Fluorometric Determination for Micro Amounts of Albumin and Globulin Fractions without Separation by Using α,β,γ,δ-Tetra(4′-carboxyphenyl)porphin

Fluorometric Determination for Micro Amounts of Albumin and Globulin Fractions without Separation by Using α,β,γ,δ-Tetra(4′-carboxyphenyl)porphin

A simultaneous determination of micro amounts of albumin and globulin fractions by fluorometry with only reagent α,β,γ,δ-tetra(4′-carboxyphenyl)porphin (TCPP) in the aqueous solution has been developed. Under the experimental conditions (5.6 × 10−7mol/liter of TCPP, pH 3.0, and at room temperature),...

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Veröffentlicht in:Analytical biochemistry 1996-01, Vol.233 (2), p.151-155
Hauptverfasser: Li, Na, Li, Ke-An, Tong, Shen-Yang
Format: Artikel
Sprache:eng
Schlagworte:
Globulins - analysis
Hot Temperature
Humans
Porphyrins - chemistry
Serum Albumin - analysis
Sodium Dodecyl Sulfate
Spectrometry, Fluorescence
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container_title Analytical biochemistry
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creator Li, Na
Li, Ke-An
Tong, Shen-Yang
description A simultaneous determination of micro amounts of albumin and globulin fractions by fluorometry with only reagent α,β,γ,δ-tetra(4′-carboxyphenyl)porphin (TCPP) in the aqueous solution has been developed. Under the experimental conditions (5.6 × 10−7mol/liter of TCPP, pH 3.0, and at room temperature), the linear range of this assay was 0.09–12 μg/ml and the detection limits (3S/N) were 60 ng/ml for albumin and 90 ng/ml for globulin, respectively. The human serum samples were measured satisfactorily by using this method.
doi_str_mv 10.1006/abio.1996.0022
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subjects Globulins - analysis
Hot Temperature
Humans
Porphyrins - chemistry
Serum Albumin - analysis
Sodium Dodecyl Sulfate
Spectrometry, Fluorescence
title Fluorometric Determination for Micro Amounts of Albumin and Globulin Fractions without Separation by Using α,β,γ,δ-Tetra(4′-carboxyphenyl)porphin
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