Latex allergosorbent test (LAST): A new immunoassay for specific IgE with latex particles
We describe a two-step latex (Lx) agglutination assay for the titration of specific anti- Dermatophagoides pteronyssinus IgE. The samples are first incubated with allergen-coated Lx of 2.3 μm diameter. Bound IgE is digested by pepsin and then titrated by its agglutinating activity on 0.8 μm Lx parti...
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| Veröffentlicht in: | Journal of allergy and clinical immunology 1988-07, Vol.82 (1), p.35-39 |
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| container_title | Journal of allergy and clinical immunology |
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| creator | Gilles, Jean Guy G. Mareschal, Jean-Claude P. Saint-Remy, Jean-Marie R. |
| description | We describe a two-step latex (Lx) agglutination assay for the titration of specific anti-
Dermatophagoides pteronyssinus IgE. The samples are first incubated with allergen-coated Lx of 2.3 μm diameter. Bound IgE is digested by pepsin and then titrated by its agglutinating activity on 0.8 μm Lx particles coated with antihuman Fc
ϵ rabbit F(ab′)
2. This latex allergosorbent test detects 100 pg of specific IgE per milliliter and does not depend on the concentration of total IgE. Owing to a tenfold increase in the allergosorbent surface, no competition with the binding of specific anti-
D. pteronyssinus IgG is observed. Pepsin digestion eliminates potential interferences caused by autoantibodies against IgE. A good correlation (r = 0.92) is found with Phadebas RAST on a series of 91 samples. The latex allergosorbent test does not make use of radioisotopes and can be performed in |
| doi_str_mv | 10.1016/0091-6749(88)90048-6 |
| format | Article |
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Dermatophagoides pteronyssinus IgE. The samples are first incubated with allergen-coated Lx of 2.3 μm diameter. Bound IgE is digested by pepsin and then titrated by its agglutinating activity on 0.8 μm Lx particles coated with antihuman Fc
ϵ rabbit F(ab′)
2. This latex allergosorbent test detects 100 pg of specific IgE per milliliter and does not depend on the concentration of total IgE. Owing to a tenfold increase in the allergosorbent surface, no competition with the binding of specific anti-
D. pteronyssinus IgG is observed. Pepsin digestion eliminates potential interferences caused by autoantibodies against IgE. A good correlation (r = 0.92) is found with Phadebas RAST on a series of 91 samples. The latex allergosorbent test does not make use of radioisotopes and can be performed in <6 hours.</description><identifier>ISSN: 0091-6749</identifier><identifier>EISSN: 1097-6825</identifier><identifier>DOI: 10.1016/0091-6749(88)90048-6</identifier><identifier>PMID: 2455743</identifier><identifier>CODEN: JACIBY</identifier><language>eng</language><publisher>New York, NY: Mosby, Inc</publisher><subject>Animals ; Biological and medical sciences ; Dermatophagoides pteronyssinus ; Epitopes ; Fundamental and applied biological sciences. Psychology ; Fundamental immunology ; Hypersensitivity - diagnosis ; Immediate hypersensitivity. Allergy. Anaphylaxis, etc ; Immunobiology ; Immunoglobulin E - analysis ; Latex Fixation Tests ; Mites - immunology ; Radioallergosorbent Test ; Reference Standards ; Techniques</subject><ispartof>Journal of allergy and clinical immunology, 1988-07, Vol.82 (1), p.35-39</ispartof><rights>1988</rights><rights>1989 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-419b0059c1479a68bb296c71dc9027073d9f36d3b84100319880923633b47f043</citedby><cites>FETCH-LOGICAL-c417t-419b0059c1479a68bb296c71dc9027073d9f36d3b84100319880923633b47f043</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0091-6749(88)90048-6$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=6994413$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2455743$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gilles, Jean Guy G.</creatorcontrib><creatorcontrib>Mareschal, Jean-Claude P.</creatorcontrib><creatorcontrib>Saint-Remy, Jean-Marie R.</creatorcontrib><title>Latex allergosorbent test (LAST): A new immunoassay for specific IgE with latex particles</title><title>Journal of allergy and clinical immunology</title><addtitle>J Allergy Clin Immunol</addtitle><description>We describe a two-step latex (Lx) agglutination assay for the titration of specific anti-
Dermatophagoides pteronyssinus IgE. The samples are first incubated with allergen-coated Lx of 2.3 μm diameter. Bound IgE is digested by pepsin and then titrated by its agglutinating activity on 0.8 μm Lx particles coated with antihuman Fc
ϵ rabbit F(ab′)
2. This latex allergosorbent test detects 100 pg of specific IgE per milliliter and does not depend on the concentration of total IgE. Owing to a tenfold increase in the allergosorbent surface, no competition with the binding of specific anti-
