Clinical Utility of Thyrotropin-Receptor Antibody Assays: Comparison of Radioreceptor and Bioassay Methods
Thyrotropin (thyroid-stimulating hormone or TSH)-receptor antibodies, important in the pathogenesis of Graves' disease, can be assayed by one of two methods: (1) bioassays that measure stimulation of thyroid cellular activity by patient immunoglobulins or (2) radioreceptor assays that measure i...
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| Veröffentlicht in: | Mayo Clinic proceedings 1988-07, Vol.63 (7), p.707-717 |
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| description | Thyrotropin (thyroid-stimulating hormone or TSH)-receptor antibodies, important in the pathogenesis of Graves' disease, can be assayed by one of two methods: (1) bioassays that measure stimulation of thyroid cellular activity by patient immunoglobulins or (2) radioreceptor assays that measure inhibition of binding of labeled TSH to TSH receptors by the same substances. In this study, we report our experience with bioassay of thyroid-stimulating immunoglobulins (TSI) based on measurement of generation of cyclic adenosine monophosphate in a clone of the Fisher rat thyroid cell line (FRTL-5) in 279 patients, and we compare, in 163 consecutive samples, the results obtained by a radioreceptor assay for thyrotropin-binding inhibiting immunoglobulins (TBII). Among the untreated, hyperthyroid patients with Graves' disease, TSI were present in 95% (38 of 40), and TBII were present in 85% (17 of 20). In patients with euthyroid Graves' disease, TSI were found in 57% (16 of 28), and TBII were present in 41% (7 of 17). Of 49 nongoitrous and euthyroid controls, only 4% had TSI and 3% had TBII. Extremely high TSI indices were found in all patients who had pretibial dermopathy (N = 10) or severe Graves' ophthalmopathy (N = 19) requiring orbital decompression. We conclude that both assays are highly sensitive and specific in diagnosing Graves' disease. The TSI bioassay was more sensitive (P |
| doi_str_mv | 10.1016/S0025-6196(12)65533-5 |
| format | Article |
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In this study, we report our experience with bioassay of thyroid-stimulating immunoglobulins (TSI) based on measurement of generation of cyclic adenosine monophosphate in a clone of the Fisher rat thyroid cell line (FRTL-5) in 279 patients, and we compare, in 163 consecutive samples, the results obtained by a radioreceptor assay for thyrotropin-binding inhibiting immunoglobulins (TBII). Among the untreated, hyperthyroid patients with Graves' disease, TSI were present in 95% (38 of 40), and TBII were present in 85% (17 of 20). In patients with euthyroid Graves' disease, TSI were found in 57% (16 of 28), and TBII were present in 41% (7 of 17). Of 49 nongoitrous and euthyroid controls, only 4% had TSI and 3% had TBII. Extremely high TSI indices were found in all patients who had pretibial dermopathy (N = 10) or severe Graves' ophthalmopathy (N = 19) requiring orbital decompression. We conclude that both assays are highly sensitive and specific in diagnosing Graves' disease. The TSI bioassay was more sensitive (P<0.001) than the TBII radioreceptor assay in detection of Graves' disease. In our experience, both assays have proved useful in the diagnosis of euthyroid Graves' disease with ophthalmopathy and atypical manifestations of hyperthyroid Graves' disease.