Shiga-like toxin purges human lymphoma from bone marrow of severe combined immunodeficient mice
Shiga-like toxin-1 (SLT-1) is a bacterial toxin that kills cells by inhibiting protein synthesis. SLT-1 is composed of one cytotoxic A-subunit and five B-subunits that bind to CD77, a cell-surface glycolipid. In the human hematopoietic system, CD77 expression is restricted to a subset of activated B...
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| Veröffentlicht in: | Blood 1996-09, Vol.88 (5), p.1561-1567 |
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| container_issue | 5 |
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| creator | LACASSE, E. C SALEH, M. T PATTERSON, B MINDEN, M. D GARIEPY, J |
| description | Shiga-like toxin-1 (SLT-1) is a bacterial toxin that kills cells by inhibiting protein synthesis. SLT-1 is composed of one cytotoxic A-subunit and five B-subunits that bind to CD77, a cell-surface glycolipid. In the human hematopoietic system, CD77 expression is restricted to a subset of activated B cells and derived cancers. Here we report that SLT-1 treatment of murine bone marrow ex vivo effectively cures severe combined immunodeficient mice of a human B-cell lymphoma xenograft while sparing normal hematopoietic precursor cells. Flow cytometry results using fluorescein isothiocyanate-labeled SLT-1 B-subunit show the high prevalence of expression of SLT-1 receptors (CD77) in human non-Hodgkin's lymphomas, especially follicular lymphomas. These results suggest the use of SLT-1 for the purging of human bone marrow before autologous bone marrow transplant in the case of CD77+ B-cell lymphomas as just one of many possible uses. |
| doi_str_mv | 10.1182/blood.V88.5.1561.1561 |
| format | Article |
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These results suggest the use of SLT-1 for the purging of human bone marrow before autologous bone marrow transplant in the case of CD77+ B-cell lymphomas as just one of many possible uses.</description><identifier>ISSN: 0006-4971</identifier><identifier>EISSN: 1528-0020</identifier><identifier>DOI: 10.1182/blood.V88.5.1561.1561</identifier><identifier>PMID: 8781410</identifier><language>eng</language><publisher>Washington, DC: The Americain Society of Hematology</publisher><subject>Animals ; Antineoplastic agents ; Antineoplastic Agents - metabolism ; Antineoplastic Agents - therapeutic use ; Bacterial Toxins - metabolism ; Bacterial Toxins - therapeutic use ; Biological and medical sciences ; Bone Marrow - drug effects ; Bone Marrow - pathology ; Bone Marrow Purging ; Bone Marrow Transplantation ; Burkitt Lymphoma - drug therapy ; Burkitt Lymphoma - pathology ; Female ; General aspects ; Humans ; Medical sciences ; Mice ; Mice, Inbred BALB C ; Mice, SCID ; Neoplasm Transplantation ; Pharmacology. Drug treatments ; Radiation Chimera ; Shiga Toxin 1 ; Specific Pathogen-Free Organisms ; Transplantation, Heterologous ; Trihexosylceramides - metabolism ; Tumor Stem Cell Assay</subject><ispartof>Blood, 1996-09, Vol.88 (5), p.1561-1567</ispartof><rights>1996 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c312t-a5bff3a2b635eb0ce24b830afba15e2297e6ce16f845a08129ec00e3ba9f63283</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3203698$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8781410$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>LACASSE, E. C</creatorcontrib><creatorcontrib>SALEH, M. T</creatorcontrib><creatorcontrib>PATTERSON, B</creatorcontrib><creatorcontrib>MINDEN, M. D</creatorcontrib><creatorcontrib>GARIEPY, J</creatorcontrib><title>Shiga-like toxin purges human lymphoma from bone marrow of severe combined immunodeficient mice</title><title>Blood</title><addtitle>Blood</addtitle><description>Shiga-like toxin-1 (SLT-1) is a bacterial toxin that kills cells by inhibiting protein synthesis. SLT-1 is composed of one cytotoxic A-subunit and five B-subunits that bind to CD77, a cell-surface glycolipid. In the human hematopoietic system, CD77 expression is restricted to a subset of activated B cells and derived cancers. Here we report that SLT-1 treatment of murine bone marrow ex vivo effectively cures severe combined immunodeficient mice of a human B-cell lymphoma xenograft while sparing normal hematopoietic precursor cells. Flow cytometry results using fluorescein isothiocyanate-labeled SLT-1 B-subunit show the high prevalence of expression of SLT-1 receptors (CD77) in human non-Hodgkin's lymphomas, especially follicular lymphomas. These results suggest the use of SLT-1 for the purging of human bone marrow before autologous bone marrow transplant in the case of CD77+ B-cell lymphomas as just one of many possible uses.