Rapid increase in inducible nitric oxide synthase gene expression in the heart during endotoxemia
Inducible, Ca 2+-independent nitric oxide (NO) synthase activity in the heart is elevated during endotoxemia and the resulting excess release of NO depresses cardiac contractile function. We show here that this is due to an extremely rapid induction of inducible NO synthase gene expression. Followin...
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| Veröffentlicht in: | European journal of pharmacology 1996-05, Vol.303 (1), p.141-144 |
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| container_title | European journal of pharmacology |
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| creator | Bateson, Alan N. Jakiwczyk, Olga M. Schulz, Richard |
| description | Inducible, Ca
2+-independent nitric oxide (NO) synthase activity in the heart is elevated during endotoxemia and the resulting excess release of NO depresses cardiac contractile function. We show here that this is due to an extremely rapid induction of inducible NO synthase gene expression. Following injection of endotoxin (bacterial lipopolysaccharide) in rats we detected increased inducible NO synthase mRNA levels in the left ventricular wall within 30 min which then peaked at 3 h. This was followed by an increase in myocardial inducible NO synthase enzyme activity and plasma levels of NO metabolites, nitrate and nitrite, which peaked at 6 and 12 h, respectively. The extremely rapid induction of inducible NO synthase may serve to protect the heart against microbial infection and concomitantly alter myocardial mechanical function. |
| doi_str_mv | 10.1016/0014-2999(96)00099-4 |
| format | Article |
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2+-independent nitric oxide (NO) synthase activity in the heart is elevated during endotoxemia and the resulting excess release of NO depresses cardiac contractile function. We show here that this is due to an extremely rapid induction of inducible NO synthase gene expression. Following injection of endotoxin (bacterial lipopolysaccharide) in rats we detected increased inducible NO synthase mRNA levels in the left ventricular wall within 30 min which then peaked at 3 h. This was followed by an increase in myocardial inducible NO synthase enzyme activity and plasma levels of NO metabolites, nitrate and nitrite, which peaked at 6 and 12 h, respectively. The extremely rapid induction of inducible NO synthase may serve to protect the heart against microbial infection and concomitantly alter myocardial mechanical function.</description><identifier>ISSN: 0014-2999</identifier><identifier>EISSN: 1879-0712</identifier><identifier>DOI: 10.1016/0014-2999(96)00099-4</identifier><identifier>PMID: 8804922</identifier><identifier>CODEN: EJPHAZ</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Animal bacterial diseases ; Animals ; Bacterial diseases ; Biological and medical sciences ; Blotting, Northern ; Endotoxin ; Gene Expression ; Heart - drug effects ; Heart, rat ; Infectious diseases ; Lipopolysaccharides - pharmacology ; Male ; Medical sciences ; mRNA ; Myocardium - metabolism ; Nitric oxide (NO) synthase ; Nitric Oxide Synthase - biosynthesis ; Plasma nitrate ; Rats ; Rats, Sprague-Dawley ; RNA, Messenger ; Salmonella typhi ; Septic shock</subject><ispartof>European journal of pharmacology, 1996-05, Vol.303 (1), p.141-144</ispartof><rights>1996</rights><rights>1996 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c483t-94b395d0292c26175609955e65a78bdc93a2934062663013b184c22908b28ff73</citedby><cites>FETCH-LOGICAL-c483t-94b395d0292c26175609955e65a78bdc93a2934062663013b184c22908b28ff73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0014299996000994$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3126192$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8804922$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bateson, Alan N.</creatorcontrib><creatorcontrib>Jakiwczyk, Olga M.</creatorcontrib><creatorcontrib>Schulz, Richard</creatorcontrib><title>Rapid increase in inducible nitric oxide synthase gene expression in the heart during endotoxemia</title><title>European journal of pharmacology</title><addtitle>Eur J Pharmacol</addtitle><description>Inducible, Ca
2+-independent nitric oxide (NO) synthase activity in the heart is elevated during endotoxemia and the resulting excess release of NO depresses cardiac contractile function. We show here that this is due to an extremely rapid induction of inducible NO synthase gene expression. Following injection of endotoxin (bacterial lipopolysaccharide) in rats we detected increased inducible NO synthase mRNA levels in the left ventricular wall within 30 min which then peaked at 3 h. This was followed by an increase in myocardial inducible NO synthase enzyme activity and plasma levels of NO metabolites, nitrate and nitrite, which peaked at 6 and 12 h, respectively. The extremely rapid induction of inducible NO synthase may serve to protect the heart against microbial infection and concomitantly alter myocardial mechanical function.