Specific Metal-Oligonucleotide Binding Studied by High Resolution Tandem Mass Spectrometry
Electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry (ESI‐FTICR‐MS) was used to study the binding of metal ions to two oligonucleotides, d(pGCTTGCATp) and d(TTGGCCCTCCTT). Collision‐induced dissociation (CID) of the metal–oligonucleotide complex revealed that metal ion...
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| Veröffentlicht in: | Journal of mass spectrometry. 1996-06, Vol.31 (6), p.669-675 |
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| container_title | Journal of mass spectrometry. |
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| creator | Wu, Qinyuan Cheng, Xueheng Hofstadler, Steven A. Smith, Richard D. |
| description | Electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry (ESI‐FTICR‐MS) was used to study the binding of metal ions to two oligonucleotides, d(pGCTTGCATp) and d(TTGGCCCTCCTT). Collision‐induced dissociation (CID) of the metal–oligonucleotide complex revealed that metal ions preferentially bound to the central thymine region of the sequence. The most probable binding sites were the phosphodiester backbone since the sum of the maximum number of charge addition from the metal ions and the charge state of the whole complex was found to be equal to the number of ionizable protons on the DNA backbone. Although site‐specific and sequence‐specific binding was observed for all three of the metal ions studied, the binding specificity of UO22+ ions was significantly greater than for Mg2+ and Na+. These experiments demonstrate that ESI‐MS/MS can be applied to study the binding of metal ions and their complexes to oligonucleotides, providing not only information on the number of metal ions binding to the oligonucleotide, but also information related to the binding site(s) and binding specificity. |
| doi_str_mv | 10.1002/(SICI)1096-9888(199606)31:6<669::AID-JMS340>3.0.CO;2-5 |
| format | Article |
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Collision‐induced dissociation (CID) of the metal–oligonucleotide complex revealed that metal ions preferentially bound to the central thymine region of the sequence. The most probable binding sites were the phosphodiester backbone since the sum of the maximum number of charge addition from the metal ions and the charge state of the whole complex was found to be equal to the number of ionizable protons on the DNA backbone. Although site‐specific and sequence‐specific binding was observed for all three of the metal ions studied, the binding specificity of UO22+ ions was significantly greater than for Mg2+ and Na+. These experiments demonstrate that ESI‐MS/MS can be applied to study the binding of metal ions and their complexes to oligonucleotides, providing not only information on the number of metal ions binding to the oligonucleotide, but also information related to the binding site(s) and binding specificity.</description><identifier>ISSN: 1076-5174</identifier><identifier>EISSN: 1096-9888</identifier><identifier>DOI: 10.1002/(SICI)1096-9888(199606)31:6<669::AID-JMS340>3.0.CO;2-5</identifier><identifier>PMID: 8799301</identifier><language>eng</language><publisher>Sussex: John Wiley & Sons, Ltd</publisher><subject>Analytical, structural and metabolic biochemistry ; Biological and medical sciences ; collision-induced dissociation ; Cyclotrons ; DNA Adducts - analysis ; Dna, deoxyribonucleoproteins ; electrospray ; Flame Ionization ; Fourier Analysis ; Fourier transform ion cyclotron resonance ; Fundamental and applied biological sciences. Psychology ; Gas Chromatography-Mass Spectrometry ; metal binding ; Metals - chemistry ; Nucleic acids ; oligonucleotides ; Oligonucleotides - chemistry ; Thymidine - chemistry</subject><ispartof>Journal of mass spectrometry., 1996-06, Vol.31 (6), p.669-675</ispartof><rights>Copyright © 1996 John Wiley & Sons, Ltd.