a Scepter power supply. Enkephalin spiked with [3H]enkephalin was transported across skin from anode or cathode, depending on the charge on the molecule. Liposomes or their constituents were shown to penetrate into the skin. Enkephalin, when delivered iontophoretically at its isoelectric point, from liposomes carrying positive or negative charge on their surface, resulted in permeation of radioactivity which was same or less than that of the controls when analyzed by liquid scintillation counting. When analyzed by radiochromatograpy detector on HPLC, degradation of enkephalin during transport was observed, with several degradation peaks in the chromatogram. The degradation was less in liposome formulations, as compared to controls. This is the first report of the combined use of liposomes and iontophoresis for transdermal delivery. |
| doi_str_mv | 10.1021/js950349y |
| format | Article |
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Franz (vertical) cells were supplied with 0.5 mA/cm2 current density via silver/silver chloride electrodes from a Scepter power supply. Enkephalin spiked with [3H]enkephalin was transported across skin from anode or cathode, depending on the charge on the molecule. Liposomes or their constituents were shown to penetrate into the skin. Enkephalin, when delivered iontophoretically at its isoelectric point, from liposomes carrying positive or negative charge on their surface, resulted in permeation of radioactivity which was same or less than that of the controls when analyzed by liquid scintillation counting. When analyzed by radiochromatograpy detector on HPLC, degradation of enkephalin during transport was observed, with several degradation peaks in the chromatogram. The degradation was less in liposome formulations, as compared to controls. This is the first report of the combined use of liposomes and iontophoresis for transdermal delivery.</description><identifier>ISSN: 0022-3549</identifier><identifier>EISSN: 1520-6017</identifier><identifier>DOI: 10.1021/js950349y</identifier><identifier>PMID: 8926583</identifier><identifier>CODEN: JPMSAE</identifier><language>eng</language><publisher>New York: John Wiley & Sons, Inc</publisher><subject>Administration, Cutaneous ; Biological and medical sciences ; Cadaver ; Chemistry, Pharmaceutical ; Enkephalin, Leucine - administration & dosage ; Enkephalin, Leucine - pharmacokinetics ; General pharmacology ; Humans ; Iontophoresis ; Isoelectric Point ; Liposomes ; Medical sciences ; Pharmacokinetics. Pharmacogenetics. Drug-receptor interactions ; Pharmacology. Drug treatments ; Skin Absorption</subject><ispartof>Journal of pharmaceutical sciences, 1996-01, Vol.85 (1), p.5-8</ispartof><rights>Copyright © 1996 Wiley‐Liss, Inc. and the American Pharmaceutical Association</rights><rights>1996 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1021%2Fjs950349y$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1021%2Fjs950349y$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,4010,27900,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3009292$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8926583$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Vutla, Narendra B.</creatorcontrib><creatorcontrib>Betageri, Guru V.</creatorcontrib><creatorcontrib>Banga, Ajay K.</creatorcontrib><title>Transdermal iontophoretic delivery of enkephalin formulated in liposomes</title><title>Journal of pharmaceutical sciences</title><addtitle>J. Pharm. Sci</addtitle><description>Transdermal iontophroetic transport of a liposomal formulation of [Leu5]enkephalin, across human cadaver skin, was investigated. Franz (vertical) cells were supplied with 0.5 mA/cm2 current density via silver/silver chloride electrodes from a Scepter power supply. Enkephalin spiked with [3H]enkephalin was transported across skin from anode or cathode, depending on the charge on the molecule. Liposomes or their constituents were shown to penetrate into the skin. Enkephalin, when delivered iontophoretically at its isoelectric point, from liposomes carrying positive or negative charge on their surface, resulted in permeation of radioactivity which was same or less than that of the controls when analyzed by liquid scintillation counting. When analyzed by radiochromatograpy detector on HPLC, degradation of enkephalin during transport was observed, with several degradation peaks in the chromatogram. The degradation was less in liposome formulations, as compared to controls. This is the first report of the combined use of liposomes and iontophoresis for transdermal delivery.