Studies on the 240-kDa Con A-binding glycoprotein of rat cerebellum, a putative marker of synaptic junctions
A Con A-binding glycoprotein of M r 240,000 was isolated from the remaining residue of rat cerebella after sequential extraction with buffers supplemented with or without neutral detergents. It was further purified by affinity chromatography on Con A-Sepharose in the presence of sodium dodecyl sulfa...
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| Veröffentlicht in: | Brain research 1988-05, Vol.40 (2), p.193-200 |
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| creator | Bezamahouta, César Zanetta, Jean-Pierre Clos, Jean Meyer, Alphonse Vincendon, Guy |
| description | A Con A-binding glycoprotein of
M
r 240,000 was isolated from the remaining residue of rat cerebella after sequential extraction with buffers supplemented with or without neutral detergents. It was further purified by affinity chromatography on Con A-Sepharose in the presence of sodium dodecyl sulfate and preparative gel electrophoresis. This glycoprotein partially resists Triton X-100 extraction and is soluble in
N-lauryl sarcosinate. The 240-kDa glycoprotein was not detected in kidney, liver, heart, forebrain and was specifically seen in cerebellar homogenate. The isolated glycoprotein appears to be similar, not necessarily identical with the GPA — a synaptic junction 240-kDa Con A-binding glycoprotein isolated from cerebellum earlier (Groswald and Kelly,
J. Neurochem., 42 (1984) 534–546). Monospecific antibodies obtained against the purified 240-kDa protein were used for developmental study in normal and hypothyroid rats. There was observed an increase in the amount of 240-kDa glycoprotein, dependent on the age of the rat and this rise was in correlation with the synapse formation in rat cerebellum. The amount of 240-kDa glycoprotein is considerably reduced in hypothyroid rats. |
| doi_str_mv | 10.1016/0165-3806(88)90131-9 |
| format | Article |
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M
r 240,000 was isolated from the remaining residue of rat cerebella after sequential extraction with buffers supplemented with or without neutral detergents. It was further purified by affinity chromatography on Con A-Sepharose in the presence of sodium dodecyl sulfate and preparative gel electrophoresis. This glycoprotein partially resists Triton X-100 extraction and is soluble in
N-lauryl sarcosinate. The 240-kDa glycoprotein was not detected in kidney, liver, heart, forebrain and was specifically seen in cerebellar homogenate. The isolated glycoprotein appears to be similar, not necessarily identical with the GPA — a synaptic junction 240-kDa Con A-binding glycoprotein isolated from cerebellum earlier (Groswald and Kelly,
J. Neurochem., 42 (1984) 534–546). Monospecific antibodies obtained against the purified 240-kDa protein were used for developmental study in normal and hypothyroid rats. There was observed an increase in the amount of 240-kDa glycoprotein, dependent on the age of the rat and this rise was in correlation with the synapse formation in rat cerebellum. The amount of 240-kDa glycoprotein is considerably reduced in hypothyroid rats.</description><identifier>ISSN: 0165-3806</identifier><identifier>ISSN: 0006-8993</identifier><identifier>DOI: 10.1016/0165-3806(88)90131-9</identifier><identifier>PMID: 3382956</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Aging - metabolism ; Animals ; Animals, Newborn ; Antibody Specificity ; Cerebellum ; Cerebellum - growth & development ; Cerebellum - metabolism ; Development ; Glycoprotein 240-kDa ; Hypothyroidism ; Hypothyroidism - metabolism ; Molecular Weight ; Rats ; Rats, Inbred Strains ; Receptors, Concanavalin A - immunology ; Receptors, Concanavalin A - isolation & purification ; Receptors, Concanavalin A - metabolism ; Synapses - analysis</subject><ispartof>Brain research, 1988-05, Vol.40 (2), p.193-200</ispartof><rights>1988</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c425t-33b75619dcf159f58525b7224a0beec0a4d252e4270fe725cfe041b0429c23b23</citedby><cites>FETCH-LOGICAL-c425t-33b75619dcf159f58525b7224a0beec0a4d252e4270fe725cfe041b0429c23b23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3382956$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bezamahouta, César</creatorcontrib><creatorcontrib>Zanetta, Jean-Pierre</creatorcontrib><creatorcontrib>Clos, Jean</creatorcontrib><creatorcontrib>Meyer, Alphonse</creatorcontrib><creatorcontrib>Vincendon, Guy</creatorcontrib><title>Studies on the 240-kDa Con A-binding glycoprotein of rat cerebellum, a putative marker of synaptic junctions</title><title>Brain research</title><addtitle>Brain Res</addtitle><description>A Con A-binding glycoprotein of
M
r 240,000 was isolated from the remaining residue of rat cerebella after sequential extraction with buffers supplemented with or without neutral detergents. It was further purified by affinity chromatography on Con A-Sepharose in the presence of sodium dodecyl sulfate and preparative gel electrophoresis. This glycoprotein partially resists Triton X-100 extraction and is soluble in
N-lauryl sarcosinate. The 240-kDa glycoprotein was not detected in kidney, liver, heart, forebrain and was specifically seen in cerebellar homogenate. The isolated glycoprotein appears to be similar, not necessarily identical with the GPA — a synaptic junction 240-kDa Con A-binding glycoprotein isolated from cerebellum earlier (Groswald and Kelly,
J. Neurochem., 42 (1984) 534–546). Monospecific antibodies obtained against the purified 240-kDa protein were used for developmental study in normal and hypothyroid rats. There was observed an increase in the amount of 240-kDa glycoprotein, dependent on the age of the rat and this rise was in correlation with the synapse formation in rat cerebellum. The amount of 240-kDa glycoprotein is considerably reduced in hypothyroid rats.</description><subject>Aging - metabolism</subject><subject>Animals</subject><subject>Animals, Newborn</subject><subject>Antibody Specificity</subject><subject>Cerebellum</subject><subject>Cerebellum - growth & development</subject><subject>Cerebellum - metabolism</subject><subject>Development</subject><subject>Glycoprotein 240-kDa</subject><subject>Hypothyroidism</subject><subject>Hypothyroidism - metabolism</subject><subject>Molecular Weight</subject><subject>Rats</subject><subject>Rats, Inbred Strains</subject><subject>Receptors, Concanavalin A - immunology</subject><subject>Receptors, Concanavalin A - isolation & purification</subject><subject>Receptors, Concanavalin A - metabolism</subject><subject>Synapses - analysis</subject><issn>0165-3806</issn><issn>0006-8993</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1988</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kF1LwzAUhnOhzDn9Bwq5EgWrSdq06Y0w5icMvFCvQ5qezmxdOpN0sH9v5sYuvTiEk_c9Xw9CF5TcUULz-xg8SQXJr4W4KQlNaVIeoeHh-wSdej8nJCqCDtAgTQUreT5E7UfoawMedxaHb8AsI8niUeFJzMdJZWxt7AzP2o3uVq4LYCzuGuxUwBocVNC2_fIWK7zqgwpmDXip3ALc1uQ3Vq2C0XjeWx1MZ_0ZOm5U6-F8_47Q1_PT5-Q1mb6_vE3G00RnjIckTauC57SsdUN52XDBGa8KxjJFKgBNVFYzziBjBWmgYFw3QDJakYyVmqUVS0foatc3rvzTgw9yabyOuyoLXe9lIVgucsqjMdsZteu8d9DIlTPxgo2kRG7Byi1BuSUohZB_YGUZyy73_ftqCfWhaE816g87HeKRawNOem3AaqiNAx1k3Zn_B_wC0RKJCg</recordid><startdate>19880516</startdate><enddate>19880516</enddate><creator>Bezamahouta, César</creator><creator>Zanetta, Jean-Pierre</creator><creator>Clos, Jean</creator><creator>Meyer, Alphonse</creator><creator>Vincendon, Guy</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19880516</creationdate><title>Studies on the 240-kDa Con A-binding glycoprotein of rat cerebellum, a putative marker of synaptic junctions</title><author>Bezamahouta, César ; Zanetta, Jean-Pierre ; Clos, Jean ; Meyer, Alphonse ; Vincendon, Guy</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c425t-33b75619dcf159f58525b7224a0beec0a4d252e4270fe725cfe041b0429c23b23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1988</creationdate><topic>Aging - metabolism</topic><topic>Animals</topic><topic>Animals, Newborn</topic><topic>Antibody Specificity</topic><topic>Cerebellum</topic><topic>Cerebellum - growth & development</topic><topic>Cerebellum - metabolism</topic><topic>Development</topic><topic>Glycoprotein 240-kDa</topic><topic>Hypothyroidism</topic><topic>Hypothyroidism - metabolism</topic><topic>Molecular Weight</topic><topic>Rats</topic><topic>Rats, Inbred Strains</topic><topic>Receptors, Concanavalin A - immunology</topic><topic>Receptors, Concanavalin A - isolation & purification</topic><topic>Receptors, Concanavalin A - metabolism</topic><topic>Synapses - analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bezamahouta, César</creatorcontrib><creatorcontrib>Zanetta, Jean-Pierre</creatorcontrib><creatorcontrib>Clos, Jean</creatorcontrib><creatorcontrib>Meyer, Alphonse</creatorcontrib><creatorcontrib>Vincendon, Guy</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Brain research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bezamahouta, César</au><au>Zanetta, Jean-Pierre</au><au>Clos, Jean</au><au>Meyer, Alphonse</au><au>Vincendon, Guy</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Studies on the 240-kDa Con A-binding glycoprotein of rat cerebellum, a putative marker of synaptic junctions</atitle><jtitle>Brain research</jtitle><addtitle>Brain Res</addtitle><date>1988-05-16</date><risdate>1988</risdate><volume>40</volume><issue>2</issue><spage>193</spage><epage>200</epage><pages>193-200</pages><issn>0165-3806</issn><issn>0006-8993</issn><abstract>A Con A-binding glycoprotein of
M
r 240,000 was isolated from the remaining residue of rat cerebella after sequential extraction with buffers supplemented with or without neutral detergents. It was further purified by affinity chromatography on Con A-Sepharose in the presence of sodium dodecyl sulfate and preparative gel electrophoresis. This glycoprotein partially resists Triton X-100 extraction and is soluble in
N-lauryl sarcosinate. The 240-kDa glycoprotein was not detected in kidney, liver, heart, forebrain and was specifically seen in cerebellar homogenate. The isolated glycoprotein appears to be similar, not necessarily identical with the GPA — a synaptic junction 240-kDa Con A-binding glycoprotein isolated from cerebellum earlier (Groswald and Kelly,
J. Neurochem., 42 (1984) 534–546). Monospecific antibodies obtained against the purified 240-kDa protein were used for developmental study in normal and hypothyroid rats. There was observed an increase in the amount of 240-kDa glycoprotein, dependent on the age of the rat and this rise was in correlation with the synapse formation in rat cerebellum. The amount of 240-kDa glycoprotein is considerably reduced in hypothyroid rats.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>3382956</pmid><doi>10.1016/0165-3806(88)90131-9</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0165-3806 |
| ispartof | Brain research, 1988-05, Vol.40 (2), p.193-200 |
| issn | 0165-3806 0006-8993 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_78268615 |
| source | MEDLINE; Access via ScienceDirect (Elsevier); Alma/SFX Local Collection |
| subjects | Aging - metabolism Animals Animals, Newborn Antibody Specificity Cerebellum Cerebellum - growth & development Cerebellum - metabolism Development Glycoprotein 240-kDa Hypothyroidism Hypothyroidism - metabolism Molecular Weight Rats Rats, Inbred Strains Receptors, Concanavalin A - immunology Receptors, Concanavalin A - isolation & purification Receptors, Concanavalin A - metabolism Synapses - analysis |
| title | Studies on the 240-kDa Con A-binding glycoprotein of rat cerebellum, a putative marker of synaptic junctions |
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