[4] Purification of functional recombinant P450s from bacteria

This chapter discusses the methods for the purification of functional recombinant P450s from bacteria. A number of expression systems have been utilized to produce recombinant P450s, as discussed in this and an earlier volume. I Bacteria have considerable advantages because of the high levels of exp...

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Veröffentlicht in:	Methods in Enzymology 1996, Vol.272, p.35-44
Hauptverfasser:	Guengerich, F. Peter, Martin, Martha V., Guo, Zuyu, Chun, Young-Jin
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Animals Chromatography, Ion Exchange Cytochrome P-450 CYP2D6 - biosynthesis Cytochrome P-450 CYP2D6 - genetics Cytochrome P-450 CYP2D6 - isolation & purification Cytochrome P-450 Enzyme System - biosynthesis Cytochrome P-450 Enzyme System - genetics Cytochrome P-450 Enzyme System - isolation & purification Escherichia coli - enzymology Escherichia coli - genetics Humans Molecular Sequence Data NADPH-Ferrihemoprotein Reductase - biosynthesis NADPH-Ferrihemoprotein Reductase - genetics NADPH-Ferrihemoprotein Reductase - isolation & purification Plasmids - genetics Rats Recombinant Fusion Proteins - biosynthesis Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - isolation & purification Recombinant Proteins - biosynthesis Recombinant Proteins - genetics Recombinant Proteins - isolation & purification Salmonella typhimurium - enzymology Salmonella typhimurium - genetics
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