</description><subject>Animal bacterial diseases</subject><subject>Animals</subject><subject>Bacterial diseases</subject><subject>Biological and medical sciences</subject><subject>Blotting, Northern</subject><subject>Endotoxin</subject><subject>Gene Expression</subject><subject>Heart - drug effects</subject><subject>Heart, rat</subject><subject>Infectious diseases</subject><subject>Lipopolysaccharides - pharmacology</subject><subject>Male</subject><subject>Medical sciences</subject><subject>mRNA</subject><subject>Myocardium - metabolism</subject><subject>Nitric oxide (NO) synthase</subject><subject>Nitric Oxide Synthase - biosynthesis</subject><subject>Plasma nitrate</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>RNA, Messenger</subject><subject>Salmonella typhi</subject><subject>Septic shock</subject><issn>0014-2999</issn><issn>1879-0712</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkd9r3DAMx81o6W7d_oMN_FDG-pDOv5JYL4NRtrZQKJTt2Ti20vPIOVc7Gdf_fg533GMHAknoIyF9RchHzq44481XxriqBAB8geaSMQZQqTdkxXULFWu5OCGrI_KWvMv5T4FqEPUZOdOaKRBiReyj3QZPQ3QJbcYSFPOzC92ANIYpBUfHXfBI80uc1gvyhBEp7rYJcw7jwtNpjXSNNk3UzynEJ4rRj9O4w02w78lpb4eMHw7-nPz--ePX9W11_3Bzd_39vnJKy6kC1UmoPRMgnGh4WzfloLrGprat7rwDaQVIxRrRNJJx2XGtnBDAdCd037fynHzez92m8XnGPJlNyA6HwUYc52xaLXmtAf4LFooxIXgB1R50acw5YW-2KWxsejGcmeUFZtHXLPoaWJKysVGl7dNh_txt0B-bDpqX-sWhbrOzQ59sdCEfMcnL-bBg3_YYFtH-Bkwmu4DRoQ8J3WT8GF7f4x_X7aD6</recordid><startdate>19960506</startdate><enddate>19960506</enddate><creator>Bateson, Alan N.</creator><creator>Jakiwczyk, Olga M.</creator><creator>Schulz, Richard</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7X8</scope></search><sort><creationdate>19960506</creationdate><title>Rapid increase in inducible nitric oxide synthase gene expression in the heart during endotoxemia</title><author>Bateson, Alan N. ; Jakiwczyk, Olga M. ; Schulz, Richard</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c483t-94b395d0292c26175609955e65a78bdc93a2934062663013b184c22908b28ff73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Animal bacterial diseases</topic><topic>Animals</topic><topic>Bacterial diseases</topic><topic>Biological and medical sciences</topic><topic>Blotting, Northern</topic><topic>Endotoxin</topic><topic>Gene Expression</topic><topic>Heart - drug effects</topic><topic>Heart, rat</topic><topic>Infectious diseases</topic><topic>Lipopolysaccharides - pharmacology</topic><topic>Male</topic><topic>Medical sciences</topic><topic>mRNA</topic><topic>Myocardium - metabolism</topic><topic>Nitric oxide (NO) synthase</topic><topic>Nitric Oxide Synthase - biosynthesis</topic><topic>Plasma nitrate</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>RNA, Messenger</topic><topic>Salmonella typhi</topic><topic>Septic shock</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bateson, Alan N.</creatorcontrib><creatorcontrib>Jakiwczyk, Olga M.</creatorcontrib><creatorcontrib>Schulz, Richard</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>European journal of pharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bateson, Alan N.</au><au>Jakiwczyk, Olga M.</au><au>Schulz, Richard</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Rapid increase in inducible nitric oxide synthase gene expression in the heart during endotoxemia</atitle><jtitle>European journal of pharmacology</jtitle><addtitle>Eur J Pharmacol</addtitle><date>1996-05-06</date><risdate>1996</risdate><volume>303</volume><issue>1</issue><spage>141</spage><epage>144</epage><pages>141-144</pages><issn>0014-2999</issn><eissn>1879-0712</eissn><coden>EJPHAZ</coden><abstract>Inducible, Ca
2+-independent nitric oxide (NO) synthase activity in the heart is elevated during endotoxemia and the resulting excess release of NO depresses cardiac contractile function. We show here that this is due to an extremely rapid induction of inducible NO synthase gene expression. Following injection of endotoxin (bacterial lipopolysaccharide) in rats we detected increased inducible NO synthase mRNA levels in the left ventricular wall within 30 min which then peaked at 3 h. This was followed by an increase in myocardial inducible NO synthase enzyme activity and plasma levels of NO metabolites, nitrate and nitrite, which peaked at 6 and 12 h, respectively. The extremely rapid induction of inducible NO synthase may serve to protect the heart against microbial infection and concomitantly alter myocardial mechanical function.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>8804922</pmid><doi>10.1016/0014-2999(96)00099-4</doi><tpages>4</tpages></addata></record> |
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| ispartof | European journal of pharmacology, 1996-05, Vol.303 (1), p.141-144 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animal bacterial diseases Animals Bacterial diseases Biological and medical sciences Blotting, Northern Endotoxin Gene Expression Heart - drug effects Heart, rat Infectious diseases Lipopolysaccharides - pharmacology Male Medical sciences mRNA Myocardium - metabolism Nitric oxide (NO) synthase Nitric Oxide Synthase - biosynthesis Plasma nitrate Rats Rats, Sprague-Dawley RNA, Messenger Salmonella typhi Septic shock |
| title | Rapid increase in inducible nitric oxide synthase gene expression in the heart during endotoxemia |
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