A one-step sandwich enzyme immunoassay for human matrix metalloproteinase 8 (neutrophil collagenase) using monoclonal antibodies

A one-step sandwich enzyme immunoassay (EIA) system for human matrix metalloproteinase 8 (MMP-8, neutrophil collagenase, EC 3.4.24.7) has been established with a pair of monoclonal antibodies prepared against the zymogen of MMP-8 purified from human neutrophils. MMP-8 in samples simultaneously react...

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Veröffentlicht in:	Clinica chimica acta 1996-01, Vol.244 (2), p.129-143
Hauptverfasser:	Matsuki, Hirokazu, Fujimoto, Noboru, Iwata, Kazushi, Knäuper, Vera, Okada, Yasunori, Hayakawa, Taro
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antibodies, Monoclonal Antibody Specificity Arthritis, Rheumatoid - blood Arthritis, Rheumatoid - enzymology Biological and medical sciences Biomarkers Cell Line Chromatography, Affinity Chromatography, Gel Collagenases - analysis Collagenases - blood Collagenases - isolation & purification Diseases of the osteoarticular system Electrophoresis, Polyacrylamide Gel Female Humans Immunoenzyme Techniques Immunohistochemistry Investigative techniques, diagnostic techniques (general aspects) Matrix Metalloproteinase 8 Medical sciences Mice Mice, Inbred BALB C Miscellaneous. Osteoarticular involvement in other diseases Molecular Weight Monoclonal antibody Neutrophils - enzymology Osteoarthritis - blood Osteoarthritis - enzymology Osteoarticular system. Muscles Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques Placenta - enzymology Pregnancy Saliva - enzymology Sandwich enzyme immunoassay Statistics, Nonparametric Stomach Neoplasms - enzymology Stomach Neoplasms - pathology Stomach Neoplasms - surgery Synovial Fluid - enzymology Tissue inhibitor of metalloproteinases Tumor Cells, Cultured
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