Flow cytometric analysis of Epstein-Barr virus receptor among the different B-cell subpopulations using simultaneous two-color immunofluorescence
The distribution of Epstein-Barr virus receptor (EBVR) among the different B-cell subpopulations was analyzed by flow cytometry, using simultaneous two-color immunofluorescence of EBVR and cell-surface markers. The expression of EBVR was established by the binding of fluorescein isothiocyanate (FITC...
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| Veröffentlicht in: | Virology (New York, N.Y.) N.Y.), 1988-07, Vol.165 (1), p.278-281 |
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| container_title | Virology (New York, N.Y.) |
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| creator | Harabuchi, Yasuaki Koizumi, Shigeki Osato, Toyoro Yamanaka, Noboru Kataura, Akikatsu |
| description | The distribution of Epstein-Barr virus receptor (EBVR) among the different B-cell subpopulations was analyzed by flow cytometry, using simultaneous two-color immunofluorescence of EBVR and cell-surface markers. The expression of EBVR was established by the binding of fluorescein isothiocyanate (FITC)-labeled EBV to the cells, while surface markers were stained by phycoerythrin (PE)-indirect immunofluorescence, using monoclonal antibodies. All cells of both the resting B-cell subpopulation defined by L30 and the B-cell subpopulations expressing surface IgM, IgD, IgA, and IgG were EBVR-positive. In contrast, EBVR was absent from about 10% cells of the activated B-cell subpopulation recognized by OKT9, as well as from about 10% cells of the highly differentiated B-cell subpopulation which reacted with OKT10. These results suggest that the expression of EBVR on the B-cell lineage varies with the maturation stage and with the state of activation. This postulation was further supported by analyses based on
in vitro B-cell activation. |
| doi_str_mv | 10.1016/0042-6822(88)90683-6 |
| format | Article |
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in vitro B-cell activation.</description><subject>B-Lymphocytes - analysis</subject><subject>B-Lymphocytes - classification</subject><subject>Biological and medical sciences</subject><subject>Cell Separation - methods</subject><subject>Epstein-Barr virus</subject><subject>Flow Cytometry</subject><subject>Fluorescent Antibody Technique</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Herpesvirus 4, Human - metabolism</subject><subject>immunofluorescence</subject><subject>lymphocytes B</subject><subject>Microbiology</subject><subject>Receptors, Virus - analysis</subject><subject>Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains</subject><subject>Virology</subject><issn>0042-6822</issn><issn>1096-0341</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1988</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1u1DAURi0EKtPCG4DkBUKwCNhO4jgbJFq1gFSJDawt5-YajJw4-KfVPAZvTNIZzRJWtnWPP3_yIeQFZ-844_I9Y42opBLijVJveyZVXclHZMdZLytWN_wx2Z2Qp-Q8pV9sPXcdOyNnQtWql-2O_Lnx4Z7CPocJc3RAzWz8PrlEg6XXS8ro5urSxEjvXCyJRgRccojUTGH-QfNPpKOzFiPOmV5WgN7TVIYlLMWb7MKcaEluJZObis9mxrCm5PtQQfBrjJumMgfrS4iYAGfAZ-SJNT7h8-N6Qb7fXH-7-lzdfv305erjbQV1LXJl7GAaAGiGEdFCN7SyaaEeBfB1x4e2A2m5MWjVYDsl7dC1BoVRtjetULK-IK8PuUsMvwumrCeXtv6HjrpTQvUdZ_8FedNLJtsNbA4gxJBSRKuX6CYT95ozvSnTmw-9-dBK6Qdleivy8phfhgnH06Wjo3X-6jg3CYy30czg0gnrWF8zsb3-4YDh-ml3DqNO4LYPHd0qLesxuH_3-Avbhbe6</recordid><startdate>198807</startdate><enddate>198807</enddate><creator>Harabuchi, Yasuaki</creator><creator>Koizumi, Shigeki</creator><creator>Osato, Toyoro</creator><creator>Yamanaka, Noboru</creator><creator>Kataura, Akikatsu</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>M7Z</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>198807</creationdate><title>Flow cytometric analysis of Epstein-Barr virus receptor among the different B-cell subpopulations using simultaneous two-color