</rights><rights>1996 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2F%28SICI%291096-9888%28199606%2931%3A6%3C669%3A%3AAID-JMS340%3E3.0.CO%3B2-5$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2F%28SICI%291096-9888%28199606%2931%3A6%3C669%3A%3AAID-JMS340%3E3.0.CO%3B2-5$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3107971$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8799301$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wu, Qinyuan</creatorcontrib><creatorcontrib>Cheng, Xueheng</creatorcontrib><creatorcontrib>Hofstadler, Steven A.</creatorcontrib><creatorcontrib>Smith, Richard D.</creatorcontrib><title>Specific Metal-Oligonucleotide Binding Studied by High Resolution Tandem Mass Spectrometry</title><title>Journal of mass spectrometry.</title><addtitle>J. Mass Spectrom</addtitle><description>Electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry (ESI‐FTICR‐MS) was used to study the binding of metal ions to two oligonucleotides, d(pGCTTGCATp) and d(TTGGCCCTCCTT). Collision‐induced dissociation (CID) of the metal–oligonucleotide complex revealed that metal ions preferentially bound to the central thymine region of the sequence. The most probable binding sites were the phosphodiester backbone since the sum of the maximum number of charge addition from the metal ions and the charge state of the whole complex was found to be equal to the number of ionizable protons on the DNA backbone. Although site‐specific and sequence‐specific binding was observed for all three of the metal ions studied, the binding specificity of UO22+ ions was significantly greater than for Mg2+ and Na+. These experiments demonstrate that ESI‐MS/MS can be applied to study the binding of metal ions and their complexes to oligonucleotides, providing not only information on the number of metal ions binding to the oligonucleotide, but also information related to the binding site(s) and binding specificity.</description><subject>Analytical, structural and metabolic biochemistry</subject><subject>Biological and medical sciences</subject><subject>collision-induced dissociation</subject><subject>Cyclotrons</subject><subject>DNA Adducts - analysis</subject><subject>Dna, deoxyribonucleoproteins</subject><subject>electrospray</subject><subject>Flame Ionization</subject><subject>Fourier Analysis</subject><subject>Fourier transform ion cyclotron resonance</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gas Chromatography-Mass Spectrometry</subject><subject>metal binding</subject><subject>Metals - chemistry</subject><subject>Nucleic acids</subject><subject>oligonucleotides</subject><subject>Oligonucleotides - chemistry</subject><subject>Thymidine - chemistry</subject><issn>1076-5174</issn><issn>1096-9888</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkV1v0zAUhiMEGmPwE5BygdB2keKPxI4LmrQFthXWVayDIW6OXPukGPJR4kTQf0-iVL0BiStbPq8fvXpOEJxSMqGEsFfHy1k2O6FEiUilaXpMlRJEnHA6FW-EUNPp2ext9H6-5DE55RMyyRavWZQ8CA73Xx4OdymihMr4cfDE---EEKVicRAcpFIpTuhh8HW5QeNyZ8I5trqIFoVb11VnCqxbZzE8d5V11Tpctp11aMPVNrxy62_hLfq66FpXV-GdriyW4Vx7Hw60tqlLbJvt0-BRrguPz3bnUfDp4t1ddhVdLy5n2dl1ZGLOSSQsJzJnaFhihWQ0zZXVK8lSVCaWNNbcJoZpmsrYUB7nmpGVVmiSOGcJTTU_Cl6O3E1T_-zQt1A6b7AodIV150GmnAkhZR_8PAZNU3vfYA6bxpW62QIlMEgHGKTDYBAGgzBKB05BQC8doJcOo3TgQCBbAIOkBz_fNehWJdo9dme5n7_YzbU3usgbXRnn9zHer0nJIfZljP1yBW7_Kvefbv-stnvp0dGIdr7F33u0bn6AkFwmcH9zCecfLj5mt-webvgfJxK5Wg</recordid><startdate>199606</startdate><enddate>199606</enddate><creator>Wu, Qinyuan</creator><creator>Cheng, Xueheng</creator><creator>Hofstadler, Steven A.</creator><creator>Smith, Richard D.</creator><general>John Wiley & Sons, Ltd</general><general>Wiley</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199606</creationdate><title>Specific Metal-Oligonucleotide Binding Studied by High Resolution Tandem Mass Spectrometry</title><author>Wu, Qinyuan ; Cheng, Xueheng ; Hofstadler, Steven A. ; Smith, Richard D.