Pectic polysaccharides from roots of Glycyrrhiza uralensis: Possible contribution of neutral oligosaccharides in the galacturonase-resistant region to anti-complementary and mitogenic activities
Digestion with endo-alpha-(1-4)-polygalacturonase liberated the enzyme-resistant region (PG-1c) as an active site of the anti-complementary and mitogenic pectic polysaccharide (GR-22c) from Glycyrrhiza uralensis. Partial acid hydrolysis of PG-1c resulted in acidic oligosaccharides, and methylation a...
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| Veröffentlicht in: | Planta medica 1996-02, Vol.62 (1), p.14-19 |
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| creator | Kiyohara, H Takemoto, N Zhao, J.F Kawamura, H Yamada, H. (Oriental Medicine Research Center of the Kitasato Institute, Tokyo (Japan)) |
| description | Digestion with endo-alpha-(1-4)-polygalacturonase liberated the enzyme-resistant region (PG-1c) as an active site of the anti-complementary and mitogenic pectic polysaccharide (GR-22c) from Glycyrrhiza uralensis. Partial acid hydrolysis of PG-1c resulted in acidic oligosaccharides, and methylation analysis and malto-oligosaccharide-alditols, and malto-, GC-MS analysis of the acidic oligosaccharides suggested that PG-1c comprised a rhamnogalacturonan core as the acidic moiety. Degradation of uronic acids by lithium decreased the anti-complementary and mitogenic activities of PG-1c. Although the products from PG-1c were still active, the methylglycoside of alpha-L-Rha-(1-4)-alpha-D-GalA-(1-2)-alpha-L-Rha-(1-4)-alpha -D-GalA did not show both activities. The products obtained by the lithium degradation from PG-1c gave fractions containing various neutral oligosaccharide-alditols. Among these fractions the longest and the short oligosaccharide-alditol fraktions had relatively potent anti-complementary activity, whereas all oligosaccharide-alditol fractions expressed weak but significant mitogenic activity. GC-MS analysis indicated that the short oligosaccharide-alditol fraction contained various kinds of di- to tetrasaccharide-alditols. However, malto-oligosaccharide-alditols, and malto-, isomalto-, and laminari-oligosaccharides did not show anti-complementary and/or mitogenic activities, and these results suggested that certain neutral carbohydrate chains in PG-1c were responsible for the expression of mitogenic activity as well as anti-complementary activity of PG-1c. |
| doi_str_mv | 10.1055/s-2006-957787 |
| format | Article |
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(Oriental Medicine Research Center of the Kitasato Institute, Tokyo (Japan))</creator><creatorcontrib>Kiyohara, H ; Takemoto, N ; Zhao, J.F ; Kawamura, H ; Yamada, H. (Oriental Medicine Research Center of the Kitasato Institute, Tokyo (Japan))</creatorcontrib><description>Digestion with endo-alpha-(1-4)-polygalacturonase liberated the enzyme-resistant region (PG-1c) as an active site of the anti-complementary and mitogenic pectic polysaccharide (GR-22c) from Glycyrrhiza uralensis. Partial acid hydrolysis of PG-1c resulted in acidic oligosaccharides, and methylation analysis and malto-oligosaccharide-alditols, and malto-, GC-MS analysis of the acidic oligosaccharides suggested that PG-1c comprised a rhamnogalacturonan core as the acidic moiety. Degradation of uronic acids by lithium decreased the anti-complementary and mitogenic activities of PG-1c. Although the products from PG-1c were still active, the methylglycoside of alpha-L-Rha-(1-4)-alpha-D-GalA-(1-2)-alpha-L-Rha-(1-4)-alpha -D-GalA did not show both activities. The products obtained by the lithium degradation from PG-1c gave fractions containing various neutral oligosaccharide-alditols. Among these fractions the longest and the short oligosaccharide-alditol fraktions had relatively potent anti-complementary activity, whereas all oligosaccharide-alditol fractions expressed weak but significant mitogenic activity. GC-MS analysis indicated that the short oligosaccharide-alditol fraction contained various kinds of di- to tetrasaccharide-alditols. However, malto-oligosaccharide-alditols, and malto-, isomalto-, and laminari-oligosaccharides did not show anti-complementary and/or mitogenic activities, and these results suggested that certain neutral carbohydrate chains in PG-1c were responsible for the expression of mitogenic activity as well as anti-complementary activity of PG-1c.