immunofluorescence</title><author>Harabuchi, Yasuaki ; Koizumi, Shigeki ; Osato, Toyoro ; Yamanaka, Noboru ; Kataura, Akikatsu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c332t-afba4ccc4bdeefc7b5645c3d2c15641b57c6f1aaef8bf786fb75ae2a8f9a52863</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1988</creationdate><topic>B-Lymphocytes - analysis</topic><topic>B-Lymphocytes - classification</topic><topic>Biological and medical sciences</topic><topic>Cell Separation - methods</topic><topic>Epstein-Barr virus</topic><topic>Flow Cytometry</topic><topic>Fluorescent Antibody Technique</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Herpesvirus 4, Human - metabolism</topic><topic>immunofluorescence</topic><topic>lymphocytes B</topic><topic>Microbiology</topic><topic>Receptors, Virus - analysis</topic><topic>Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Harabuchi, Yasuaki</creatorcontrib><creatorcontrib>Koizumi, Shigeki</creatorcontrib><creatorcontrib>Osato, Toyoro</creatorcontrib><creatorcontrib>Yamanaka, Noboru</creatorcontrib><creatorcontrib>Kataura, Akikatsu</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biochemistry Abstracts 1</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Virology (New York, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Harabuchi, Yasuaki</au><au>Koizumi, Shigeki</au><au>Osato, Toyoro</au><au>Yamanaka, Noboru</au><au>Kataura, Akikatsu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Flow cytometric analysis of Epstein-Barr virus receptor among the different B-cell subpopulations using simultaneous two-color immunofluorescence</atitle><jtitle>Virology (New York, N.Y.)</jtitle><addtitle>Virology</addtitle><date>1988-07</date><risdate>1988</risdate><volume>165</volume><issue>1</issue><spage>278</spage><epage>281</epage><pages>278-281</pages><issn>0042-6822</issn><eissn>1096-0341</eissn><coden>VIRLAX</coden><abstract>The distribution of Epstein-Barr virus receptor (EBVR) among the different B-cell subpopulations was analyzed by flow cytometry, using simultaneous two-color immunofluorescence of EBVR and cell-surface markers. The expression of EBVR was established by the binding of fluorescein isothiocyanate (FITC)-labeled EBV to the cells, while surface markers were stained by phycoerythrin (PE)-indirect immunofluorescence, using monoclonal antibodies. All cells of both the resting B-cell subpopulation defined by L30 and the B-cell subpopulations expressing surface IgM, IgD, IgA, and IgG were EBVR-positive. In contrast, EBVR was absent from about 10% cells of the activated B-cell subpopulation recognized by OKT9, as well as from about 10% cells of the highly differentiated B-cell subpopulation which reacted with OKT10. These results suggest that the expression of EBVR on the B-cell lineage varies with the maturation stage and with the state of activation. This postulation was further supported by analyses based on
in vitro B-cell activation.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>2838965</pmid><doi>10.1016/0042-6822(88)90683-6</doi><tpages>4</tpages></addata></record> |
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| ispartof | Virology (New York, N.Y.), 1988-07, Vol.165 (1), p.278-281 |
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| subjects | B-Lymphocytes - analysis B-Lymphocytes - classification Biological and medical sciences Cell Separation - methods Epstein-Barr virus Flow Cytometry Fluorescent Antibody Technique Fundamental and applied biological sciences. Psychology Herpesvirus 4, Human - metabolism immunofluorescence lymphocytes B Microbiology Receptors, Virus - analysis Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains Virology |
| title | Flow cytometric analysis of Epstein-Barr virus receptor among the different B-cell subpopulations using simultaneous two-color immunofluorescence |
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