L-arginine is the physiological precursor for the formation of nitric oxide in endothelium-dependent relaxation
The formation of nitric oxide (NO) from L-arginine by vascular endothelial cells and its relationship to endothelium-dependent relaxation of vascular rings was studied. The release of NO, measured by bioassay or chemiluminescence, from porcine aortic endothelial cells stimulated with bradykinin was...
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| Veröffentlicht in: | Biochemical and biophysical research communications 1988-06, Vol.153 (3), p.1251-1256 |
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| container_title | Biochemical and biophysical research communications |
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| creator | Palmer, Richard M.J. Rees, Daryl D. Ashton, David S. Moncada, Salvador |
| description | The formation of nitric oxide (NO) from L-arginine by vascular endothelial cells and its relationship to endothelium-dependent relaxation of vascular rings was studied. The release of NO, measured by bioassay or chemiluminescence, from porcine aortic endothelial cells stimulated with bradykinin was enhanced by infusions of L-, but not D-arginine. The release of
15NO, determined by high resolution mass spectrometry, from L-guanidino
15N (99%) arginine was also observed, indicating that NO is formed from the terminal guanidino nitrogen atom(s) of L-arginine. L-N
G-monomethyl arginine (L-NMMA), but not D-NMMA, inhibited both the generation of NO by endothelial cells in culture and the endothelium-dependent relaxation of rabbit aortic rings. Both these effects were reversed by L-arginine. These data indicate that L-arginine is the physiological precursor for the formation of NO which mediates endothelium-dependent relaxation. |
| doi_str_mv | 10.1016/S0006-291X(88)81362-7 |
| format | Article |
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15NO, determined by high resolution mass spectrometry, from L-guanidino
15N (99%) arginine was also observed, indicating that NO is formed from the terminal guanidino nitrogen atom(s) of L-arginine. L-N
G-monomethyl arginine (L-NMMA), but not D-NMMA, inhibited both the generation of NO by endothelial cells in culture and the endothelium-dependent relaxation of rabbit aortic rings. Both these effects were reversed by L-arginine. These data indicate that L-arginine is the physiological precursor for the formation of NO which mediates endothelium-dependent relaxation.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/S0006-291X(88)81362-7</identifier><identifier>PMID: 3390182</identifier><identifier>CODEN: BBRCA9</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>Animals ; Arginine - metabolism ; Biological and medical sciences ; Biological Assay ; Bradykinin - pharmacology ; Endothelium, Vascular - drug effects ; Endothelium, Vascular - metabolism ; Fundamental and applied biological sciences. Psychology ; Isomerism ; Luminescent Measurements ; Nitric Oxide - metabolism ; Swine ; Vasodilation ; Vertebrates: blood, hematopoietic organs, reticuloendothelial system</subject><ispartof>Biochemical and biophysical research communications, 1988-06, Vol.153 (3), p.1251-1256</ispartof><rights>1988 Academic Press, Inc.</rights><rights>1989 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c530t-4ad2a631df4ef47c5c917e916e0d2cb3877097c0e70b6686952b8c1df09756d3</citedby><cites>FETCH-LOGICAL-c530t-4ad2a631df4ef47c5c917e916e0d2cb3877097c0e70b6686952b8c1df09756d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0006-291X(88)81362-7$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27923,27924,45994</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7331241$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3390182$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Palmer, Richard M.J.</creatorcontrib><creatorcontrib>Rees, Daryl D.</creatorcontrib><creatorcontrib>Ashton, David S.</creatorcontrib><creatorcontrib>Moncada, Salvador</creatorcontrib><title>L-arginine is the physiological precursor for the formation of nitric oxide in endothelium-dependent relaxation</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>The formation of nitric oxide (NO) from L-arginine by vascular endothelial cells and its relationship to endothelium-dependent relaxation of vascular rings was studied. The release of NO, measured by bioassay or chemiluminescence, from porcine aortic endothelial cells stimulated with bradykinin was enhanced by infusions of L-, but not D-arginine. The release of
15NO, determined by high resolution mass spectrometry, from L-guanidino
15N (99%) arginine was also observed, indicating that NO is formed from the terminal guanidino nitrogen atom(s) of L-arginine. L-N
