Preparation of a cell-free translation system from a wild-type strain of Neurospora crassa
We describe the preparation of an in vitro translation system from a wild-type strain of Neurospora crassa. The system is capable of supporting efficient and faithful translation of native and in vitro transcribed eukaryotic messages. The translation products have minimal background and can be clear...
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| Veröffentlicht in: | Current genetics 1988-04, Vol.13 (4), p.323-326 |
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| container_title | Current genetics |
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| creator | DEVCHAND, M BUXTON, F. P GWYNNE, D. I DAVIES, R. W |
| description | We describe the preparation of an in vitro translation system from a wild-type strain of Neurospora crassa. The system is capable of supporting efficient and faithful translation of native and in vitro transcribed eukaryotic messages. The translation products have minimal background and can be clearly analyzed by SDS-polyacrylamide gel electrophoresis. The method of preparation of the lysate is simple, fast and reproducible. The procedure should be readily applicable to other filamentous fungi. |
| doi_str_mv | 10.1007/BF00424426 |
| format | Article |
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The procedure should be readily applicable to other filamentous fungi.</description><identifier>ISSN: 0172-8083</identifier><identifier>EISSN: 1432-0983</identifier><identifier>DOI: 10.1007/BF00424426</identifier><identifier>PMID: 2968852</identifier><identifier>CODEN: CUGED5</identifier><language>eng</language><publisher>Heidelberg: Springer</publisher><subject>Biological and medical sciences ; Cell-Free System ; Fundamental and applied biological sciences. Psychology ; Kinetics ; Magnesium - pharmacology ; Molecular and cellular biology ; Molecular genetics ; Neurospora - genetics ; Neurospora crassa ; Neurospora crassa - genetics ; Potassium - pharmacology ; Protein Biosynthesis - drug effects ; RNA, Messenger - genetics ; Transcription, Genetic ; Translation. Translation factors. 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P</creatorcontrib><creatorcontrib>GWYNNE, D. I</creatorcontrib><creatorcontrib>DAVIES, R. W</creatorcontrib><title>Preparation of a cell-free translation system from a wild-type strain of Neurospora crassa</title><title>Current genetics</title><addtitle>Curr Genet</addtitle><description>We describe the preparation of an in vitro translation system from a wild-type strain of Neurospora crassa. The system is capable of supporting efficient and faithful translation of native and in vitro transcribed eukaryotic messages. The translation products have minimal background and can be clearly analyzed by SDS-polyacrylamide gel electrophoresis. The method of preparation of the lysate is simple, fast and reproducible. The procedure should be readily applicable to other filamentous fungi.</description><subject>Biological and medical sciences</subject><subject>Cell-Free System</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Kinetics</subject><subject>Magnesium - pharmacology</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Neurospora - genetics</subject><subject>Neurospora crassa</subject><subject>Neurospora crassa - genetics</subject><subject>Potassium - pharmacology</subject><subject>Protein Biosynthesis - drug effects</subject><subject>RNA, Messenger - genetics</subject><subject>Transcription, Genetic</subject><subject>Translation. Translation factors. 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Psychology</topic><topic>Kinetics</topic><topic>Magnesium - pharmacology</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Neurospora - genetics</topic><topic>Neurospora crassa</topic><topic>Neurospora crassa - genetics</topic><topic>Potassium - pharmacology</topic><topic>Protein Biosynthesis - drug effects</topic><topic>RNA, Messenger - genetics</topic><topic>Transcription, Genetic</topic><topic>Translation. Translation factors. Protein processing</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>DEVCHAND, M</creatorcontrib><creatorcontrib>BUXTON, F. P</creatorcontrib><creatorcontrib>GWYNNE, D. I</creatorcontrib><creatorcontrib>DAVIES, R. 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W</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Preparation of a cell-free translation system from a wild-type strain of Neurospora crassa</atitle><jtitle>Current genetics</jtitle><addtitle>Curr Genet</addtitle><date>1988-04-01</date><risdate>1988</risdate><volume>13</volume><issue>4</issue><spage>323</spage><epage>326</epage><pages>323-326</pages><issn>0172-8083</issn><eissn>1432-0983</eissn><coden>CUGED5</coden><abstract>We describe the preparation of an in vitro translation system from a wild-type strain of Neurospora crassa. The system is capable of supporting efficient and faithful translation of native and in vitro transcribed eukaryotic messages. The translation products have minimal background and can be clearly analyzed by SDS-polyacrylamide gel electrophoresis. The method of preparation of the lysate is simple, fast and reproducible. The procedure should be readily applicable to other filamentous fungi.</abstract><cop>Heidelberg</cop><cop>Berlin</cop><pub>Springer</pub><pmid>2968852</pmid><doi>10.1007/BF00424426</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0172-8083 |
| ispartof | Current genetics, 1988-04, Vol.13 (4), p.323-326 |
| issn | 0172-8083 1432-0983 |
| language | eng |
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| source | MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | Biological and medical sciences Cell-Free System Fundamental and applied biological sciences. Psychology Kinetics Magnesium - pharmacology Molecular and cellular biology Molecular genetics Neurospora - genetics Neurospora crassa Neurospora crassa - genetics Potassium - pharmacology Protein Biosynthesis - drug effects RNA, Messenger - genetics Transcription, Genetic Translation. Translation factors. Protein processing |
| title | Preparation of a cell-free translation system from a wild-type strain of Neurospora crassa |
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