</description><identifier>ISSN: 0025-6196</identifier><identifier>EISSN: 1942-5546</identifier><identifier>DOI: 10.1016/S0025-6196(12)65533-5</identifier><identifier>PMID: 2898572</identifier><identifier>CODEN: MACPAJ</identifier><language>eng</language><publisher>Rochester, MN: Elsevier Inc</publisher><subject>Antibodies - analysis ; Autoantibodies - analysis ; Biological and medical sciences ; Biological Assay - standards ; Cyclic AMP - analysis ; Endocrinology ; Evaluation Studies as Topic ; Eye Diseases - diagnosis ; Eye Diseases - etiology ; Eye Diseases - immunology ; Graves Disease - complications ; Graves Disease - diagnosis ; Graves Disease - immunology ; Humans ; Hyperparathyroidism, Secondary - diagnosis ; Hyperparathyroidism, Secondary - etiology ; Hyperparathyroidism, Secondary - immunology ; Immunoglobulin G - analysis ; Immunoglobulins, Thyroid-Stimulating ; Investigative techniques, diagnostic techniques (general aspects) ; Medical sciences ; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques ; Predictive Value of Tests ; Radioligand Assay - methods ; Radioligand Assay - standards ; Reagent Kits, Diagnostic ; Receptors, Thyrotropin - immunology ; Retrospective Studies ; Thyroid Gland - immunology ; Time Factors</subject><ispartof>Mayo Clinic proceedings, 1988-07, Vol.63 (7), p.707-717</ispartof><rights>1988 Mayo Foundation for Medical Education and Research</rights><rights>1989 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c389t-fe34acd5b273f84d304b8d3601a9f2fbf8ce581ae6361401e396949befa2bcf63</citedby><cites>FETCH-LOGICAL-c389t-fe34acd5b273f84d304b8d3601a9f2fbf8ce581ae6361401e396949befa2bcf63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,778,782,27907,27908</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7198101$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2898572$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>MORRIS, JOHN C.</creatorcontrib><creatorcontrib>HAY, IAN D.</creatorcontrib><creatorcontrib>NELSON, ROBERT E.</creatorcontrib><creatorcontrib>JIANG, NAI-SIANG</creatorcontrib><title>Clinical Utility of Thyrotropin-Receptor Antibody Assays: Comparison of Radioreceptor and Bioassay Methods</title><title>Mayo Clinic proceedings</title><addtitle>Mayo Clin Proc</addtitle><description>Thyrotropin (thyroid-stimulating hormone or TSH)-receptor antibodies, important in the pathogenesis of Graves' disease, can be assayed by one of two methods: (1) bioassays that measure stimulation of thyroid cellular activity by patient immunoglobulins or (2) radioreceptor assays that measure inhibition of binding of labeled TSH to TSH receptors by the same substances. In this study, we report our experience with bioassay of thyroid-stimulating immunoglobulins (TSI) based on measurement of generation of cyclic adenosine monophosphate in a clone of the Fisher rat thyroid cell line (FRTL-5) in 279 patients, and we compare, in 163 consecutive samples, the results obtained by a radioreceptor assay for thyrotropin-binding inhibiting immunoglobulins (TBII). Among the untreated, hyperthyroid patients with Graves' disease, TSI were present in 95% (38 of 40), and TBII were present in 85% (17 of 20). In patients with euthyroid Graves' disease, TSI were found in 57% (16 of 28), and TBII were present in 41% (7 of 17). Of 49 nongoitrous and euthyroid controls, only 4% had TSI and 3% had TBII. Extremely high TSI indices were found in all patients who had pretibial dermopathy (N = 10) or severe Graves' ophthalmopathy (N = 19) requiring orbital decompression. We conclude that both assays are highly sensitive and specific in diagnosing Graves' disease. The TSI bioassay was more sensitive (P<0.001) than the TBII radioreceptor assay in detection of Graves' disease. In our experience, both assays have proved useful in the diagnosis of euthyroid Graves' disease with ophthalmopathy and atypical manifestations of hyperthyroid Graves' disease.