Expression of dopamine transporter mRNA and its binding site in fetal nigral cells transplanted into the striatum of 6-OHDA lesioned rat
Neurological disorders in rat model of hemi-Parkinson's disease can be compensated by the transplantation of fetal nigral cells. However, the role of the dopamine transporter (DAT) in this recovery has not been clarified. To clarify this mechanism, we examined the expression of DAT in the cauda...
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| Veröffentlicht in: | Brain research. Molecular brain research. 1996-07, Vol.39 (1), p.127-136 |
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| creator | Fujita, Masahiro Nishino, Hitoo Kumazaki, Michiko Shimada, Shoichi Tohyama, Masaya Nishimura, Tsunehiko |
| description | Neurological disorders in rat model of hemi-Parkinson's disease can be compensated by the transplantation of fetal nigral cells. However, the role of the dopamine transporter (DAT) in this recovery has not been clarified. To clarify this mechanism, we examined the expression of DAT in the caudate putamen (CPu) by in situ hybridization histochemistry (mRNA) and autoradiography (using the ligand [
125I]β-CIT, which labels DAT) and compared them with the recovery of motor disturbance revealed with methamphetamine-induced rotation. Models were made with the stereotaxic injection of 6-hydroxydopamine into the side of the substantia nigra pars compacta. Cell suspensions from rat fetus (embryonic day 14–15) were transplanted into the lesioned side of CPu. Methamphetamine-induced rotation, expression of DAT mRNA, and [
125I]β-CIT binding evaluated 2,4 and 12 week after the transplantation. Metamphetamine-induced rotation recovered partly in the 2nd week and significantly in the 4th week. [
125I]β-CIT binding increased with time and the dense binding was detected 4 and 12 weeks after the plantation. In all transplanted rats, cell expressing DAT mRNA were found in CPu. These results indicated that transplanted fetal dopaminergic cells in maturared in CPu of host animals and extended nerve terminals where high density of DAT binding sites were found. |
| doi_str_mv | 10.1016/0169-328X(96)00018-6 |
| format | Article |
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125I]β-CIT, which labels DAT) and compared them with the recovery of motor disturbance revealed with methamphetamine-induced rotation. Models were made with the stereotaxic injection of 6-hydroxydopamine into the side of the substantia nigra pars compacta. Cell suspensions from rat fetus (embryonic day 14–15) were transplanted into the lesioned side of CPu. Methamphetamine-induced rotation, expression of DAT mRNA, and [
125I]β-CIT binding evaluated 2,4 and 12 week after the transplantation. Metamphetamine-induced rotation recovered partly in the 2nd week and significantly in the 4th week. [
125I]β-CIT binding increased with time and the dense binding was detected 4 and 12 weeks after the plantation. In all transplanted rats, cell expressing DAT mRNA were found in CPu. These results indicated that transplanted fetal dopaminergic cells in maturared in CPu of host animals and extended nerve terminals where high density of DAT binding sites were found.</description><identifier>ISSN: 0169-328X</identifier><identifier>EISSN: 1872-6941</identifier><identifier>DOI: 10.1016/0169-328X(96)00018-6</identifier><identifier>PMID: 8804721</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Animals ; Autoradiography ; Behavior, Animal - physiology ; Binding, Competitive ; Biological and medical sciences ; Brain Tissue Transplantation ; Carrier Proteins - metabolism ; Caudate putamen ; Cell Transplantation ; Corpus Striatum - metabolism ; Development. Senescence. Regeneration. Transplantation ; Dopamine Plasma Membrane Transport Proteins ; Dopamine transporter ; Female ; Fundamental and applied biological sciences. Psychology ; In Situ Hybridization ; Membrane Glycoproteins ; Membrane Transport Proteins ; Nerve Tissue Proteins ; Oxidopamine - pharmacology ; Parkinson's disease ; Rats ; Rats, Wistar ; Substantia nigra ; Substantia Nigra - metabolism ; Transplantation ; Vertebrates: nervous system and sense organs</subject><ispartof>Brain research. Molecular brain research., 1996-07, Vol.39 (1), p.127-136</ispartof><rights>1996</rights><rights>1996 