[6] Yeast expression of animal and plant P450s in optimized redox environments

Yeast Saccharomyces cerevisiae offers a low-cost and efficient way to express heterologous P450s. The limiting amounts of endogenous P450-reductase (CPR) present in this organism and the limited sequence similarity of yeast redox enzymes (CPR and cytochrome b5) with human liver or plant equivalents...

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Veröffentlicht in:	Methods in Enzymology 1996, Vol.272, p.51-64
Hauptverfasser:	Pompon, Denis, Louerat, Benedicte, Bronine, Alexis, Urban, Philippe
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Cinnamates - metabolism Cloning, Molecular Cytochrome P-450 Enzyme System - genetics Cytochrome P-450 Enzyme System - isolation & purification Cytochrome P-450 Enzyme System - metabolism DNA, Complementary - genetics Gene Expression Genetic Engineering Genetic Vectors Humans Mixed Function Oxygenases - genetics Mixed Function Oxygenases - metabolism Oxidation-Reduction Plants - enzymology Plants - genetics Recombinant Proteins - genetics Recombinant Proteins - isolation & purification Recombinant Proteins - metabolism Saccharomyces cerevisiae - enzymology Saccharomyces cerevisiae - genetics Trans-Cinnamate 4-Monooxygenase Transformation, Genetic
Online-Zugang:	Volltext
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