Myelination by transplanted human and mouse central nervous system tissue after long-term cryopreservation
Human and mouse oligodendrocytes were transplanted, after a long period of cryostorage, into newborn mouse brain. Tissue fragments were obtained from brain and spinal cord of 10-week-old human fetuses and from the periventricular zone of embryonic and newborn mouse brains. Samples were stored at -18...
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| Veröffentlicht in: | Acta neuropathologica 1996-01, Vol.91 (1), p.82-88 |
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| container_title | Acta neuropathologica |
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| creator | SEILHEAN, D GANSMÜLLER, A BARON-VAN EVERCOOREN, A GUMPEL, M LACHAPELLE, F |
| description | Human and mouse oligodendrocytes were transplanted, after a long period of cryostorage, into newborn mouse brain. Tissue fragments were obtained from brain and spinal cord of 10-week-old human fetuses and from the periventricular zone of embryonic and newborn mouse brains. Samples were stored at -180 degrees C for periods of 3 days to over 5 years. Frozen or fresh fragments were transplanted into the brains of newborn shiverer mutant mice, which are deficient in myelin basic protein (MBP). Normal myelin, produced by grafted oligodendrocytes, was detected by immunohistochemistry with an anti-MBP antiserum. The best results were obtained with isospecific grafts. The timing of myelin appearance did not depend significantly on the species or age of the donor. Myelination obtained with mouse grafts was more profuse when the donor was younger (embryonic versus newborn). Cryopreservation over 5 years did not impede the graft's ability to produce myelin and can be considered for long-term storage of oligodendrocytes in view of cell therapy. |
| doi_str_mv | 10.1007/s004010050396 |
| format | Article |
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Tissue fragments were obtained from brain and spinal cord of 10-week-old human fetuses and from the periventricular zone of embryonic and newborn mouse brains. Samples were stored at -180 degrees C for periods of 3 days to over 5 years. Frozen or fresh fragments were transplanted into the brains of newborn shiverer mutant mice, which are deficient in myelin basic protein (MBP). Normal myelin, produced by grafted oligodendrocytes, was detected by immunohistochemistry with an anti-MBP antiserum. The best results were obtained with isospecific grafts. The timing of myelin appearance did not depend significantly on the species or age of the donor. Myelination obtained with mouse grafts was more profuse when the donor was younger (embryonic versus newborn). Cryopreservation over 5 years did not impede the graft's ability to produce myelin and can be considered for long-term storage of oligodendrocytes in view of cell therapy.</description><identifier>ISSN: 0001-6322</identifier><identifier>EISSN: 1432-0533</identifier><identifier>DOI: 10.1007/s004010050396</identifier><identifier>PMID: 8773151</identifier><identifier>CODEN: ANPTAL</identifier><language>eng</language><publisher>Berlin: Springer</publisher><subject>Animals ; Animals, Newborn ; Biological and medical sciences ; Brain Tissue Transplantation ; Cryopreservation ; Embryo Transfer ; Fetal Tissue Transplantation - physiology ; Humans ; Medical sciences ; Mice ; Mice, Mutant Strains ; Myelin Basic Protein - analysis ; Myelin Sheath - physiology ; Myelin Sheath - transplantation ; Neurosurgery ; Oligodendroglia - physiology ; Oligodendroglia - transplantation ; Oligodendroglia - ultrastructure ; Skull, brain, vascular surgery ; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases ; Telencephalon - physiology ; Telencephalon - transplantation ; Telencephalon - ultrastructure</subject><ispartof>Acta neuropathologica, 1996-01, Vol.91 (1), p.82-88</ispartof><rights>1996 