Radioiodination of proteins and lipoproteins using N-bromosuccinimide as oxidizing agent
In an attempt to improve conditions for radioiodination of sensitive proteins we used N-bromosuccinimide as a mild oxidizing agent. Under gentle conditions we increased the average labeling efficiency of a wide variety of proteins to above 97%. There was no loss of binding activity of low density li...
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| Veröffentlicht in: | Anal. Biochem.; (United States) 1988-04, Vol.170 (1), p.186-192 |
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| container_title | Anal. Biochem.; (United States) |
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| creator | Sinn, H.J. Schrenk, H.H. Friedrich, E.A. Via, D.P. Dresel, H.A. |
| description | In an attempt to improve conditions for radioiodination of sensitive proteins we used
N-bromosuccinimide as a mild oxidizing agent. Under gentle conditions we increased the average labeling efficiency of a wide variety of proteins to above 97%. There was no loss of binding activity of low density lipoprotein particles, which are most sensitive to oxidation. Depending on high labeling efficiency, our method reduces preparation time as well as radioactive waste, costs, and irradiation exposure to personnel. |
| doi_str_mv | 10.1016/0003-2697(88)90107-8 |
| format | Article |
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N-bromosuccinimide as a mild oxidizing agent. Under gentle conditions we increased the average labeling efficiency of a wide variety of proteins to above 97%. There was no loss of binding activity of low density lipoprotein particles, which are most sensitive to oxidation. Depending on high labeling efficiency, our method reduces preparation time as well as radioactive waste, costs, and irradiation exposure to personnel.</description><identifier>ISSN: 0003-2697</identifier><identifier>EISSN: 1096-0309</identifier><identifier>DOI: 10.1016/0003-2697(88)90107-8</identifier><identifier>PMID: 3389510</identifier><identifier>CODEN: ANBCA2</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>Analytical, structural and metabolic biochemistry ; ANIMALS ; BASIC BIOLOGICAL SCIENCES ; Biological and medical sciences ; Bromosuccinimide - pharmacology ; CARBOXYLIC ACIDS ; CATTLE ; CHEMICAL REACTIONS ; DICARBOXYLIC ACIDS ; DOMESTIC ANIMALS ; Fundamental and applied biological sciences. Psychology ; General aspects, investigation methods ; IODINE ISOTOPES ; Iodine Radioisotopes ; ISOTOPE APPLICATIONS ; Isotope Labeling - methods ; ISOTOPES ; LABELLING ; LIPIDS ; LIPOPROTEINS ; low density lipoproteins ; MAMMALS ; N-bromosuccinimide ; ORGANIC ACIDS ; ORGANIC COMPOUNDS ; Oxidation-Reduction ; OXIDIZERS ; PROTEINS ; radioiodination ; REDOX REACTIONS ; RUMINANTS ; Serum Albumin, Bovine ; SUCCINIC ACID ; Succinimides - pharmacology ; TRACER TECHNIQUES ; VERTEBRATES 550201 -- Biochemistry-- Tracer Techniques</subject><ispartof>Anal. Biochem.; (United States), 1988-04, Vol.170 (1), p.186-192</ispartof><rights>1988</rights><rights>1989 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c510t-b5e47a8181cde3c716d639cae5b8d6061267ceb1e7051162b2911e5c10cb8fc33</citedby><cites>FETCH-LOGICAL-c510t-b5e47a8181cde3c716d639cae5b8d6061267ceb1e7051162b2911e5c10cb8fc33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0003-2697(88)90107-8$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,781,785,886,3551,27929,27930,46000</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7238957$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3389510$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/6829133$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Sinn, H.J.</creatorcontrib><creatorcontrib>Schrenk, H.H.</creatorcontrib><creatorcontrib>Friedrich, E.A.</creatorcontrib><creatorcontrib>Via, D.P.</creatorcontrib><creatorcontrib>Dresel, H.A.</creatorcontrib><creatorcontrib>Deutsches Krebsforschungszentrum, Heidelberg (Germany, F.R.)</creatorcontrib><title>Radioiodination of proteins and lipoproteins using N-bromosuccinimide as oxidizing agent</title><title>Anal. Biochem.; (United States)</title><addtitle>Anal Biochem</addtitle><description>In an attempt to improve conditions for radioiodination of sensitive proteins we used
