Persistence and replication of plasmid DNA microinjected into early embryos of Xenopus laevis

The persistence and replication of defined circular and linear plasmid DNA molecules microinjected into fertilized eggs of Xenopus laevis were analyzed. For all plasmids tested, a small fraction of microinjected circular molecules was replicated; however, the overall copy numbers of either free form...

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Veröffentlicht in:	Developmental biology 1988-06, Vol.127 (2), p.421-434
Hauptverfasser:	Marini, Nicholas J., Etkin, Laurence D., Benbow, Robert M.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biological and medical sciences Biotechnology Cleavage Stage, Ovum - physiology DNA Replication DNA, Circular - physiology Fundamental and applied biological sciences. Psychology Genetic engineering Genetic technics Methods. Procedures. Technologies Microinjections Molecular Weight Plasmids Vectors (cloning, transfer, expression). Insertion sequences and transposons Xenopus laevis Zygote - physiology
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