</description><identifier>ISSN: 0032-0943</identifier><identifier>EISSN: 1439-0221</identifier><identifier>DOI: 10.1055/s-2006-957787</identifier><identifier>PMID: 8720381</identifier><language>eng</language><publisher>Germany</publisher><subject>Animals ; Carbohydrate Sequence ; Cells, Cultured ; Complement Inactivator Proteins - pharmacology ; COMPOSICION QUIMICA ; COMPOSITION CHIMIQUE ; ESTRUCTURA QUIMICA ; FARMACOLOGIA ; Female ; Gas Chromatography-Mass Spectrometry ; GLYCYRRHIZA ; Hydrogen-Ion Concentration ; Hydrolysis ; Mice ; Mice, Inbred BALB C ; Mitogens - pharmacology ; Molecular Sequence Data ; PECTINAS ; PECTINE ; PHARMACOLOGIE ; Plant Roots - chemistry ; PLANTAS MEDICINALES ; PLANTE MEDICINALE ; POLISACARIDOS ; Polygalacturonase - metabolism ; POLYHOLOSIDE ; Polysaccharides - chemistry ; Polysaccharides - metabolism ; Polysaccharides - pharmacology ; RACINE ; RAICES ; STRUCTURE CHIMIQUE</subject><ispartof>Planta medica, 1996-02, Vol.62 (1), p.14-19</ispartof><rights>Georg Thieme Verlag Stuttgart · New York</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c344t-e7381e3d1dfb0ecc7380cbe8cca08ad44fa7d9c31cd485d3bb0e5f41ca62c3123</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.thieme-connect.de/products/ejournals/pdf/10.1055/s-2006-957787.pdf$$EPDF$$P50$$Gthieme$$H</linktopdf><link.rule.ids>314,780,784,3015,3016,27923,27924,54558</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8720381$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kiyohara, H</creatorcontrib><creatorcontrib>Takemoto, N</creatorcontrib><creatorcontrib>Zhao, J.F</creatorcontrib><creatorcontrib>Kawamura, H</creatorcontrib><creatorcontrib>Yamada, H. (Oriental Medicine Research Center of the Kitasato Institute, Tokyo (Japan))</creatorcontrib><title>Pectic polysaccharides from roots of Glycyrrhiza uralensis: Possible contribution of neutral oligosaccharides in the galacturonase-resistant region to anti-complementary and mitogenic activities</title><title>Planta medica</title><addtitle>Planta Med</addtitle><description>Digestion with endo-alpha-(1-4)-polygalacturonase liberated the enzyme-resistant region (PG-1c) as an active site of the anti-complementary and mitogenic pectic polysaccharide (GR-22c) from Glycyrrhiza uralensis. Partial acid hydrolysis of PG-1c resulted in acidic oligosaccharides, and methylation analysis and malto-oligosaccharide-alditols, and malto-, GC-MS analysis of the acidic oligosaccharides suggested that PG-1c comprised a rhamnogalacturonan core as the acidic moiety. Degradation of uronic acids by lithium decreased the anti-complementary and mitogenic activities of PG-1c. Although the products from PG-1c were still active, the methylglycoside of alpha-L-Rha-(1-4)-alpha-D-GalA-(1-2)-alpha-L-Rha-(1-4)-alpha -D-GalA did not show both activities. The products obtained by the lithium degradation from PG-1c gave fractions containing various neutral oligosaccharide-alditols. Among these fractions the longest and the short oligosaccharide-alditol fraktions had relatively potent anti-complementary activity, whereas all oligosaccharide-alditol fractions expressed weak but significant mitogenic activity. GC-MS analysis indicated that the short oligosaccharide-alditol fraction contained various kinds of di- to tetrasaccharide-alditols. However, malto-oligosaccharide-alditols, and malto-, isomalto-, and laminari-oligosaccharides did not show anti-complementary and/or mitogenic activities, and these results suggested that certain neutral carbohydrate chains in PG-1c were responsible for the expression of mitogenic activity as well as anti-complementary activity of PG-1c.