N-bromosuccinimide as a mild oxidizing agent. Under gentle conditions we increased the average labeling efficiency of a wide variety of proteins to above 97%. There was no loss of binding activity of low density lipoprotein particles, which are most sensitive to oxidation. Depending on high labeling efficiency, our method reduces preparation time as well as radioactive waste, costs, and irradiation exposure to personnel.</description><subject>Analytical, structural and metabolic biochemistry</subject><subject>ANIMALS</subject><subject>BASIC BIOLOGICAL SCIENCES</subject><subject>Biological and medical sciences</subject><subject>Bromosuccinimide - pharmacology</subject><subject>CARBOXYLIC ACIDS</subject><subject>CATTLE</subject><subject>CHEMICAL REACTIONS</subject><subject>DICARBOXYLIC ACIDS</subject><subject>DOMESTIC ANIMALS</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General aspects, investigation methods</subject><subject>IODINE ISOTOPES</subject><subject>Iodine Radioisotopes</subject><subject>ISOTOPE APPLICATIONS</subject><subject>Isotope Labeling - methods</subject><subject>ISOTOPES</subject><subject>LABELLING</subject><subject>LIPIDS</subject><subject>LIPOPROTEINS</subject><subject>low density lipoproteins</subject><subject>MAMMALS</subject><subject>N-bromosuccinimide</subject><subject>ORGANIC ACIDS</subject><subject>ORGANIC COMPOUNDS</subject><subject>Oxidation-Reduction</subject><subject>OXIDIZERS</subject><subject>PROTEINS</subject><subject>radioiodination</subject><subject>REDOX REACTIONS</subject><subject>RUMINANTS</subject><subject>Serum Albumin, Bovine</subject><subject>SUCCINIC ACID</subject><subject>Succinimides - pharmacology</subject><subject>TRACER TECHNIQUES</subject><subject>VERTEBRATES 550201 -- Biochemistry-- Tracer Techniques</subject><issn>0003-2697</issn><issn>1096-0309</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1988</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkV2L1TAQhoMo69nVf6BQREQvqpl-pMmNIMv6AYuCKHgX0sl0HWmTY9OK-utNPYdzqVeBeZ-ZzDuvEA9APgcJ6oWUsi4rZbqnWj8zEmRX6ltiB9KoUtbS3Ba7E3JXnKf0TUqAplVn4qyutWlB7sSXj85z5Og5uIVjKOJQ7Oe4EIdUuOCLkffxVFgTh5vifdnPcYppReTAE3sqXCriT_b8e9PdDYXlnrgzuDHR_eN7IT6_vvp0-ba8_vDm3eWr6xLz_0vZt9R0ToMG9FRjB8qr2qCjttdeSQWV6pB6oE62AKrqKwNALYLEXg9Y1xfi0WFuTAvbhLwQfsUYAuFilc54vUFPDlB28n2ltNiJE9I4ukBxTbbLnKm66r8gNKaVbaMy2BxAnGNKMw12P_Pk5l8WpN3isdvt7XZ7q7X9G4_Vue3hcf7aT-RPTcc8sv74qLuEbhxmF5DTCcsbZq7L2MsDRvmyP5jmzTgFJM_z5ttH_vcefwBCZquG</recordid><startdate>19880401</startdate><enddate>19880401</enddate><creator>Sinn, H.J.</creator><creator>Schrenk, H.H.</creator><creator>Friedrich, E.A.</creator><creator>Via, D.P.</creator><creator>Dresel, H.A.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope><scope>7X8</scope><scope>OTOTI</scope></search><sort><creationdate>19880401</creationdate><title>Radioiodination of proteins and lipoproteins using N-bromosuccinimide as oxidizing agent</title><author>Sinn, H.J. ; Schrenk, H.H. ; Friedrich, E.A. ; Via, D.P. ; Dresel, H.A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c510t-b5e47a8181cde3c716d639cae5b8d6061267ceb1e7051162b2911e5c10cb8fc33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1988</creationdate><topic>Analytical, structural and metabolic biochemistry</topic><topic>ANIMALS</topic><topic>BASIC BIOLOGICAL SCIENCES</topic><topic>Biological and medical sciences</topic><topic>Bromosuccinimide - pharmacology</topic><topic>CARBOXYLIC ACIDS</topic><topic>CATTLE</topic><topic>CHEMICAL