Characterisation of secondary metabolites associated with neutrophil apoptosis

We studied changes in secondary metabolites in human neutrophils undergoing constitutive or tumour necrosis factor (TNFα) stimulated apoptosis by a combination of high-performance liquid chromatography (HPLC) and NMR spectroscopy. Our results show that in contrast to freshly isolated neutrophils, ne...

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Veröffentlicht in:	FEBS letters 1996-09, Vol.392 (3), p.295-298
Hauptverfasser:	Nunn, Alistair V.W., Barnard, Maria L., Bhakoo, Kishore, Murray, Joanna, Chilvers, Edwin J., Bell, Jimmy D.
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenosine Diphosphate - analysis Adenosine Diphosphate - metabolism Adenosine Triphosphate - analysis Adenosine Triphosphate - metabolism Androstadienes - pharmacology Apoptosis Apoptosis - drug effects Apoptosis - physiology Cells, Cultured Chromatography, High Pressure Liquid Enzyme Inhibitors - pharmacology high-performance liquid chromatography HPLC Humans Lactate Magnetic Resonance Spectroscopy Neutrophils - drug effects Neutrophils - metabolism NMR P13K PBS PCA PCho perchloric acid phosphate-buffered saline Phosphocholine Phospholipase C phospholipase D Phosphorylcholine - metabolism phospinosotide-3-kinase PKC PLC PLD protein kinase C TNFα Tumor Necrosis Factor-alpha - pharmacology tumour necrosis factor Tyrosine - metabolism Wortmannin
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