Studies on the effect of tetrandrine on microtubules: I. Biochemical Observation and electron microscopy
Microtubules were purified by using two cycles of assembly and disassembly processes on fresh brain homogenates from 30 guinea pigs. The yield was about 60 mg. The effect of tetrandrine on tubulin was determined by spectrophotometric analysis and electron microscopy. In addition, we used the indirec...
Gespeichert in:
| Veröffentlicht in: | Ecotoxicology and environmental safety 1988, Vol.15 (2), p.142-148 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 148 |
|---|---|
| container_issue | 2 |
| container_start_page | 142 |
| container_title | Ecotoxicology and environmental safety |
| container_volume | 15 |
| creator | Liu, Lingfen Chen, Ningmeng Cai, Guoping Li, Zhaolin Yang, Jigong Li, Yurui |
| description | Microtubules were purified by using two cycles of assembly and disassembly processes on fresh brain homogenates from 30 guinea pigs. The yield was about 60 mg. The effect of tetrandrine on tubulin was determined by spectrophotometric analysis and electron microscopy. In addition, we used the indirect immunofluorescent method including tubulin antibody to locate the presence of microtubules in 3T3 cells by fluorescence microscopy. The effects of colchicine and P
204 were studied for comparison at the same time. The results showed that colchicine can effectively depolymerize microtubules, while tetrandrine showed aggregation, and in a different manner. The shape and structure of microtubules were definitely destroyed by colchicine, but were not affected by P
204 which protected against the destructive effect of tetrandrine. This result indicated the safety of using a combination of P
204 and tetrandrine in the treatment of silicosis. |
| doi_str_mv | 10.1016/0147-6513(88)90065-6 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_miscellaneous_78264901</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>0147651388900656</els_id><sourcerecordid>78264901</sourcerecordid><originalsourceid>FETCH-LOGICAL-e236t-e0025efc7cf1d93722284909c4eef459024cb42889b26df23cc879f329a960c23</originalsourceid><addsrcrecordid>eNqFkU9LHTEUxUOp2KftN2ghiyK6GL35M5nEhdCKrYLgou06zGRueJF5M88kI_jtzeirW1cX7vndA_ccQr4yOGXA1Bkw2VSqZuJY6xMDoOpKfSArBgYqLpn8SFZvyCdykNI9AAio632yL0SjayFXZP0nz33ARKeR5jVS9B5dppOnGXNsxz6GERdxE1yc8tzNA6ZzenNKf4bJrbGs24HedQnjY5tDAcsNxaGYxP9XyU3bp89kz7dDwi-7eUj-_br6e3ld3d79vrn8cVshFypXCMBr9K5xnvVGNJxzLQ0YJxG9rA1w6TrJtTYdV73nwjndGC-4aY0Cx8UhOXr13cbpYcaU7SYkh8PQjjjNyTaaq2LI3gWZNFKCNgX8tgPnboO93cawaeOT3WVY9O87vU0lDF9ScyG9YQ1IpdSCXbxiWL5_DBhtcgFHh32IJS3bT8EysEu1dunNLr1Zre1LtVaJZ6GplJU</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>14944089</pqid></control><display><type>article</type><title>Studies on the effect of tetrandrine on microtubules: I. Biochemical Observation and electron microscopy</title><source>MEDLINE</source><source>Access via ScienceDirect (Elsevier)</source><creator>Liu, Lingfen ; Chen, Ningmeng ; Cai, Guoping ; Li, Zhaolin ; Yang, Jigong ; Li, Yurui</creator><creatorcontrib>Liu, Lingfen ; Chen, Ningmeng ; Cai, Guoping ; Li, Zhaolin ; Yang, Jigong ; Li, Yurui</creatorcontrib><description>Microtubules were purified by using two cycles of assembly and disassembly processes on fresh brain homogenates from 30 guinea pigs. The yield was about 60 mg. The effect of tetrandrine on tubulin was determined by spectrophotometric analysis and electron microscopy. In addition, we used the indirect immunofluorescent method including tubulin antibody to locate the presence of microtubules in 3T3 cells by fluorescence microscopy. The effects of colchicine and P
204 were studied for comparison at the same time. The results showed that colchicine can effectively depolymerize microtubules, while tetrandrine showed aggregation, and in a different manner. The shape and structure of microtubules were definitely destroyed by colchicine, but were not affected by P
204 which protected against the destructive effect of tetrandrine. This result indicated the safety of using a combination of P