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c432t-d6dd264897108067686e98b6c19a6322544851eb853d15fb4a1eda54b07643433</citedby><cites>FETCH-LOGICAL-c432t-d6dd264897108067686e98b6c19a6322544851eb853d15fb4a1eda54b07643433</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3145368$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8804721$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fujita, Masahiro</creatorcontrib><creatorcontrib>Nishino, Hitoo</creatorcontrib><creatorcontrib>Kumazaki, Michiko</creatorcontrib><creatorcontrib>Shimada, Shoichi</creatorcontrib><creatorcontrib>Tohyama, Masaya</creatorcontrib><creatorcontrib>Nishimura, Tsunehiko</creatorcontrib><title>Expression of dopamine transporter mRNA and its binding site in fetal nigral cells transplanted into the striatum of 6-OHDA lesioned rat</title><title>Brain research. Molecular brain research.</title><addtitle>Brain Res Mol Brain Res</addtitle><description>Neurological disorders in rat model of hemi-Parkinson's disease can be compensated by the transplantation of fetal nigral cells. However, the role of the dopamine transporter (DAT) in this recovery has not been clarified. To clarify this mechanism, we examined the expression of DAT in the caudate putamen (CPu) by in situ hybridization histochemistry (mRNA) and autoradiography (using the ligand [
125I]β-CIT, which labels DAT) and compared them with the recovery of motor disturbance revealed with methamphetamine-induced rotation. Models were made with the stereotaxic injection of 6-hydroxydopamine into the side of the substantia nigra pars compacta. Cell suspensions from rat fetus (embryonic day 14–15) were transplanted into the lesioned side of CPu. Methamphetamine-induced rotation, expression of DAT mRNA, and [
125I]β-CIT binding evaluated 2,4 and 12 week after the transplantation. Metamphetamine-induced rotation recovered partly in the 2nd week and significantly in the 4th week. [
125I]β-CIT binding increased with time and the dense binding was detected 4 and 12 weeks after the plantation. In all transplanted rats, cell expressing DAT mRNA were found in CPu. These results indicated that transplanted fetal dopaminergic cells in maturared in CPu of host animals and extended nerve terminals where high density of DAT binding sites were found.</description><subject>Animals</subject><subject>Autoradiography</subject><subject>Behavior, Animal - physiology</subject><subject>Binding, Competitive</subject><subject>Biological and medical sciences</subject><subject>Brain Tissue Transplantation</subject><subject>Carrier Proteins - metabolism</subject><subject>Caudate putamen</subject><subject>Cell Transplantation</subject><subject>Corpus Striatum - metabolism</subject><subject>Development. Senescence. Regeneration. Transplantation</subject><subject>Dopamine Plasma Membrane Transport Proteins</subject><subject>Dopamine transporter</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>In Situ Hybridization</subject><subject>Membrane Glycoproteins</subject><subject>Membrane Transport Proteins</subject><subject>Nerve Tissue Proteins</subject><subject>Oxidopamine - pharmacology</subject><subject>Parkinson's disease</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Substantia nigra</subject><subject>Substantia Nigra - metabolism</subject><subject>Transplantation</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0169-328X</issn><issn>1872-6941</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kdGK1TAQhoMo63H1DRRyIaIX1aRJ0_RGOKyrKywuiIJ3IU2ma6RNu5kc0TfwsU095Vx6EYYw358ZvhDylLPXnHH1ppyuErX-9rJTrxhjXFfqHtlx3daV6iS_T3Yn5CF5hPjjH8T5GTnTmsm25jvy5_LXkgAxzJHOA_XzYqcQgeZkIy5zypDo9PnTntroachI-xB9iLcUQwYaIh0g25HGcJtKcTCOuGVHGzOUTMwzzd-BYk7B5sO0jlHVzdW7PR1hnVugZPNj8mCwI8KTrZ6Tr-8vv1xcVdc3Hz5e7K8rJ0WdK6-8r5XUXcuZZqpVWkGne-V4Z5Wo60ZK3XDodSM8b4ZeWg7eNrJnrZJCCnFOXhzfXdJ8dwDMZgq47m0jzAc0rRa8FU1dQHkEXZoREwxmSWGy6bfhzKwfYFa7ZrVrunJZ3RpVYs-29w_9BP4U2oyX_vOtb9HZcSiuXMATJrhshNIFe3vEoLj4GSAZdAGiAx8SuGz8HP6_x1-90qHz</recordid><startdate>19960701</startdate><enddate>19960701</enddate><creator>Fujita, Masahiro</creator><creator>Nishino, Hitoo</creator><creator>Kumazaki, Michiko</creator><creator>Shimada, Shoichi</creator><creator>Tohyama, Masaya</creator><creator>Nishimura, Tsunehiko</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19960701</creationdate><title>Expression of dopamine transporter mRNA and its binding site in fetal nigral cells transplanted into the striatum of 6-OHDA lesioned rat</title><author>Fujita, Masahiro ; Nishino, Hitoo ; Kumazaki, Michiko ; Shimada, Shoichi ; Tohyama, Masaya ; Nishimura, Tsunehiko</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c432t-d6dd264897108067686e98b6c19a6322544851eb853d15fb4a1eda54b07643433</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Animals</topic><topic>Autoradiography</topic><topic>Behavior, Animal - physiology</topic><topic>Binding, Competitive</topic><topic>Biological and medical sciences</topic><topic>Brain Tissue Transplantation</topic><topic>Carrier Proteins - metabolism</topic><topic>Caudate putamen</topic><topic>Cell Transplantation</topic><topic>Corpus Striatum - metabolism</topic><topic>Development. Senescence. Regeneration. Transplantation</topic><topic>Dopamine Plasma Membrane Transport Proteins</topic><topic>Dopamine transporter</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>In Situ Hybridization</topic><topic>Membrane Glycoproteins</topic><topic>Membrane Transport Proteins</topic><topic>Nerve Tissue Proteins</topic><topic>Oxidopamine - pharmacology</topic><topic>Parkinson's disease</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Substantia nigra</topic><topic>Substantia Nigra - metabolism</topic><topic>Transplantation</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fujita, Masahiro</creatorcontrib><creatorcontrib>Nishino, Hitoo</creatorcontrib><creatorcontrib>Kumazaki, Michiko</creatorcontrib><creatorcontrib>Shimada, Shoichi</creatorcontrib><creatorcontrib>Tohyama, Masaya</creatorcontrib><creatorcontrib>Nishimura, Tsunehiko</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Brain research. Molecular brain research.</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fujita, Masahiro</au><au>Nishino, Hitoo</au><au>Kumazaki, Michiko</au><au>Shimada, Shoichi</au><au>Tohyama, Masaya</au><au>Nishimura, Tsunehiko</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of dopamine transporter mRNA and its binding site in fetal nigral cells transplanted into the striatum of 6-OHDA lesioned rat</atitle><jtitle>Brain research. Molecular brain research.</jtitle><addtitle>Brain Res Mol Brain Res</addtitle><date>1996-07-01</date><risdate>1996</risdate><volume>39</volume><issue>1</issue><spage>127</spage><epage>136</epage><pages>127-136</pages><issn>0169-328X</issn><eissn>1872-6941</eissn><abstract>Neurological disorders in rat model of hemi-Parkinson's disease can be compensated by the transplantation of fetal nigral cells. However, the role of the dopamine transporter (DAT) in this recovery has not been clarified. To clarify this mechanism, we examined the expression of DAT in the caudate putamen (CPu) by in situ hybridization histochemistry (mRNA) and autoradiography (using the ligand [
125I]β-CIT, which labels DAT) and compared them with the recovery of motor disturbance revealed with methamphetamine-induced rotation. Models were made with the stereotaxic injection of 6-hydroxydopamine into the side of the substantia nigra pars compacta. Cell suspensions from rat fetus (embryonic day 14–15) were transplanted into the lesioned side of CPu. Methamphetamine-induced rotation, expression of DAT mRNA, and [
125I]β-CIT binding evaluated 2,4 and 12 week after the transplantation. Metamphetamine-induced rotation recovered partly in the 2nd week and significantly in the 4th week. [
125I]β-CIT binding increased with time and the dense binding was detected 4 and 12 weeks after the plantation. In all transplanted rats, cell expressing DAT mRNA were found in CPu. These results indicated that transplanted fetal dopaminergic cells in maturared in CPu of host animals and extended nerve terminals where high density of DAT binding sites were found.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>8804721</pmid><doi>10.1016/0169-328X(96)00018-6</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Autoradiography Behavior, Animal - physiology Binding, Competitive Biological and medical sciences Brain Tissue Transplantation Carrier Proteins - metabolism Caudate putamen Cell Transplantation Corpus Striatum - metabolism Development. Senescence. Regeneration. Transplantation Dopamine Plasma Membrane Transport Proteins Dopamine transporter Female Fundamental and applied biological sciences. Psychology In Situ Hybridization Membrane Glycoproteins Membrane Transport Proteins Nerve Tissue Proteins Oxidopamine - pharmacology Parkinson's disease Rats Rats, Wistar Substantia nigra Substantia Nigra - metabolism Transplantation Vertebrates: nervous system and sense organs |
| title | Expression of dopamine transporter mRNA and its binding site in fetal nigral cells transplanted into the striatum of 6-OHDA lesioned rat |
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