</description><subject>Antibodies - analysis</subject><subject>Autoantibodies - analysis</subject><subject>Biological and medical sciences</subject><subject>Biological Assay - standards</subject><subject>Cyclic AMP - analysis</subject><subject>Endocrinology</subject><subject>Evaluation Studies as Topic</subject><subject>Eye Diseases - diagnosis</subject><subject>Eye Diseases - etiology</subject><subject>Eye Diseases - immunology</subject><subject>Graves Disease - complications</subject><subject>Graves Disease - diagnosis</subject><subject>Graves Disease - immunology</subject><subject>Humans</subject><subject>Hyperparathyroidism, Secondary - diagnosis</subject><subject>Hyperparathyroidism, Secondary - etiology</subject><subject>Hyperparathyroidism, Secondary - immunology</subject><subject>Immunoglobulin G - analysis</subject><subject>Immunoglobulins, Thyroid-Stimulating</subject><subject>Investigative techniques, diagnostic techniques (general aspects)</subject><subject>Medical sciences</subject><subject>Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</subject><subject>Predictive Value of Tests</subject><subject>Radioligand Assay - methods</subject><subject>Radioligand Assay - standards</subject><subject>Reagent Kits, Diagnostic</subject><subject>Receptors, Thyrotropin - immunology</subject><subject>Retrospective Studies</subject><subject>Thyroid Gland - immunology</subject><subject>Time Factors</subject><issn>0025-6196</issn><issn>1942-5546</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1988</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1v1DAQhi0EKtvCT6iUA0JwCPg7NpdqWZUPqQiptGfLsceqq2wcbC9S_j3Z7rJXTnOY531n9CB0SfAHgon8-AtjKlpJtHxH6HspBGOteIZWRHPaCsHlc7Q6IS_ReSmPGONOa36GzqjSSnR0hR43Qxyjs0NzX-MQ69yk0Nw9zDnVnKY4trfgYKopN-uxxj75uVmXYufyqdmk7WRzLGncZ26tjyn_g-3om88x2T3a_ID6kHx5hV4EOxR4fZwX6P7L9d3mW3vz8-v3zfqmdUzp2gZg3DovetqxoLhnmPfKM4mJ1YGGPigHQhELkknCMQGmpea6h2Bp74JkF-jtoXfK6fcOSjXbWBwMgx0h7YrpFNVcYbWA4gC6nErJEMyU49bm2RBs9o7Nk2OzF2gINU-OjVhyl8cDu34L_pQ6Sl32b457WxazIdvRxXLCOqLV0r5gVwcMFhl_ImRTXITRgY-Lx2p8iv955C_nI5o7</recordid><startdate>19880701</startdate><enddate>19880701</enddate><creator>MORRIS, JOHN C.</creator><creator>HAY, IAN D.</creator><creator>NELSON, ROBERT E.</creator><creator>JIANG, NAI-SIANG</creator><general>Elsevier Inc</general><general>Mayo Medical Ventures</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19880701</creationdate><title>Clinical Utility of Thyrotropin-Receptor Antibody Assays: Comparison of Radioreceptor and Bioassay Methods</title><author>MORRIS, JOHN C. ; HAY, IAN D. ; NELSON, ROBERT E. ; JIANG, NAI-SIANG</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c389t-fe34acd5b273f84d304b8d3601a9f2fbf8ce581ae6361401e396949befa2bcf63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1988</creationdate><topic>Antibodies - analysis</topic><topic>Autoantibodies - analysis</topic><topic>Biological and medical sciences</topic><topic>Biological Assay - standards</topic><topic>Cyclic AMP - analysis</topic><topic>Endocrinology</topic><topic>Evaluation Studies as Topic</topic><topic>Eye Diseases - diagnosis</topic><topic>Eye Diseases - etiology</topic><topic>Eye Diseases - immunology</topic><topic>Graves Disease - complications</topic><topic>Graves Disease - diagnosis</topic><topic>Graves Disease - immunology</topic><topic>Humans</topic><topic>Hyperparathyroidism, Secondary - diagnosis</topic><topic>Hyperparathyroidism, Secondary - etiology</topic><topic>Hyperparathyroidism, Secondary - immunology</topic><topic>Immunoglobulin G - analysis</topic><topic>Immunoglobulins, Thyroid-Stimulating</topic><topic>Investigative techniques, diagnostic techniques (general aspects)</topic><topic>Medical sciences</topic><topic>Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</topic><topic>Predictive Value of Tests</topic><topic>Radioligand Assay - methods</topic><topic>Radioligand Assay - standards</topic><topic>Reagent Kits, Diagnostic</topic><topic>Receptors, Thyrotropin - immunology</topic><topic>Retrospective Studies</topic><topic>Thyroid Gland - immunology</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>MORRIS, JOHN C.</creatorcontrib><creatorcontrib>HAY, IAN D.