Differentiation potential of 14-day fetal mouse thymocytes in organ culture. Analysis of CD4/CD8-defined single-positive and double- negative cells
A two-color flow cytometric analysis was carried out on 14-day fetal mouse thymus lobes maintained for various lengths of time in organ culture. Kinetic analysis showed that the proportions of CD4- and CD8-defined subpopulations changed with time. In particular, there appeared to be accumulation of...
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| Veröffentlicht in: | The Journal of immunology (1950) 1988-07, Vol.141 (2), p.355-362 |
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| container_title | The Journal of immunology (1950) |
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| creator | Ceredig, R |
| description | A two-color flow cytometric analysis was carried out on 14-day fetal mouse thymus lobes maintained for various lengths of time in organ culture. Kinetic analysis showed that the proportions of CD4- and CD8-defined subpopulations changed with time. In particular, there appeared to be accumulation of J11d-, CD4/CD8 double-negative and J11d-, Ly-6A+ single-positive cells, a phenotype associated with "mature" cells. Gated two-color analysis of CD4/CD8 single-positive cells showed that they contained distinct subpopulations as defined by mAb to CD3, TCR (F23.1), Ly-6A, Ly-6C, LFA-1, and J11d. Thus, the precursor cells found in the 14-day fetal mouse thymus were capable of generating all the subpopulations of CD4/CD8-defined single-positive and double-negative cells seen in the normal adult mouse thymus. |
| doi_str_mv | 10.4049/jimmunol.141.2.355 |
| format | Article |
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Thus, the precursor cells found in the 14-day fetal mouse thymus were capable of generating all the subpopulations of CD4/CD8-defined single-positive and double-negative cells seen in the normal adult mouse thymus.</description><identifier>ISSN: 0022-1767</identifier><identifier>EISSN: 1550-6606</identifier><identifier>DOI: 10.4049/jimmunol.141.2.355</identifier><identifier>PMID: 3260250</identifier><identifier>CODEN: JOIMA3</identifier><language>eng</language><publisher>Bethesda, MD: Am Assoc Immnol</publisher><subject>Analysis of the immune response. Humoral and cellular immunity ; Animals ; Antibodies, Monoclonal ; Antigens, Differentiation, T-Lymphocyte - analysis ; Biological and medical sciences ; Cell Differentiation ; Female ; Fetus - immunology ; Flow Cytometry ; Fundamental and applied biological sciences. Psychology ; Fundamental immunology ; Immunobiology ; Lymphocyte Activation ; Mice ; Mice, Inbred C57BL ; Organ Culture Techniques ; Organs and cells involved in the immune response ; Phenotype ; Scattering, Radiation ; T-Lymphocytes - classification ; T-Lymphocytes - cytology ; T-Lymphocytes - immunology</subject><ispartof>The Journal of immunology (1950), 1988-07, Vol.141 (2), p.355-362</ispartof><rights>1989 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c433t-fbe3cfbd216187583357708ab802dfc820ce759a7949926338824c1a0546e5363</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7322941$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3260250$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ceredig, R</creatorcontrib><title>Differentiation potential of 14-day fetal mouse thymocytes in organ culture. Analysis of CD4/CD8-defined single-positive and double- negative cells</title><title>The Journal of immunology (1950)</title><addtitle>J Immunol</addtitle><description>A two-color flow cytometric analysis was carried out on 14-day fetal mouse thymus lobes maintained for various lengths of time in organ culture. Kinetic analysis showed that the proportions of CD4- and CD8-defined subpopulations changed with time. In particular, there appeared to be accumulation of J11d-, CD4/CD8 double-negative and J11d-, Ly-6A+ single-positive cells, a phenotype associated with "mature" cells. Gated two-color analysis of CD4/CD8 single-positive cells showed that they contained distinct subpopulations as defined by mAb to CD3, TCR (F23.1), Ly-6A, Ly-6C, LFA-1, and J11d. Thus, the precursor cells found in the 14-day fetal mouse thymus were capable of generating all the subpopulations of CD4/CD8-defined single-positive and double-negative cells seen in the normal adult mouse thymus.