</description><subject>Animals</subject><subject>Carbohydrate Sequence</subject><subject>Cells, Cultured</subject><subject>Complement Inactivator Proteins - pharmacology</subject><subject>COMPOSICION QUIMICA</subject><subject>COMPOSITION CHIMIQUE</subject><subject>ESTRUCTURA QUIMICA</subject><subject>FARMACOLOGIA</subject><subject>Female</subject><subject>Gas Chromatography-Mass Spectrometry</subject><subject>GLYCYRRHIZA</subject><subject>Hydrogen-Ion Concentration</subject><subject>Hydrolysis</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Mitogens - pharmacology</subject><subject>Molecular Sequence Data</subject><subject>PECTINAS</subject><subject>PECTINE</subject><subject>PHARMACOLOGIE</subject><subject>Plant Roots - chemistry</subject><subject>PLANTAS MEDICINALES</subject><subject>PLANTE MEDICINALE</subject><subject>POLISACARIDOS</subject><subject>Polygalacturonase - metabolism</subject><subject>POLYHOLOSIDE</subject><subject>Polysaccharides - chemistry</subject><subject>Polysaccharides - metabolism</subject><subject>Polysaccharides - pharmacology</subject><subject>RACINE</subject><subject>RAICES</subject><subject>STRUCTURE CHIMIQUE</subject><issn>0032-0943</issn><issn>1439-0221</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kUFv1DAQhS1EVZbCkQsSkk894WLHzibhBqUUpEr0AGfLmUyyrhx7sR2k7c_jl-FVVogLJ2tmPj_Nm0fIK8GvBK_rd4lVnG9ZVzdN2zwhG6Fkx3hViadkw7msGO-UfEaep_TAuVAd5-fkvG0qLluxIb_vEbIFug_ukAzAzkQ7YKJjDDONIeREw0hv3QEOMe7so6FLNA59suk9vQ8p2d4hheBztP2SbfBH3uOSC0aDs1P4V9Z6mndIJ-MM5CUGbxKyiEUtG59pxOmokAMtlWUQ5r3DGX028VBaA51tDhP6snD5b3_ZbDG9IGejcQlfnt4L8uPzzffrL-zu2-3X6w93DKRSmWFTDKMcxDD2HAFKyaHHFsDw1gxKjaYZOpACBtXWg-wLVY9KgNlWpVvJC3K56u5j-Llgynq2CdA54zEsSTdttVVCygKyFYRYDhRx1Pto52JBC66Pmemkj5npNbPCvzkJL_2Mw1_6FFKZv13neWfLNfRDWKIvTv8r93rFRxO0maJN-tNN13xs6q6TfwADo6_u</recordid><startdate>19960201</startdate><enddate>19960201</enddate><creator>Kiyohara, H</creator><creator>Takemoto, N</creator><creator>Zhao, J.F</creator><creator>Kawamura, H</creator><creator>Yamada, H. (Oriental Medicine Research Center of the Kitasato Institute, Tokyo (Japan))</creator><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19960201</creationdate><title>Pectic polysaccharides from roots of Glycyrrhiza uralensis: Possible contribution of neutral oligosaccharides in the galacturonase-resistant region to anti-complementary and mitogenic activities</title><author>Kiyohara, H ; Takemoto, N ; Zhao, J.F ; Kawamura, H ; Yamada, H. (Oriental Medicine Research Center of the Kitasato Institute, Tokyo (Japan))</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c344t-e7381e3d1dfb0ecc7380cbe8cca08ad44fa7d9c31cd485d3bb0e5f41ca62c3123</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Animals</topic><topic>Carbohydrate Sequence</topic><topic>Cells, Cultured</topic><topic>Complement Inactivator Proteins - pharmacology</topic><topic>COMPOSICION QUIMICA</topic><topic>COMPOSITION CHIMIQUE</topic><topic>ESTRUCTURA QUIMICA</topic><topic>FARMACOLOGIA</topic><topic>Female</topic><topic>Gas Chromatography-Mass Spectrometry</topic><topic>GLYCYRRHIZA</topic><topic>Hydrogen-Ion Concentration</topic><topic>Hydrolysis</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Mitogens - pharmacology</topic><topic>Molecular Sequence Data</topic><topic>PECTINAS</topic><topic>PECTINE</topic><topic>PHARMACOLOGIE</topic><topic>Plant Roots - chemistry</topic><topic>PLANTAS MEDICINALES</topic><topic>PLANTE MEDICINALE</topic><topic>POLISACARIDOS</topic><topic>Polygalacturonase - metabolism</topic><topic>POLYHOLOSIDE</topic><topic>Polysaccharides - chemistry</topic><topic>Polysaccharides - metabolism</topic><topic>Polysaccharides - pharmacology</topic><topic>RACINE</topic><topic>RAICES</topic><topic>STRUCTURE CHIMIQUE</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kiyohara, H</creatorcontrib><creatorcontrib>Takemoto, N</creatorcontrib><creatorcontrib>Zhao, J.F</creatorcontrib><creatorcontrib>Kawamura, H</creatorcontrib><creatorcontrib>Yamada, H. 