Expression and activity of low Km, cGMP-inhibited cAMP phosphodiesterase in cardiac and skeletal muscle
The expression and activity of low Km, cGMP-inhibited cAMP phosphodiesterase (PDE3)4 were examined in rabbit and canine cardiac and skeletal muscle. In cardiac muscle, a cDNA probe whose sequence encompasses the catalytic domain of human myocardial PDE3 (PDE3A) hybridized predominantly with a 7.2-7....
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| Veröffentlicht in: | Biochemical and biophysical research communications 1996-08, Vol.225 (3), p.1058-1062 |
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| container_title | Biochemical and biophysical research communications |
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| creator | Movsesian, M A Komas, N Krall, J Manganiello, V C |
| description | The expression and activity of low Km, cGMP-inhibited cAMP phosphodiesterase (PDE3)4 were examined in rabbit and canine cardiac and skeletal muscle. In cardiac muscle, a cDNA probe whose sequence encompasses the catalytic domain of human myocardial PDE3 (PDE3A) hybridized predominantly with a 7.2-7.4 kb mRNA. No hybridization was observed in preparations from slow or fast twitch skeletal muscle. Likewise, PDE3 activity was present in cytosolic and microsomal fractions of cardiac muscle but was absent from cytosolic and microsomal fractions of slow twitch and fast twitch skeletal muscle. These results, which demonstrate the absence of PDE3 from slow and fast twitch mammalian skeletal muscle, further delineate the differences in beta-adrenergic receptor-mediated signal transduction pathways in cardiac and skeletal muscle. |
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These results, which demonstrate the absence of PDE3 from slow and fast twitch mammalian skeletal muscle, further delineate the differences in beta-adrenergic receptor-mediated signal transduction pathways in cardiac and skeletal muscle.</description><subject>3',5'-Cyclic-AMP Phosphodiesterases - genetics</subject><subject>3',5'-Cyclic-AMP Phosphodiesterases - metabolism</subject><subject>Animals</subject><subject>Cyclic Nucleotide Phosphodiesterases, Type 3</subject><subject>Cytosol - enzymology</subject><subject>Dogs</subject><subject>Gene Expression</subject><subject>Humans</subject><subject>In Vitro Techniques</subject><subject>Kinetics</subject><subject>Microsomes - enzymology</subject><subject>Muscle, Skeletal - enzymology</subject><subject>Myocardium - enzymology</subject><subject>Rabbits</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><issn>0006-291X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNotkDtPwzAYRT2ASin8BCRPTERy4lc8VlUpFa3o0IEtcuwv1OA8sBOg_54IMlzd5ejo6l6gOSFEJJlKX6_QdYzvhKQpE2qGZrnMiaR0jt7WP12AGF3bYN1YrE3vvlx_xm2FffuNn-sHbDb7Q-KakytdDxab5f6Au1Mbx1gHsYegI2DXYKODddr8ieIHeOi1x_UQjYcbdFlpH-F26gU6Pq6Pq6dk97LZrpa7pOOUJlpnRpCMWlFCyW1pQFRMGaEs40YpzQSXaUpYaaTlNCXCVlwyoFQCF2Q0LND9v7YL7ecwbitqFw14rxtoh1jIPFNEKTWCdxM4lDXYoguu1uFcTMfQX_HSXog</recordid><startdate>19960823</startdate><enddate>19960823</enddate><creator>Movsesian, M A</creator><creator>Komas, N</creator><creator>Krall, J</creator><creator>Manganiello, V C</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>19960823</creationdate><title>Expression and activity of low Km, cGMP-inhibited cAMP phosphodiesterase in cardiac and skeletal muscle</title><author>Movsesian, M A ; Komas, N ; Krall, J ; Manganiello, V C</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p533-aa2c6023d6beb5dbce6f49c69d45c99a46571104bc7d53106df574e337e560533</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>3',5'-Cyclic-AMP Phosphodiesterases - genetics</topic><topic>3',5'-Cyclic-AMP Phosphodiesterases - metabolism</topic><topic>Animals</topic><topic>Cyclic Nucleotide Phosphodiesterases, Type 3</topic><topic>Cytosol - enzymology</topic><topic>Dogs</topic><topic>Gene Expression</topic><topic>Humans</topic><topic>In Vitro Techniques</topic><topic>Kinetics</topic><topic>Microsomes - enzymology</topic><topic>Muscle, Skeletal - enzymology</topic><topic>Myocardium - enzymology</topic><topic>Rabbits</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Movsesian, M A</creatorcontrib><creatorcontrib>Komas, N</creatorcontrib><creatorcontrib>Krall, J</creatorcontrib><creatorcontrib>Manganiello, V C</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Movsesian, M A</au><au>Komas, N</au><au>Krall, J</au><au>Manganiello, V C</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression and activity of low Km, cGMP-inhibited cAMP phosphodiesterase in cardiac and skeletal muscle</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1996-08-23</date><risdate>1996</risdate><volume>225</volume><issue>3</issue><spage>1058</spage><epage>1062</epage><pages>1058-1062</pages><issn>0006-291X</issn><abstract>The expression and activity of low Km, cGMP-inhibited cAMP phosphodiesterase (PDE3)4 were examined in rabbit and canine cardiac and skeletal muscle. 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| subjects | 3',5'-Cyclic-AMP Phosphodiesterases - genetics 3',5'-Cyclic-AMP Phosphodiesterases - metabolism Animals Cyclic Nucleotide Phosphodiesterases, Type 3 Cytosol - enzymology Dogs Gene Expression Humans In Vitro Techniques Kinetics Microsomes - enzymology Muscle, Skeletal - enzymology Myocardium - enzymology Rabbits RNA, Messenger - genetics RNA, Messenger - metabolism |
| title | Expression and activity of low Km, cGMP-inhibited cAMP phosphodiesterase in cardiac and skeletal muscle |
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