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c348t-67d51d5c702baabef67e1f2a4f65a9968037c5a0af912f9a6968e8c10d9e14163</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2926856$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8773151$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>SEILHEAN, D</creatorcontrib><creatorcontrib>GANSMÜLLER, A</creatorcontrib><creatorcontrib>BARON-VAN EVERCOOREN, A</creatorcontrib><creatorcontrib>GUMPEL, M</creatorcontrib><creatorcontrib>LACHAPELLE, F</creatorcontrib><title>Myelination by transplanted human and mouse central nervous system tissue after long-term cryopreservation</title><title>Acta neuropathologica</title><addtitle>Acta Neuropathol</addtitle><description>Human and mouse oligodendrocytes were transplanted, after a long period of cryostorage, into newborn mouse brain. Tissue fragments were obtained from brain and spinal cord of 10-week-old human fetuses and from the periventricular zone of embryonic and newborn mouse brains. Samples were stored at -180 degrees C for periods of 3 days to over 5 years. Frozen or fresh fragments were transplanted into the brains of newborn shiverer mutant mice, which are deficient in myelin basic protein (MBP). Normal myelin, produced by grafted oligodendrocytes, was detected by immunohistochemistry with an anti-MBP antiserum. The best results were obtained with isospecific grafts. The timing of myelin appearance did not depend significantly on the species or age of the donor. Myelination obtained with mouse grafts was more profuse when the donor was younger (embryonic versus newborn). Cryopreservation over 5 years did not impede the graft's ability to produce myelin and can be considered for long-term storage of oligodendrocytes in view of cell therapy.</description><subject>Animals</subject><subject>Animals, Newborn</subject><subject>Biological and medical sciences</subject><subject>Brain Tissue Transplantation</subject><subject>Cryopreservation</subject><subject>Embryo Transfer</subject><subject>Fetal Tissue Transplantation - physiology</subject><subject>Humans</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Mice, Mutant Strains</subject><subject>Myelin Basic Protein - analysis</subject><subject>Myelin Sheath - physiology</subject><subject>Myelin Sheath - transplantation</subject><subject>Neurosurgery</subject><subject>Oligodendroglia - physiology</subject><subject>Oligodendroglia - transplantation</subject><subject>Oligodendroglia - ultrastructure</subject><subject>Skull, brain, vascular surgery</subject><subject>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</subject><subject>Telencephalon - physiology</subject><subject>Telencephalon - transplantation</subject><subject>Telencephalon - ultrastructure</subject><issn>0001-6322</issn><issn>1432-0533</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcuL1TAUxoMo43V06VLIQtx1PEmapFnK4GNgxI2uy7npiXZo02tOK_S_NzqXAVeuzuP78XEeQrxUcKUA_FsGaKFmFkxwj8RBtUY3YI15LA4AoBpntH4qnjHf1Ur71l6Ii857o6w6iLvPO01jxnVcsjzuci2Y-TRhXmmQP7YZs8Q8yHnZmGSkXPVJZiq_akPyzivNch2ZN5KYVipyWvL3piazjGVfToW4wn_tn4snCSemF-d4Kb59eP_1-lNz--XjzfW72yaatlsb5werBhs96CPikZLzpJLGNjmLIbgOjI8WAVNQOgV0tUVdVDAEUq1y5lK8ufc9leXnRrz288iRproU1al73-nOgFX_BZW1IbTGVLC5B2NZmAul_lTGGcveK-j_PKH_5wmVf3U23o4zDQ_0-epVf33WkSNOqd48jvyA6aBdZ535DT9xkFc</recordid><startdate>19960101</startdate><enddate>19960101</enddate><creator>SEILHEAN, D</creator><creator>GANSMÜLLER, A</creator><creator>BARON-VAN EVERCOOREN, A</creator><creator>GUMPEL, M</creator><creator>LACHAPELLE, F</creator><general>Springer</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>19960101</creationdate><title>Myelination by transplanted human and mouse central nervous system tissue after long-term cryopreservation</title><author>SEILHEAN, D ; GANSMÜLLER, A ; BARON-VAN EVERCOOREN, A ; GUMPEL, M ; LACHAPELLE, F</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c348t-67d51d5c702baabef67e1f2a4f65a9968037c5a0af912f9a6968e8c10d9e14163</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Animals</topic><topic>Animals, Newborn</topic><topic>Biological and