A novel two-site binding equation presented in terms of the total ligand concentration
For the most frequently used two-site model, an exact binding equation is presented in terms of the total ligand concentration. This equation has been extended to analyze the spectroscopic titration experiment where the dilution of protein solution cannot be neglected, the displacement study, and th...
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| Veröffentlicht in: | FEBS letters 1996-09, Vol.392 (3), p.245-249 |
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| container_title | FEBS letters |
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| creator | Wang, Zhi-Xin Jiang, Ruo-Fan |
| description | For the most frequently used two-site model, an exact binding equation is presented in terms of the total ligand concentration. This equation has been extended to analyze the spectroscopic titration experiment where the dilution of protein solution cannot be neglected, the displacement study, and the effect of non-specific binding. Thus, with a non-linear regression program, all unknown binding parameters can be determined correctly by fitting these equations to the experimental data without any data transformation. As an example of the use of the new equations, the experimental data for receptor-insulin binding were taken from literature and reanalyzed by using a non-linear regression data analysis program. |
| doi_str_mv | 10.1016/0014-5793(96)00818-6 |
| format | Article |
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As an example of the use of the new equations, the experimental data for receptor-insulin binding were taken from literature and reanalyzed by using a non-linear regression data analysis program.</description><subject>Binding equation</subject><subject>Binding Sites</subject><subject>Chemistry Techniques, Analytical - methods</subject><subject>Displacement curve</subject><subject>Dissociation constant</subject><subject>Insulin - chemistry</subject><subject>Insulin - metabolism</subject><subject>Ligand-protein interaction</subject><subject>Ligands</subject><subject>Models, Chemical</subject><subject>Models, Molecular</subject><subject>Non-linear regression</subject><subject>Physical Phenomena</subject><subject>Physics</subject><subject>Proteins - chemistry</subject><subject>Proteins - metabolism</subject><subject>Regression Analysis</subject><subject>Spectrum Analysis - methods</subject><subject>Titrimetry</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkE1PHCEYx0nTRlfbb2ATTqY9TAsDw8vFxBrXNjHpRXslLDyjNLOwAqvx25dxNx5ND4Q8z_8F8kPohJJvlFDxnRDKu0Fq9kWLr4QoqjrxDi2okqxjXKj3aPFqOURHpfwlbVZUH6ADJSVXA1-gP-c4pkeYcH1KXQkV8CpEH-IdhoetrSFFvMlQIFbwOERcIa8LTiOu94BrqnbCU7iz0WOXomu2_BL6iD6MdirwaX8fo9vl5c3Fz-7699Wvi_Przg2sFx3rudZ01Y6m0ktYaeu1HbgdtFBydBzYSJl3ltBeWqn42Fs9stE5y4ilnB2j013vJqeHLZRq1qE4mCYbIW2LkapXTKihGfnO6HIqJcNoNjmsbX42lJgZp5lZmZmV0fPQcBrRYp_3_dvVGvxraM-v6cud_hQmeP6vTrO8_NHPwrzX4mU7P3S2K4JG6zFANsUFaER9yOCq8Sm8_dN_zweXtA</recordid><startdate>19960902</startdate><enddate>19960902</enddate><creator>Wang, Zhi-Xin</creator><creator>Jiang, Ruo-Fan</creator><general>Elsevier B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19960902</creationdate><title>A novel two-site binding equation presented in terms of the total ligand concentration</title><author>Wang, Zhi-Xin ; Jiang, Ruo-Fan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5326-324991b991917d7eb9ad9a54a59687fc4e3f13dca0127a784f2a9f3fcca30a143</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Binding equation</topic><topic>Binding Sites</topic><topic>Chemistry Techniques, Analytical - methods</topic><topic>Displacement curve</topic><topic>Dissociation constant</topic><topic>Insulin - chemistry</topic><topic>Insulin - metabolism</topic><topic>Ligand-protein interaction</topic><topic>Ligands</topic><topic>Models, Chemical</topic><topic>Models, Molecular</topic><topic>Non-linear regression</topic><topic>Physical Phenomena</topic><topic>Physics</topic><topic>Proteins - chemistry</topic><topic>Proteins - metabolism</topic><topic>Regression Analysis</topic><topic>Spectrum Analysis - methods</topic><topic>Titrimetry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Zhi-Xin</creatorcontrib><creatorcontrib>Jiang, Ruo-Fan</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Zhi-Xin</au><au>Jiang, Ruo-Fan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A novel two-site binding equation presented in terms of the total ligand concentration</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>1996-09-02</date><risdate>1996</risdate><volume>392</volume><issue>3</issue><spage>245</spage><epage>249</epage><pages>245-249</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><abstract>For the most frequently used two-site model, an exact binding equation is presented in terms of the total ligand concentration. This equation has been extended to analyze the spectroscopic titration experiment where the dilution of protein solution cannot be neglected, the displacement study, and the effect of non-specific binding. Thus, with a non-linear regression program, all unknown binding parameters can be determined correctly by fitting these equations to the experimental data without any data transformation. As an example of the use of the new equations, the experimental data for receptor-insulin binding were taken from literature and reanalyzed by using a non-linear regression data analysis program.</abstract><cop>England</cop><pub>Elsevier B.V</pub><pmid>8774854</pmid><doi>10.1016/0014-5793(96)00818-6</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | FEBS letters, 1996-09, Vol.392 (3), p.245-249 |
| issn | 0014-5793 1873-3468 |
| language | eng |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; ScienceDirect Journals (5 years ago - present); EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Binding equation Binding Sites Chemistry Techniques, Analytical - methods Displacement curve Dissociation constant Insulin - chemistry Insulin - metabolism Ligand-protein interaction Ligands Models, Chemical Models, Molecular Non-linear regression Physical Phenomena Physics Proteins - chemistry Proteins - metabolism Regression Analysis Spectrum Analysis - methods Titrimetry |
| title | A novel two-site binding equation presented in terms of the total ligand concentration |
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