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4330-6d307f2ec25d67218f9dab728e9c4714a3d5c2a1874c134fa20ba9ec54f2518a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Analytical, structural and metabolic biochemistry</topic><topic>Biological and medical sciences</topic><topic>collision-induced dissociation</topic><topic>Cyclotrons</topic><topic>DNA Adducts - analysis</topic><topic>Dna, deoxyribonucleoproteins</topic><topic>electrospray</topic><topic>Flame Ionization</topic><topic>Fourier Analysis</topic><topic>Fourier transform ion cyclotron resonance</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gas Chromatography-Mass Spectrometry</topic><topic>metal binding</topic><topic>Metals - chemistry</topic><topic>Nucleic acids</topic><topic>oligonucleotides</topic><topic>Oligonucleotides - chemistry</topic><topic>Thymidine - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wu, Qinyuan</creatorcontrib><creatorcontrib>Cheng, Xueheng</creatorcontrib><creatorcontrib>Hofstadler, Steven A.</creatorcontrib><creatorcontrib>Smith, Richard D.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of mass spectrometry.</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wu, Qinyuan</au><au>Cheng, Xueheng</au><au>Hofstadler, Steven A.</au><au>Smith, Richard D.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Specific Metal-Oligonucleotide Binding Studied by High Resolution Tandem Mass Spectrometry</atitle><jtitle>Journal of mass spectrometry.</jtitle><addtitle>J. Mass Spectrom</addtitle><date>1996-06</date><risdate>1996</risdate><volume>31</volume><issue>6</issue><spage>669</spage><epage>675</epage><pages>669-675</pages><issn>1076-5174</issn><eissn>1096-9888</eissn><abstract>Electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry (ESI‐FTICR‐MS) was used to study the binding of metal ions to two oligonucleotides, d(pGCTTGCATp) and d(TTGGCCCTCCTT). Collision‐induced dissociation (CID) of the metal–oligonucleotide complex revealed that metal ions preferentially bound to the central thymine region of the sequence. The most probable binding sites were the phosphodiester backbone since the sum of the maximum number of charge addition from the metal ions and the charge state of the whole complex was found to be equal to the number of ionizable protons on the DNA backbone. Although site‐specific and sequence‐specific binding was observed for all three of the metal ions studied, the binding specificity of UO22+ ions was significantly greater than for Mg2+ and Na+. These experiments demonstrate that ESI‐MS/MS can be applied to study the binding of metal ions and their complexes to oligonucleotides, providing not only information on the number of metal ions binding to the oligonucleotide, but also information related to the binding site(s) and binding specificity.</abstract><cop>Sussex</cop><pub>John Wiley & Sons, Ltd</pub><pmid>8799301</pmid><doi>10.1002/(SICI)1096-9888(199606)31:6<669::AID-JMS340>3.0.CO;2-5</doi><tpages>7</tpages></addata></record> |
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| ispartof | Journal of mass spectrometry., 1996-06, Vol.31 (6), p.669-675 |
| issn | 1076-5174 1096-9888 |
| language | eng |
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| subjects | Analytical, structural and metabolic biochemistry Biological and medical sciences collision-induced dissociation Cyclotrons DNA Adducts - analysis Dna, deoxyribonucleoproteins electrospray Flame Ionization Fourier Analysis Fourier transform ion cyclotron resonance Fundamental and applied biological sciences. Psychology Gas Chromatography-Mass Spectrometry metal binding Metals - chemistry Nucleic acids oligonucleotides Oligonucleotides - chemistry Thymidine - chemistry |
| title | Specific Metal-Oligonucleotide Binding Studied by High Resolution Tandem Mass Spectrometry |
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