Identification of alpha 2-adrenergic receptors in chicken pineal gland using [3H]rauwolscine
The norepinephrine-induced inhibition of avian pineal N-acetyltransferase activity appears to be mediated by alpha 2-adrenergic receptors. In this study, alpha 2-adrenergic receptors in the chicken pineal gland were directly identified by radioligand binding. Membrane preparations of pineal glands f...
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| Veröffentlicht in: | Journal of neurochemistry 1988-07, Vol.51 (1), p.81-86 |
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| container_title | Journal of neurochemistry |
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| creator | Bylund, D B Rudeen, P K Petterborg, L J Ray-Prenger, C |
| description | The norepinephrine-induced inhibition of avian pineal N-acetyltransferase activity appears to be mediated by alpha 2-adrenergic receptors. In this study, alpha 2-adrenergic receptors in the chicken pineal gland were directly identified by radioligand binding. Membrane preparations of pineal glands from chickens from 1 to 6 weeks of age were examined using [3H]rauwolscine, a selective alpha 2-adrenergic receptor antagonist, to characterize the binding sites. The results indicate no ontological change in either the affinity (KD) or density of receptor binding sites (Bmax) during the time span examined. The binding was saturable and of high affinity with a mean KD of 0.27 +/- 0.01 nM and a mean Bmax of 242 +/- 12 fmol/mg protein. Further characterization of these binding sites indicated that the alpha 2-adrenergic receptor is of the alpha 2A subtype, since prazosin and ARC-239 bound with low affinities and oxymetazoline bound with high affinity. |
| doi_str_mv | 10.1111/j.1471-4159.1988.tb04838.x |
| format | Article |
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In this study, alpha 2-adrenergic receptors in the chicken pineal gland were directly identified by radioligand binding. Membrane preparations of pineal glands from chickens from 1 to 6 weeks of age were examined using [3H]rauwolscine, a selective alpha 2-adrenergic receptor antagonist, to characterize the binding sites. The results indicate no ontological change in either the affinity (KD) or density of receptor binding sites (Bmax) during the time span examined. The binding was saturable and of high affinity with a mean KD of 0.27 +/- 0.01 nM and a mean Bmax of 242 +/- 12 fmol/mg protein. 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In this study, alpha 2-adrenergic receptors in the chicken pineal gland were directly identified by radioligand binding. Membrane preparations of pineal glands from chickens from 1 to 6 weeks of age were examined using [3H]rauwolscine, a selective alpha 2-adrenergic receptor antagonist, to characterize the binding sites. The results indicate no ontological change in either the affinity (KD) or density of receptor binding sites (Bmax) during the time span examined. The binding was saturable and of high affinity with a mean KD of 0.27 +/- 0.01 nM and a mean Bmax of 242 +/- 12 fmol/mg protein. Further characterization of these binding sites indicated that the alpha 2-adrenergic receptor is of the alpha 2A subtype, since prazosin and ARC-239 bound with low affinities and oxymetazoline bound with high affinity.</description><subject>Adrenergic alpha-Antagonists</subject><subject>Animals</subject><subject>Brimonidine Tartrate</subject><subject>Cell Membrane - metabolism</subject><subject>Chickens - metabolism</subject><subject>Circadian Rhythm</subject><subject>Female</subject><subject>Isoquinolines - metabolism</subject><subject>Isoquinolines - pharmacology</subject><subject>Male</subject><subject>Pindolol - metabolism</subject><subject>Pineal Gland - metabolism</subject><subject>Piperazines</subject><subject>Quinoxalines - metabolism</subject><subject>Quinoxalines - pharmacology</subject><subject>Receptors, Adrenergic, alpha - drug effects</subject><subject>Receptors, Adrenergic, alpha - metabolism</subject><subject>Tritium</subject><subject>Yohimbine - metabolism</subject><subject>Yohimbine - pharmacology</subject><issn>0022-3042</issn><issn>1471-4159</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1988</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kE1Lw0AQhhdRaq3-BGHx4C1xv7LZeJOitlDwoieVZb_Sbk2TuJtg_fcmtDiXYXjfd2Z4ALjBKMVD3W1TzHKcMJwVKS6ESDuNmKAi3Z-A6b90CqYIEZJQxMg5uIhxixDmjOMJmBBRCITEFHwsras7X3qjOt_UsCmhqtqNgiRRNrjahbU3MDjj2q4JEfoamo03X66Gra-dquC6UrWFffT1Gr7TxWdQ_U9TRTOol-CsVFV0V8c-A29Pj6_zRbJ6eV7OH1aJIYx3Cc8o0qXgwmruFLPM5AqXzBAnhFbMZFZpY4ucc4Mpw7qw1BJTsFIPc1lkdAZuD3vb0Hz3LnZy56Nx1fCZa_ooc0F4xpEYjPcHowlNjMGVsg1-p8KvxEiOaOVWjvzkyE-OaOURrdwP4evjlV7vnP2PHlnSP1BLeB0</recordid><startdate>198807</startdate><enddate>198807</enddate><creator>Bylund, D B</creator><creator>Rudeen, P K</creator><creator>Petterborg, L J</creator><creator>Ray-Prenger, C</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>198807</creationdate><title>Identification of alpha 2-adrenergic receptors in chicken pineal gland using [3H]rauwolscine</title><author>Bylund, D B ; Rudeen, P K ; Petterborg, L J ; Ray-Prenger, C</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c246t-6530bf868db6ea4d4c7a1f4c2e88ba4c5dabcd9766c1341b9d3d2c94fbc13f953</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1988</creationdate><topic>Adrenergic alpha-Antagonists</topic><topic>Animals</topic><topic>Brimonidine Tartrate</topic><topic>Cell Membrane - metabolism</topic><topic>Chickens - metabolism</topic><topic>Circadian Rhythm</topic><topic>Female</topic><topic>Isoquinolines - metabolism</topic><topic>Isoquinolines - pharmacology</topic><topic>Male</topic><topic>Pindolol - metabolism</topic><topic>Pineal Gland - metabolism</topic><topic>Piperazines</topic><topic>Quinoxalines - metabolism</topic><topic>Quinoxalines - pharmacology</topic><topic>Receptors, Adrenergic, alpha - drug effects</topic><topic>Receptors, Adrenergic, alpha - metabolism</topic><topic>Tritium</topic><topic>Yohimbine - metabolism</topic><topic>Yohimbine - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bylund, D B</creatorcontrib><creatorcontrib>Rudeen, P K</creatorcontrib><creatorcontrib>Petterborg, L J</creatorcontrib><creatorcontrib>Ray-Prenger, C</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of neurochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bylund, D B</au><au>Rudeen, P K</au><au>Petterborg, L J</au><au>Ray-Prenger, C</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of alpha 2-adrenergic receptors in chicken pineal gland using [3H]rauwolscine</atitle><jtitle>Journal of neurochemistry</jtitle><addtitle>J Neurochem</addtitle><date>1988-07</date><risdate>1988</risdate><volume>51</volume><issue>1</issue><spage>81</spage><epage>86</epage><pages>81-86</pages><issn>0022-3042</issn><eissn>1471-4159</eissn><abstract>The norepinephrine-induced inhibition of avian pineal N-acetyltransferase activity appears to be mediated by alpha 2-adrenergic receptors. In this study, alpha 2-adrenergic receptors in the chicken pineal gland were directly identified by radioligand binding. Membrane preparations of pineal glands from chickens from 1 to 6 weeks of age were examined using [3H]rauwolscine, a selective alpha 2-adrenergic receptor antagonist, to characterize the binding sites. The results indicate no ontological change in either the affinity (KD) or density of receptor binding sites (Bmax) during the time span examined. The binding was saturable and of high affinity with a mean KD of 0.27 +/- 0.01 nM and a mean Bmax of 242 +/- 12 fmol/mg protein. Further characterization of these binding sites indicated that the alpha 2-adrenergic receptor is of the alpha 2A subtype, since prazosin and ARC-239 bound with low affinities and oxymetazoline bound with high affinity.</abstract><cop>England</cop><pmid>2898008</pmid><doi>10.1111/j.1471-4159.1988.tb04838.x</doi><tpages>6</tpages></addata></record> |
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| identifier | ISSN: 0022-3042 |
| ispartof | Journal of neurochemistry, 1988-07, Vol.51 (1), p.81-86 |
| issn | 0022-3042 1471-4159 |
| language | eng |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Adrenergic alpha-Antagonists Animals Brimonidine Tartrate Cell Membrane - metabolism Chickens - metabolism Circadian Rhythm Female Isoquinolines - metabolism Isoquinolines - pharmacology Male Pindolol - metabolism Pineal Gland - metabolism Piperazines Quinoxalines - metabolism Quinoxalines - pharmacology Receptors, Adrenergic, alpha - drug effects Receptors, Adrenergic, alpha - metabolism Tritium Yohimbine - metabolism Yohimbine - pharmacology |
| title | Identification of alpha 2-adrenergic receptors in chicken pineal gland using [3H]rauwolscine |
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