</creatorcontrib><creatorcontrib>NELSON, ROBERT E.</creatorcontrib><creatorcontrib>JIANG, NAI-SIANG</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Mayo Clinic proceedings</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>MORRIS, JOHN C.</au><au>HAY, IAN D.</au><au>NELSON, ROBERT E.</au><au>JIANG, NAI-SIANG</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Clinical Utility of Thyrotropin-Receptor Antibody Assays: Comparison of Radioreceptor and Bioassay Methods</atitle><jtitle>Mayo Clinic proceedings</jtitle><addtitle>Mayo Clin Proc</addtitle><date>1988-07-01</date><risdate>1988</risdate><volume>63</volume><issue>7</issue><spage>707</spage><epage>717</epage><pages>707-717</pages><issn>0025-6196</issn><eissn>1942-5546</eissn><coden>MACPAJ</coden><abstract>Thyrotropin (thyroid-stimulating hormone or TSH)-receptor antibodies, important in the pathogenesis of Graves' disease, can be assayed by one of two methods: (1) bioassays that measure stimulation of thyroid cellular activity by patient immunoglobulins or (2) radioreceptor assays that measure inhibition of binding of labeled TSH to TSH receptors by the same substances. In this study, we report our experience with bioassay of thyroid-stimulating immunoglobulins (TSI) based on measurement of generation of cyclic adenosine monophosphate in a clone of the Fisher rat thyroid cell line (FRTL-5) in 279 patients, and we compare, in 163 consecutive samples, the results obtained by a radioreceptor assay for thyrotropin-binding inhibiting immunoglobulins (TBII). Among the untreated, hyperthyroid patients with Graves' disease, TSI were present in 95% (38 of 40), and TBII were present in 85% (17 of 20). In patients with euthyroid Graves' disease, TSI were found in 57% (16 of 28), and TBII were present in 41% (7 of 17). Of 49 nongoitrous and euthyroid controls, only 4% had TSI and 3% had TBII. Extremely high TSI indices were found in all patients who had pretibial dermopathy (N = 10) or severe Graves' ophthalmopathy (N = 19) requiring orbital decompression. We conclude that both assays are highly sensitive and specific in diagnosing Graves' disease. The TSI bioassay was more sensitive (P<0.001) than the TBII radioreceptor assay in detection of Graves' disease. In our experience, both assays have proved useful in the diagnosis of euthyroid Graves' disease with ophthalmopathy and atypical manifestations of hyperthyroid Graves' disease.</abstract><cop>Rochester, MN</cop><pub>Elsevier Inc</pub><pmid>2898572</pmid><doi>10.1016/S0025-6196(12)65533-5</doi><tpages>11</tpages></addata></record> |
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| ispartof | Mayo Clinic proceedings, 1988-07, Vol.63 (7), p.707-717 |
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| subjects | Antibodies - analysis Autoantibodies - analysis Biological and medical sciences Biological Assay - standards Cyclic AMP - analysis Endocrinology Evaluation Studies as Topic Eye Diseases - diagnosis Eye Diseases - etiology Eye Diseases - immunology Graves Disease - complications Graves Disease - diagnosis Graves Disease - immunology Humans Hyperparathyroidism, Secondary - diagnosis Hyperparathyroidism, Secondary - etiology Hyperparathyroidism, Secondary - immunology Immunoglobulin G - analysis Immunoglobulins, Thyroid-Stimulating Investigative techniques, diagnostic techniques (general aspects) Medical sciences Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques Predictive Value of Tests Radioligand Assay - methods Radioligand Assay - standards Reagent Kits, Diagnostic Receptors, Thyrotropin - immunology Retrospective Studies Thyroid Gland - immunology Time Factors |
| title | Clinical Utility of Thyrotropin-Receptor Antibody Assays: Comparison of Radioreceptor and Bioassay Methods |
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