</description><subject>Analysis of the immune response. Humoral and cellular immunity</subject><subject>Animals</subject><subject>Antibodies, Monoclonal</subject><subject>Antigens, Differentiation, T-Lymphocyte - analysis</subject><subject>Biological and medical sciences</subject><subject>Cell Differentiation</subject><subject>Female</subject><subject>Fetus - immunology</subject><subject>Flow Cytometry</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>Immunobiology</subject><subject>Lymphocyte Activation</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Organ Culture Techniques</subject><subject>Organs and cells involved in the immune response</subject><subject>Phenotype</subject><subject>Scattering, Radiation</subject><subject>T-Lymphocytes - classification</subject><subject>T-Lymphocytes - cytology</subject><subject>T-Lymphocytes - immunology</subject><issn>0022-1767</issn><issn>1550-6606</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1988</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1u1DAUhS0EKtPCCyAheYHYZer_JMtqBgpSJTawthznesaVYw-xwyjP0Rcm0w7tkpV97z3n-FofQh8oWQsi2ut7PwxTTGFNBV2zNZfyFVpRKUmlFFGv0YoQxipaq_otusz5nhCiCBMX6IKz5SLJCj1svXMwQizeFJ8iPqTyWAScHKai6s2MHZSlHtKUAZf9PCQ7F8jYR5zGnYnYTqFMI6zxTTRhzj6fvJutuN5sm6oH5yP0OPu4C1AdUvbF_wFsYo_7NHVLD0fYmcemhRDyO_TGmZDh_fm8Qr--fvm5-Vbd_bj9vrm5q6zgvFSuA25d1zOqaFPLhnNZ16QxXUNY72zDiIVatqZuRdsyxXnTMGGpIVIokFzxK_T5Kfcwpt8T5KIHn08bmAjLX3XdsFPu_4VUEtUoJhYhexLaMeU8gtOH0Q9mnDUl-oRM_0OmF2Sa6QXZYvp4Tp-6Afpny5nRMv90nptsTXCjidbnZ1nNGWsFfVly73f7ox9B58GEsIRSfTweX977CzJqrpQ</recordid><startdate>19880715</startdate><enddate>19880715</enddate><creator>Ceredig, R</creator><general>Am Assoc Immnol</general><general>American Association of Immunologists</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19880715</creationdate><title>Differentiation potential of 14-day fetal mouse thymocytes in organ culture. Analysis of CD4/CD8-defined single-positive and double- negative cells</title><author>Ceredig, R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c433t-fbe3cfbd216187583357708ab802dfc820ce759a7949926338824c1a0546e5363</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1988</creationdate><topic>Analysis of the immune response. Humoral and cellular immunity</topic><topic>Animals</topic><topic>Antibodies, Monoclonal</topic><topic>Antigens, Differentiation, T-Lymphocyte - analysis</topic><topic>Biological and medical sciences</topic><topic>Cell Differentiation</topic><topic>Female</topic><topic>Fetus - immunology</topic><topic>Flow Cytometry</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fundamental immunology</topic><topic>Immunobiology</topic><topic>Lymphocyte Activation</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Organ Culture Techniques</topic><topic>Organs and cells involved in the immune response</topic><topic>Phenotype</topic><topic>Scattering, Radiation</topic><topic>T-Lymphocytes - classification</topic><topic>T-Lymphocytes - cytology</topic><topic>T-Lymphocytes - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ceredig, R</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of immunology (1950)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ceredig, R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Differentiation potential of 14-day fetal mouse thymocytes in organ culture. Analysis of CD4/CD8-defined single-positive and double- negative cells</atitle><jtitle>The Journal of immunology (1950)</jtitle><addtitle>J Immunol</addtitle><date>1988-07-15</date><risdate>1988</risdate><volume>141</volume><issue>2</issue><spage>355</spage><epage>362</epage><pages>355-362</pages><issn>0022-1767</issn><eissn>1550-6606</eissn><coden>JOIMA3</coden><abstract>A two-color flow cytometric analysis was carried out on 14-day fetal mouse thymus lobes maintained for various lengths of time in organ culture. Kinetic analysis showed that the proportions of CD4- and CD8-defined subpopulations changed with time. In particular, there appeared to be accumulation of J11d-, CD4/CD8 double-negative and J11d-, Ly-6A+ single-positive cells, a phenotype associated with "mature" cells. Gated two-color analysis of CD4/CD8 single-positive cells showed that they contained distinct subpopulations as defined by mAb to CD3, TCR (F23.1), Ly-6A, Ly-6C, LFA-1, and J11d. Thus, the precursor cells found in the 14-day fetal mouse thymus were capable of generating all the subpopulations of CD4/CD8-defined single-positive and double-negative cells seen in the normal adult mouse thymus.</abstract><cop>Bethesda, MD</cop><pub>Am Assoc Immnol</pub><pmid>3260250</pmid><doi>10.4049/jimmunol.141.2.355</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | The Journal of immunology (1950), 1988-07, Vol.141 (2), p.355-362 |
| issn | 0022-1767 1550-6606 |
| language | eng |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Analysis of the immune response. Humoral and cellular immunity Animals Antibodies, Monoclonal Antigens, Differentiation, T-Lymphocyte - analysis Biological and medical sciences Cell Differentiation Female Fetus - immunology Flow Cytometry Fundamental and applied biological sciences. Psychology Fundamental immunology Immunobiology Lymphocyte Activation Mice Mice, Inbred C57BL Organ Culture Techniques Organs and cells involved in the immune response Phenotype Scattering, Radiation T-Lymphocytes - classification T-Lymphocytes - cytology T-Lymphocytes - immunology |
| title | Differentiation potential of 14-day fetal mouse thymocytes in organ culture. Analysis of CD4/CD8-defined single-positive and double- negative cells |
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