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(Oriental Medicine Research Center of the Kitasato Institute, Tokyo (Japan))</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Pectic polysaccharides from roots of Glycyrrhiza uralensis: Possible contribution of neutral oligosaccharides in the galacturonase-resistant region to anti-complementary and mitogenic activities</atitle><jtitle>Planta medica</jtitle><addtitle>Planta Med</addtitle><date>1996-02-01</date><risdate>1996</risdate><volume>62</volume><issue>1</issue><spage>14</spage><epage>19</epage><pages>14-19</pages><issn>0032-0943</issn><eissn>1439-0221</eissn><abstract>Digestion with endo-alpha-(1-4)-polygalacturonase liberated the enzyme-resistant region (PG-1c) as an active site of the anti-complementary and mitogenic pectic polysaccharide (GR-22c) from Glycyrrhiza uralensis. Partial acid hydrolysis of PG-1c resulted in acidic oligosaccharides, and methylation analysis and malto-oligosaccharide-alditols, and malto-, GC-MS analysis of the acidic oligosaccharides suggested that PG-1c comprised a rhamnogalacturonan core as the acidic moiety. Degradation of uronic acids by lithium decreased the anti-complementary and mitogenic activities of PG-1c. Although the products from PG-1c were still active, the methylglycoside of alpha-L-Rha-(1-4)-alpha-D-GalA-(1-2)-alpha-L-Rha-(1-4)-alpha -D-GalA did not show both activities. The products obtained by the lithium degradation from PG-1c gave fractions containing various neutral oligosaccharide-alditols. Among these fractions the longest and the short oligosaccharide-alditol fraktions had relatively potent anti-complementary activity, whereas all oligosaccharide-alditol fractions expressed weak but significant mitogenic activity. GC-MS analysis indicated that the short oligosaccharide-alditol fraction contained various kinds of di- to tetrasaccharide-alditols. However, malto-oligosaccharide-alditols, and malto-, isomalto-, and laminari-oligosaccharides did not show anti-complementary and/or mitogenic activities, and these results suggested that certain neutral carbohydrate chains in PG-1c were responsible for the expression of mitogenic activity as well as anti-complementary activity of PG-1c.</abstract><cop>Germany</cop><pmid>8720381</pmid><doi>10.1055/s-2006-957787</doi><tpages>6</tpages></addata></record> |
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| source | MEDLINE; Thieme Connect Journals |
| subjects | Animals Carbohydrate Sequence Cells, Cultured Complement Inactivator Proteins - pharmacology COMPOSICION QUIMICA COMPOSITION CHIMIQUE ESTRUCTURA QUIMICA FARMACOLOGIA Female Gas Chromatography-Mass Spectrometry GLYCYRRHIZA Hydrogen-Ion Concentration Hydrolysis Mice Mice, Inbred BALB C Mitogens - pharmacology Molecular Sequence Data PECTINAS PECTINE PHARMACOLOGIE Plant Roots - chemistry PLANTAS MEDICINALES PLANTE MEDICINALE POLISACARIDOS Polygalacturonase - metabolism POLYHOLOSIDE Polysaccharides - chemistry Polysaccharides - metabolism Polysaccharides - pharmacology RACINE RAICES STRUCTURE CHIMIQUE |
| title | Pectic polysaccharides from roots of Glycyrrhiza uralensis: Possible contribution of neutral oligosaccharides in the galacturonase-resistant region to anti-complementary and mitogenic activities |
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