medical sciences</topic><topic>Brain Tissue Transplantation</topic><topic>Cryopreservation</topic><topic>Embryo Transfer</topic><topic>Fetal Tissue Transplantation - physiology</topic><topic>Humans</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Mice, Mutant Strains</topic><topic>Myelin Basic Protein - analysis</topic><topic>Myelin Sheath - physiology</topic><topic>Myelin Sheath - transplantation</topic><topic>Neurosurgery</topic><topic>Oligodendroglia - physiology</topic><topic>Oligodendroglia - transplantation</topic><topic>Oligodendroglia - ultrastructure</topic><topic>Skull, brain, vascular surgery</topic><topic>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</topic><topic>Telencephalon - physiology</topic><topic>Telencephalon - transplantation</topic><topic>Telencephalon - ultrastructure</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>SEILHEAN, D</creatorcontrib><creatorcontrib>GANSMÜLLER, A</creatorcontrib><creatorcontrib>BARON-VAN EVERCOOREN, A</creatorcontrib><creatorcontrib>GUMPEL, M</creatorcontrib><creatorcontrib>LACHAPELLE, F</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Acta neuropathologica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>SEILHEAN, D</au><au>GANSMÜLLER, A</au><au>BARON-VAN EVERCOOREN, A</au><au>GUMPEL, M</au><au>LACHAPELLE, F</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Myelination by transplanted human and mouse central nervous system tissue after long-term cryopreservation</atitle><jtitle>Acta neuropathologica</jtitle><addtitle>Acta Neuropathol</addtitle><date>1996-01-01</date><risdate>1996</risdate><volume>91</volume><issue>1</issue><spage>82</spage><epage>88</epage><pages>82-88</pages><issn>0001-6322</issn><eissn>1432-0533</eissn><coden>ANPTAL</coden><abstract>Human and mouse oligodendrocytes were transplanted, after a long period of cryostorage, into newborn mouse brain. Tissue fragments were obtained from brain and spinal cord of 10-week-old human fetuses and from the periventricular zone of embryonic and newborn mouse brains. Samples were stored at -180 degrees C for periods of 3 days to over 5 years. Frozen or fresh fragments were transplanted into the brains of newborn shiverer mutant mice, which are deficient in myelin basic protein (MBP). Normal myelin, produced by grafted oligodendrocytes, was detected by immunohistochemistry with an anti-MBP antiserum. The best results were obtained with isospecific grafts. The timing of myelin appearance did not depend significantly on the species or age of the donor. Myelination obtained with mouse grafts was more profuse when the donor was younger (embryonic versus newborn). Cryopreservation over 5 years did not impede the graft's ability to produce myelin and can be considered for long-term storage of oligodendrocytes in view of cell therapy.</abstract><cop>Berlin</cop><pub>Springer</pub><pmid>8773151</pmid><doi>10.1007/s004010050396</doi><tpages>7</tpages></addata></record> |
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| ispartof | Acta neuropathologica, 1996-01, Vol.91 (1), p.82-88 |
| issn | 0001-6322 1432-0533 |
| language | eng |
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| source | MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | Animals Animals, Newborn Biological and medical sciences Brain Tissue Transplantation Cryopreservation Embryo Transfer Fetal Tissue Transplantation - physiology Humans Medical sciences Mice Mice, Mutant Strains Myelin Basic Protein - analysis Myelin Sheath - physiology Myelin Sheath - transplantation Neurosurgery Oligodendroglia - physiology Oligodendroglia - transplantation Oligodendroglia - ultrastructure Skull, brain, vascular surgery Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases Telencephalon - physiology Telencephalon - transplantation Telencephalon - ultrastructure |
| title | Myelination by transplanted human and mouse central nervous system tissue after long-term cryopreservation |
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