</description><subject>Administration, Cutaneous</subject><subject>Biological and medical sciences</subject><subject>Cadaver</subject><subject>Chemistry, Pharmaceutical</subject><subject>Enkephalin, Leucine - administration & dosage</subject><subject>Enkephalin, Leucine - pharmacokinetics</subject><subject>General pharmacology</subject><subject>Humans</subject><subject>Iontophoresis</subject><subject>Isoelectric Point</subject><subject>Liposomes</subject><subject>Medical sciences</subject><subject>Pharmacokinetics. Pharmacogenetics. Drug-receptor interactions</subject><subject>Pharmacology. Drug treatments</subject><subject>Skin Absorption</subject><issn>0022-3549</issn><issn>1520-6017</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo90F1r2zAUBmBROtIs60V_QMEXZTfDqz4sybpsw5ZshHahKYPdCEU-Jkply5Wcdfn3c0nI1UG8D-KcF6Ergr8STMntNimOWaH2Z2hMOMW5wESeozHGlOaMF-oCfUxpizEWmPMRGpWKCl6yMZqvomlTBbExPnOh7UO3CRF6Z7MKvPsLcZ-FOoP2BbqN8a7N6hCbnTc9VNnw8q4LKTSQPqEPtfEJLo9zgp6_f1tN5_nicfZjerfIHcOK5rTgcl1JKkgtjSWEMskpcFLWBvPCllLUyhoFFSe2KES1rhinsrSiYMwIrNgEfT7828XwuoPU68YlC96bFsIuaVkyqogSA7w-wt26gUp30TUm7vXx9CG_OeYmWeProQfr0okxPKyr6MC-HNib87A_xQTr9-b1qXn989fTu84P2qUe_p20iS9ayOFU_fthpudLtliuln_0PfsPcYeD8Q</recordid><startdate>199601</startdate><enddate>199601</enddate><creator>Vutla, Narendra B.</creator><creator>Betageri, Guru V.</creator><creator>Banga, Ajay K.</creator><general>John Wiley & Sons, Inc</general><general>Wiley</general><general>American Pharmaceutical Association</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>199601</creationdate><title>Transdermal iontophoretic delivery of enkephalin formulated in liposomes</title><author>Vutla, Narendra B. ; Betageri, Guru V. ; Banga, Ajay K.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-i3092-2457bd7261f7ac1123752e518fa054c876f9ca9ed51c446dbd35278c6433a6093</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Administration, Cutaneous</topic><topic>Biological and medical sciences</topic><topic>Cadaver</topic><topic>Chemistry, Pharmaceutical</topic><topic>Enkephalin, Leucine - administration & dosage</topic><topic>Enkephalin, Leucine - pharmacokinetics</topic><topic>General pharmacology</topic><topic>Humans</topic><topic>Iontophoresis</topic><topic>Isoelectric Point</topic><topic>Liposomes</topic><topic>Medical sciences</topic><topic>Pharmacokinetics. Pharmacogenetics. Drug-receptor interactions</topic><topic>Pharmacology. Drug treatments</topic><topic>Skin Absorption</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Vutla, Narendra B.</creatorcontrib><creatorcontrib>Betageri, Guru V.</creatorcontrib><creatorcontrib>Banga, Ajay K.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of pharmaceutical sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Vutla, Narendra B.</au><au>Betageri, Guru V.</au><au>Banga, Ajay K.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Transdermal iontophoretic delivery of enkephalin formulated in liposomes</atitle><jtitle>Journal of pharmaceutical sciences</jtitle><addtitle>J. Pharm. Sci</addtitle><date>1996-01</date><risdate>1996</risdate><volume>85</volume><issue>1</issue><spage>5</spage><epage>8</epage><pages>5-8</pages><issn>0022-3549</issn><eissn>1520-6017</eissn><coden>JPMSAE</coden><abstract>Transdermal iontophroetic transport of a liposomal formulation of [Leu5]enkephalin, across human cadaver skin, was investigated. Franz (vertical) cells were supplied with 0.5 mA/cm2 current density via silver/silver chloride electrodes from a Scepter power supply. Enkephalin spiked with [3H]enkephalin was transported across skin from anode or cathode, depending on the charge on the molecule. Liposomes or their constituents were shown to penetrate into the skin. Enkephalin, when delivered iontophoretically at its isoelectric point, from liposomes carrying positive or negative charge on their surface, resulted in permeation of radioactivity which was same or less than that of the controls when analyzed by liquid scintillation counting. When analyzed by radiochromatograpy detector on HPLC, degradation of enkephalin during transport was observed, with several degradation peaks in the chromatogram. The degradation was less in liposome formulations, as compared to controls. This is the first report of the combined use of liposomes and iontophoresis for transdermal delivery.</abstract><cop>New York</cop><pub>John Wiley & Sons, Inc</pub><pmid>8926583</pmid><doi>10.1021/js950349y</doi><tpages>4</tpages></addata></record> |
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| ispartof | Journal of pharmaceutical sciences, 1996-01, Vol.85 (1), p.5-8 |
| issn | 0022-3549 1520-6017 |
| language | eng |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; Alma/SFX Local Collection |
| subjects | Administration, Cutaneous Biological and medical sciences Cadaver Chemistry, Pharmaceutical Enkephalin, Leucine - administration & dosage Enkephalin, Leucine - pharmacokinetics General pharmacology Humans Iontophoresis Isoelectric Point Liposomes Medical sciences Pharmacokinetics. Pharmacogenetics. Drug-receptor interactions Pharmacology. Drug treatments Skin Absorption |
| title | Transdermal iontophoretic delivery of enkephalin formulated in liposomes |
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