204 and tetrandrine in the treatment of silicosis.</description><identifier>ISSN: 0147-6513</identifier><identifier>EISSN: 1090-2414</identifier><identifier>DOI: 10.1016/0147-6513(88)90065-6</identifier><identifier>PMID: 3378534</identifier><identifier>CODEN: EESADV</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>Alkaloids - pharmacology ; Animals ; Benzylisoquinolines ; Biological and medical sciences ; Brain - drug effects ; Brain - ultrastructure ; Colchicine - pharmacology ; Drug toxicity and drugs side effects treatment ; Electrophoresis, Polyacrylamide Gel ; Guinea Pigs ; Medical sciences ; Microscopy, Electron ; Microtubules - analysis ; Microtubules - drug effects ; Microtubules - ultrastructure ; Pharmacology. Drug treatments ; Spectrophotometry ; Toxicity: nervous system and muscle ; Tubulin - metabolism</subject><ispartof>Ecotoxicology and environmental safety, 1988, Vol.15 (2), p.142-148</ispartof><rights>1988</rights><rights>1989 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0147-6513(88)90065-6$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,781,785,3551,4025,27925,27926,27927,45997</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7046664$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3378534$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Liu, Lingfen</creatorcontrib><creatorcontrib>Chen, Ningmeng</creatorcontrib><creatorcontrib>Cai, Guoping</creatorcontrib><creatorcontrib>Li, Zhaolin</creatorcontrib><creatorcontrib>Yang, Jigong</creatorcontrib><creatorcontrib>Li, Yurui</creatorcontrib><title>Studies on the effect of tetrandrine on microtubules: I. Biochemical Observation and electron microscopy</title><title>Ecotoxicology and environmental safety</title><addtitle>Ecotoxicol Environ Saf</addtitle><description>Microtubules were purified by using two cycles of assembly and disassembly processes on fresh brain homogenates from 30 guinea pigs. The yield was about 60 mg. The effect of tetrandrine on tubulin was determined by spectrophotometric analysis and electron microscopy. In addition, we used the indirect immunofluorescent method including tubulin antibody to locate the presence of microtubules in 3T3 cells by fluorescence microscopy. The effects of colchicine and P
204 were studied for comparison at the same time. The results showed that colchicine can effectively depolymerize microtubules, while tetrandrine showed aggregation, and in a different manner. The shape and structure of microtubules were definitely destroyed by colchicine, but were not affected by P
204 which protected against the destructive effect of tetrandrine. This result indicated the safety of using a combination of P
204 and tetrandrine in the treatment of silicosis.</description><subject>Alkaloids - pharmacology</subject><subject>Animals</subject><subject>Benzylisoquinolines</subject><subject>Biological and medical sciences</subject><subject>Brain - drug effects</subject><subject>Brain - ultrastructure</subject><subject>Colchicine - pharmacology</subject><subject>Drug toxicity and drugs side effects treatment</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Guinea Pigs</subject><subject>Medical sciences</subject><subject>Microscopy, Electron</subject><subject>Microtubules - analysis</subject><subject>Microtubules - drug effects</subject><subject>Microtubules - ultrastructure</subject><subject>Pharmacology. Drug treatments</subject><subject>Spectrophotometry</subject><subject>Toxicity: nervous system and muscle</subject><subject>Tubulin - metabolism</subject><issn>0147-6513</issn><issn>1090-2414</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1988</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU9LHTEUxUOp2KftN2ghiyK6GL35M5nEhdCKrYLgou06zGRueJF5M88kI_jtzeirW1cX7vndA_ccQr4yOGXA1Bkw2VSqZuJY6xMDoOpKfSArBgYqLpn8SFZvyCdykNI9AAio632yL0SjayFXZP0nz33ARKeR5jVS9B5dppOnGXNsxz6GERdxE1yc8tzNA6ZzenNKf4bJrbGs24HedQnjY5tDAcsNxaGYxP9XyU3bp89kz7dDwi-7eUj-_br6e3ld3d79vrn8cVshFypXCMBr9K5xnvVGNJxzLQ0YJxG9rA1w6TrJtTYdV73nwjndGC-4aY0Cx8UhOXr13cbpYcaU7SYkh8PQjjjNyTaaq2LI3gWZNFKCNgX8tgPnboO93cawaeOT3WVY9O87vU0lDF9ScyG9YQ1IpdSCXbxiWL5_DBhtcgFHh32IJS3bT8EysEu1dunNLr1Zre1LtVaJZ6GplJU</recordid><startdate>1988</startdate><enddate>1988</enddate><creator>Liu, Lingfen</creator><creator>Chen, Ningmeng</creator><creator>Cai, Guoping</creator><creator>Li, Zhaolin</creator><creator>Yang, Jigong</creator><creator>Li, Yurui</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>1988</creationdate><title>Studies on the effect of tetrandrine on microtubules: I. Biochemical Observation and electron microscopy</title><author>Liu, Lingfen ; Chen, Ningmeng ; Cai, Guoping ; Li, Zhaolin ; Yang, Jigong ; Li, Yurui</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-e236t-e0025efc7cf1d93722284909c4eef459024cb42889b26df23cc879f329a960c23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1988</creationdate><topic>Alkaloids - pharmacology</topic><topic>Animals</topic><topic>Benzylisoquinolines</topic><topic>Biological and medical sciences</topic><topic>Brain - drug effects</topic><topic>Brain - ultrastructure</topic><topic>Colchicine - pharmacology</topic><topic>Drug toxicity and drugs side effects treatment</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Guinea Pigs</topic><topic>Medical sciences</topic><topic>Microscopy, Electron</topic><topic>Microtubules - analysis</topic><topic>Microtubules - drug effects</topic><topic>Microtubules - ultrastructure</topic><topic>Pharmacology. Drug treatments</topic><topic>Spectrophotometry</topic><topic>Toxicity: nervous system and muscle</topic><topic>Tubulin - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liu, Lingfen</creatorcontrib><creatorcontrib>Chen, Ningmeng</creatorcontrib><creatorcontrib>Cai, Guoping</creatorcontrib><creatorcontrib>Li, Zhaolin</creatorcontrib><creatorcontrib>Yang, Jigong</creatorcontrib><creatorcontrib>Li, Yurui</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Ecotoxicology and environmental safety</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, Lingfen</au><au>Chen, Ningmeng</au><au>Cai, Guoping</au><au>Li, Zhaolin</au><au>Yang, Jigong</au><au>Li, Yurui</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Studies on the effect of tetrandrine on microtubules: I. Biochemical Observation and electron microscopy</atitle><jtitle>Ecotoxicology and environmental safety</jtitle><addtitle>Ecotoxicol Environ Saf</addtitle><date>1988</date><risdate>1988</risdate><volume>15</volume><issue>2</issue><spage>142</spage><epage>148</epage><pages>142-148</pages><issn>0147-6513</issn><eissn>1090-2414</eissn><coden>EESADV</coden><abstract>Microtubules were purified by using two cycles of assembly and disassembly processes on fresh brain homogenates from 30 guinea pigs. The yield was about 60 mg. The effect of tetrandrine on tubulin was determined by spectrophotometric analysis and electron microscopy. In addition, we used the indirect immunofluorescent method including tubulin antibody to locate the presence of microtubules in 3T3 cells by fluorescence microscopy. The effects of colchicine and P
204 were studied for comparison at the same time. The results showed that colchicine can effectively depolymerize microtubules, while tetrandrine showed aggregation, and in a different manner. The shape and structure of microtubules were definitely destroyed by colchicine, but were not affected by P
204 which protected against the destructive effect of tetrandrine. This result indicated the safety of using a combination of P
204 and tetrandrine in the treatment of silicosis.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>3378534</pmid><doi>10.1016/0147-6513(88)90065-6</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0147-6513 |
| ispartof | Ecotoxicology and environmental safety, 1988, Vol.15 (2), p.142-148 |
| issn | 0147-6513 1090-2414 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_78264901 |
| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Alkaloids - pharmacology Animals Benzylisoquinolines Biological and medical sciences Brain - drug effects Brain - ultrastructure Colchicine - pharmacology Drug toxicity and drugs side effects treatment Electrophoresis, Polyacrylamide Gel Guinea Pigs Medical sciences Microscopy, Electron Microtubules - analysis Microtubules - drug effects Microtubules - ultrastructure Pharmacology. Drug treatments Spectrophotometry Toxicity: nervous system and muscle Tubulin - metabolism |
| title | Studies on the effect of tetrandrine on microtubules: I. Biochemical Observation and electron microscopy |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-18T06%3A06%3A41IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Studies%20on%20the%20effect%20of%20tetrandrine%20on%20microtubules:%20I.%20Biochemical%20Observation%20and%20electron%20microscopy&rft.jtitle=Ecotoxicology%20and%20environmental%20safety&rft.au=Liu,%20Lingfen&rft.date=1988&rft.volume=15&rft.issue=2&rft.spage=142&rft.epage=148&rft.pages=142-148&rft.issn=0147-6513&rft.eissn=1090-2414&rft.coden=EESADV&rft_id=info:doi/10.1016/0147-6513(88)90065-6&rft_dat=%3Cproquest_pubme%3E78264901%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=14944089&rft_id=info:pmid/3378534&rft_els_id=0147651388900656&rfr_iscdi=true |