D. pteronyssinus IgG is observed. Pepsin digestion eliminates potential interferences caused by autoantibodies against IgE. A good correlation (r = 0.92) is found with Phadebas RAST on a series of 91 samples. The latex allergosorbent test does not make use of radioisotopes and can be performed in <6 hours.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Dermatophagoides pteronyssinus</subject><subject>Epitopes</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>Hypersensitivity - diagnosis</subject><subject>Immediate hypersensitivity. Allergy. Anaphylaxis, etc</subject><subject>Immunobiology</subject><subject>Immunoglobulin E - analysis</subject><subject>Latex Fixation Tests</subject><subject>Mites - immunology</subject><subject>Radioallergosorbent Test</subject><subject>Reference Standards</subject><subject>Techniques</subject><issn>0091-6749</issn><issn>1097-6825</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1988</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1rFEEQhhtR4pr4DxT6IJIcJqma7ukPD8ISEg0seDAePDU9PTWxZT7W7llj_n1ms8sezako6nlfioexdwjnCKguACwWSkt7asyZBZCmUC_YAsHqQpmyeskWB-Q1e5Pzb5h3YewROyplVWkpFuznyk_0j_uuo3Q35jHVNEx8ojzx09Xy--3ZJ77kA93z2PebYfQ5-wfejonnNYXYxsBv7q74fZx-8e6pae3TFENH-YS9an2X6e1-HrMf11e3l1-L1bcvN5fLVREk6qmQaGuAygaU2npl6rq0KmhsgoVSgxaNbYVqRG0kAgi0xoAthRKilroFKY7Zx13vOo1_NvPjro85UNf5gcZNdtoIrIyCZ0GsALBSOINyB4Y05pyodesUe58eHILbqndbr27r1RnjntQ7Ncfe7_s3dU_NIbR3Pd8_7O8-B9-1yQ8h5gOmrJUSt9jnHUaztL-Rkssh0hCoiYnC5Jox_v-PR5pfnJQ</recordid><startdate>19880701</startdate><enddate>19880701</enddate><creator>Gilles, Jean Guy G.</creator><creator>Mareschal, Jean-Claude P.</creator><creator>Saint-Remy, Jean-Marie R.</creator><general>Mosby, Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19880701</creationdate><title>Latex allergosorbent test (LAST): A new immunoassay for specific IgE with latex particles</title><author>Gilles, Jean Guy G. ; Mareschal, Jean-Claude P. ; Saint-Remy, Jean-Marie R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-419b0059c1479a68bb296c71dc9027073d9f36d3b84100319880923633b47f043</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1988</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Dermatophagoides pteronyssinus</topic><topic>Epitopes</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fundamental immunology</topic><topic>Hypersensitivity - diagnosis</topic><topic>Immediate hypersensitivity. Allergy. Anaphylaxis, etc</topic><topic>Immunobiology</topic><topic>Immunoglobulin E - analysis</topic><topic>Latex Fixation Tests</topic><topic>Mites - immunology</topic><topic>Radioallergosorbent Test</topic><topic>Reference Standards</topic><topic>Techniques</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gilles, Jean Guy G.</creatorcontrib><creatorcontrib>Mareschal, Jean-Claude P.</creatorcontrib><creatorcontrib>Saint-Remy, Jean-Marie R.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of allergy and clinical immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gilles, Jean Guy G.</au><au>Mareschal, Jean-Claude P.</au><au>Saint-Remy, Jean-Marie R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Latex allergosorbent test (LAST): A new immunoassay for specific IgE with latex particles</atitle><jtitle>Journal of allergy and clinical immunology</jtitle><addtitle>J Allergy Clin Immunol</addtitle><date>1988-07-01</date><risdate>1988</risdate><volume>82</volume><issue>1</issue><spage>35</spage><epage>39</epage><pages>35-39</pages><issn>0091-6749</issn><eissn>1097-6825</eissn><coden>JACIBY</coden><abstract>We describe a two-step latex (Lx) agglutination assay for the titration of specific anti-
Dermatophagoides pteronyssinus IgE. The samples are first incubated with allergen-coated Lx of 2.3 μm diameter. Bound IgE is digested by pepsin and then titrated by its agglutinating activity on 0.8 μm Lx particles coated with antihuman Fc
ϵ rabbit F(ab′)
2. This latex allergosorbent test detects 100 pg of specific IgE per milliliter and does not depend on the concentration of total IgE. Owing to a tenfold increase in the allergosorbent surface, no competition with the binding of specific anti-
D. pteronyssinus IgG is observed. Pepsin digestion eliminates potential interferences caused by autoantibodies against IgE. A good correlation (r = 0.92) is found with Phadebas RAST on a series of 91 samples. The latex allergosorbent test does not make use of radioisotopes and can be performed in <6 hours.</abstract><cop>New York, NY</cop><pub>Mosby, Inc</pub><pmid>2455743</pmid><doi>10.1016/0091-6749(88)90048-6</doi><tpages>5</tpages></addata></record> |
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| ispartof | Journal of allergy and clinical immunology, 1988-07, Vol.82 (1), p.35-39 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete; EZB-FREE-00999 freely available EZB journals |
| subjects | Animals Biological and medical sciences Dermatophagoides pteronyssinus Epitopes Fundamental and applied biological sciences. Psychology Fundamental immunology Hypersensitivity - diagnosis Immediate hypersensitivity. Allergy. Anaphylaxis, etc Immunobiology Immunoglobulin E - analysis Latex Fixation Tests Mites - immunology Radioallergosorbent Test Reference Standards Techniques |
| title | Latex allergosorbent test (LAST): A new immunoassay for specific IgE with latex particles |
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