REACTIONS</topic><topic>DICARBOXYLIC ACIDS</topic><topic>DOMESTIC ANIMALS</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General aspects, investigation methods</topic><topic>IODINE ISOTOPES</topic><topic>Iodine Radioisotopes</topic><topic>ISOTOPE APPLICATIONS</topic><topic>Isotope Labeling - methods</topic><topic>ISOTOPES</topic><topic>LABELLING</topic><topic>LIPIDS</topic><topic>LIPOPROTEINS</topic><topic>low density lipoproteins</topic><topic>MAMMALS</topic><topic>N-bromosuccinimide</topic><topic>ORGANIC ACIDS</topic><topic>ORGANIC COMPOUNDS</topic><topic>Oxidation-Reduction</topic><topic>OXIDIZERS</topic><topic>PROTEINS</topic><topic>radioiodination</topic><topic>REDOX REACTIONS</topic><topic>RUMINANTS</topic><topic>Serum Albumin, Bovine</topic><topic>SUCCINIC ACID</topic><topic>Succinimides - pharmacology</topic><topic>TRACER TECHNIQUES</topic><topic>VERTEBRATES 550201 -- Biochemistry-- Tracer Techniques</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sinn, H.J.</creatorcontrib><creatorcontrib>Schrenk, H.H.</creatorcontrib><creatorcontrib>Friedrich, E.A.</creatorcontrib><creatorcontrib>Via, D.P.</creatorcontrib><creatorcontrib>Dresel, H.A.</creatorcontrib><creatorcontrib>Deutsches Krebsforschungszentrum, Heidelberg (Germany, F.R.)</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV</collection><jtitle>Anal. Biochem.; (United States)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sinn, H.J.</au><au>Schrenk, H.H.</au><au>Friedrich, E.A.</au><au>Via, D.P.</au><au>Dresel, H.A.</au><aucorp>Deutsches Krebsforschungszentrum, Heidelberg (Germany, F.R.)</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Radioiodination of proteins and lipoproteins using N-bromosuccinimide as oxidizing agent</atitle><jtitle>Anal. Biochem.; (United States)</jtitle><addtitle>Anal Biochem</addtitle><date>1988-04-01</date><risdate>1988</risdate><volume>170</volume><issue>1</issue><spage>186</spage><epage>192</epage><pages>186-192</pages><issn>0003-2697</issn><eissn>1096-0309</eissn><coden>ANBCA2</coden><abstract>In an attempt to improve conditions for radioiodination of sensitive proteins we used
N-bromosuccinimide as a mild oxidizing agent. Under gentle conditions we increased the average labeling efficiency of a wide variety of proteins to above 97%. There was no loss of binding activity of low density lipoprotein particles, which are most sensitive to oxidation. Depending on high labeling efficiency, our method reduces preparation time as well as radioactive waste, costs, and irradiation exposure to personnel.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>3389510</pmid><doi>10.1016/0003-2697(88)90107-8</doi><tpages>7</tpages></addata></record> |
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| ispartof | Anal. Biochem.; (United States), 1988-04, Vol.170 (1), p.186-192 |
| issn | 0003-2697 1096-0309 |
| language | eng |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Analytical, structural and metabolic biochemistry ANIMALS BASIC BIOLOGICAL SCIENCES Biological and medical sciences Bromosuccinimide - pharmacology CARBOXYLIC ACIDS CATTLE CHEMICAL REACTIONS DICARBOXYLIC ACIDS DOMESTIC ANIMALS Fundamental and applied biological sciences. Psychology General aspects, investigation methods IODINE ISOTOPES Iodine Radioisotopes ISOTOPE APPLICATIONS Isotope Labeling - methods ISOTOPES LABELLING LIPIDS LIPOPROTEINS low density lipoproteins MAMMALS N-bromosuccinimide ORGANIC ACIDS ORGANIC COMPOUNDS Oxidation-Reduction OXIDIZERS PROTEINS radioiodination REDOX REACTIONS RUMINANTS Serum Albumin, Bovine SUCCINIC ACID Succinimides - pharmacology TRACER TECHNIQUES VERTEBRATES 550201 -- Biochemistry-- Tracer Techniques |
| title | Radioiodination of proteins and lipoproteins using N-bromosuccinimide as oxidizing agent |
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