G-monomethyl arginine (L-NMMA), but not D-NMMA, inhibited both the generation of NO by endothelial cells in culture and the endothelium-dependent relaxation of rabbit aortic rings. Both these effects were reversed by L-arginine. These data indicate that L-arginine is the physiological precursor for the formation of NO which mediates endothelium-dependent relaxation.</description><subject>Animals</subject><subject>Arginine - metabolism</subject><subject>Biological and medical sciences</subject><subject>Biological Assay</subject><subject>Bradykinin - pharmacology</subject><subject>Endothelium, Vascular - drug effects</subject><subject>Endothelium, Vascular - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Isomerism</subject><subject>Luminescent Measurements</subject><subject>Nitric Oxide - metabolism</subject><subject>Swine</subject><subject>Vasodilation</subject><subject>Vertebrates: blood, hematopoietic organs, reticuloendothelial system</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1988</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE2PFCEQhonRrLOrP2ETDsa4h9YCuoE-GbPxK5nEg3vwRhio3sV0QwvdZvffy3xkrh5IBd6nqshDyDWD9wyY_PATAGTDe_brndY3mgnJG_WMbBj00HAG7XOyOSMvyWUpvwEYa2V_QS6E6IFpviFp29h8H2KISEOhywPS-eGphDSm--DsSOeMbs0lZTrUs89rnewSUqRpoDEsOTiaHoOvAyLF6FOFxrBOjce5XjEuNONoHw89r8iLwY4FX5_qFbn78vnu9luz_fH1--2nbeM6AUvTWs-tFMwPLQ6tcp3rmcKeSQTP3U5opaBXDlDBTkot-47vtKt4fe2kF1fk7XHsnNOfFctiplAcjqONmNZilBbQctZVsDuCLqdSMg5mzmGy-ckwMHvP5uDZ7CUarc3Bs1G17_q0YN1N6M9dJ7E1f3PKbakah2yjC-WMKSEYb1nFPh4xrC7-BsymuIDRoQ_V-2J8Cv_5yD__zpvR</recordid><startdate>19880630</startdate><enddate>19880630</enddate><creator>Palmer, Richard M.J.</creator><creator>Rees, Daryl D.</creator><creator>Ashton, David S.</creator><creator>Moncada, Salvador</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19880630</creationdate><title>L-arginine is the physiological precursor for the formation of nitric oxide in endothelium-dependent relaxation</title><author>Palmer, Richard M.J. ; Rees, Daryl D. ; Ashton, David S. ; Moncada, Salvador</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c530t-4ad2a631df4ef47c5c917e916e0d2cb3877097c0e70b6686952b8c1df09756d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1988</creationdate><topic>Animals</topic><topic>Arginine - metabolism</topic><topic>Biological and medical sciences</topic><topic>Biological Assay</topic><topic>Bradykinin - pharmacology</topic><topic>Endothelium, Vascular - drug effects</topic><topic>Endothelium, Vascular - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Isomerism</topic><topic>Luminescent Measurements</topic><topic>Nitric Oxide - metabolism</topic><topic>Swine</topic><topic>Vasodilation</topic><topic>Vertebrates: blood, hematopoietic organs, reticuloendothelial system</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Palmer, Richard M.J.</creatorcontrib><creatorcontrib>Rees, Daryl D.</creatorcontrib><creatorcontrib>Ashton, David S.</creatorcontrib><creatorcontrib>Moncada, Salvador</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Palmer, Richard M.J.</au><au>Rees, Daryl D.</au><au>Ashton, David S.</au><au>Moncada, Salvador</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>L-arginine is the physiological precursor for the formation of nitric oxide in endothelium-dependent relaxation</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1988-06-30</date><risdate>1988</risdate><volume>153</volume><issue>3</issue><spage>1251</spage><epage>1256</epage><pages>1251-1256</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><coden>BBRCA9</coden><abstract>The formation of nitric oxide (NO) from L-arginine by vascular endothelial cells and its relationship to endothelium-dependent relaxation of vascular rings was studied. The release of NO, measured by bioassay or chemiluminescence, from porcine aortic endothelial cells stimulated with bradykinin was enhanced by infusions of L-, but not D-arginine. The release of
15NO, determined by high resolution mass spectrometry, from L-guanidino
15N (99%) arginine was also observed, indicating that NO is formed from the terminal guanidino nitrogen atom(s) of L-arginine. L-N
G-monomethyl arginine (L-NMMA), but not D-NMMA, inhibited both the generation of NO by endothelial cells in culture and the endothelium-dependent relaxation of rabbit aortic rings. Both these effects were reversed by L-arginine. These data indicate that L-arginine is the physiological precursor for the formation of NO which mediates endothelium-dependent relaxation.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>3390182</pmid><doi>10.1016/S0006-291X(88)81362-7</doi><tpages>6</tpages></addata></record> |
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| ispartof | Biochemical and biophysical research communications, 1988-06, Vol.153 (3), p.1251-1256 |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Animals Arginine - metabolism Biological and medical sciences Biological Assay Bradykinin - pharmacology Endothelium, Vascular - drug effects Endothelium, Vascular - metabolism Fundamental and applied biological sciences. Psychology Isomerism Luminescent Measurements Nitric Oxide - metabolism Swine Vasodilation Vertebrates: blood, hematopoietic organs, reticuloendothelial system |
| title | L-arginine is the physiological precursor for the formation of nitric oxide in endothelium-dependent relaxation |
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