</description><subject>Animals</subject><subject>Antineoplastic agents</subject><subject>Antineoplastic Agents - metabolism</subject><subject>Antineoplastic Agents - therapeutic use</subject><subject>Bacterial Toxins - metabolism</subject><subject>Bacterial Toxins - therapeutic use</subject><subject>Biological and medical sciences</subject><subject>Bone Marrow - drug effects</subject><subject>Bone Marrow - pathology</subject><subject>Bone Marrow Purging</subject><subject>Bone Marrow Transplantation</subject><subject>Burkitt Lymphoma - drug therapy</subject><subject>Burkitt Lymphoma - pathology</subject><subject>Female</subject><subject>General aspects</subject><subject>Humans</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Mice, SCID</subject><subject>Neoplasm Transplantation</subject><subject>Pharmacology. Drug treatments</subject><subject>Radiation Chimera</subject><subject>Shiga Toxin 1</subject><subject>Specific Pathogen-Free Organisms</subject><subject>Transplantation, Heterologous</subject><subject>Trihexosylceramides - metabolism</subject><subject>Tumor Stem Cell Assay</subject><issn>0006-4971</issn><issn>1528-0020</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kE9vGyEQxVGVKHHTfoRIHKLc1h1gweyxsvKnkqUe2vSKAA8xzbK44G2Tb9-1Y_ny5vDem9H8CLlmMGdM8y-uz3k9_6X1XM6ZVOwgH8iMSa4bAA5nZAYAqmm7BbskH2v9DcBaweUFudALzVoGM2J-bOKzbfr4gnSXX-NAt2N5xko3Y7ID7d_SdpOTpaHkRF0ekCZbSv5Hc6AV_2JB6nNyccA1jSmNQ15jiD7isKMpevxEzoPtK34-zivydH_3c_nYrL4_fFt-XTVeML5rrHQhCMudEhIdeOSt0wJscJZJ5LxboPLIVNCttKAZ79ADoHC2C0pwLa7I7fvebcl_Rqw7k2L12Pd2wDxWs9BcS6VgCsr3oC-51oLBbEucfnozDMwerDmANRNYI82e6UGm3vXxwOgSrk-tI8nJvzn6tnrbh2IHH-spJjgI1WnxHxYPhBw</recordid><startdate>19960901</startdate><enddate>19960901</enddate><creator>LACASSE, E. 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D ; GARIEPY, J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c312t-a5bff3a2b635eb0ce24b830afba15e2297e6ce16f845a08129ec00e3ba9f63283</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Animals</topic><topic>Antineoplastic agents</topic><topic>Antineoplastic Agents - metabolism</topic><topic>Antineoplastic Agents - therapeutic use</topic><topic>Bacterial Toxins - metabolism</topic><topic>Bacterial Toxins - therapeutic use</topic><topic>Biological and medical sciences</topic><topic>Bone Marrow - drug effects</topic><topic>Bone Marrow - pathology</topic><topic>Bone Marrow Purging</topic><topic>Bone Marrow Transplantation</topic><topic>Burkitt Lymphoma - drug therapy</topic><topic>Burkitt Lymphoma - pathology</topic><topic>Female</topic><topic>General aspects</topic><topic>Humans</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Mice, SCID</topic><topic>Neoplasm Transplantation</topic><topic>Pharmacology. Drug treatments</topic><topic>Radiation Chimera</topic><topic>Shiga Toxin 1</topic><topic>Specific Pathogen-Free Organisms</topic><topic>Transplantation, Heterologous</topic><topic>Trihexosylceramides - metabolism</topic><topic>Tumor Stem Cell Assay</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>LACASSE, E. C</creatorcontrib><creatorcontrib>SALEH, M. T</creatorcontrib><creatorcontrib>PATTERSON, B</creatorcontrib><creatorcontrib>MINDEN, M. 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D</au><au>GARIEPY, J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Shiga-like toxin purges human lymphoma from bone marrow of severe combined immunodeficient mice</atitle><jtitle>Blood</jtitle><addtitle>Blood</addtitle><date>1996-09-01</date><risdate>1996</risdate><volume>88</volume><issue>5</issue><spage>1561</spage><epage>1567</epage><pages>1561-1567</pages><issn>0006-4971</issn><eissn>1528-0020</eissn><abstract>Shiga-like toxin-1 (SLT-1) is a bacterial toxin that kills cells by inhibiting protein synthesis. SLT-1 is composed of one cytotoxic A-subunit and five B-subunits that bind to CD77, a cell-surface glycolipid. In the human hematopoietic system, CD77 expression is restricted to a subset of activated B cells and derived cancers. Here we report that SLT-1 treatment of murine bone marrow ex vivo effectively cures severe combined immunodeficient mice of a human B-cell lymphoma xenograft while sparing normal hematopoietic precursor cells. Flow cytometry results using fluorescein isothiocyanate-labeled SLT-1 B-subunit show the high prevalence of expression of SLT-1 receptors (CD77) in human non-Hodgkin's lymphomas, especially follicular lymphomas. These results suggest the use of SLT-1 for the purging of human bone marrow before autologous bone marrow transplant in the case of CD77+ B-cell lymphomas as just one of many possible uses.</abstract><cop>Washington, DC</cop><pub>The Americain Society of Hematology</pub><pmid>8781410</pmid><doi>10.1182/blood.V88.5.1561.1561</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Blood, 1996-09, Vol.88 (5), p.1561-1567 |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Animals Antineoplastic agents Antineoplastic Agents - metabolism Antineoplastic Agents - therapeutic use Bacterial Toxins - metabolism Bacterial Toxins - therapeutic use Biological and medical sciences Bone Marrow - drug effects Bone Marrow - pathology Bone Marrow Purging Bone Marrow Transplantation Burkitt Lymphoma - drug therapy Burkitt Lymphoma - pathology Female General aspects Humans Medical sciences Mice Mice, Inbred BALB C Mice, SCID Neoplasm Transplantation Pharmacology. Drug treatments Radiation Chimera Shiga Toxin 1 Specific Pathogen-Free Organisms Transplantation, Heterologous Trihexosylceramides - metabolism Tumor Stem Cell Assay |
| title | Shiga-like toxin purges human lymphoma from bone marrow of severe combined immunodeficient mice |
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